UniProtKB - O35987 (NSF1C_RAT)
NSFL1 cofactor p47
Nsfl1c
Functioni
GO - Molecular functioni
- ATPase binding Source: RGD
- lipid binding Source: UniProtKB-KW
- ubiquitin binding Source: BHF-UCL
GO - Biological processi
- autophagosome assembly Source: GO_Central
- establishment of mitotic spindle orientation Source: RGD
- Golgi organization Source: RGD
- membrane fusion Source: RGD
- negative regulation of protein localization to centrosome Source: RGD
- nuclear envelope reassembly Source: GO_Central
- positive regulation of mitotic centrosome separation Source: RGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: NSFL1 cofactor p47Alternative name(s): XY body-associated protein XY40 p97 cofactor p47 |
Gene namesi | Name:Nsfl1c |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619952, Nsfl1c |
Subcellular locationi
Cytoskeleton
- centrosome 1 Publication
Golgi apparatus
- Golgi stack 2 Publications
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Note: Predominantly nuclear in interphase cells (PubMed:12810701). Bound to the axial elements of sex chromosomes in pachytene spermatocytes (PubMed:1495983). A small proportion of the protein is cytoplasmic, associated with Golgi stacks (PubMed:12810701). Localizes to centrosome during mitotic prophase and metaphase (PubMed:23649807).3 Publications
Cytoskeleton
- intermediate filament cytoskeleton Source: Ensembl
- spindle pole centrosome Source: UniProtKB
Cytosol
- cytosol Source: ParkinsonsUK-UCL
Golgi apparatus
- Golgi stack Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: Ensembl
Other locations
- chromosome Source: UniProtKB-SubCell
- VCP-NSFL1C complex Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 41 | F → A: Reduces ubiquitin binding and Golgi reassembly. 1 Publication | 1 | |
Mutagenesisi | 57 | T → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 112 | K → T: Strongly reduces nuclear location. 1 Publication | 1 | |
Mutagenesisi | 114 | S → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 140 | S → A: Abolishes phosphorylation by CDK1. 1 Publication | 1 | |
Mutagenesisi | 140 | S → D: Strongly reduces binding to Golgi membranes. 1 Publication | 1 | |
Mutagenesisi | 173 | R → T: Strongly reduces nuclear location. 1 Publication | 1 | |
Mutagenesisi | 272 | S → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 301 | R → A: Reduced interaction with VCP. 1 Publication | 1 | |
Mutagenesisi | 342 – 345 | TFPN → AG: Strongly reduced interaction with VCP. 1 Publication | 4 | |
Mutagenesisi | 343 | F → S: Reduced interaction with VCP. 1 Publication | 1 | |
Mutagenesisi | 345 | N → A: Reduced interaction with VCP. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210990 | 1 – 370 | NSFL1 cofactor p47Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 74 | PhosphoserineBy similarity | 1 | |
Modified residuei | 102 | PhosphoserineBy similarity | 1 | |
Modified residuei | 114 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | Phosphoserine; by CDK11 Publication | 1 | |
Modified residuei | 167 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 176 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineBy similarity | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O35987 |
PaxDbi | O35987 |
PRIDEi | O35987 |
PTM databases
iPTMneti | O35987 |
PhosphoSitePlusi | O35987 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSRNOG00000008604, Expressed in female gonad and 21 other tissues |
ExpressionAtlasi | O35987, baseline and differential |
Genevisiblei | O35987, RN |
Interactioni
Subunit structurei
Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP.
