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Protein

Serine/threonine-protein kinase Nek2

Gene

Nek2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (By similarity). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (By similarity). Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction (By similarity).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Activity regulationi

Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATPPROSITE-ProRule annotation1
Active sitei141Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek2 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 2
Short name:
NimA-related protein kinase 2
Gene namesi
Name:Nek2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:109359 Nek2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864221 – 443Serine/threonine-protein kinase Nek2Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Phosphothreonine; by autocatalysisBy similarity1
Modified residuei171Phosphoserine; by autocatalysisBy similarity1
Modified residuei175Phosphothreonine; by autocatalysisBy similarity1
Modified residuei179Phosphothreonine; by autocatalysisBy similarity1
Modified residuei184PhosphoserineBy similarity1
Modified residuei241Phosphoserine; by autocatalysisBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei356Phosphoserine; by STK3/MST2By similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei396PhosphoserineBy similarity1
Modified residuei401PhosphoserineBy similarity1
Modified residuei436Phosphoserine; by STK3/MST2By similarity1

Post-translational modificationi

Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO35942
PaxDbiO35942
PRIDEiO35942

PTM databases

iPTMnetiO35942
PhosphoSitePlusiO35942

Expressioni

Tissue specificityi

Most abundantly expressed in testis. Low levels found in mid-gestation embryo, ovary, placenta, intestine, thymus and skin. Within the testis, expression restricted to germ cells with highest levels detected in spermatocytes at pachytene and diplotene stages. Also expressed in meiotic pachytene oocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000026622 Expressed in 195 organ(s), highest expression level in testis
GenevisibleiO35942 MM

Interactioni

Subunit structurei

Forms homodimers and heterodimers. Interacts with CDC20, CTNB1, MAD1L1, MAD2L1, MAPK, NEK11, NPM1, NDC80, PCNT, PPP1CA, PPP1CC and SGO1. Interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain) (By similarity). Interacts with NECAB3 and HMGA2 (PubMed:14697346, PubMed:14668482). Interacts with CEP68; the interaction leads to phosphorylation of CEP68. Interacts with CNTLN; the interaction leads to phosphorylation of CNTLN. Interacts with CEP85 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201728, 38 interactors
IntActiO35942, 37 interactors
STRINGi10090.ENSMUSP00000027931

Structurei

3D structure databases

ProteinModelPortaliO35942
SMRiO35942
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 271Protein kinasePROSITE-ProRule annotationAdd BLAST264

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 443Interaction with PCNTBy similarityAdd BLAST180
Regioni301 – 443Interaction with CEP85By similarityAdd BLAST143
Regioni306 – 334Leucine-zipperAdd BLAST29
Regioni329 – 443Necessary for interaction with MAD1L1By similarityAdd BLAST115
Regioni333 – 370Required for microtubule binding and for localization to the centrosomesBy similarityAdd BLAST38
Regioni402 – 437Interaction with SAV1 and STK3/MST2By similarityAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili303 – 361Sequence analysisAdd BLAST59
Coiled coili403 – 427Sequence analysisAdd BLAST25

Domaini

The leucine-zipper domain is required for its dimerization and activation.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0591 Eukaryota
ENOG410XNQP LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000233029
HOVERGENiHBG006461
InParanoidiO35942
KOiK20872
OMAiAYNQKEL
OrthoDBiEOG091G07PD
TreeFamiTF101184

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O35942-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSRVEDYEV LHSIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEVEKQ
60 70 80 90 100
MLVSEVNLLR ELKHPNIVSY YDRIIDRTNT TLYIVMEYCE GGDLASVISK
110 120 130 140 150
GTKDRQYLEE EFVLRVMTQL TLALKECHRR SDGGHTVLHR DLKPANVFLD
160 170 180 190 200
SKHNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQMSC LSYNEKSDIW
210 220 230 240 250
SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR
260 270 280 290 300
MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLPDSS
310 320 330 340 350
PVLSELKLKE RQLQDREQAL RAREDILEQK ERELCIRERL AEDKLARAES
360 370 380 390 400
LMKNYSLLKE HRLLCLAGGP ELDLPSSAMK KKVHFHGESK ENTARSENSE
410 420 430 440
SYLAKSKCRD LKKRLHAAQL RAQALADIEK NYQLKSRQIL GMR
Length:443
Mass (Da):51,243
Last modified:July 27, 2011 - v2
Checksum:i1EF2CA320F60FB5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69S → R in AAB67973 (PubMed:9187143).Curated1
Sequence conflicti69S → R in AAC35393 (PubMed:9583679).Curated1
Sequence conflicti69S → R in AAB70470 (PubMed:9434622).Curated1
Sequence conflicti203G → A in AAB67973 (PubMed:9187143).Curated1
Sequence conflicti253N → F in AAB67973 (PubMed:9187143).Curated1
Sequence conflicti274L → M in AAB67973 (PubMed:9187143).Curated1
Sequence conflicti274L → M in AAC35393 (PubMed:9583679).Curated1
Sequence conflicti274L → M in AAB70470 (PubMed:9434622).Curated1
Sequence conflicti311R → S in AAB67973 (PubMed:9187143).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95610 mRNA Translation: AAB67973.1
AF013166 mRNA Translation: AAC35393.1
AF007247 mRNA Translation: AAB70470.1
AK147072 mRNA Translation: BAE27653.1
AK164467 mRNA Translation: BAE37799.1
CCDSiCCDS15623.1
RefSeqiNP_035022.2, NM_010892.3
UniGeneiMm.33773
Mm.403999

Genome annotation databases

EnsembliENSMUST00000027931; ENSMUSP00000027931; ENSMUSG00000026622
ENSMUST00000213394; ENSMUSP00000149034; ENSMUSG00000110797
GeneIDi18005
KEGGimmu:18005
UCSCiuc007ecx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95610 mRNA Translation: AAB67973.1
AF013166 mRNA Translation: AAC35393.1
AF007247 mRNA Translation: AAB70470.1
AK147072 mRNA Translation: BAE27653.1
AK164467 mRNA Translation: BAE37799.1
CCDSiCCDS15623.1
RefSeqiNP_035022.2, NM_010892.3
UniGeneiMm.33773
Mm.403999

3D structure databases

ProteinModelPortaliO35942
SMRiO35942
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201728, 38 interactors
IntActiO35942, 37 interactors
STRINGi10090.ENSMUSP00000027931

PTM databases

iPTMnetiO35942
PhosphoSitePlusiO35942

Proteomic databases

MaxQBiO35942
PaxDbiO35942
PRIDEiO35942

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027931; ENSMUSP00000027931; ENSMUSG00000026622
ENSMUST00000213394; ENSMUSP00000149034; ENSMUSG00000110797
GeneIDi18005
KEGGimmu:18005
UCSCiuc007ecx.2 mouse

Organism-specific databases

CTDi4751
MGIiMGI:109359 Nek2

Phylogenomic databases

eggNOGiKOG0591 Eukaryota
ENOG410XNQP LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000233029
HOVERGENiHBG006461
InParanoidiO35942
KOiK20872
OMAiAYNQKEL
OrthoDBiEOG091G07PD
TreeFamiTF101184

Enzyme and pathway databases

ReactomeiR-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

PROiPR:O35942
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026622 Expressed in 195 organ(s), highest expression level in testis
GenevisibleiO35942 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNEK2_MOUSE
AccessioniPrimary (citable) accession number: O35942
Secondary accession number(s): O35959, Q3TPD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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