UniProtKB - O35855 (BCAT2_MOUSE)
Protein
Branched-chain-amino-acid aminotransferase, mitochondrial
Gene
Bcat2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
Catalytic activityi
Cofactori
GO - Molecular functioni
- branched-chain-amino-acid transaminase activity Source: MGI
- L-isoleucine transaminase activity Source: UniProtKB-EC
- L-leucine transaminase activity Source: UniProtKB-EC
- L-valine transaminase activity Source: UniProtKB-EC
GO - Biological processi
- branched-chain amino acid catabolic process Source: MGI
- branched-chain amino acid metabolic process Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- isoleucine catabolic process Source: MGI
- isoleucine metabolic process Source: MGI
- leucine biosynthetic process Source: MGI
- leucine metabolic process Source: MGI
- regulation of hormone levels Source: MGI
- valine biosynthetic process Source: GO_Central
- valine metabolic process Source: MGI
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-MMU-70895, Branched-chain amino acid catabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)Short name: BCAT(m) |
Gene namesi | Name:Bcat2 Synonyms:Bcatm, Eca40 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1276534, Bcat2 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 27 | MitochondrionBy similarityAdd BLAST | 27 | |
ChainiPRO_0000001272 | 28 – 393 | Branched-chain-amino-acid aminotransferase, mitochondrialAdd BLAST | 366 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 230 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 322 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | O35855 |
jPOSTi | O35855 |
PaxDbi | O35855 |
PeptideAtlasi | O35855 |
PRIDEi | O35855 |
2D gel databases
REPRODUCTION-2DPAGEi | O35855 |
PTM databases
iPTMneti | O35855 |
PhosphoSitePlusi | O35855 |
SwissPalmi | O35855 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030826, Expressed in stomach and 108 other tissues |
Genevisiblei | O35855, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
IntActi | O35855, 2 interactors |
MINTi | O35855 |
STRINGi | 10090.ENSMUSP00000033098 |
Miscellaneous databases
RNActi | O35855, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
InParanoidi | O35855 |
OrthoDBi | 853728at2759 |
PhylomeDBi | O35855 |
TreeFami | TF300882 |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O35855-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAATLGQVW ARKLLPVPWL LCGSKRCVSS IFKAADLQIQ MTKEPQKKPA
60 70 80 90 100
PSQALLFGKT FTDHMLMVEW NNKAGWGPPR IQPFQNLTLH PACSGLHYSL
110 120 130 140 150
QLFEGLKAYK GGDQQVRLFR PWLNMDRMLR SARRLCLPDF DKQELLECIR
160 170 180 190 200
QLIEVDKDWV PDGNGTSLYV RPVLIGNEPS LGVGMVTQAL LYVILCPVGS
210 220 230 240 250
YFPGDSMTPV SLLADPSFVR AWIGGVGDCK LGGNYGPTVA VQREAQKRGC
260 270 280 290 300
EQVLWLYGPD HQLTEVGTMN IFVYWTHEDG VLELVTPPLN GVILPGVVRQ
310 320 330 340 350
SLLDLARTWG EFRVAERKVT MKELKRALEE GRVREVFGSG TACQVCPVHQ
360 370 380 390
ILYEGKQLHI PTMENGPELI LRFQKELKAI QYGASAHDWM FRV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketO88374 | O88374_MOUSE | Branched-chain-amino-acid aminotran... | Bcat2 Eca40 | 353 | Annotation score: | ||
A0A1B0GX27 | A0A1B0GX27_MOUSE | Branched-chain-amino-acid aminotran... | Bcat2 | 388 | Annotation score: | ||
A0A1B0GQY4 | A0A1B0GQY4_MOUSE | Branched-chain-amino-acid aminotran... | Bcat2 | 101 | Annotation score: | ||
A0A1B0GST1 | A0A1B0GST1_MOUSE | Branched-chain-amino-acid aminotran... | Bcat2 | 97 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 199 | G → A in AAB67674 (PubMed:9165094).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC017688 mRNA Translation: AAH17688.1 BC048072 mRNA Translation: AAH48072.1 U68526 mRNA Translation: AAB67674.1 |
CCDSi | CCDS21252.1 |
RefSeqi | NP_033867.1, NM_009737.3 |
Genome annotation databases
Ensembli | ENSMUST00000033098; ENSMUSP00000033098; ENSMUSG00000030826 |
GeneIDi | 12036 |
KEGGi | mmu:12036 |
UCSCi | uc009gwc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC017688 mRNA Translation: AAH17688.1 BC048072 mRNA Translation: AAH48072.1 U68526 mRNA Translation: AAB67674.1 |
CCDSi | CCDS21252.1 |
RefSeqi | NP_033867.1, NM_009737.3 |
3D structure databases
SMRi | O35855 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O35855, 2 interactors |
MINTi | O35855 |
STRINGi | 10090.ENSMUSP00000033098 |
PTM databases
iPTMneti | O35855 |
PhosphoSitePlusi | O35855 |
SwissPalmi | O35855 |
2D gel databases
REPRODUCTION-2DPAGEi | O35855 |
Proteomic databases
EPDi | O35855 |
jPOSTi | O35855 |
PaxDbi | O35855 |
PeptideAtlasi | O35855 |
PRIDEi | O35855 |
Protocols and materials databases
Antibodypediai | 31810, 251 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033098; ENSMUSP00000033098; ENSMUSG00000030826 |
GeneIDi | 12036 |
KEGGi | mmu:12036 |
UCSCi | uc009gwc.2, mouse |
Organism-specific databases
CTDi | 587 |
MGIi | MGI:1276534, Bcat2 |
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
InParanoidi | O35855 |
OrthoDBi | 853728at2759 |
PhylomeDBi | O35855 |
TreeFami | TF300882 |
Enzyme and pathway databases
Reactomei | R-MMU-70895, Branched-chain amino acid catabolism |
Miscellaneous databases
BioGRID-ORCSi | 12036, 1 hit in 18 CRISPR screens |
ChiTaRSi | Bcat2, mouse |
PROi | PR:O35855 |
RNActi | O35855, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030826, Expressed in stomach and 108 other tissues |
Genevisiblei | O35855, MM |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAT2_MOUSE | |
Accessioni | O35855Primary (citable) accession number: O35855 Secondary accession number(s): Q8VD03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families