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Protein

DNA repair protein RAD51 homolog 2

Gene

Rad51b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 2
Short name:
R51H2
Alternative name(s):
RAD51 homolog B
RAD51-like protein 1
Gene namesi
Name:Rad51b
Synonyms:Rad51l1, Rec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1099436 Rad51b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229401 – 350DNA repair protein RAD51 homolog 2Add BLAST350

Proteomic databases

MaxQBiO35719
PaxDbiO35719
PeptideAtlasiO35719
PRIDEiO35719

PTM databases

PhosphoSitePlusiO35719

Expressioni

Tissue specificityi

Expressed in a wide range of tissues.

Gene expression databases

BgeeiENSMUSG00000059060 Expressed in 31 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_RAD51L1
GenevisibleiO35719 MM

Interactioni

Subunit structurei

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair (By similarity).By similarity

Protein-protein interaction databases

BioGridi202566, 1 interactor
STRINGi10090.ENSMUSP00000078490

Structurei

3D structure databases

ProteinModelPortaliO35719
SMRiO35719
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 75Interaction with RAD51CBy similarityAdd BLAST75

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
GeneTreeiENSGT00860000133731
HOGENOMiHOG000154062
HOVERGENiHBG061476
InParanoidiO35719
KOiK10869
OMAiMQTAYEI
PhylomeDBiO35719
TreeFamiTF101219

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR030548 RAD51B
IPR020588 RecA_ATP-bd
PANTHERiPTHR22942:SF15 PTHR22942:SF15, 1 hit
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O35719-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSKKLRRVG LSPELCDRLS RYQIVNCQHF LSLSPLELMK VTGLSYRGVH
60 70 80 90 100
ELLHTVSKAC APQMQTAYEL KTRRSAHLSP AFLSTTLCAL DEALHGGVPC
110 120 130 140 150
GSLTEITGPP GCGKTQFCIM MSVLATLPTS LGGLEGAVVY IDTESAFTAE
160 170 180 190 200
RLVEIAESRF PQYFNTEEKL LLTSSRVHLC RELTCEGLLQ RLESLEEEII
210 220 230 240 250
SKGVKLVIVD SIASVVRKEF DPKLQGNIKE RNKFLGKGAS LLKYLAGEFS
260 270 280 290 300
IPVILTNQIT THLSGALPSQ ADLVSPADDL SLSEGTSGSS CLVAALGNTW
310 320 330 340 350
GHCVNTRLIL QYLDSERRQI LIAKSPLAAF TSFVYTIKGE GLVLQGHERP
Length:350
Mass (Da):38,152
Last modified:November 7, 2003 - v2
Checksum:i2DB79F8D60C0529D
GO
Isoform 2 (identifier: O35719-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-258: ILTNQ → TFQPI
     259-350: Missing.

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):28,421
Checksum:i94C2067795B15B37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23Q → L in AAB63359 (PubMed:9207106).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008869254 – 258ILTNQ → TFQPI in isoform 2. 1 Publication5
Alternative sequenceiVSP_008870259 – 350Missing in isoform 2. 1 PublicationAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92068 mRNA Translation: AAB63359.1
AK015771 mRNA Translation: BAB29970.1
AK032913 mRNA Translation: BAC28084.1
CCDSiCCDS56845.1 [O35719-2]
RefSeqiNP_001239491.1, NM_001252562.1 [O35719-2]
NP_033040.2, NM_009014.3 [O35719-1]
XP_011242347.1, XM_011244045.1 [O35719-2]
UniGeneiMm.341756

Genome annotation databases

EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060 [O35719-2]
ENSMUST00000171210; ENSMUSP00000128357; ENSMUSG00000059060 [O35719-1]
ENSMUST00000218039; ENSMUSP00000152105; ENSMUSG00000059060 [O35719-1]
GeneIDi19363
KEGGimmu:19363
UCSCiuc007oai.1 mouse [O35719-1]
uc007oaj.1 mouse [O35719-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92068 mRNA Translation: AAB63359.1
AK015771 mRNA Translation: BAB29970.1
AK032913 mRNA Translation: BAC28084.1
CCDSiCCDS56845.1 [O35719-2]
RefSeqiNP_001239491.1, NM_001252562.1 [O35719-2]
NP_033040.2, NM_009014.3 [O35719-1]
XP_011242347.1, XM_011244045.1 [O35719-2]
UniGeneiMm.341756

3D structure databases

ProteinModelPortaliO35719
SMRiO35719
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202566, 1 interactor
STRINGi10090.ENSMUSP00000078490

PTM databases

PhosphoSitePlusiO35719

Proteomic databases

MaxQBiO35719
PaxDbiO35719
PeptideAtlasiO35719
PRIDEiO35719

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060 [O35719-2]
ENSMUST00000171210; ENSMUSP00000128357; ENSMUSG00000059060 [O35719-1]
ENSMUST00000218039; ENSMUSP00000152105; ENSMUSG00000059060 [O35719-1]
GeneIDi19363
KEGGimmu:19363
UCSCiuc007oai.1 mouse [O35719-1]
uc007oaj.1 mouse [O35719-2]

Organism-specific databases

CTDi5890
MGIiMGI:1099436 Rad51b

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
GeneTreeiENSGT00860000133731
HOGENOMiHOG000154062
HOVERGENiHBG061476
InParanoidiO35719
KOiK10869
OMAiMQTAYEI
PhylomeDBiO35719
TreeFamiTF101219

Enzyme and pathway databases

ReactomeiR-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiRad51b mouse
PROiPR:O35719
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059060 Expressed in 31 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_RAD51L1
GenevisibleiO35719 MM

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR030548 RAD51B
IPR020588 RecA_ATP-bd
PANTHERiPTHR22942:SF15 PTHR22942:SF15, 1 hit
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRA51B_MOUSE
AccessioniPrimary (citable) accession number: O35719
Secondary accession number(s): Q8BSH6, Q9D555
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 7, 2003
Last modified: November 7, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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