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Protein

Transcription factor Spi-B

Gene

Spib

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi174 – 257ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Spi-B
Gene namesi
Name:Spib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:892986 Spib

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041371 – 267Transcription factor Spi-BAdd BLAST267

Proteomic databases

MaxQBiO35906
PaxDbiO35906
PRIDEiO35906

Expressioni

Tissue specificityi

Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation.1 Publication

Gene expression databases

BgeeiENSMUSG00000008193 Expressed in 71 organ(s), highest expression level in bone marrow
CleanExiMM_SPIB
ExpressionAtlasiO35906 baseline and differential
GenevisibleiO35906 MM

Interactioni

Subunit structurei

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035539

Structurei

3D structure databases

ProteinModelPortaliO35906
SMRiO35906
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 31TAD1 (Acidic)Add BLAST31
Regioni41 – 62TAD2Add BLAST22

Domaini

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes (By similarity).By similarity

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiO35906
KOiK09439
OMAiVQLCYGP
OrthoDBiEOG091G0DTY
PhylomeDBiO35906
TreeFamiTF352494

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform IA (identifier: O35906-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA
60 70 80 90 100
PPAPAIAPYE AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL
110 120 130 140 150
EAPGPGLQVY PPEDFTSQTL GSLAYAPYPS PVLSEEEDIM LDSPALEVSD
160 170 180 190 200
SESDEALLAG SEGRGSEAGA RKKLRLYQFL LGLLLRGDMR ECVWWVEPGA
210 220 230 240 250
GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA KTGEIRKVKR
260
KLTYQFDSAL LPASRHV
Note: Produced by alternative promoter usage.
Length:267
Mass (Da):29,365
Last modified:June 21, 2005 - v2
Checksum:iD32485A93C44327F
GO
Isoform IC (identifier: O35906-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-17: L → LVGEGVSPRPEMTLSSLRQ

Note: Produced by alternative splicing of isoform IA. No experimental confirmation available.
Show »
Length:285
Mass (Da):31,290
Checksum:iAA7AC02B7E1AACC9
GO
Isoform IIA (identifier: O35906-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MLALEAAQ → MPLCLSPAR

Note: Produced by alternative promoter usage.
Show »
Length:268
Mass (Da):29,506
Checksum:i0FBAD29580730610
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPG0A0A0U1RPG0_MOUSE
Transcription factor Spi-B
Spib
267Annotation score:
A0A0X1KG64A0A0X1KG64_MOUSE
Transcription factor Spi-B
Spib
284Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0139071 – 8MLALEAAQ → MPLCLSPAR in isoform IIA. Curated8
Alternative sequenceiVSP_01390817L → LVGEGVSPRPEMTLSSLRQ in isoform IC. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87619
, U87614, U87616, U87617, U87618 Genomic DNA Translation: AAC53554.1
U87619
, U87614, U87616, U87617, U87618 Genomic DNA Translation: AAC53556.1
U87619
, U87616, U87617, U87618 Genomic DNA Translation: AAC53557.1
U87620 Genomic DNA Translation: AAC53559.1
CCDSiCCDS21209.1 [O35906-1]
RefSeqiNP_063919.1, NM_019866.1 [O35906-1]
XP_006540996.1, XM_006540933.3 [O35906-2]
XP_006540998.1, XM_006540935.3 [O35906-3]
UniGeneiMm.8012

Genome annotation databases

EnsembliENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193 [O35906-1]
GeneIDi272382
KEGGimmu:272382
UCSCiuc009gpw.1 mouse [O35906-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87619
, U87614, U87616, U87617, U87618 Genomic DNA Translation: AAC53554.1
U87619
, U87614, U87616, U87617, U87618 Genomic DNA Translation: AAC53556.1
U87619
, U87616, U87617, U87618 Genomic DNA Translation: AAC53557.1
U87620 Genomic DNA Translation: AAC53559.1
CCDSiCCDS21209.1 [O35906-1]
RefSeqiNP_063919.1, NM_019866.1 [O35906-1]
XP_006540996.1, XM_006540933.3 [O35906-2]
XP_006540998.1, XM_006540935.3 [O35906-3]
UniGeneiMm.8012

3D structure databases

ProteinModelPortaliO35906
SMRiO35906
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035539

Proteomic databases

MaxQBiO35906
PaxDbiO35906
PRIDEiO35906

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193 [O35906-1]
GeneIDi272382
KEGGimmu:272382
UCSCiuc009gpw.1 mouse [O35906-1]

Organism-specific databases

CTDi6689
MGIiMGI:892986 Spib

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiO35906
KOiK09439
OMAiVQLCYGP
OrthoDBiEOG091G0DTY
PhylomeDBiO35906
TreeFamiTF352494

Miscellaneous databases

PROiPR:O35906
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008193 Expressed in 71 organ(s), highest expression level in bone marrow
CleanExiMM_SPIB
ExpressionAtlasiO35906 baseline and differential
GenevisibleiO35906 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPIB_MOUSE
AccessioniPrimary (citable) accession number: O35906
Secondary accession number(s): O35907, O35909, O55199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: November 7, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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