UniProtKB - O35678 (MGLL_MOUSE)
Protein
Monoglyceride lipase
Gene
Mgll
Organism
Mus musculus (Mouse)
Status
Functioni
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:9341166, PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20729846). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).By similarity5 Publications
Miscellaneous
Short-term inhibition causes analgesia, while long-term inhibition causes tolerance to endocannabinoids acting on brain cannabinoid receptor CNR1, and a reduction in brain cannabinoid receptor CNR1 activity.
Catalytic activityi
- Hydrolyzes glycerol monoesters of long-chain fatty acids.2 Publications EC:3.1.1.23
: triacylglycerol degradation Pathwayi
This protein is involved in the pathway triacylglycerol degradation, which is part of Glycerolipid metabolism.2 PublicationsView all proteins of this organism that are known to be involved in the pathway triacylglycerol degradation and in Glycerolipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 122 | Nucleophile1 Publication | 1 | |
Active sitei | 239 | Charge relay system1 Publication | 1 | |
Active sitei | 269 | Charge relay system1 Publication | 1 |
GO - Molecular functioni
- acylglycerol lipase activity Source: UniProtKB
- hydrolase activity Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- acylglycerol catabolic process Source: UniProtKB
- arachidonic acid metabolic process Source: UniProtKB
- fatty acid biosynthetic process Source: UniProtKB-KW
- long-term synaptic depression Source: MGI
- negative regulation of blood vessel diameter Source: MGI
- regulation of axon extension Source: MGI
- regulation of endocannabinoid signaling pathway Source: UniProtKB
- regulation of inflammatory response Source: UniProtKB
- regulation of sensory perception of pain Source: UniProtKB
- regulation of signal transduction Source: UniProtKB
- triglyceride catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-1482883 Acyl chain remodeling of DAG and TAG R-MMU-426048 Arachidonate production from DAG |
UniPathwayi | UPA00256 |
Protein family/group databases
ESTHERi | mouse-MGLL Monoglyceridelipase_lysophospholip |
MEROPSi | S33.979 |
Chemistry databases
SwissLipidsi | SLP:000000321 SLP:000000521 |
Names & Taxonomyi
Protein namesi | Recommended name: Monoglyceride lipaseImported (EC:3.1.1.232 Publications)Short name: MGL Alternative name(s): Monoacylglycerol lipase Short name: MAGL |
Gene namesi | Name:MgllImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346042 Mgll |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Other locations
- Membrane ; Peripheral membrane protein 1 Publication
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: MGI
Other locations
- axon Source: MGI
- extrinsic component of membrane Source: UniProtKB
- membrane Source: GO_Central
- synapse Source: MGI
- varicosity Source: MGI
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
Ten-fold increase in brain 2-arachidonoylglycerol levels, combined with low levels of brain arachidonic acid.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 122 | S → A: Abolishes activity. 1 Publication | 1 | |
Mutagenesisi | 239 | D → A: Abolishes activity. 1 Publication | 1 | |
Mutagenesisi | 243 | D → A: Slightly reduces activity. 1 Publication | 1 | |
Mutagenesisi | 269 | H → A: Abolishes activity. 1 Publication | 1 | |
Mutagenesisi | 272 | H → A: Reduces activity. 1 Publication | 1 | |
Mutagenesisi | 284 | H → A: Slightly reduces activity. 1 Publication | 1 | |
Mutagenesisi | 292 | H → A: Slightly reduces activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5774 |
GuidetoPHARMACOLOGYi | 1399 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191266 | 1 – 303 | Monoglyceride lipaseAdd BLAST | 303 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 58 | Nitrated tyrosineCombined sources | 1 | |
Modified residuei | 189 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
jPOSTi | O35678 |
MaxQBi | O35678 |
PaxDbi | O35678 |
PeptideAtlasi | O35678 |
PRIDEi | O35678 |
PTM databases
iPTMneti | O35678 |
PhosphoSitePlusi | O35678 |
SwissPalmi | O35678 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000033174 Expressed in 291 organ(s), highest expression level in cochlea |
ExpressionAtlasi | O35678 baseline and differential |
Genevisiblei | O35678 MM |
Interactioni
Subunit structurei
Homodimer.By similarity
GO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGridi | 204809, 1 interactor |
IntActi | O35678, 3 interactors |
MINTi | O35678 |
STRINGi | 10090.ENSMUSP00000127374 |
Chemistry databases
BindingDBi | O35678 |
Structurei
3D structure databases
ProteinModelPortali | O35678 |
SMRi | O35678 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1455 Eukaryota COG2267 LUCA |
GeneTreei | ENSGT00390000011364 |
HOGENOMi | HOG000214242 |
HOVERGENi | HBG049220 |
InParanoidi | O35678 |
KOi | K01054 |
OrthoDBi | 919091at2759 |
PhylomeDBi | O35678 |
