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Protein

Vesicular inhibitory amino acid transporter

Gene

Slc32a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.By similarity1 Publication

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: GO_Central
  • gamma-aminobutyric acid:proton symporter activity Source: MGI
  • glycine transmembrane transporter activity Source: MGI

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-425393 Transport of inorganic cations/anions and amino acids/oligopeptides
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Protein family/group databases

TCDBi2.A.18.5.3 the amino acid/auxin permease (aaap) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
mVGAT
Short name:
mVIAAT
Gene namesi
Name:Slc32a1
Synonyms:Vgat, Viaat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1194488 Slc32a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 133CytoplasmicSequence analysisAdd BLAST133
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 204Lumenal, vesicleSequence analysisAdd BLAST50
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 242CytoplasmicSequence analysisAdd BLAST17
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 265Lumenal, vesicleSequence analysis2
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 305CytoplasmicSequence analysisAdd BLAST19
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 341Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 384CytoplasmicSequence analysisAdd BLAST22
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 438Lumenal, vesicleSequence analysisAdd BLAST33
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Topological domaini460 – 461CytoplasmicSequence analysis2
Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
Topological domaini483 – 489Lumenal, vesicleSequence analysis7
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 525CytoplasmicSequence analysisAdd BLAST15

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice exhibit embryonic lethality and a cleft palate and omphalocele.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938221 – 525Vesicular inhibitory amino acid transporterAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Nitrated tyrosineCombined sources1
Glycosylationi341N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

PaxDbiO35633
PeptideAtlasiO35633
PRIDEiO35633

PTM databases

iPTMnetiO35633
PhosphoSitePlusiO35633
SwissPalmiO35633

Expressioni

Tissue specificityi

Brain and retina. Localized in horizontal cell tips at both rod and cone terminals.1 Publication

Gene expression databases

BgeeiENSMUSG00000037771 Expressed in 125 organ(s), highest expression level in olfactory bulb
ExpressionAtlasiO35633 baseline and differential
GenevisibleiO35633 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036299

Structurei

3D structure databases

ProteinModelPortaliO35633
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4303 Eukaryota
COG0814 LUCA
GeneTreeiENSGT00490000043380
HOGENOMiHOG000220837
HOVERGENiHBG061364
InParanoidiO35633
KOiK15015
OMAiNWSHIAA
OrthoDBiEOG091G05MY
PhylomeDBiO35633
TreeFamiTF312818

Family and domain databases

InterProiView protein in InterPro
IPR013057 AA_transpt_TM
PfamiView protein in Pfam
PF01490 Aa_trans, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O35633-1) [UniParc]FASTAAdd to basket
Also known as: b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ
110 120 130 140 150
AVGAGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYGGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,381
Last modified:March 28, 2003 - v3
Checksum:iEBD63E01A4B54C07
GO
Isoform 2 (identifier: O35633-2) [UniParc]FASTAAdd to basket
Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     515-525: LIEAYRTNAED → KFAGLET

Show »
Length:521
Mass (Da):56,852
Checksum:iD4AA407244DD3C7B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti432G → E in CAA04864 (PubMed:9395291).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007063515 – 525LIEAYRTNAED → KFAGLET in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080232 Genomic DNA Translation: BAC44888.1
AB080232 Genomic DNA Translation: BAC44889.1
AJ001598 mRNA Translation: CAA04864.1
BC052020 mRNA Translation: AAH52020.1
CCDSiCCDS38309.1 [O35633-1]
RefSeqiNP_033534.2, NM_009508.2 [O35633-1]
UniGeneiMm.143404
Mm.413854

Genome annotation databases

EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771 [O35633-1]
GeneIDi22348
KEGGimmu:22348
UCSCiuc008nqk.1 mouse [O35633-1]
uc029uhu.1 mouse [O35633-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080232 Genomic DNA Translation: BAC44888.1
AB080232 Genomic DNA Translation: BAC44889.1
AJ001598 mRNA Translation: CAA04864.1
BC052020 mRNA Translation: AAH52020.1
CCDSiCCDS38309.1 [O35633-1]
RefSeqiNP_033534.2, NM_009508.2 [O35633-1]
UniGeneiMm.143404
Mm.413854

3D structure databases

ProteinModelPortaliO35633
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036299

Protein family/group databases

TCDBi2.A.18.5.3 the amino acid/auxin permease (aaap) family

PTM databases

iPTMnetiO35633
PhosphoSitePlusiO35633
SwissPalmiO35633

Proteomic databases

PaxDbiO35633
PeptideAtlasiO35633
PRIDEiO35633

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045738; ENSMUSP00000036299; ENSMUSG00000037771 [O35633-1]
GeneIDi22348
KEGGimmu:22348
UCSCiuc008nqk.1 mouse [O35633-1]
uc029uhu.1 mouse [O35633-2]

Organism-specific databases

CTDi140679
MGIiMGI:1194488 Slc32a1

Phylogenomic databases

eggNOGiKOG4303 Eukaryota
COG0814 LUCA
GeneTreeiENSGT00490000043380
HOGENOMiHOG000220837
HOVERGENiHBG061364
InParanoidiO35633
KOiK15015
OMAiNWSHIAA
OrthoDBiEOG091G05MY
PhylomeDBiO35633
TreeFamiTF312818

Enzyme and pathway databases

ReactomeiR-MMU-425393 Transport of inorganic cations/anions and amino acids/oligopeptides
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Miscellaneous databases

PROiPR:O35633
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037771 Expressed in 125 organ(s), highest expression level in olfactory bulb
ExpressionAtlasiO35633 baseline and differential
GenevisibleiO35633 MM

Family and domain databases

InterProiView protein in InterPro
IPR013057 AA_transpt_TM
PfamiView protein in Pfam
PF01490 Aa_trans, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVIAAT_MOUSE
AccessioniPrimary (citable) accession number: O35633
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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