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Protein

Synaptotagmin-3

Gene

Syt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ca2+ sensor involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain. Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. Plays a role in dendrite formation by melanocytes.By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi327Calcium 1By similarity1
Metal bindingi327Calcium 2By similarity1
Metal bindingi333Calcium 1By similarity1
Metal bindingi385Calcium 1By similarity1
Metal bindingi385Calcium 2By similarity1
Metal bindingi386Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi387Calcium 1By similarity1
Metal bindingi387Calcium 2By similarity1
Metal bindingi387Calcium 3By similarity1
Metal bindingi390Calcium 3By similarity1
Metal bindingi393Calcium 2By similarity1
Metal bindingi393Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation, Exocytosis
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-3
Alternative name(s):
Synaptotagmin III
Short name:
SytIII
Gene namesi
Name:Syt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99665 Syt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54VesicularSequence analysisAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 587CytoplasmicSequence analysisAdd BLAST512

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10C → A: Abolishes disulfide-dependent homodimerization. Abolishes disulfide-dependent homodimerization; when associated with A-21. 1 Publication1
Mutagenesisi21C → A: Abolishes disulfide-dependent homodimerization; when associated with A-10. Does not affect disulfide-dependent homodimerization; when associated with A-33. 1 Publication1
Mutagenesisi33C → A: Does not affect disulfide-dependent homodimerization; when associated with A-21. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839461 – 587Synaptotagmin-3Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei286Omega-N-methylarginineCombined sources1

Keywords - PTMi

Disulfide bond, Methylation

Proteomic databases

MaxQBiO35681
PaxDbiO35681
PeptideAtlasiO35681
PRIDEiO35681

PTM databases

iPTMnetiO35681
PhosphoSitePlusiO35681
SwissPalmiO35681

Expressioni

Gene expression databases

CleanExiMM_SYT3

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343). Can also form heterodimers with SYT6, SYT9 and SYT10 (PubMed:10531343, PubMed:10531344).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203613, 1 interactor
IntActiO35681, 6 interactors
MINTiO35681
STRINGi10090.ENSMUSP00000112432

Structurei

3D structure databases

ProteinModelPortaliO35681
SMRiO35681
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 399C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini430 – 533C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 34Cysteine motif1 PublicationAdd BLAST25

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.1 Publication
The first C2 domain mediates Ca2+-dependent phospholipid binding.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDMB Eukaryota
COG5038 LUCA
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiO35681
KOiK19903
PhylomeDBiO35681

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028682 SYT3
PANTHERiPTHR10024:SF176 PTHR10024:SF176, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O35681-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGDYEDDLC RRALILVSDL CARVRDADTN DRCQEFNELR IRGYPRGPDA
60 70 80 90 100
DISVSLLSVI VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD
110 120 130 140 150
LAPGVGLAGL VGGGGHHLGA SLGGHPLLGG PHHHGHTAHH PPFAELLEPG
160 170 180 190 200
GLGGSEPPEP SYLDMDSYPE AAVASVVAAG VKPSQTSPEL PSEGGTGSGL
210 220 230 240 250
LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL TTQADPSTEE
260 270 280 290 300
RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRGGGS GEAGAPCGRI
310 320 330 340 350
SFALRYLYGS DHLVVRILQA LDLPAKDSNG FSDPYVKIYL LPDRKKKFQT
360 370 380 390 400
KVHRKTLNPI FNETFQFSVP LAELAQRKLH FSVYDFDRFS RHDLIGQVVL
410 420 430 440 450
DNLLELAEQP PDRPLWRDIL EGGSEKADLG ELNFSLCYLP TAGRLTVTII
460 470 480 490 500
KASNLKAMDL TGFSDPYVKA SLISEGRRLK KRKTSIKKNT LNPTYNEALV
510 520 530 540 550
FDVAPESVEN VGLSIAVVDY DCIGHNEVIG VCRVGPEAAD PHGREHWAEM
560 570 580
LANPRKPVEH WHQLVEEKTL SSFTKGGKGL SEKENSE
Length:587
Mass (Da):63,254
Last modified:July 19, 2003 - v2
Checksum:i87EAEAADF22D5A4C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X9Y1G3X9Y1_MOUSE
Synaptotagmin III, isoform CRA_a
Syt3 mCG_5706
587Annotation score:
D3YYS0D3YYS0_MOUSE
Synaptotagmin-3
Syt3
50Annotation score:
D3Z1B8D3Z1B8_MOUSE
Synaptotagmin-3
Syt3
27Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111V → L in BAA08292 (Ref. 1) Curated1
Sequence conflicti232P → A in BAA08292 (Ref. 1) Curated1
Sequence conflicti312H → Q in BAA08292 (Ref. 1) Curated1
Sequence conflicti312H → Q in BAA19204 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45858 mRNA Translation: BAA08292.1
AB000893 mRNA Translation: BAA19204.1
BC051969 mRNA Translation: AAH51969.1
CCDSiCCDS21205.1
RefSeqiNP_001107588.1, NM_001114116.1
NP_057872.3, NM_016663.3
UniGeneiMm.4824

Genome annotation databases

GeneIDi20981
KEGGimmu:20981

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45858 mRNA Translation: BAA08292.1
AB000893 mRNA Translation: BAA19204.1
BC051969 mRNA Translation: AAH51969.1
CCDSiCCDS21205.1
RefSeqiNP_001107588.1, NM_001114116.1
NP_057872.3, NM_016663.3
UniGeneiMm.4824

3D structure databases

ProteinModelPortaliO35681
SMRiO35681
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203613, 1 interactor
IntActiO35681, 6 interactors
MINTiO35681
STRINGi10090.ENSMUSP00000112432

PTM databases

iPTMnetiO35681
PhosphoSitePlusiO35681
SwissPalmiO35681

Proteomic databases

MaxQBiO35681
PaxDbiO35681
PeptideAtlasiO35681
PRIDEiO35681

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20981
KEGGimmu:20981

Organism-specific databases

CTDi84258
MGIiMGI:99665 Syt3

Phylogenomic databases

eggNOGiENOG410KDMB Eukaryota
COG5038 LUCA
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiO35681
KOiK19903
PhylomeDBiO35681

Miscellaneous databases

PROiPR:O35681
SOURCEiSearch...

Gene expression databases

CleanExiMM_SYT3

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028682 SYT3
PANTHERiPTHR10024:SF176 PTHR10024:SF176, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSYT3_MOUSE
AccessioniPrimary (citable) accession number: O35681
Secondary accession number(s): P97791, Q80WV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 19, 2003
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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