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UniProtKB - O35738 (KLF12_MOUSE)
Protein
Krueppel-like factor 12
Gene
Klf12
Organism
Mus musculus (Mouse)
Status
Functioni
Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 317 – 341 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 347 – 371 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 377 – 399 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 12Alternative name(s): Transcriptional repressor AP-2rep |
Gene namesi | Name:Klf12 Synonyms:Ap2rep |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333796, Klf12 |
VEuPathDBi | HostDB:ENSMUSG00000072294 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047183 | 1 – 402 | Krueppel-like factor 12Add BLAST | 402 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 313 | N6-methylated lysine; by EHMT2By similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
MaxQBi | O35738 |
PaxDbi | O35738 |
PeptideAtlasi | O35738 |
PRIDEi | O35738 |
ProteomicsDBi | 263618 |
PTM databases
iPTMneti | O35738 |
PhosphoSitePlusi | O35738 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000072294, Expressed in head and 104 other tissues |
ExpressionAtlasi | O35738, baseline and differential |
Genevisiblei | O35738, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 200963, 9 interactors |
STRINGi | 10090.ENSMUSP00000094844 |
Miscellaneous databases
RNActi | O35738, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 144 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 219 – 254 | DisorderedSequence analysisAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 80 – 88 | 9aaTAD; inactiveBy similarity | 9 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF12, the motif is inactive.By similarity
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 317 – 341 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 347 – 371 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 377 – 399 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158108 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | O35738 |
OMAi | NIPMKRK |
OrthoDBi | 845652at2759 |
PhylomeDBi | O35738 |
TreeFami | TF350556 |
Family and domain databases
InterProi | View protein in InterPro IPR030447, KLF12 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF56, PTHR23235:SF56, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O35738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNIHMKRKTI KNLSALENRM LMLDGMPAVR VKTELVESEQ GSPNVHNYPD
60 70 80 90 100
MEAVPLLLNN VKGEPPEDSL PVDHFQTQTE PVDLSINKAR TSPTAASSSP
110 120 130 140 150
VSMTASASSP SSTSTSSSSS SRPASSPTVI TSVSSASSSS TVLSPGPLVA
160 170 180 190 200
SASGVGGQQF LHIIHPVPPS SPMNLQSNKL SHVHRIPVVV QSVPVVYTAV
210 220 230 240 250
RSPGNVNNTI VVPLLEDGRS HGKAQMEPRG LSPRQSKSDS DDDDLPNVTL
260 270 280 290 300
DSVNETGSTA LSIARAVQEV HPSPVSRVRG NRMNNQKFAC SISPFSIEST
310 320 330 340 350
RRQRRSESPD SRKRRIHRCD FEGCNKVYTK SSHLKAHRRT HTGEKPYKCT
360 370 380 390 400
WEGCTWKFAR SDELTRHYRK HTGVKPFKCA DCDRSFSRSD HLALHRRRHM
LV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BQ01 | A0A2I3BQ01_MOUSE | Krueppel-like factor 12 | Klf12 | 153 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 105 | A → G in CAA74670 (PubMed:9858544).Curated | 1 | |
Sequence conflicti | 135 | S → R in CAA74670 (PubMed:9858544).Curated | 1 | |
Sequence conflicti | 359 | A → G in CAA74670 (PubMed:9858544).Curated | 1 | |
Sequence conflicti | 385 | S → R in CAA74670 (PubMed:9858544).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14295 mRNA Translation: CAA74670.1 BC067408 mRNA Translation: AAH67408.1 |
CCDSi | CCDS36998.1 |
RefSeqi | NP_034766.2, NM_010636.3 XP_006518686.1, XM_006518623.3 XP_006518687.1, XM_006518624.3 XP_011243270.1, XM_011244968.2 XP_011243271.1, XM_011244969.2 |
Genome annotation databases
Ensembli | ENSMUST00000097079; ENSMUSP00000094844; ENSMUSG00000072294 ENSMUST00000228216; ENSMUSP00000153901; ENSMUSG00000072294 |
GeneIDi | 16597 |
KEGGi | mmu:16597 |
UCSCi | uc007uvh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14295 mRNA Translation: CAA74670.1 BC067408 mRNA Translation: AAH67408.1 |
CCDSi | CCDS36998.1 |
RefSeqi | NP_034766.2, NM_010636.3 XP_006518686.1, XM_006518623.3 XP_006518687.1, XM_006518624.3 XP_011243270.1, XM_011244968.2 XP_011243271.1, XM_011244969.2 |
3D structure databases
SMRi | O35738 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200963, 9 interactors |
STRINGi | 10090.ENSMUSP00000094844 |
PTM databases
iPTMneti | O35738 |
PhosphoSitePlusi | O35738 |
Proteomic databases
MaxQBi | O35738 |
PaxDbi | O35738 |
PeptideAtlasi | O35738 |
PRIDEi | O35738 |
ProteomicsDBi | 263618 |
Protocols and materials databases
Antibodypediai | 24434, 276 antibodies from 29 providers |
DNASUi | 16597 |
Genome annotation databases
Ensembli | ENSMUST00000097079; ENSMUSP00000094844; ENSMUSG00000072294 ENSMUST00000228216; ENSMUSP00000153901; ENSMUSG00000072294 |
GeneIDi | 16597 |
KEGGi | mmu:16597 |
UCSCi | uc007uvh.2, mouse |
Organism-specific databases
CTDi | 11278 |
MGIi | MGI:1333796, Klf12 |
VEuPathDBi | HostDB:ENSMUSG00000072294 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158108 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | O35738 |
OMAi | NIPMKRK |
OrthoDBi | 845652at2759 |
PhylomeDBi | O35738 |
TreeFami | TF350556 |
Miscellaneous databases
BioGRID-ORCSi | 16597, 1 hit in 63 CRISPR screens |
ChiTaRSi | Klf12, mouse |
PROi | PR:O35738 |
RNActi | O35738, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000072294, Expressed in head and 104 other tissues |
ExpressionAtlasi | O35738, baseline and differential |
Genevisiblei | O35738, MM |
Family and domain databases
InterProi | View protein in InterPro IPR030447, KLF12 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF56, PTHR23235:SF56, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF12_MOUSE | |
Accessioni | O35738Primary (citable) accession number: O35738 Secondary accession number(s): Q6NWV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families