8 PublicationsBinary interactionsi
Hide detailsO35987
With | #Exp. | IntAct |
---|---|---|
Vcp [P46462] | 12 | EBI-1993760,EBI-399011 |
Vcp [Q01853] from Mus musculus. | 8 | EBI-1993760,EBI-80597 |
GO - Molecular functioni
- ATPase binding Source: RGD
- ubiquitin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 249844, 4 interactors |
ComplexPortali | CPX-263, Nsfl1c-Vcp complex |
CORUMi | O35987 |
IntActi | O35987, 18 interactors |
MINTi | O35987 |
STRINGi | 10116.ENSRNOP00000011654 |
Structurei
Secondary structure
3D structure databases
BMRBi | O35987 |
SMRi | O35987 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O35987 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 179 – 244 | SEPPROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 291 – 368 | UBXPROSITE-ProRule annotationAdd BLAST | 78 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 109 – 115 | Nuclear localization signal | 7 | |
Motifi | 172 – 175 | Nuclear localization signal | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2086, Eukaryota |
GeneTreei | ENSGT00520000055567 |
InParanoidi | O35987 |
OMAi | ERVLHIW |
OrthoDBi | 1175850at2759 |
PhylomeDBi | O35987 |
Family and domain databases
Gene3Di | 3.30.420.210, 1 hit |
IDEALi | IID50026 |
InterProi | View protein in InterPro IPR036241, NSFL1C_SEP_dom_sf IPR012989, SEP_domain IPR009060, UBA-like_sf IPR029071, Ubiquitin-like_domsf IPR001012, UBX_dom |
Pfami | View protein in Pfam PF08059, SEP, 1 hit PF00789, UBX, 1 hit |
SMARTi | View protein in SMART SM00553, SEP, 1 hit SM00166, UBX, 1 hit |
SUPFAMi | SSF102848, SSF102848, 1 hit SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51399, SEP, 1 hit PS50033, UBX, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAEERQDALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI
60 70 80 90 100
VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEEEEEEE GQRFYAGGSE
110 120 130 140 150
RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF
160 170 180 190 200
AGGGYRLGAA PEEESAYVAG ERRRHSGQDV HVVLKLWKTG FSLDNGDLRS
210 220 230 240 250
YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF
260 270 280 290 300
KAFTGEGQKL GSTAPQVLNT SSPAQQAENE AKASSSILIN EAEPTTNIQI
310 320 330 340 350
RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFVLM TTFPNKELAD
360 370
ENQTLKEANL LNAVIVQRLT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K911 | A0A0G2K911_RAT | NSFL1 cofactor p47 | Nsfl1c | 340 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10769 mRNA Translation: CAA71742.1 AB002086 mRNA Translation: BAA21659.1 BC072464 mRNA Translation: AAH72464.1 |
RefSeqi | NP_114187.1, NM_031981.2 |
Genome annotation databases
Ensembli | ENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604 |
GeneIDi | 83809 |
KEGGi | rno:83809 |
UCSCi | RGD:619952, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10769 mRNA Translation: CAA71742.1 AB002086 mRNA Translation: BAA21659.1 BC072464 mRNA Translation: AAH72464.1 |
RefSeqi | NP_114187.1, NM_031981.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I42 | NMR | - | A | 282-370 | [»] | |
1JRU | NMR | - | A | 282-370 | [»] | |
1S3S | X-ray | 2.90 | G/H/I | 244-370 | [»] | |
1V92 | NMR | - | A | 1-46 | [»] | |
1VAZ | NMR | - | A | 171-246 | [»] | |
BMRBi | O35987 | |||||
SMRi | O35987 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 249844, 4 interactors |
ComplexPortali | CPX-263, Nsfl1c-Vcp complex |
CORUMi | O35987 |
IntActi | O35987, 18 interactors |
MINTi | O35987 |
STRINGi | 10116.ENSRNOP00000011654 |
PTM databases
iPTMneti | O35987 |
PhosphoSitePlusi | O35987 |
Proteomic databases
jPOSTi | O35987 |
PaxDbi | O35987 |
PRIDEi | O35987 |
Genome annotation databases
Ensembli | ENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604 |
GeneIDi | 83809 |
KEGGi | rno:83809 |
UCSCi | RGD:619952, rat |
Organism-specific databases
CTDi | 55968 |
RGDi | 619952, Nsfl1c |
Phylogenomic databases
eggNOGi | KOG2086, Eukaryota |
GeneTreei | ENSGT00520000055567 |
InParanoidi | O35987 |
OMAi | ERVLHIW |
OrthoDBi | 1175850at2759 |
PhylomeDBi | O35987 |
Miscellaneous databases
EvolutionaryTracei | O35987 |
PROi | PR:O35987 |
Gene expression databases
Bgeei | ENSRNOG00000008604, Expressed in female gonad and 21 other tissues |
ExpressionAtlasi | O35987, baseline and differential |
Genevisiblei | O35987, RN |
Family and domain databases
Gene3Di | 3.30.420.210, 1 hit |
IDEALi | IID50026 |
InterProi | View protein in InterPro IPR036241, NSFL1C_SEP_dom_sf IPR012989, SEP_domain IPR009060, UBA-like_sf IPR029071, Ubiquitin-like_domsf IPR001012, UBX_dom |
Pfami | View protein in Pfam PF08059, SEP, 1 hit PF00789, UBX, 1 hit |
SMARTi | View protein in SMART SM00553, SEP, 1 hit SM00166, UBX, 1 hit |
SUPFAMi | SSF102848, SSF102848, 1 hit SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51399, SEP, 1 hit PS50033, UBX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NSF1C_RAT | |
Accessioni | O35987Primary (citable) accession number: O35987 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families