Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-2,8-sialyltransferase 8B

Gene

St8sia2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateBy similarity1
Active sitei346Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism
R-MMU-419037 NCAM1 interactions
R-MMU-975577 N-Glycan antennae elongation
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8B (EC:2.4.99.-)
Alternative name(s):
Polysialic acid synthase
Sialyltransferase 8B
Short name:
SIAT8-B
Sialyltransferase St8Sia II
Short name:
ST8SiaII
Sialyltransferase X
Short name:
STX
Gene namesi
Name:St8sia2
Synonyms:Siat8b, Stx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106020 St8sia2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini24 – 375LumenalSequence analysisAdd BLAST352

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492861 – 375Alpha-2,8-sialyltransferase 8BAdd BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi157 ↔ 307By similarity
Disulfide bondi171 ↔ 371By similarity
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi234N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO35696
PaxDbiO35696
PRIDEiO35696

PTM databases

iPTMnetiO35696
PhosphoSitePlusiO35696

Expressioni

Gene expression databases

BgeeiENSMUSG00000025789 Expressed in 177 organ(s), highest expression level in rostral migratory stream
ExpressionAtlasiO35696 baseline and differential
GenevisibleiO35696 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CADM1Q9BY672EBI-15854779,EBI-5652260From Homo sapiens.

Protein-protein interaction databases

BioGridi203244, 1 interactor
DIPiDIP-59528N
IntActiO35696, 2 interactors
STRINGi10090.ENSMUSP00000026896

Structurei

3D structure databases

ProteinModelPortaliO35696
SMRiO35696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 208Substrate bindingBy similarity3
Regioni294 – 296Substrate bindingBy similarity3
Regioni329 – 330Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOGENOMiHOG000232070
HOVERGENiHBG056466
InParanoidiO35696
KOiK06612
OMAiIHYVFDR
OrthoDBiEOG091G0AL4
TreeFamiTF352820

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35696-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS
60 70 80 90 100
KSNRAEVVIN GSSPPAVADR SNESLKHNIQ PASSKWRHNQ TLSLRIRKQI
110 120 130 140 150
LKFLDAEKDI SVLKGTLKPG DIIHYIFDRD STMNVSQNLY ELLPRTSPLK
160 170 180 190 200
NKHFQTCAIV GNSGVLLNSG CGQEIDTHSF VIRCNLAPVQ EYARDVGLKT
210 220 230 240 250
DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HGLNGSILWI PAFMARGGKE
260 270 280 290 300
RVEWVNALIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY
310 320 330 340 350
TLATRFCNQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL
360 370
EFKALKSLHE QGALKLTVGQ CDGAT
Length:375
Mass (Da):42,411
Last modified:July 27, 2011 - v2
Checksum:i34F43C519A0236CF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YVX5A0A0A6YVX5_MOUSE
Alpha-2,8-sialyltransferase 8B
St8sia2
206Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186L → R in CAA67965 (PubMed:8939967).Curated1
Sequence conflicti186L → R in CAA58548 (PubMed:8939967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99646
, X99647, X99648, X99651, X99649, X99650 Genomic DNA Translation: CAA67965.1
X83562 mRNA Translation: CAA58548.1
BC096546 mRNA Translation: AAH96546.1
CH466543 Genomic DNA Translation: EDL07123.1
CCDSiCCDS21365.1
PIRiI48686
RefSeqiNP_033207.2, NM_009181.2
UniGeneiMm.4954

Genome annotation databases

EnsembliENSMUST00000026896; ENSMUSP00000026896; ENSMUSG00000025789
GeneIDi20450
KEGGimmu:20450
UCSCiuc009hwe.2 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia II

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99646
, X99647, X99648, X99651, X99649, X99650 Genomic DNA Translation: CAA67965.1
X83562 mRNA Translation: CAA58548.1
BC096546 mRNA Translation: AAH96546.1
CH466543 Genomic DNA Translation: EDL07123.1
CCDSiCCDS21365.1
PIRiI48686
RefSeqiNP_033207.2, NM_009181.2
UniGeneiMm.4954

3D structure databases

ProteinModelPortaliO35696
SMRiO35696
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203244, 1 interactor
DIPiDIP-59528N
IntActiO35696, 2 interactors
STRINGi10090.ENSMUSP00000026896

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

PTM databases

iPTMnetiO35696
PhosphoSitePlusiO35696

Proteomic databases

MaxQBiO35696
PaxDbiO35696
PRIDEiO35696

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026896; ENSMUSP00000026896; ENSMUSG00000025789
GeneIDi20450
KEGGimmu:20450
UCSCiuc009hwe.2 mouse

Organism-specific databases

CTDi8128
MGIiMGI:106020 St8sia2

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOGENOMiHOG000232070
HOVERGENiHBG056466
InParanoidiO35696
KOiK06612
OMAiIHYVFDR
OrthoDBiEOG091G0AL4
TreeFamiTF352820

Enzyme and pathway databases

UniPathwayi
UPA00378

BRENDAi2.4.99.8 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism
R-MMU-419037 NCAM1 interactions
R-MMU-975577 N-Glycan antennae elongation

Miscellaneous databases

PROiPR:O35696
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025789 Expressed in 177 organ(s), highest expression level in rostral migratory stream
ExpressionAtlasiO35696 baseline and differential
GenevisibleiO35696 MM

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8B_MOUSE
AccessioniPrimary (citable) accession number: O35696
Secondary accession number(s): Q4VA47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again