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Protein

Serine/threonine-protein phosphatase with EF-hands 1

Gene

Ppef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May have a role in the recovery or adaptation response of photoreceptors. May have a role in diverse sensory neurons and in development.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
  • Mg2+By similarity

Activity regulationi

Activated by calcium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi175Manganese 1By similarity1
Metal bindingi177Manganese 1By similarity1
Metal bindingi204Manganese 1By similarity1
Metal bindingi204Manganese 2By similarity1
Metal bindingi236Manganese 2By similarity1
Active sitei237Proton donorBy similarity1
Metal bindingi288Manganese 2By similarity1
Metal bindingi404Manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi497 – 5081PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi580 – 5912PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi620 – 6313PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase with EF-hands 1 (EC:3.1.3.16)
Short name:
PPEF-1
Alternative name(s):
DRES10
Protein phosphatase with EF calcium-binding domain
Short name:
PPEF
Gene namesi
Name:Ppef1
Synonyms:Dres10, Ppef
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1097157 Ppef1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589001 – 650Serine/threonine-protein phosphatase with EF-hands 1Add BLAST650

Proteomic databases

EPDiO35655
MaxQBiO35655
PaxDbiO35655
PRIDEiO35655

PTM databases

iPTMnetiO35655
PhosphoSitePlusiO35655

Expressioni

Tissue specificityi

In the embryo it is almost exclusively expressed in the peripheral nervous system, within sensory neurons of cranial and dorsal root ganglia. Otherwise found in fetal inner ear and a small group of neurons in the midbrain/pons junction.

Developmental stagei

Up-regulated at E12.3 in dorsal root ganglia (DRG) and in some sensory cranial ganglia. A slightly decreased expression could still be detected in sensory ganglia at E16.5. It is not known if expression in sensory neurons persists in adult life.

Gene expression databases

BgeeiENSMUSG00000062168 Expressed in 24 organ(s), highest expression level in dorsal root ganglion
ExpressionAtlasiO35655 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071191

Structurei

3D structure databases

ProteinModelPortaliO35655
SMRiO35655
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 45IQAdd BLAST30
Domaini484 – 519EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini567 – 602EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini607 – 642EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 456CatalyticBy similarityAdd BLAST333

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0377 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000063173
HOGENOMiHOG000006820
HOVERGENiHBG008238
InParanoidiO35655
KOiK13807
OMAiWAFSMEN
OrthoDBiEOG091G02M5
TreeFamiTF313342

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000048 IQ_motif_EF-hand-BS
IPR029052 Metallo-depent_PP-like
IPR013235 PPP_dom
IPR012008 Ser/Thr-Pase_EF-hand_contain
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
PF00149 Metallophos, 1 hit
PF08321 PPP5, 1 hit
PIRSFiPIRSF000912 PPEF, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SM00015 IQ, 1 hit
SM00156 PP2Ac, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O35655-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGCGTSSKKG NKSKKVIKAA LVIQNWYRRY RARLRVRQHY ALAIFQSIEY
60 70 80 90 100
SDEQGQMQLS SFFSFMLENY TKTNNEDSAL VTRIFDNTRR ESQIKDRDDF
110 120 130 140 150
LGLIEVPDSY DGPRLQFPLT FTDIHILLQA FKQQQILHAH YVLEVLFEAR
160 170 180 190 200
KVLKQMPNFS HVKTFPAKEI TICGDLHGKL DDLMLIFYKN GLPSENNPYV
210 220 230 240 250
FNGDFVDRGN NSMEILMILL VCFLVYPSDL HLNRGNHEDF MMNLRYGFTK
260 270 280 290 300
EILQKYKLHG RKILQVLEEV YTWLPIGTII DNEILVIHGG ISESTDLNTL
310 320 330 340 350
HQLQRNKMKS VLMPPVLGNQ ETGEKRNKSA SNYVEPRKVE PDKTPSEDLT
360 370 380 390 400
KQEWEQIVDI LWSDPRGKKG CYPNTSRGGG CYFGPDVTSK VLSKNQLKML
410 420 430 440 450
IRSHECKPDG YEVSHDGKVI TVFSASNYYE EGSNRGAYIR LSYGTMPQFF
460 470 480 490 500
QYQVTSTSCL NPLHQRMNAM ESSAFKILKE KMISRKTDLI NAFELRDHSR
510 520 530 540 550
SGRISLAEWA FSMENILGLN LPWRSLSSHL VTIDSSGSVD YMSSFDDIRI
560 570 580 590 600
EKPTKDMKSN LTETMYRYRS DLKIIFNIID SDQSGLISMD EFRTMWKLFN
610 620 630 640 650
AHYKAHIDDS QIDELASIVD FNKDGNIDFN EFLKAFYVVH KYDKPGTSLA
Length:650
Mass (Da):75,058
Last modified:July 10, 2007 - v2
Checksum:iF5B349BE006B60F5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UQZ5Q3UQZ5_MOUSE
Serine/threonine-protein phosphatas...
Ppef1
237Annotation score:
B7ZCG2B7ZCG2_MOUSE
Serine/threonine-protein phosphatas...
Ppef1
56Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732450, AL974311 Genomic DNA Translation: CAM17008.1
AL974311, AL732450 Genomic DNA Translation: CAM19040.1
Y08234 Genomic DNA Translation: CAA69403.1
CCDSiCCDS41200.1
RefSeqiNP_035277.1, NM_011147.1
UniGeneiMm.296779

Genome annotation databases

EnsembliENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168
GeneIDi237178
KEGGimmu:237178
UCSCiuc009uto.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732450, AL974311 Genomic DNA Translation: CAM17008.1
AL974311, AL732450 Genomic DNA Translation: CAM19040.1
Y08234 Genomic DNA Translation: CAA69403.1
CCDSiCCDS41200.1
RefSeqiNP_035277.1, NM_011147.1
UniGeneiMm.296779

3D structure databases

ProteinModelPortaliO35655
SMRiO35655
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071191

PTM databases

iPTMnetiO35655
PhosphoSitePlusiO35655

Proteomic databases

EPDiO35655
MaxQBiO35655
PaxDbiO35655
PRIDEiO35655

Protocols and materials databases

DNASUi237178
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168
GeneIDi237178
KEGGimmu:237178
UCSCiuc009uto.2 mouse

Organism-specific databases

CTDi5475
MGIiMGI:1097157 Ppef1

Phylogenomic databases

eggNOGiKOG0377 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000063173
HOGENOMiHOG000006820
HOVERGENiHBG008238
InParanoidiO35655
KOiK13807
OMAiWAFSMEN
OrthoDBiEOG091G02M5
TreeFamiTF313342

Enzyme and pathway databases

ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Miscellaneous databases

PROiPR:O35655
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062168 Expressed in 24 organ(s), highest expression level in dorsal root ganglion
ExpressionAtlasiO35655 baseline and differential

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000048 IQ_motif_EF-hand-BS
IPR029052 Metallo-depent_PP-like
IPR013235 PPP_dom
IPR012008 Ser/Thr-Pase_EF-hand_contain
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
PF00149 Metallophos, 1 hit
PF08321 PPP5, 1 hit
PIRSFiPIRSF000912 PPEF, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SM00015 IQ, 1 hit
SM00156 PP2Ac, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
PS00125 SER_THR_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPE1_MOUSE
AccessioniPrimary (citable) accession number: O35655
Secondary accession number(s): A2AIC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 10, 2007
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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