UniProtKB - O35654 (DPOD2_MOUSE)
DNA polymerase delta subunit 2
Pold2
Functioni
GO - Molecular functioni
- DNA binding Source: InterPro
GO - Biological processi
- DNA biosynthetic process Source: UniProtKB
- DNA-dependent DNA replication Source: MGI
- DNA repair Source: UniProtKB-KW
- DNA replication Source: MGI
- DNA strand elongation involved in DNA replication Source: GO_Central
Keywordsi
Biological process | DNA damage, DNA excision, DNA repair, DNA replication |
Enzyme and pathway databases
Reactomei | R-MMU-110314, Recognition of DNA damage by PCNA-containing replication complex R-MMU-174411, Polymerase switching on the C-strand of the telomere R-MMU-174414, Processive synthesis on the C-strand of the telomere R-MMU-174417, Telomere C-strand (Lagging Strand) Synthesis R-MMU-174437, Removal of the Flap Intermediate from the C-strand R-MMU-5358565, Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-MMU-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169, Termination of translesion DNA synthesis R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400, Dual Incision in GG-NER R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-69091, Polymerase switching R-MMU-69166, Removal of the Flap Intermediate R-MMU-69183, Processive synthesis on the lagging strand |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase delta subunit 2Alternative name(s): DNA polymerase delta subunit p50 |
Gene namesi | Name:Pold2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097163, Pold2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Recruited to DNA damage sites within 2 hours following UV irradiation.By similarity
Nucleus
- delta DNA polymerase complex Source: UniProtKB
- nucleoplasm Source: MGI
Other locations
- DNA polymerase complex Source: GO_Central
- zeta DNA polymerase complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096167 | 1 – 469 | DNA polymerase delta subunit 2Add BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 257 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O35654 |
MaxQBi | O35654 |
PaxDbi | O35654 |
PeptideAtlasi | O35654 |
PRIDEi | O35654 |
ProteomicsDBi | 279806 |
PTM databases
iPTMneti | O35654 |
PhosphoSitePlusi | O35654 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020471, Expressed in cardiac ventricle and 286 other tissues |
ExpressionAtlasi | O35654, baseline and differential |
Genevisiblei | O35654, MM |
Interactioni
Subunit structurei
Component of both the DNA polymerase delta and DNA polymerase zeta complexes.
Component of the tetrameric DNA polymerase delta complex (Pol-delta4), which consists of POLD1/p125, POLD2/p50, POLD3/p66/p68 and POLD4/p12, with POLD1 bearing DNA polymerase and 3' to 5' proofreading exonuclease activities. Within Pol-delta4, directly interacts with POLD1, POLD3 and POLD4. Following stress caused by DNA damaging agents or by replication stress, POLD4 is degraded and Pol-delta4 is converted into a trimeric form of the complex (Pol-delta3), which consists of POLD1, POLD2 and POLD3. Pol-delta3 is the major form occurring at S phase replication sites, as well as DNA damage sites. Also observed as a dimeric complex with POLD2 (Pol-delta2 complex). Pol-delta2 is relatively insensitive to the PCNA stimulation (2-5-fold) compared to Pol-delta4 that is stimulated by over 50-fold. Contrary to the other components of Pol-delta4, does not directly interact with PCNA. As POLD1 and POLD4, directly interacts with WRNIP1; this interaction stimulates DNA polymerase delta-mediated DNA synthesis, independently of the presence of PCNA. This stimulation may be due predominantly to an increase of initiation frequency and also to increased processivity. Directly interacts with POLDIP2 and POLDIP3. Directly interacts with KCTD13/PDIP1; in the presence of PCNA, this interaction may stimulate DNA polymerase activity.
Component of the tetrameric Pol-zeta complex (Pol-zeta4), which consists of REV3L, MAD2L2, POLD2 and POLD3, with REV3L bearing DNA polymerase catalytic activity (By similarity).
