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Entry version 147 (10 Apr 2019)
Sequence version 1 (01 Jan 1998)
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Protein

Afadin

Gene

Afdn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm.

Miscellaneous

Isoform 1 increases the viscosity of F-actin in a dose-dependent manner. Isoform 1 causes F-actin to associate into bundles and meshworks.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • cell adhesion molecule binding Source: GO_Central
  • LIM domain binding Source: RGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell adhesion

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Afadin
Alternative name(s):
Afadin adherens junction formation factorImported
Protein Af-6
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:AfdnImported
Synonyms:Af6, Mllt4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Rat genome database

More...
RGDi
708561 Afdn

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002159201 – 1829AfadinAdd BLAST1829

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei216PhosphoserineBy similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei256PhosphoserineBy similarity1
Modified residuei391PhosphoserineCombined sources1
Modified residuei424PhosphoserineBy similarity1
Modified residuei512PhosphoserineCombined sources1
Modified residuei557PhosphoserineBy similarity1
Modified residuei562PhosphoserineBy similarity1
Modified residuei589PhosphoserineBy similarity1
Modified residuei655PhosphoserineBy similarity1
Modified residuei1090PhosphoserineCombined sources1
Modified residuei1114PhosphoserineCombined sources1
Modified residuei1133PhosphoserineBy similarity1
Modified residuei1147PhosphoserineCombined sources1
Modified residuei1150PhosphoserineCombined sources1
Modified residuei1179PhosphoserineBy similarity1
Modified residuei1180PhosphoserineBy similarity1
Modified residuei1189PhosphoserineCombined sources1
Modified residuei1206PhosphoserineBy similarity1
Modified residuei1218PhosphothreonineBy similarity1
Modified residuei1239PhosphothreonineBy similarity1
Modified residuei1245PhosphoserineBy similarity1
Modified residuei1282PhosphoserineCombined sources1
Modified residuei1335PhosphoserineBy similarity1
Modified residuei1337PhosphothreonineBy similarity1
Modified residuei1506PhosphoserineCombined sources1
Modified residuei1517PhosphoserineCombined sources1
Modified residuei1701PhosphoserineBy similarity1
Modified residuei1726PhosphoserineCombined sources1
Modified residuei1779PhosphoserineCombined sources1
Modified residuei1804PhosphoserineCombined sources1
Modified residuei1812N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
O35889

PRoteomics IDEntifications database

More...
PRIDEi
O35889

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O35889

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
O35889

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Isoform 1 is widely expressed, including in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is mainly expressed in the brain.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer. Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 2/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1, RIT2, NRXN1 and BCR (By similarity).By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
247803, 1 interactor

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...
ELMi
O35889

Protein interaction database and analysis system

More...
IntActi
O35889, 12 interactors

Molecular INTeraction database

More...
MINTi
O35889

STRING: functional protein association networks

More...
STRINGi
10116.ENSRNOP00000029044

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

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ProteinModelPortali
O35889

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O35889

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini39 – 133Ras-associating 1PROSITE-ProRule annotationAdd BLAST95
Domaini246 – 348Ras-associating 2PROSITE-ProRule annotationAdd BLAST103
Domaini441 – 507FHAAdd BLAST67
Domaini668 – 915DilutePROSITE-ProRule annotationAdd BLAST248
Domaini1014 – 1100PDZPROSITE-ProRule annotationAdd BLAST87

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili146 – 186Sequence analysisAdd BLAST41
Coiled coili1417 – 1454Sequence analysisAdd BLAST38
Coiled coili1530 – 1564Sequence analysisAdd BLAST35
Coiled coili1600 – 1672Sequence analysisAdd BLAST73

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1353 – 1399Pro-richAdd BLAST47
Compositional biasi1683 – 1713Pro-richAdd BLAST31
Compositional biasi1739 – 1742Poly-Asp4

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000008041

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG050463

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
O35889

KEGG Orthology (KO)

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KOi
K05702

Database of Orthologous Groups

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OrthoDBi
23029at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O35889

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00060 FHA, 1 hit
cd15471 Myo5p-like_CBD_afadin, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR028842 Afadin
IPR037977 CBD_Afadin
IPR002710 Dilute_dom
IPR000253 FHA_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000159 RA_dom
IPR008984 SMAD_FHA_dom_sf
IPR029071 Ubiquitin-like_domsf

