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Protein

Integrin beta-1-binding protein 1

Gene

Itgb1bp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMitogen
Biological processAngiogenesis, Biomineralization, Cell adhesion, Differentiation, Notch signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-1-binding protein 1
Alternative name(s):
Bodenin
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1306802 Itgb1bp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show severe defects in osteoblast mesenchymal cells to compaction, proliferation, differentiation, and mineralization and to a delay in bone nodule formation. Suffer also from an excessive microvessel growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842651 – 200Integrin beta-1-binding protein 1Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38Phosphothreonine; by CaMK2By similarity1
Modified residuei41PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Thr-38 seems to enhance integrin alpha5beta1-mediated cell adhesion. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35671
MaxQBiO35671
PaxDbiO35671
PRIDEiO35671

PTM databases

iPTMnetiO35671
PhosphoSitePlusiO35671

Expressioni

Tissue specificityi

Expressed in the brain.

Developmental stagei

First expressed in the heart primordium at E8.5.

Gene expression databases

BgeeiENSMUSG00000062352 Expressed in 288 organ(s), highest expression level in testis
CleanExiMM_ITGB1BP1
ExpressionAtlasiO35671 baseline and differential
GenevisibleiO35671 MM

Interactioni

Subunit structurei

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (via C-terminal region) with ITGB1 (via C-terminal cytoplasmic tail); the interaction prevents talin TLN1 binding to ITGB1 and KRIT1 and ITGB1 compete for the same binding site. Interacts with KRIT1 (via N-terminal NPXY motif); the interaction induces the opening conformation of KRIT1 and KRIT1 and ITGB1 compete for the same binding site. Isoform 2 does not interact with ITGB1. Interacts with CDC42 (GTP- or GDP-bound form); the interaction is increased with the CDC42-membrane bound forms and prevents both CDC42 activation and cell spreading. Interacts (via C-terminal domain region) with NME2. Interacts with FERMT2 and RAC1 (By similarity). Interacts (via N-terminus and PTB domain) with ROCK1.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075609

Structurei

3D structure databases

ProteinModelPortaliO35671
SMRiO35671
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 200PIDAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 139Interaction with KRIT1By similarity4
Regioni139 – 141Interaction with ITGB1By similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi6 – 7Nuclear localization signalBy similarity2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 55Ser/Thr-richAdd BLAST46

Phylogenomic databases

eggNOGiENOG410IFN6 Eukaryota
ENOG4111GWT LUCA
GeneTreeiENSGT00390000003990
HOGENOMiHOG000015089
HOVERGENiHBG052155
InParanoidiO35671
KOiK20058
OMAiRMLCYDD
OrthoDBiEOG091G16AY
PhylomeDBiO35671
TreeFamiTF105393

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019517 Integrin-bd_ICAP-1
IPR011993 PH-like_dom_sf
IPR006020 PTB/PI_dom
PANTHERiPTHR32055 PTHR32055, 1 hit
PfamiView protein in Pfam
PF10480 ICAP-1_inte_bdg, 1 hit
SMARTiView protein in SMART
SM00462 PTB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O35671-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS
60 70 80 90 100
GQSNSNLDTC AEFRIKYVGA IEKLAVSEGK SLEGPLDLIN YIDVAQQDGK
110 120 130 140 150
LPFVPLEEEF ILGVSKYGIK VSTTDQHGVL HRHALYLIIR MVCYDDGLGA
160 170 180 190 200
GKSLLALKTT DASNEEYSLW VYQCNSLEQA QAICKVLSTA FDSVLTSDKS
Length:200
Mass (Da):21,644
Last modified:January 1, 1998 - v1
Checksum:iF7F0EB09490AEA37
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UGH2Q3UGH2_MOUSE
Integrin beta-1-binding protein 1
Itgb1bp1
188Annotation score:
G3UX55G3UX55_MOUSE
Integrin beta-1-binding protein 1
Itgb1bp1
84Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001373 mRNA Translation: CAA04706.1
AK002786 mRNA Translation: BAB22357.1
AK004172 mRNA Translation: BAB23206.1
AK007635 mRNA Translation: BAB25151.1
AK075577 mRNA Translation: BAC35832.1
AK089937 mRNA Translation: BAC41007.1
BC028772 mRNA Translation: AAH28772.1
CCDSiCCDS25833.1
RefSeqiNP_032429.1, NM_008403.4
XP_006515054.1, XM_006514991.3
UniGeneiMm.273333

Genome annotation databases

EnsembliENSMUST00000076260; ENSMUSP00000075609; ENSMUSG00000062352
ENSMUST00000173729; ENSMUSP00000134627; ENSMUSG00000062352
ENSMUST00000232072; ENSMUSP00000156312; ENSMUSG00000062352
GeneIDi16413
KEGGimmu:16413
UCSCiuc007ndl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001373 mRNA Translation: CAA04706.1
AK002786 mRNA Translation: BAB22357.1
AK004172 mRNA Translation: BAB23206.1
AK007635 mRNA Translation: BAB25151.1
AK075577 mRNA Translation: BAC35832.1
AK089937 mRNA Translation: BAC41007.1
BC028772 mRNA Translation: AAH28772.1
CCDSiCCDS25833.1
RefSeqiNP_032429.1, NM_008403.4
XP_006515054.1, XM_006514991.3
UniGeneiMm.273333

3D structure databases

ProteinModelPortaliO35671
SMRiO35671
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075609

PTM databases

iPTMnetiO35671
PhosphoSitePlusiO35671

Proteomic databases

EPDiO35671
MaxQBiO35671
PaxDbiO35671
PRIDEiO35671

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076260; ENSMUSP00000075609; ENSMUSG00000062352
ENSMUST00000173729; ENSMUSP00000134627; ENSMUSG00000062352
ENSMUST00000232072; ENSMUSP00000156312; ENSMUSG00000062352
GeneIDi16413
KEGGimmu:16413
UCSCiuc007ndl.1 mouse

Organism-specific databases

CTDi9270
MGIiMGI:1306802 Itgb1bp1

Phylogenomic databases

eggNOGiENOG410IFN6 Eukaryota
ENOG4111GWT LUCA
GeneTreeiENSGT00390000003990
HOGENOMiHOG000015089
HOVERGENiHBG052155
InParanoidiO35671
KOiK20058
OMAiRMLCYDD
OrthoDBiEOG091G16AY
PhylomeDBiO35671
TreeFamiTF105393

Miscellaneous databases

PROiPR:O35671
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062352 Expressed in 288 organ(s), highest expression level in testis
CleanExiMM_ITGB1BP1
ExpressionAtlasiO35671 baseline and differential
GenevisibleiO35671 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019517 Integrin-bd_ICAP-1
IPR011993 PH-like_dom_sf
IPR006020 PTB/PI_dom
PANTHERiPTHR32055 PTHR32055, 1 hit
PfamiView protein in Pfam
PF10480 ICAP-1_inte_bdg, 1 hit
SMARTiView protein in SMART
SM00462 PTB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiITBP1_MOUSE
AccessioniPrimary (citable) accession number: O35671
Secondary accession number(s): Q542A8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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