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Protein

COP9 signalosome complex subunit 5

Gene

Cops5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Promotes the proteasomal degradation of BRSK2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.By similarity

Miscellaneous

The CSN complex is associated with some 'Lys-63'-specific deubiquitination. Such activity is however not mediated by the core CSN complex but by the BRCC3/BRCC36 component of the BRISC complex (By similarity).By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi140Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi151Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5696394 DNA Damage Recognition in GG-NER
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8951664 Neddylation

Protein family/group databases

MEROPSiM67.002

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 5 (EC:3.4.-.-)
Short name:
SGN5
Short name:
Signalosome subunit 5
Alternative name(s):
Jun activation domain-binding protein 1
Kip1 C-terminus-interacting protein 2
Gene namesi
Name:Cops5
Synonyms:Csn5, Jab1, Kic2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1349415 Cops5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Nucleus, Signalosome, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151D → N: Abolishes ability to deneddylate cullins, but keeps ability to stabilize HIF1A protein. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001948362 – 334COP9 signalosome complex subunit 5Add BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO35864
MaxQBiO35864
PaxDbiO35864
PeptideAtlasiO35864
PRIDEiO35864

2D gel databases

REPRODUCTION-2DPAGEiO35864

PTM databases

iPTMnetiO35864
PhosphoSitePlusiO35864

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000025917 Expressed in 310 organ(s), highest expression level in testis
ExpressionAtlasiO35864 baseline and differential
GenevisibleiO35864 MM

Interactioni

Subunit structurei

Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B) and COPS9. The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with CDKN1B, HIF1A, ID1 and ID3. Interacts with IFIT3. Interacts with BRSK2 (By similarity). Interacts with ZDHHC16 (By similarity). Interacts with MINDY3 (By similarity). Interacts with FANK1; regulates the phosphorylation of JUN and the transcriptional activity of AP-1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205030, 20 interactors
CORUMiO35864
IntActiO35864, 2 interactors
MINTiO35864
STRINGi10090.ENSMUSP00000027050

Structurei

3D structure databases

ProteinModelPortaliO35864
SMRiO35864
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 192MPNPROSITE-ProRule annotationAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 151JAMM motifPROSITE-ProRule annotationAdd BLAST14

Domaini

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.

Sequence similaritiesi

Belongs to the peptidase M67A family. CSN5 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1554 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00550000074850
HOGENOMiHOG000116528
HOVERGENiHBG051137
InParanoidiO35864
KOiK09613
OMAiKYWVQTL
OrthoDBiEOG091G0AAO
PhylomeDBiO35864
TreeFamiTF105601

Family and domain databases

InterProiView protein in InterPro
IPR037740 CSN5
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
PANTHERiPTHR10410:SF6 PTHR10410:SF6, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

O35864-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASGSGMAQ KTWELANNMQ EAQSIDEIYK YDKKQQQEIL AAKPWTKDHH
60 70 80 90 100
YFKYCKISAL ALLKMVMHAR SGGNLEVMGL MLGKVDGETM IIMDSFALPV
110 120 130 140 150
EGTETRVNAQ AAAYEYMAAY IENAKQVGRL ENAIGWYHSH PGYGCWLSGI
160 170 180 190 200
DVSTQMLNQQ FQEPFVAVVI DPTRTISAGK VNLGAFRTYP KGYKPPDEGP
210 220 230 240 250
SEYQTIPLNK IEDFGVHCKQ YYALEVSYFK SSLDRKLLEL LWNKYWVNTL
260 270 280 290 300
SSSSLLTNAD YTTGQVFDLS EKLEQSEAQL GRGSFMLGLE THDRKSEDKL
310 320 330
AKATRDSCKT TIEAIHGLMS QVIKDKLFNQ INVA
Length:334
Mass (Da):37,549
Last modified:January 23, 2007 - v3
Checksum:iAD06BF4AAD03A1CF
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WQA8A0A087WQA8_MOUSE
COP9 signalosome complex subunit 5
Cops5
210Annotation score:
A0A087WQ60A0A087WQ60_MOUSE
COP9 signalosome complex subunit 5
Cops5
147Annotation score:
A0A087WRH6A0A087WRH6_MOUSE
COP9 signalosome complex subunit 5
Cops5
48Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70736 mRNA Translation: AAD03470.1
AF068223 mRNA Translation: AAF61318.1
AF065386 mRNA Translation: AAC17179.1
AK012499 mRNA Translation: BAB28282.1
AK146271 mRNA Translation: BAE27030.1
AK151492 mRNA Translation: BAE30445.1
BC046753 mRNA Translation: AAH46753.1
CCDSiCCDS14818.1
RefSeqiNP_001264030.1, NM_001277101.1
NP_038743.1, NM_013715.2
UniGeneiMm.402384

Genome annotation databases

EnsembliENSMUST00000027050; ENSMUSP00000027050; ENSMUSG00000025917
GeneIDi26754
KEGGimmu:26754
UCSCiuc007ahe.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCSN5_MOUSE
AccessioniPrimary (citable) accession number: O35864
Secondary accession number(s): Q3UA70, Q8C1S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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