The PANTHER Classification System

More...
PANTHERi
PTHR10398 PTHR10398, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01843 DIL, 1 hit
PF00498 FHA, 1 hit
PF00595 PDZ, 1 hit
PF00788 RA, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01132 DIL, 1 hit
SM00240 FHA, 1 hit
SM00228 PDZ, 1 hit
SM00314 RA, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49879 SSF49879, 1 hit
SSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51126 DILUTE, 1 hit
PS50106 PDZ, 1 hit
PS50200 RA, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O35889-1) [UniParc]FASTAAdd to basket
Also known as: l-afadin, p205

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK
60 70 80 90 100
AAGNFATKCI RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG
110 120 130 140 150
ERRLDIDEKP LVVQLNWNKD DREGRFVLKN ENDAIPAKKA QSNGPEKQEK
160 170 180 190 200
EGVIQNFKRT LSKKEKKEKK KREKEALRQA SDKEERPSQG DDSENSRLAA
210 220 230 240 250
EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS DGRPDSGGTL
260 270 280 290 300
RIYADSLKPN IPYKTILLST TDPADFAVAE SLEKYGLEKE NPKDYCIARV
310 320 330 340 350
MLPPGAQHSD ERGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDY
360 370 380 390 400
IPKKMKKHVE GKPLKGKDRA DGSGYGSALP PEKLPYLVEL SPGRRNHFAY
410 420 430 440 450
YSYHTYEDGS DSRDKPKLYR LQLSVTEVGT EKFDDNSIQL FGPGIQPHHC
460 470 480 490 500
DLTNMDGVVT VTPRSMDAET YVDGQRISET TMLQSGMRLQ FGTSHVFKFV
510 520 530 540 550
DPIQDHVLSK RSVDGGLMVK GPRHKPGAVQ ETTFELGGDI HSGTALPASR
560 570 580 590 600
STTRLDSDRV SSASSTAERG MVKPMIRLDQ EQDYRRRESR TQDAAGPELM
610 620 630 640 650
LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSSQH
660 670 680 690 700
RPDISPTERT HKAIAVVNKM VSMMEGVIQE VDQVDQKQKN IAGALAFWMA
710 720 730 740 750
NASELLNFIK QDRDLSRITL DAQDVLAHLV QMAFKYLVHC LQSELNNYMP
760 770 780 790 800
AFLDDPEENS LQRPKIDDVL HTLTGAMSLL RRCRVNAALT IQLFSQLFHF
810 820 830 840 850
INMWLFNRLV TDPDSGLCSH YWGAIIRQQL GHIEAWAEKQ GLELAADCHL
860 870 880 890 900
SRIVQATTLL TMDKYVPDDI PNINSTCFKL NSLQLQALLQ NYHCAPDEPF
910 920 930 940 950
IPTDLIENVV AVAENTADEL ARSDGRDVQL EEDPDLQLPF LLPEDGYSCD
960 970 980 990 1000
VVRNIPNGLQ EFLDPLCQRG FCRLVPHTRS PGTWTIYFEG ADYESHLMRE
1010 1020 1030 1040 1050
NTELTQPLRK EPEVITVTLK KQNGMGLSIV AAKGAGQDKL GIYVKSVVKG
1060 1070 1080 1090 1100
GAADVDGRLA AGDQLLSVDG RSLVGLSQER AAELMTRTSS VVTLEVAKQG
1110 1120 1130 1140 1150
AIYHGLATLL NQPSPMMQRI SDRRGSGKPR PKSEGFELYN NSAQNGSPES
1160 1170 1180 1190 1200
PQMPWTEYSE PKKLPGDDRL MKNRADHRSS PNVANQPPSP GGKSPYTSGT
1210 1220 1230 1240 1250
AAKITSVSTG NLCTEEQTPP PRPEAYPIPT QTYTREYFTF PASKSQDRMA
1260 1270 1280 1290 1300
PVQNQWPNYE EKPHMHTESD HASIAIQRVT RSQEELREEK VYQLERHRVE
1310 1320 1330 1340 1350
SGMDRKCDSD MWINQSSSVE SSTSSQEHLN HSSKSVTPAS TLTKSGPGRW
1360 1370 1380 1390 1400
KTPAAVLPTP VAVSQPIRTD LPPPPPPPPA HYTSDFDGIS MDLPLPPPPA
1410 1420 1430 1440 1450
NQAAPQSAQV AAAERKKREE HQRWYEKEKA RLEEERERKR REQERKLGQM
1460 1470 1480 1490 1500
RTQSLNPASF SPLATQAKPE KPSTLQRPQE TVIRELQPQQ QPRTIERRDL
1510 1520 1530 1540 1550
QYITISKEEL SSGDSLSPDP WKRDAREKLE KQQQMHIVDM LSKEIHELQN
1560 1570 1580 1590 1600
KGDRTAEESD RLRKLMLEWQ FQKRLQESKQ KDEDDDEEED DDVDTMLIMQ
1610 1620 1630 1640 1650
RLEAERRARL QDEERRRQQQ LEEMRKREVE DRVRQEEDGR HQEEERVKRD
1660 1670 1680 1690 1700
AEEKRRQEEG YYSRLEAERR RQHEEAARRL LEPEEPGLSR PPLPQDYEPP
1710 1720 1730 1740 1750
SQSSAPSAPP PPPQRNASYL KTQVLSPDSL FTAKFVAYDD DDEEENYVPA
1760 1770 1780 1790 1800
GPNSYSGSAG TTAGTYDAPR DTREKLSRSQ DADLPGSSGA PENLTFRERQ
1810 1820
RLFSQGQDVS DKVKASRKLT ELENELNTK
Length:1,829
Mass (Da):207,678
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i45C597A82F109D6F
GO
Isoform 2 (identifier: O35889-2) [UniParc]FASTAAdd to basket
Also known as: s-afadin, p190