TreeFami | TF329184 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058 AB_hydrolase IPR000073 AB_hydrolase_1 IPR022742 Hydrolase_4 |
Pfami | View protein in Pfam PF12146 Hydrolase_4, 1 hit |
PRINTSi | PR00111 ABHYDROLASE |
SUPFAMi | SSF53474 SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120 LIPASE_SER, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O35678-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MPEASSPRRT PQNVPYQDLP HLVNADGQYL FCRYWKPSGT PKALIFVSHG
60 70 80 90 100
AGEHCGRYDE LAHMLKGLDM LVFAHDHVGH GQSEGERMVV SDFQVFVRDV
110 120 130 140 150
LQHVDTIQKD YPDVPIFLLG HSMGGAISIL VAAERPTYFS GMVLISPLVL
160 170 180 190 200
ANPESASTLK VLAAKLLNFV LPNMTLGRID SSVLSRNKSE VDLYNSDPLV
210 220 230 240 250
CRAGLKVCFG IQLLNAVARV ERAMPRLTLP FLLLQGSADR LCDSKGAYLL
260 270 280 290 300
MESSRSQDKT LKMYEGAYHV LHRELPEVTN SVLHEVNSWV SHRIAAAGAG
CPP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q3B9 | E9Q3B9_MOUSE | Monoglyceride lipase | Mgll | 319 | Annotation score: | ||
D3YYS6 | D3YYS6_MOUSE | Monoglyceride lipase | Mgll | 331 | Annotation score: | ||
Q3UFN1 | Q3UFN1_MOUSE | Monoglyceride lipase | Mgll | 258 | Annotation score: | ||
A0A0N4SUZ2 | A0A0N4SUZ2_MOUSE | Monoglyceride lipase | Mgll | 47 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010315 | 78 – 303 | VGHGQ…AGCPP → AVMLSAALQSALVIFVRLIW QIIFQGYPRGVCCGGRCHY in isoform 2. 1 PublicationAdd BLAST | 226 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001118 mRNA Translation: CAA04544.1 AJ316580 mRNA Translation: CAC69874.1 AK006949 mRNA Translation: BAB24800.1 AK131645 mRNA Translation: BAE20737.1 BC057965 mRNA Translation: AAH57965.1 |
CCDSi | CCDS20338.1 [O35678-1] |
RefSeqi | NP_001159721.1, NM_001166249.1 NP_001159722.1, NM_001166250.1 NP_001159723.1, NM_001166251.1 NP_035974.1, NM_011844.4 [O35678-1] |
UniGenei | Mm.272197 |
Genome annotation databases
Ensembli | ENSMUST00000089449; ENSMUSP00000086872; ENSMUSG00000033174 [O35678-1] ENSMUST00000113585; ENSMUSP00000109215; ENSMUSG00000033174 [O35678-1] ENSMUST00000150180; ENSMUSP00000145068; ENSMUSG00000033174 [O35678-2] |
GeneIDi | 23945 |
KEGGi | mmu:23945 |
UCSCi | uc009cvp.2 mouse [O35678-1] uc009cvq.2 mouse [O35678-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001118 mRNA Translation: CAA04544.1 AJ316580 mRNA Translation: CAC69874.1 AK006949 mRNA Translation: BAB24800.1 AK131645 mRNA Translation: BAE20737.1 BC057965 mRNA Translation: AAH57965.1 |
CCDSi | CCDS20338.1 [O35678-1] |
RefSeqi | NP_001159721.1, NM_001166249.1 NP_001159722.1, NM_001166250.1 NP_001159723.1, NM_001166251.1 NP_035974.1, NM_011844.4 [O35678-1] |
UniGenei | Mm.272197 |
3D structure databases
ProteinModelPortali | O35678 |
SMRi | O35678 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 204809, 1 interactor |
IntActi | O35678, 3 interactors |
MINTi | O35678 |
STRINGi | 10090.ENSMUSP00000127374 |
Chemistry databases
BindingDBi | O35678 |
ChEMBLi | CHEMBL5774 |
GuidetoPHARMACOLOGYi | 1399 |
SwissLipidsi | SLP:000000321 SLP:000000521 |
Protein family/group databases
ESTHERi | mouse-MGLL Monoglyceridelipase_lysophospholip |
MEROPSi | S33.979 |
PTM databases
iPTMneti | O35678 |
PhosphoSitePlusi | O35678 |
SwissPalmi | O35678 |
Proteomic databases
jPOSTi | O35678 |
MaxQBi | O35678 |
PaxDbi | O35678 |
PeptideAtlasi | O35678 |
PRIDEi | O35678 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000089449; ENSMUSP00000086872; ENSMUSG00000033174 [O35678-1] ENSMUST00000113585; ENSMUSP00000109215; ENSMUSG00000033174 [O35678-1] ENSMUST00000150180; ENSMUSP00000145068; ENSMUSG00000033174 [O35678-2] |
GeneIDi | 23945 |
KEGGi | mmu:23945 |
UCSCi | uc009cvp.2 mouse [O35678-1] uc009cvq.2 mouse [O35678-2] |
Organism-specific databases
CTDi | 11343 |
MGIi | MGI:1346042 Mgll |
Phylogenomic databases
eggNOGi | KOG1455 Eukaryota COG2267 LUCA |
GeneTreei | ENSGT00390000011364 |
HOGENOMi | HOG000214242 |
HOVERGENi | HBG049220 |
InParanoidi | O35678 |
KOi | K01054 |
OrthoDBi | 919091at2759 |
PhylomeDBi | O35678 |
TreeFami | TF329184 |
Enzyme and pathway databases
UniPathwayi | UPA00256 |
Reactomei | R-MMU-1482883 Acyl chain remodeling of DAG and TAG R-MMU-426048 Arachidonate production from DAG |
Miscellaneous databases
ChiTaRSi | Mgll mouse |
PROi | PR:O35678 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033174 Expressed in 291 organ(s), highest expression level in cochlea |
ExpressionAtlasi | O35678 baseline and differential |
Genevisiblei | O35678 MM |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058 AB_hydrolase IPR000073 AB_hydrolase_1 IPR022742 Hydrolase_4 |
Pfami | View protein in Pfam PF12146 Hydrolase_4, 1 hit |
PRINTSi | PR00111 ABHYDROLASE |
SUPFAMi | SSF53474 SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120 LIPASE_SER, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | MGLL_MOUSE | |
Accessioni | O35678Primary (citable) accession number: O35678 Secondary accession number(s): Q3V2R0, Q9D9G8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways