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Protein

Guanidinoacetate N-methyltransferase

Gene

Gamt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.PROSITE-ProRule annotation

Pathwayi: creatine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (Gatm)
  2. Guanidinoacetate N-methyltransferase (Gamt)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei42SubstratePROSITE-ProRule annotation1
Binding sitei46SubstratePROSITE-ProRule annotation1
Binding sitei50S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei135S-adenosyl-L-methionine and substratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-71288 Creatine metabolism
UniPathwayi
UPA00104;UER00580

Names & Taxonomyi

Protein namesi
Recommended name:
Guanidinoacetate N-methyltransferase (EC:2.1.1.2)
Gene namesi
Name:Gamt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1098221 Gamt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000874312 – 236Guanidinoacetate N-methyltransferaseAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei7PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35969
PaxDbiO35969
PeptideAtlasiO35969
PRIDEiO35969

2D gel databases

REPRODUCTION-2DPAGEiIPI00742399
O35969
SWISS-2DPAGEiO35969

PTM databases

iPTMnetiO35969
PhosphoSitePlusiO35969
SwissPalmiO35969

Expressioni

Tissue specificityi

Highly expressed in testis, caput epididymis, ovary, and liver. In the testis, localized primarily in Sertoli cells. Expressed in brain with high levels in oligodendrocytes and olfactory ensheathing glia. Moderate levels of expression in astrocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000020150 Expressed in 194 organ(s), highest expression level in pancreas
CleanExiMM_GAMT
GenevisibleiO35969 MM

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35969, 2 interactors
MINTiO35969
STRINGi10090.ENSMUSP00000101002

Structurei

3D structure databases

ProteinModelPortaliO35969
SMRiO35969
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 236RMT2PROSITE-ProRule annotationAdd BLAST224

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 74S-adenosyl-L-methionine bindingPROSITE-ProRule annotation6
Regioni90 – 92S-adenosyl-L-methioninePROSITE-ProRule annotation3
Regioni117 – 118S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2
Regioni171 – 172Substrate bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ISNM Eukaryota
ENOG410XPX4 LUCA
GeneTreeiENSGT00390000018061
HOGENOMiHOG000010290
HOVERGENiHBG005801
InParanoidiO35969
KOiK00542
OMAiNHFDGIL
OrthoDBiEOG091G0EPW
PhylomeDBiO35969
TreeFamiTF328555

Family and domain databases

InterProiView protein in InterPro
IPR016550 GuanidinoAc_N-MeTrfase
IPR026480 RMT2_dom
IPR029063 SAM-dependent_MTases
PIRSFiPIRSF009285 GAMT, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51559 SAM_RMT2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O35969-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM
60 70 80 90 100
HALAAAAASR GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ
110 120 130 140 150
DWALRQPHKV VPLKGLWEEV APTLPDGHFD GILYDTYPLS EEAWHTHQFN
160 170 180 190 200
FIKNHAFRLL KTGGVLTYCN LTSWGELMKS KYTDITTMFE ETQVPALQEA
210 220 230
GFLKENICTE VMALVPPADC RYYAFPQMIT PLVTKH
Length:236
Mass (Da):26,336
Last modified:January 1, 1998 - v1
Checksum:i3D982DE5D51DF5CD
GO
Isoform 2 (identifier: O35969-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-153: K → KLSSHGPTPSCPLASLQ

Note: No experimental confirmation available.
Show »
Length:252
Mass (Da):27,913
Checksum:i2F7006B71CFA8FF1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017727153K → KLSSHGPTPSCPLASLQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015887 mRNA Translation: AAB81495.1
AF010499, AF010498 Genomic DNA Translation: AAB81498.1
AK140688 mRNA Translation: BAE24443.1
AK158087 mRNA Translation: BAE34352.1
BC049233 mRNA Translation: AAH49233.1
CCDSiCCDS35976.1 [O35969-1]
CCDS83728.1 [O35969-2]
PIRiJC5664
RefSeqiNP_001334048.1, NM_001347119.1 [O35969-2]
NP_034385.1, NM_010255.4 [O35969-1]
UniGeneiMm.7329

Genome annotation databases

EnsembliENSMUST00000020359; ENSMUSP00000020359; ENSMUSG00000020150 [O35969-2]
ENSMUST00000105363; ENSMUSP00000101002; ENSMUSG00000020150 [O35969-1]
GeneIDi14431
KEGGimmu:14431
UCSCiuc007gcj.2 mouse [O35969-1]
uc011xio.1 mouse [O35969-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015887 mRNA Translation: AAB81495.1
AF010499, AF010498 Genomic DNA Translation: AAB81498.1
AK140688 mRNA Translation: BAE24443.1
AK158087 mRNA Translation: BAE34352.1
BC049233 mRNA Translation: AAH49233.1
CCDSiCCDS35976.1 [O35969-1]
CCDS83728.1 [O35969-2]
PIRiJC5664
RefSeqiNP_001334048.1, NM_001347119.1 [O35969-2]
NP_034385.1, NM_010255.4 [O35969-1]
UniGeneiMm.7329

3D structure databases

ProteinModelPortaliO35969
SMRiO35969
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35969, 2 interactors
MINTiO35969
STRINGi10090.ENSMUSP00000101002

PTM databases

iPTMnetiO35969
PhosphoSitePlusiO35969
SwissPalmiO35969

2D gel databases

REPRODUCTION-2DPAGEiIPI00742399
O35969
SWISS-2DPAGEiO35969

Proteomic databases

MaxQBiO35969
PaxDbiO35969
PeptideAtlasiO35969
PRIDEiO35969

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020359; ENSMUSP00000020359; ENSMUSG00000020150 [O35969-2]
ENSMUST00000105363; ENSMUSP00000101002; ENSMUSG00000020150 [O35969-1]
GeneIDi14431
KEGGimmu:14431
UCSCiuc007gcj.2 mouse [O35969-1]
uc011xio.1 mouse [O35969-2]

Organism-specific databases

CTDi2593
MGIiMGI:1098221 Gamt

Phylogenomic databases

eggNOGiENOG410ISNM Eukaryota
ENOG410XPX4 LUCA
GeneTreeiENSGT00390000018061
HOGENOMiHOG000010290
HOVERGENiHBG005801
InParanoidiO35969
KOiK00542
OMAiNHFDGIL
OrthoDBiEOG091G0EPW
PhylomeDBiO35969
TreeFamiTF328555

Enzyme and pathway databases

UniPathwayi
UPA00104;UER00580

ReactomeiR-MMU-71288 Creatine metabolism

Miscellaneous databases

PROiPR:O35969
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020150 Expressed in 194 organ(s), highest expression level in pancreas
CleanExiMM_GAMT
GenevisibleiO35969 MM

Family and domain databases

InterProiView protein in InterPro
IPR016550 GuanidinoAc_N-MeTrfase
IPR026480 RMT2_dom
IPR029063 SAM-dependent_MTases
PIRSFiPIRSF009285 GAMT, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51559 SAM_RMT2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGAMT_MOUSE
AccessioniPrimary (citable) accession number: O35969
Secondary accession number(s): Q3TZ58, Q3US90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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