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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

Timm44

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 173ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: MGI
  • protein import into mitochondrial matrix Source: GO_Central

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Gene namesi
Name:Timm44
Synonyms:Mimt44, Tim44
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1343262 Timm44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000034315? – 452Mitochondrial import inner membrane translocase subunit TIM44
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphothreonineBy similarity1
Modified residuei177N6-succinyllysineCombined sources1
Modified residuei180PhosphoserineBy similarity1
Modified residuei217N6-succinyllysineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35857
MaxQBiO35857
PaxDbiO35857
PeptideAtlasiO35857
PRIDEiO35857

2D gel databases

REPRODUCTION-2DPAGEiO35857

PTM databases

iPTMnetiO35857
PhosphoSitePlusiO35857
SwissPalmiO35857

Expressioni

Gene expression databases

BgeeiENSMUSG00000002949 Expressed in 290 organ(s), highest expression level in iris
CleanExiMM_TIMM44
GenevisibleiO35857 MM

Interactioni

Subunit structurei

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19. The complex interacts with the TIMM23 component of the TIM23 complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35857, 1 interactor
STRINGi10090.ENSMUSP00000003029

Structurei

3D structure databases

ProteinModelPortaliO35857
SMRiO35857
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2580 Eukaryota
ENOG410Y5IK LUCA
GeneTreeiENSGT00390000000051
HOGENOMiHOG000013004
HOVERGENiHBG055113
InParanoidiO35857
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG091G073E
TreeFamiTF106197

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
TIGRFAMsiTIGR00984 3a0801s03tim44, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35857-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAALRGGW CRCPRRCLGS GIQFLSSHNL PHGSSYQISR PGRELTLTKS
60 70 80 90 100
YSSGSRKGFL SGLLDNIKQE LAKNKEMKES IKKFRDEAKK LEESDALQEA
110 120 130 140 150
RRKYKSIESE TVRTSEAIKK KLGELTGTVK ESLDEVSKSD LGRKIKEGVE
160 170 180 190 200
EAARTAKQSA ESVSKSGEKL GKTAAFKAIS QGVESVKKEL DESVLGQTGP
210 220 230 240 250
YRRPERLRKR TEFAGAKFKE SKVFEANEEA LGVVLHKDSK WYQQWKDFKD
260 270 280 290 300
NNVVFNRFFE MKMKYDESDN VLIRASRALT DKVTDLLGGL FSKTEMSEVL
310 320 330 340 350
TEILRVDPTF DKDHFLHQCE TDIIPNILEA MISGELDILK DWCYEATYSQ
360 370 380 390 400
LAHPIQQAKA LGFQFHSRIL DISNVDLAMG KMMEQGPVLI VTFQAQVVMV
410 420 430 440 450
IKNSKGEVYD GDPDKVQRML YVWALCRDQE ELNPYAAWRL LDISASSTEQ

IL
Length:452
Mass (Da):51,091
Last modified:July 27, 2011 - v2
Checksum:iDC3E5F4E43972D2F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5A → R in AAB97624 (PubMed:9419343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69898 mRNA Translation: AAB97624.1
CH466566 Genomic DNA Translation: EDL21992.1
BC110677 mRNA Translation: AAI10678.1
BC117523 mRNA Translation: AAI17524.1
BC117524 mRNA Translation: AAI17525.1
CCDSiCCDS22083.1
RefSeqiNP_035722.2, NM_011592.2
UniGeneiMm.195249

Genome annotation databases

EnsembliENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949
GeneIDi21856
KEGGimmu:21856
UCSCiuc009ktq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69898 mRNA Translation: AAB97624.1
CH466566 Genomic DNA Translation: EDL21992.1
BC110677 mRNA Translation: AAI10678.1
BC117523 mRNA Translation: AAI17524.1
BC117524 mRNA Translation: AAI17525.1
CCDSiCCDS22083.1
RefSeqiNP_035722.2, NM_011592.2
UniGeneiMm.195249

3D structure databases

ProteinModelPortaliO35857
SMRiO35857
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35857, 1 interactor
STRINGi10090.ENSMUSP00000003029

PTM databases

iPTMnetiO35857
PhosphoSitePlusiO35857
SwissPalmiO35857

2D gel databases

REPRODUCTION-2DPAGEiO35857

Proteomic databases

EPDiO35857
MaxQBiO35857
PaxDbiO35857
PeptideAtlasiO35857
PRIDEiO35857

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949
GeneIDi21856
KEGGimmu:21856
UCSCiuc009ktq.2 mouse

Organism-specific databases

CTDi10469
MGIiMGI:1343262 Timm44

Phylogenomic databases

eggNOGiKOG2580 Eukaryota
ENOG410Y5IK LUCA
GeneTreeiENSGT00390000000051
HOGENOMiHOG000013004
HOVERGENiHBG055113
InParanoidiO35857
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG091G073E
TreeFamiTF106197

Miscellaneous databases

ChiTaRSiTimm44 mouse
PROiPR:O35857
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002949 Expressed in 290 organ(s), highest expression level in iris
CleanExiMM_TIMM44
GenevisibleiO35857 MM

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
TIGRFAMsiTIGR00984 3a0801s03tim44, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTIM44_MOUSE
AccessioniPrimary (citable) accession number: O35857
Secondary accession number(s): Q2NLC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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