The sequence of this isoform differs from the canonical sequence as follows:
     679-685: Missing.
     1609-1609: R → RQTAMPAISVLDL
     1655-1658: RRQE → VMVL
     1659-1829: Missing.

Show »
Length:1,663
Mass (Da):188,981
Checksum:i65C54B0ED2D0C74C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1LT10F1LT10_RAT
Afadin
Afdn
1,773Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_011726679 – 685Missing in isoform 2. 1 Publication7
Alternative sequenceiVSP_0117271609R → RQTAMPAISVLDL in isoform 2. 1 Publication1
Alternative sequenceiVSP_0117281655 – 1658RRQE → VMVL in isoform 2. 1 Publication4
Alternative sequenceiVSP_0117291659 – 1829Missing in isoform 2. 1 PublicationAdd BLAST171

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U83231 mRNA Translation: AAC53391.1
U83230 mRNA Translation: AAC53390.1

Protein sequence database of the Protein Information Resource

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PIRi
T41751
T42092

NCBI Reference Sequences

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RefSeqi
NP_037349.1, NM_013217.2 [O35889-1]

UniGene gene-oriented nucleotide sequence clusters

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UniGenei
Rn.58

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
26955

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
rno:26955

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83231 mRNA Translation: AAC53391.1
U83230 mRNA Translation: AAC53390.1
PIRiT41751
T42092
RefSeqiNP_037349.1, NM_013217.2 [O35889-1]
UniGeneiRn.58

3D structure databases

ProteinModelPortaliO35889
SMRiO35889
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247803, 1 interactor
ELMiO35889
IntActiO35889, 12 interactors
MINTiO35889
STRINGi10116.ENSRNOP00000029044

PTM databases

iPTMnetiO35889
PhosphoSitePlusiO35889

Proteomic databases

jPOSTiO35889
PRIDEiO35889

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi26955
KEGGirno:26955

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
4301
RGDi708561 Afdn

Phylogenomic databases

HOGENOMiHOG000008041
HOVERGENiHBG050463
InParanoidiO35889
KOiK05702
OrthoDBi23029at2759
PhylomeDBiO35889

Miscellaneous databases

Protein Ontology

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PROi
PR:O35889

Family and domain databases

CDDicd00060 FHA, 1 hit
cd15471 Myo5p-like_CBD_afadin, 1 hit
InterProiView protein in InterPro
IPR028842 Afadin
IPR037977 CBD_Afadin
IPR002710 Dilute_dom
IPR000253 FHA_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000159 RA_dom
IPR008984 SMAD_FHA_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10398 PTHR10398, 1 hit
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00498 FHA, 1 hit
PF00595 PDZ, 1 hit
PF00788 RA, 2 hits
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00240 FHA, 1 hit
SM00228 PDZ, 1 hit
SM00314 RA, 2 hits
SUPFAMiSSF49879 SSF49879, 1 hit
SSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50106 PDZ, 1 hit
PS50200 RA, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAFAD_RAT
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O35889
Secondary accession number(s): O35890
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 1, 1998
Last modified: April 10, 2019
This is version 147 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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