UniProtKB - O35921 (EAA4_RAT)
Excitatory amino acid transporter 4
Slc1a6
Functioni
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:14506254, PubMed:26690923).
Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (PubMed:14506254).
Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport (PubMed:14506254).
Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).
Curated2 PublicationsKineticsi
- KM=328 µM for L-glutamate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 416 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 418 | Sodium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 420 | Sodium 1By similarity | 1 | |
Binding sitei | 424 | AspartateBy similarity | 1 | |
Binding sitei | 498 | AspartateBy similarity | 1 | |
Metal bindingi | 505 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 505 | AspartateBy similarity | 1 | |
Metal bindingi | 509 | Sodium 1By similarity | 1 |
GO - Molecular functioni
- anion transmembrane transporter activity Source: RGD
- glutamate:sodium symporter activity Source: GO_Central
- high-affinity glutamate transmembrane transporter activity Source: UniProtKB
- L-glutamate transmembrane transporter activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- neutral amino acid transmembrane transporter activity Source: GO_Central
GO - Biological processi
- L-glutamate import across plasma membrane Source: UniProtKB
- L-glutamate transmembrane transport Source: UniProtKB
- neurotransmitter uptake Source: RGD
- regulation of membrane potential Source: RGD
Keywordsi
Biological process | Amino-acid transport, Symport, Transport |
Ligand | Chloride, Metal-binding, Potassium, Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-210500, Glutamate Neurotransmitter Release Cycle R-RNO-425393, Transport of inorganic cations/anions and amino acids/oligopeptides |
Names & Taxonomyi
Protein namesi | Recommended name: Excitatory amino acid transporter 4Alternative name(s): High-affinity neuronal glutamate transporter Sodium-dependent glutamate/aspartate transporter Solute carrier family 1 member 6 |
Gene namesi | Name:Slc1a6 Synonyms:Eaat4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620340, Slc1a6 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Curated
Golgi apparatus
- Golgi apparatus Source: RGD
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of postsynaptic membrane Source: SynGO
- integral component of presynaptic membrane Source: RGD
- plasma membrane Source: GO_Central
Other locations
- glutamatergic synapse Source: RGD
- membrane Source: RGD
- membrane protein complex Source: ARUK-UCL
- parallel fiber to Purkinje cell synapse Source: SynGO
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 52 | CytoplasmicSequence analysisAdd BLAST | 52 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 96 – 116 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 130 – 150 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 259 – 282 | Helical; Name=4By similarityAdd BLAST | 24 | |
Transmembranei | 292 – 319 | Helical; Name=5By similarityAdd BLAST | 28 | |
Transmembranei | 341 – 362 | Helical; Name=6By similarityAdd BLAST | 22 | |
Intramembranei | 368 – 398 | Discontinuously helicalBy similarityAdd BLAST | 31 | |
Transmembranei | 408 – 434 | Helical; Name=7By similarityAdd BLAST | 27 | |
Intramembranei | 448 – 481 | Discontinuously helicalBy similarityAdd BLAST | 34 | |
Transmembranei | 495 – 516 | Helical; Name=8By similarityAdd BLAST | 22 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202072 | 1 – 561 | Excitatory amino acid transporter 4Add BLAST | 561 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 213 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 236 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | O35921 |
PRIDEi | O35921 |
PTM databases
GlyGeni | O35921, 3 sites |
iPTMneti | O35921 |
PhosphoSitePlusi | O35921 |
Interactioni
Subunit structurei
Homotrimer.
