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Protein

Nocturnin

Gene

Noct

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Circadian deadenylase which plays an important role in post-transcriptional regulation of metabolic genes under circadian control. Degrades poly(A) tails of specific target mRNAs leading to their degradation and suppression of translation. Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis. Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance. Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation. Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone. Critical for proper development of early embryos.8 Publications

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei193By similarity1
Metal bindingi193Magnesium 1By similarity1
Metal bindingi322Magnesium 2By similarity1
Metal bindingi324Magnesium 2By similarity1
Active sitei375By similarity1
Active sitei412By similarity1
Metal bindingi412Magnesium 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA binding Source: UniProtKB
  • poly(A)-specific ribonuclease activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding
Biological processBiological rhythms, mRNA processing
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.13.4 3474

Names & Taxonomyi

Protein namesi
Recommended name:
NocturninBy similarityImported (EC:3.1.13.4)
Alternative name(s):
Carbon catabolite repression 4-like protein
Circadian deadenylase NOC
Gene namesi
Name:NoctImported
Synonyms:Ccr4, Ccrn4l, Noc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109382 Noct

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit metabolic defects including a resistance to diet-induced obesity, decreased fat storage, changes in lipid-related gene expression profiles in the liver, and altered glucose and insulin sensitivities. Exhibit a delayed early embryo development and at 12 weeks of age show enhanced skeletal mass and increased osteoblastogenesis.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185691 – 429NocturninAdd BLAST429

Proteomic databases

MaxQBiO35710
PaxDbiO35710
PRIDEiO35710

PTM databases

iPTMnetiO35710
PhosphoSitePlusiO35710

Expressioni

Tissue specificityi

Highly expressed in the differentiated adipocyte (at protein level). Ubiquitous.3 Publications

Developmental stagei

Expression is highest in oocytes, begins to decrease after fertilization by the 4-cell stage and then slightly increases up to the blastocyst stage.1 Publication

Inductioni

Immediate early gene (IEG) showing acute responses to several stimuli including serum shock, phorbol ester, lipopolysaccharide (LPS) and rosiglitazone, a PPARG agonist. Exhibits a high amplitude circadian rhythm with maximal levels in early evening. In constant darkness or constant light, the amplitude of the rhythm decreases. Expression is regulated by both light and food-entrained cues and by the CLOCK-ARNTL/BMAL1 heterodimer and PPARG. Up-regulated in cells undergoing adipogenesis.4 Publications

Gene expression databases

BgeeiENSMUSG00000023087 Expressed in 291 organ(s), highest expression level in secondary oocyte
CleanExiMM_CCR4
ExpressionAtlasiO35710 baseline and differential
GenevisibleiO35710 MM

Interactioni

Subunit structurei

Interacts with PPARG.1 Publication

Protein-protein interaction databases

DIPiDIP-60436N
IntActiO35710, 1 interactor
STRINGi10090.ENSMUSP00000023849

Structurei

3D structure databases

ProteinModelPortaliO35710
SMRiO35710
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 351Interaction with PPARG1 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000237357
HOVERGENiHBG006512
InParanoidiO35710
KOiK18764
OMAiRTDCPSS
OrthoDBiEOG091G0BF8
PhylomeDBiO35710
TreeFamiTF323175

Family and domain databases

CDDicd09096 Deadenylase_nocturnin, 1 hit
Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR034965 Deadenylase_nocturnin
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O35710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYQSPRRLCS ALLLRDAPGL RRTLVPGPRR TLAPPVLGSR PKSPQLQAAA
60 70 80 90 100
ASGAARSRPR TVSSMGNGTS RLYSALAKTV NSSAAAQHPE YLVSTDPEHL
110 120 130 140 150
EPIDPKELLE ECRAVLHTRP PRYQRDFVDL RTDCSSSHSP IRVMQWNILA
160 170 180 190 200
QALGEGKDNF VQCPVEALKW EERKCLILEE ILAYQPDILC LQEVDHYFDT
210 220 230 240 250
FQPLLSRLGY QGTFFPKPWS PCLDVEHNNG PDGCALFFLQ NRFKLISSTN
260 270 280 290 300
IRLTAMTLKT NQVAIAQTLE CKESGRQFCI AVTHLKARTG WERFRSAQGC
310 320 330 340 350
DLLQNLQNIT QGAKIPLIVC GDFNAEPTEE VYKHFASSSL NLNSAYKLLS
360 370 380 390 400
PDGQSEPPYT TWKIRTSGEC RHTLDYIWYS RHALSVTSAL DLLTEEQIGP
410 420
NRLPSFHYPS DHLSLVCDFS FNEEPHELF
Length:429
Mass (Da):48,301
Last modified:November 2, 2001 - v3
Checksum:iCB9FB55D84E13942
GO
Isoform 2 (identifier: O35710-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: Missing.

Note: No experimental confirmation available.
Show »
Length:365
Mass (Da):41,500
Checksum:i762CA86E0F907EB8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YY01A0A0A6YY01_MOUSE
Nocturnin
Noct Ccrn4l
117Annotation score:

Sequence cautioni

The sequence AAB62717 differs from that shown. Reason: Frameshift at position 125.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0166771 – 64Missing in isoform 2. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70139 mRNA Translation: AAB62717.1 Frameshift.
AF183960 mRNA Translation: AAD56547.1
AF199491 mRNA Translation: AAG01384.1
AK087790 mRNA Translation: BAC40004.1
CCDSiCCDS17336.1 [O35710-1]
RefSeqiNP_033964.1, NM_009834.2 [O35710-1]
UniGeneiMm.86541

Genome annotation databases

EnsembliENSMUST00000023849; ENSMUSP00000023849; ENSMUSG00000023087 [O35710-1]
ENSMUST00000144826; ENSMUSP00000141416; ENSMUSG00000023087 [O35710-2]
ENSMUST00000167780; ENSMUSP00000130347; ENSMUSG00000023087 [O35710-1]
GeneIDi12457
KEGGimmu:12457
UCSCiuc008pdm.2 mouse [O35710-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70139 mRNA Translation: AAB62717.1 Frameshift.
AF183960 mRNA Translation: AAD56547.1
AF199491 mRNA Translation: AAG01384.1
AK087790 mRNA Translation: BAC40004.1
CCDSiCCDS17336.1 [O35710-1]
RefSeqiNP_033964.1, NM_009834.2 [O35710-1]
UniGeneiMm.86541

3D structure databases

ProteinModelPortaliO35710
SMRiO35710
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60436N
IntActiO35710, 1 interactor
STRINGi10090.ENSMUSP00000023849

PTM databases

iPTMnetiO35710
PhosphoSitePlusiO35710

Proteomic databases

MaxQBiO35710
PaxDbiO35710
PRIDEiO35710

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023849; ENSMUSP00000023849; ENSMUSG00000023087 [O35710-1]
ENSMUST00000144826; ENSMUSP00000141416; ENSMUSG00000023087 [O35710-2]
ENSMUST00000167780; ENSMUSP00000130347; ENSMUSG00000023087 [O35710-1]
GeneIDi12457
KEGGimmu:12457
UCSCiuc008pdm.2 mouse [O35710-1]

Organism-specific databases

CTDi25819
MGIiMGI:109382 Noct

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000237357
HOVERGENiHBG006512
InParanoidiO35710
KOiK18764
OMAiRTDCPSS
OrthoDBiEOG091G0BF8
PhylomeDBiO35710
TreeFamiTF323175

Enzyme and pathway databases

BRENDAi3.1.13.4 3474

Miscellaneous databases

ChiTaRSiNoct mouse
PROiPR:O35710
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023087 Expressed in 291 organ(s), highest expression level in secondary oocyte
CleanExiMM_CCR4
ExpressionAtlasiO35710 baseline and differential
GenevisibleiO35710 MM

Family and domain databases

CDDicd09096 Deadenylase_nocturnin, 1 hit
Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR034965 Deadenylase_nocturnin
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOCT_MOUSE
AccessioniPrimary (citable) accession number: O35710
Secondary accession number(s): Q9QZG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 2, 2001
Last modified: September 12, 2018
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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