UniProtKB - O35914 (BNC1_MOUSE)
Protein
Zinc finger protein basonuclin-1
Gene
Bnc1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional activator (PubMed:9687312, PubMed:23707421). Likely specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation (By similarity). Required for the maintenance of spermatogenesis (PubMed:22266914). May also play a role in the differentiation of oocytes and the early development of embryos (PubMed:9687312).By similarity3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 325 – 348 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 353 – 382 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 687 – 711 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 715 – 743 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 895 – 918 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 923 – 950 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- chromosome organization Source: MGI
- positive regulation of epithelial cell proliferation Source: MGI
- regulation of transcription by RNA polymerase I Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- spermatogenesis Source: UniProtKB-KW
- wound healing Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Differentiation, Spermatogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein basonuclin-1 |
Gene namesi | Name:Bnc1 Synonyms:Bnc |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097164 Bnc1 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
- nucleoplasm 1 Publication
Other locations
- Cytoplasm 2 Publications
Note: Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.1 Publication
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Viable, although fertility is severely reduced in both males and females. Males have significantly reduced testis size, with progressive degeneration of seminiferous epithelium and loss of germ cells from 8 weeks of age. Sperm motility is also reduced.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046933 | 1 – 961 | Zinc finger protein basonuclin-1Add BLAST | 961 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 505 | PhosphoserineBy similarity | 1 | |
Modified residuei | 509 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation on Ser-505 and Ser-509 leads to cytoplasmic localization.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O35914 |
PRIDEi | O35914 |
PTM databases
iPTMneti | O35914 |
PhosphoSitePlusi | O35914 |
Expressioni
Tissue specificityi
Epidermis and germ cells of testis and ovary.1 Publication
Developmental stagei
Appears to be expressed by primary and secondary spermatocytes and spermatids. Detected in oocytes within the primary follicles. It is also present in secondary oocytes.1 Publication
Interactioni
Subunit structurei
Interacts with HSF2BP (via C-terminus).1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Hsf2bp | Q9D4G2 | 4 | EBI-8527667,EBI-8527688 |
Protein-protein interaction databases
IntActi | O35914, 1 interactor |
MINTi | O35914 |
STRINGi | 10090.ENSMUSP00000026096 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 219 | Hydrophobic | 10 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 501 – 507 | Nuclear localization signalBy similarity | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 826 – 843 | Ser-richAdd BLAST | 18 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 325 – 348 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 353 – 382 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 687 – 711 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 715 – 743 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 895 – 918 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 923 – 950 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG410IE3V Eukaryota ENOG410YN2D LUCA |
HOGENOMi | HOG000049098 |