CuratedProtein-protein interaction databases
BioGRIDi | 249878, 2 interactors |
IntActi | O35921, 1 interactor |
MINTi | O35921 |
STRINGi | 10116.ENSRNOP00000009931 |
Chemistry databases
BindingDBi | O35921 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 385 – 387 | Aspartate bindingBy similarity | 3 | |
Regioni | 465 – 469 | Aspartate bindingBy similarity | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3787, Eukaryota |
InParanoidi | O35921 |
PhylomeDBi | O35921 |
Family and domain databases
Gene3Di | 1.10.3860.10, 1 hit |
InterProi | View protein in InterPro IPR001991, Na-dicarboxylate_symporter IPR018107, Na-dicarboxylate_symporter_CS IPR036458, Na:dicarbo_symporter_sf |
Pfami | View protein in Pfam PF00375, SDF, 1 hit |
SUPFAMi | SSF118215, SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713, NA_DICARBOXYL_SYMP_1, 1 hit PS00714, NA_DICARBOXYL_SYMP_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSSHGNSLFL RESGAGGGCL QGLQDSLQQR ALRTRLRLQT MTREHVRRFL
60 70 80 90 100
RRNAFILLTV SAVIIGVSLA FALRPYQLTY RQIKYFSFPG ELLMRMLQML
110 120 130 140 150
VLPLIVSSRV TGMASLDNKA TGRMGMRAAV YYMVTTVIAV FIGILMVTII
160 170 180 190 200
HPGKGSKEGL HREGRIETVP TADAFMDLVR NMFPPNLVEA CFKQFKTQYS
210 220 230 240 250
TRVVTRTIVR TDNGSELGAS ISPPSSAENE TSILENVTRA LGTLQEVISF
260 270 280 290 300
EETVPVPGSA NGINALGLVV FSVAFGLVIG GMKHKGRVLR DFFDSLNEAI
310 320 330 340 350
MRLVGIIIWY APVGILFLIA GKILEMEDMA VLGGQLGMYT LTVIVGLFLH
360 370 380 390 400
AGGVLPLIYF LVTHRNPFPF IGGILQALIT AMGTSSSSAT LPITFRCLEE
410 420 430 440 450
GLGVDRRITR FVLPVGATVN MDGTALYEAL AAIFIAQVNN YELNLGQITT
460 470 480 490 500
ISITATAASV GAAGIPQAGL VTMVIVLTSV GLPTEDITLI IAVDWFLDRL
510 520 530 540 550
RTMTNVLGDS IGAAVIEHLS QRELELQEAE LTLPSLGKPY KSLMAQAKGA
560
SRGRGGNESV M
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ7TSX6 | Q7TSX6_RAT | Amino acid transporter | Slc1a6 | 561 | Annotation score: | ||
F1LR26 | F1LR26_RAT | Amino acid transporter | Slc1a6 | 558 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | K → R in AAB40997 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89608 mRNA Translation: AAB72086.1 U80915 mRNA Translation: AAB40997.1 |
RefSeqi | NP_114454.1, NM_032065.1 |
Genome annotation databases
GeneIDi | 84012 |
KEGGi | rno:84012 |
UCSCi | RGD:620340, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89608 mRNA Translation: AAB72086.1 U80915 mRNA Translation: AAB40997.1 |
RefSeqi | NP_114454.1, NM_032065.1 |
3D structure databases
AlphaFoldDBi | O35921 |
SMRi | O35921 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249878, 2 interactors |
IntActi | O35921, 1 interactor |
MINTi | O35921 |
STRINGi | 10116.ENSRNOP00000009931 |
Chemistry databases
BindingDBi | O35921 |
ChEMBLi | CHEMBL3700 |
GuidetoPHARMACOLOGYi | 871 |
PTM databases
GlyGeni | O35921, 3 sites |
iPTMneti | O35921 |
PhosphoSitePlusi | O35921 |
Proteomic databases
PaxDbi | O35921 |
PRIDEi | O35921 |
Genome annotation databases
GeneIDi | 84012 |
KEGGi | rno:84012 |
UCSCi | RGD:620340, rat |
Organism-specific databases
CTDi | 6511 |
RGDi | 620340, Slc1a6 |
Phylogenomic databases
eggNOGi | KOG3787, Eukaryota |
InParanoidi | O35921 |
PhylomeDBi | O35921 |
Enzyme and pathway databases
Reactomei | R-RNO-210500, Glutamate Neurotransmitter Release Cycle R-RNO-425393, Transport of inorganic cations/anions and amino acids/oligopeptides |
Miscellaneous databases
PROi | PR:O35921 |
Family and domain databases
Gene3Di | 1.10.3860.10, 1 hit |
InterProi | View protein in InterPro IPR001991, Na-dicarboxylate_symporter IPR018107, Na-dicarboxylate_symporter_CS IPR036458, Na:dicarbo_symporter_sf |
Pfami | View protein in Pfam PF00375, SDF, 1 hit |
SUPFAMi | SSF118215, SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713, NA_DICARBOXYL_SYMP_1, 1 hit PS00714, NA_DICARBOXYL_SYMP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EAA4_RAT | |
Accessioni | O35921Primary (citable) accession number: O35921 Secondary accession number(s): P97683 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families