Interacts with KCTD10 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 202291, 2 interactors |
ComplexPortali | CPX-2098, Delta DNA polymerase complex |
CORUMi | O35654 |
IntActi | O35654, 2 interactors |
STRINGi | 10090.ENSMUSP00000099986 |
Miscellaneous databases
RNActi | O35654, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2732, Eukaryota |
GeneTreei | ENSGT00390000006780 |
HOGENOMi | CLU_021763_0_0_1 |
InParanoidi | O35654 |
OMAi | DMPLKPN |
OrthoDBi | 736068at2759 |
TreeFami | TF101073 |
Family and domain databases
CDDi | cd07387, MPP_PolD2_C, 1 hit |
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR040663, DNA_pol_D_N IPR024826, DNA_pol_delta/II_ssu IPR041863, PolD2_C |
PANTHERi | PTHR10416, PTHR10416, 1 hit |
Pfami | View protein in Pfam PF18018, DNA_pol_D_N, 1 hit PF04042, DNA_pol_E_B, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFSEQAAQRA HTLLAPPSAS NATFARVPVA TYTNSSQPFR LGERSFNRQY
60 70 80 90 100
AHIYATRLIQ MRPFLVSRAQ QHWGSRVEVK KLCELQPGEQ CCVVGTLFKA
110 120 130 140 150
MSLQPSILRE ISEEHNLVPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV
160 170 180 190 200
SKLVTGTVLA VLGSAKDDGR FQVEDHCFAD LAPQKPVPPL DTDRFVLLVS
210 220 230 240 250
GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH
260 270 280 290 300
NTQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD
310 320 330 340 350
PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS
360 370 380 390 400
DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH
410 420 430 440 450
VYFCGNTPSF GSKIIRGPED QVVLLVAVPD FSSTQTACLV NLRSLACQPI
460
SFAGFGAEQE DLEGLGLGP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8Y5G7 | A8Y5G7_MOUSE | DNA polymerase delta subunit 2 | Pold2 | 241 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | S → T in CAA96567 (PubMed:9286699).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72486 mRNA Translation: CAA96567.1 AL627069 Genomic DNA No translation available. CH466574 Genomic DNA Translation: EDL40563.1 BC003342 mRNA Translation: AAH03342.1 |
CCDSi | CCDS24407.1 |
RefSeqi | NP_032920.2, NM_008894.2 XP_006514650.1, XM_006514587.3 |
Genome annotation databases
Ensembli | ENSMUST00000102922; ENSMUSP00000099986; ENSMUSG00000020471 |
GeneIDi | 18972 |
KEGGi | mmu:18972 |
UCSCi | uc007hxk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72486 mRNA Translation: CAA96567.1 AL627069 Genomic DNA No translation available. CH466574 Genomic DNA Translation: EDL40563.1 BC003342 mRNA Translation: AAH03342.1 |
CCDSi | CCDS24407.1 |
RefSeqi | NP_032920.2, NM_008894.2 XP_006514650.1, XM_006514587.3 |
3D structure databases
SMRi | O35654 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202291, 2 interactors |
ComplexPortali | CPX-2098, Delta DNA polymerase complex |
CORUMi | O35654 |
IntActi | O35654, 2 interactors |
STRINGi | 10090.ENSMUSP00000099986 |
PTM databases
iPTMneti | O35654 |
PhosphoSitePlusi | O35654 |
Proteomic databases
EPDi | O35654 |
MaxQBi | O35654 |
PaxDbi | O35654 |
PeptideAtlasi | O35654 |
PRIDEi | O35654 |
ProteomicsDBi | 279806 |
Protocols and materials databases
Antibodypediai | 13259, 204 antibodies |
DNASUi | 18972 |
Genome annotation databases
Ensembli | ENSMUST00000102922; ENSMUSP00000099986; ENSMUSG00000020471 |
GeneIDi | 18972 |
KEGGi | mmu:18972 |
UCSCi | uc007hxk.2, mouse |
Organism-specific databases
CTDi | 5425 |
MGIi | MGI:1097163, Pold2 |
Phylogenomic databases
eggNOGi | KOG2732, Eukaryota |
GeneTreei | ENSGT00390000006780 |
HOGENOMi | CLU_021763_0_0_1 |
InParanoidi | O35654 |
OMAi | DMPLKPN |
OrthoDBi | 736068at2759 |
TreeFami | TF101073 |
Enzyme and pathway databases
Reactomei | R-MMU-110314, Recognition of DNA damage by PCNA-containing replication complex R-MMU-174411, Polymerase switching on the C-strand of the telomere R-MMU-174414, Processive synthesis on the C-strand of the telomere R-MMU-174417, Telomere C-strand (Lagging Strand) Synthesis R-MMU-174437, Removal of the Flap Intermediate from the C-strand R-MMU-5358565, Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-MMU-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169, Termination of translesion DNA synthesis R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400, Dual Incision in GG-NER R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-69091, Polymerase switching R-MMU-69166, Removal of the Flap Intermediate R-MMU-69183, Processive synthesis on the lagging strand |
Miscellaneous databases
BioGRID-ORCSi | 18972, 21 hits in 53 CRISPR screens |
ChiTaRSi | Pold2, mouse |
PROi | PR:O35654 |
RNActi | O35654, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020471, Expressed in cardiac ventricle and 286 other tissues |
ExpressionAtlasi | O35654, baseline and differential |
Genevisiblei | O35654, MM |
Family and domain databases
CDDi | cd07387, MPP_PolD2_C, 1 hit |
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR040663, DNA_pol_D_N IPR024826, DNA_pol_delta/II_ssu IPR041863, PolD2_C |
PANTHERi | PTHR10416, PTHR10416, 1 hit |
Pfami | View protein in Pfam PF18018, DNA_pol_D_N, 1 hit PF04042, DNA_pol_E_B, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOD2_MOUSE | |
Accessioni | O35654Primary (citable) accession number: O35654 Secondary accession number(s): Q99J61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families