HOVERGENi | HBG050706 |
InParanoidi | O35914 |
PhylomeDBi | O35914 |
Family and domain databases
InterProi | View protein in InterPro IPR040436 Disconnected-like IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type |
PANTHERi | PTHR15021 PTHR15021, 1 hit |
SMARTi | View protein in SMART SM00355 ZnF_C2H2, 6 hits |
SUPFAMi | SSF57667 SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 3 hits PS50157 ZINC_FINGER_C2H2_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O35914-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEAIGCTLN CSCQCFKPGK INHRQCEQCR HGWVAHALSK LRIPPVYPTS
60 70 80 90 100
QVEIVQSNVV FDISSLMLYG TQAIPVRLKI LLDRLFSVLK QDEVLQILHA
110 120 130 140 150
LDWTLQDYIR GYVLQDASGK VLDHWSIMTS EEEVATLQQF LRFGETKSIV
160 170 180 190 200
ELMAIQEKEE QSVIVPPTTA NVDIRAFIES CGHRSASLPT PVDKGSPGGM
210 220 230 240 250
HPFENLISNM TFMLPFQFFN PLPPALIGSL PEQYMLEQGQ DQSQEPKQEL
260 270 280 290 300
HGPFSDSSFL TSTPFQVEKE QCLNCPETVP QKEDSAHLSD SSSYSIASKL
310 320 330 340 350
ERTQLSPEAK VKPERNSLSA KKGRVFCTAC EKTFYDKGTL KIHYNAVHLK
360 370 380 390 400
IKHKCTIEGC NMVFSSLRSR NRHSANPNPR LHMPMNRNNR DKDLRNSLNL
410 420 430 440 450
ASSETYKRPG FTVVSPDCGP LPGYTGSVED SKGQPAFSSI GQNGVLFPNL
460 470 480 490 500
KTVQPVLPFY RSPATPAELA NTPGMLPSLP LLSSSIPEQL VSTDMPFDAL
510 520 530 540 550
PKKKSRKSSM PIKIEKEAVE IAEEKRHSLS SDDEVPLQVV SEDEPEDSSP
560 570 580 590 600
RSDRVPEEQH TQLSLEEPLP QGERACHLES VIESHGALSR TLEQTTLTER
610 620 630 640 650
EAEQKVALSS VMPREVEDGG HERHFTAGLV PQIPFPDYME LQQRLLAGGL
660 670 680 690 700
FGALSNRGMA FPFLEESKEL EHLGEHALVR QKEEARFQCD ICKKTFKNAC
710 720 730 740 750
SMKTHEKNTH ARETHACTVE GCGAAFPSRR SRDRHSSNLS LHQKVLNEEA
760 770 780 790 800
LETSEDHFRA AYLLQDVAKE AYQDVAFTPQ ASQTSVIFKG TSGMGSLVYP
810 820 830 840 850
ISQVHSASLE SYNSGPPSEG TILDLSTTSS MKSESSSHSS WDSDGVSEEG
860 870 880 890 900
TALMEDSDGN CEGQSLVSGE DEYPLCVLME KADQSLASLP SGLPITCHLC
910 920 930 940 950
QKIYSNKGTF RAHYKTVHLR QLHKCKVPGC NTMFSSVRSR NRHSQNPNLH
960
KSLASSPSHL Q
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8VPY0 | F8VPY0_MOUSE | Zinc finger protein basonuclin-1 | Bnc1 | 990 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | A → V in AAB94675 (PubMed:9687312).Curated | 1 | |
Sequence conflicti | 234 | Y → D in AAB94675 (PubMed:9687312).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88064 mRNA Translation: AAC53347.1 AF025301 mRNA Translation: AAB94675.1 |
UniGenei | Mm.243802 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88064 mRNA Translation: AAC53347.1 AF025301 mRNA Translation: AAB94675.1 |
UniGenei | Mm.243802 |
3D structure databases
ProteinModelPortali | O35914 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
IntActi | O35914, 1 interactor |
MINTi | O35914 |
STRINGi | 10090.ENSMUSP00000026096 |
PTM databases
iPTMneti | O35914 |
PhosphoSitePlusi | O35914 |
Proteomic databases
PaxDbi | O35914 |
PRIDEi | O35914 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Organism-specific databases
MGIi | MGI:1097164 Bnc1 |
Phylogenomic databases
eggNOGi | ENOG410IE3V Eukaryota ENOG410YN2D LUCA |
HOGENOMi | HOG000049098 |
HOVERGENi | HBG050706 |
InParanoidi | O35914 |
PhylomeDBi | O35914 |
Miscellaneous databases
PROi | PR:O35914 |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR040436 Disconnected-like IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type |
PANTHERi | PTHR15021 PTHR15021, 1 hit |
SMARTi | View protein in SMART SM00355 ZnF_C2H2, 6 hits |
SUPFAMi | SSF57667 SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 3 hits PS50157 ZINC_FINGER_C2H2_2, 2 hits |
ProtoNeti | Search... |
Entry informationi
Entry namei | BNC1_MOUSE | |
Accessioni | O35914Primary (citable) accession number: O35914 Secondary accession number(s): O54886 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot