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Protein

Serine palmitoyltransferase 1

Gene

Sptlc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis
UniPathwayi
UPA00222

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 1 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 1
Short name:
LCB 1
Serine-palmitoyl-CoA transferase 1
Short name:
SPT 1
Short name:
SPT1
Gene namesi
Name:Sptlc1
Synonyms:Lcb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1099431 Sptlc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15LumenalSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 473CytoplasmicSequence analysisAdd BLAST437

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi2509

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638551 – 473Serine palmitoyltransferase 1Add BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164Phosphotyrosine; by ABLBy similarity1

Post-translational modificationi

Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35704
MaxQBiO35704
PaxDbiO35704
PeptideAtlasiO35704
PRIDEiO35704

PTM databases

iPTMnetiO35704
PhosphoSitePlusiO35704

Expressioni

Tissue specificityi

Expressed in a variety of tissues. Highest expression in brain, kidney and liver.

Gene expression databases

BgeeiENSMUSG00000021468 Expressed in 282 organ(s), highest expression level in skin of back
GenevisibleiO35704 MM

Interactioni

Subunit structurei

Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi234529, 3 interactors
IntActiO35704, 1 interactor
MINTiO35704
STRINGi10090.ENSMUSP00000021920

Structurei

3D structure databases

ProteinModelPortaliO35704
SMRiO35704
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1358 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00550000074872
HOGENOMiHOG000216602
HOVERGENiHBG003992
InParanoidiO35704
KOiK00654
OMAiCVGSHFI
OrthoDBiEOG091G0DOG
TreeFamiTF314877

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

O35704-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATVAEQWVL VEMVQALYEA PAYHLILEGI LILWIIRLVF SKTYKLQERS
60 70 80 90 100
DLTAKEKEEL IEEWQPEPLV PPVSKNHPAL NYNIVSGPPT HNIVVNGKEC
110 120 130 140 150
VNFASFNFLG LLANPRVKAT AFSSLKKYGV GTCGPRGFYG TFDVHLDLEE
160 170 180 190 200
RLAKFMKTEE AIIYSYGFST IASAIPAYSK RGDIIFVDSA ACFAIQKGLQ
210 220 230 240 250
ASRSDIKLFK HNDVADLERL LKEQEIEDQK NPRKARVTRR FIVVEGLYMN
260 270 280 290 300
TGTICPLPEL VKLKYKYKAR IFLEESLSFG VLGEHGRGVT EHYGISIDDI
310 320 330 340 350
DLISANMENA LASVGGFCCG RSFVVDHQRL SGQGYCFSAS LPPLLAAAAI
360 370 380 390 400
EALNIMEENP DIFAVLKKKC QNIHKSLQGV SGLKVVGESL SPALHLQLEE
410 420 430 440 450
STGSREKDVK LLQAIVDQCM DKGIALTQAR YLDKEEKCLP PPSIRVVVTV
460 470
EQTEEELQRA ASTIREAAQA VLL
Length:473
Mass (Da):52,535
Last modified:July 27, 2011 - v2
Checksum:i5B037E344DDEB4A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7EQ → DE in AAC02264 (PubMed:9405408).Curated2
Sequence conflicti85V → E in AAC02264 (PubMed:9405408).Curated1
Sequence conflicti120T → A in CAA64897 (PubMed:9363775).Curated1
Sequence conflicti120T → A in AAC02264 (PubMed:9405408).Curated1
Sequence conflicti165S → T in AAC02264 (PubMed:9405408).Curated1
Sequence conflicti171I → V in CAA64897 (PubMed:9363775).Curated1
Sequence conflicti171I → V in AAC02264 (PubMed:9405408).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95641 mRNA Translation: CAA64897.1
AF003823 mRNA Translation: AAC02264.1
AK053207 mRNA Translation: BAC35310.1
AK079578 mRNA Translation: BAC37690.1
AK084391 mRNA Translation: BAC39172.1
AK084445 mRNA Translation: BAC39185.1
BC046323 mRNA Translation: AAH46323.1
CCDSiCCDS26521.1
RefSeqiNP_033295.2, NM_009269.2
UniGeneiMm.240336

Genome annotation databases

EnsembliENSMUST00000021920; ENSMUSP00000021920; ENSMUSG00000021468
GeneIDi268656
KEGGimmu:268656
UCSCiuc007qnk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95641 mRNA Translation: CAA64897.1
AF003823 mRNA Translation: AAC02264.1
AK053207 mRNA Translation: BAC35310.1
AK079578 mRNA Translation: BAC37690.1
AK084391 mRNA Translation: BAC39172.1
AK084445 mRNA Translation: BAC39185.1
BC046323 mRNA Translation: AAH46323.1
CCDSiCCDS26521.1
RefSeqiNP_033295.2, NM_009269.2
UniGeneiMm.240336

3D structure databases

ProteinModelPortaliO35704
SMRiO35704
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234529, 3 interactors
IntActiO35704, 1 interactor
MINTiO35704
STRINGi10090.ENSMUSP00000021920

Chemistry databases

GuidetoPHARMACOLOGYi2509

PTM databases

iPTMnetiO35704
PhosphoSitePlusiO35704

Proteomic databases

EPDiO35704
MaxQBiO35704
PaxDbiO35704
PeptideAtlasiO35704
PRIDEiO35704

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021920; ENSMUSP00000021920; ENSMUSG00000021468
GeneIDi268656
KEGGimmu:268656
UCSCiuc007qnk.2 mouse

Organism-specific databases

CTDi10558
MGIiMGI:1099431 Sptlc1

Phylogenomic databases

eggNOGiKOG1358 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00550000074872
HOGENOMiHOG000216602
HOVERGENiHBG003992
InParanoidiO35704
KOiK00654
OMAiCVGSHFI
OrthoDBiEOG091G0DOG
TreeFamiTF314877

Enzyme and pathway databases

UniPathwayi
UPA00222

ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis

Miscellaneous databases

PROiPR:O35704
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021468 Expressed in 282 organ(s), highest expression level in skin of back
GenevisibleiO35704 MM

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPTC1_MOUSE
AccessioniPrimary (citable) accession number: O35704
Secondary accession number(s): O54813, Q8BH11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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