UniProtKB - O35627 (NR1I3_MOUSE)
Nuclear receptor subfamily 1 group I member 3
Nr1i3
Functioni
Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 171 | Important for TCPOBOP recognition | 1 | |
Sitei | 175 | Important for TCPOBOP recognition | 1 | |
Sitei | 216 | Important for TCPOBOP recognition | 1 | |
Sitei | 227 | Important for TCPOBOP recognition | 1 | |
Sitei | 244 | Important for TCPOBOP recognition | 1 | |
Sitei | 336 | Important for TCPOBOP recognition | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 18 – 93 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 21 – 41 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 57 – 81 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- nuclear receptor activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- cell differentiation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- osteoblast differentiation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 1 group I member 3Alternative name(s): Constitutive androstane receptor Short name: CAR Orphan nuclear receptor MB67 |
Gene namesi | Name:Nr1i3 Synonyms:Car |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346307, Nr1i3 |
VEuPathDBi | HostDB:ENSMUSG00000005677 |
Subcellular locationi
Cytoplasm and Cytosol
Cytoskeleton
- cytoskeleton By similarity
Nucleus
Note: Recruited to the cytoplasm by DNAJC7.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 171 | F → W: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 175 | N → F: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 216 | L → F: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 227 | F → W: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 234 | Y → A: No effect on binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 244 | F → A: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 336 | Y → A: Diminished binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 | |
Mutagenesisi | 346 | L → F: Dramatic increase in binding NCOA2. Little effect on binding of coactivator NCOA2 in the presence of TCPOBOP. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3069 |
DrugCentrali | O35627 |
GuidetoPHARMACOLOGYi | 607 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053554 | 1 – 358 | Nuclear receptor subfamily 1 group I member 3Add BLAST | 358 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | Phosphothreonine; by PKCBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O35627 |
PRIDEi | O35627 |
ProteomicsDBi | 295523 [O35627-1] 295524 [O35627-2] |
PTM databases
iPTMneti | O35627 |
PhosphoSitePlusi | O35627 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000005677, Expressed in cardiac ventricle and 104 other tissues |
Genevisiblei | O35627, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 198489, 13 interactors |
IntActi | O35627, 1 interactor |
STRINGi | 10090.ENSMUSP00000005820 |
Chemistry databases
BindingDBi | O35627 |
Miscellaneous databases
RNActi | O35627, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O35627 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O35627 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 119 – 358 | NR LBDPROSITE-ProRule annotationAdd BLAST | 240 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 41 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 57 – 81 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000160641 |
HOGENOMi | CLU_007368_12_0_1 |
InParanoidi | O35627 |
OMAi | ALEICHI |
OrthoDBi | 297114at2759 |
PhylomeDBi | O35627 |
TreeFami | TF316304 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50248 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001728, ThyrH_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER PR00546, THYROIDHORMR |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTAMLTLETM ASEEEYGPRN CVVCGDRATG YHFHALTCEG CKGFFRRTVS
60 70 80 90 100
KTIGPICPFA GRCEVSKAQR RHCPACRLQK CLNVGMRKDM ILSAEALALR
110 120 130 140 150
RARQAQRRAE KASLQLNQQQ KELVQILLGA HTRHVGPMFD QFVQFKPPAY
160 170 180 190 200
LFMHHRPFQP RGPVLPLLTH FADINTFMVQ QIIKFTKDLP LFRSLTMEDQ
210 220 230 240 250
ISLLKGAAVE ILHISLNTTF CLQTENFFCG PLCYKMEDAV HAGFQYEFLE
260 270 280 290 300
SILHFHKNLK GLHLQEPEYV LMAATALFSP DRPGVTQREE IDQLQEEMAL
310 320 330 340 350
ILNNHIMEQQ SRLQSRFLYA KLMGLLADLR SINNAYSYEL QRLEELSAMT
PLLGEICS
The sequence of this isoform differs from the canonical sequence as follows:
281-286: DRPGVT → GFCMQS
287-358: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5FW96 | Q5FW96_MOUSE | Nuclear receptor subfamily 1 group ... | Nr1i3 | 357 | Annotation score: | ||
Q3UEP1 | Q3UEP1_MOUSE | Nuclear receptor subfamily 1 group ... | Nr1i3 | 221 | Annotation score: | ||
M0QWI8 | M0QWI8_MOUSE | Nuclear receptor subfamily 1 group ... | Nr1i3 | 95 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 138 | M → L in AAC53349 (PubMed:9295294).Curated | 1 | |
Sequence conflicti | 138 | M → L in AAC53350 (PubMed:9295294).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003671 | 281 – 286 | DRPGVT → GFCMQS in isoform CAR2. 1 Publication | 6 | |
Alternative sequenceiVSP_003672 | 287 – 358 | Missing in isoform CAR2. 1 PublicationAdd BLAST | 72 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF009327 mRNA Translation: AAC53349.1 AF009328 mRNA Translation: AAC53350.1 AK133515 mRNA Translation: BAE21697.1 CH466520 Genomic DNA Translation: EDL39126.1 |
CCDSi | CCDS15480.1 [O35627-1] CCDS56654.1 [O35627-2] |
RefSeqi | NP_001229991.1, NM_001243062.1 [O35627-2] NP_001229992.1, NM_001243063.1 NP_033933.2, NM_009803.5 [O35627-1] |
Genome annotation databases
Ensembli | ENSMUST00000005820; ENSMUSP00000005820; ENSMUSG00000005677 [O35627-1] ENSMUST00000075469; ENSMUSP00000074915; ENSMUSG00000005677 [O35627-2] |
GeneIDi | 12355 |
KEGGi | mmu:12355 |
UCSCi | uc007dnf.2, mouse [O35627-1] uc007dng.2, mouse [O35627-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF009327 mRNA Translation: AAC53349.1 AF009328 mRNA Translation: AAC53350.1 AK133515 mRNA Translation: BAE21697.1 CH466520 Genomic DNA Translation: EDL39126.1 |
CCDSi | CCDS15480.1 [O35627-1] CCDS56654.1 [O35627-2] |
RefSeqi | NP_001229991.1, NM_001243062.1 [O35627-2] NP_001229992.1, NM_001243063.1 NP_033933.2, NM_009803.5 [O35627-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XLS | X-ray | 2.96 | E/F/G/H | 117-358 | [»] | |
1XNX | X-ray | 2.90 | A/B | 109-358 | [»] | |
SMRi | O35627 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198489, 13 interactors |
IntActi | O35627, 1 interactor |
STRINGi | 10090.ENSMUSP00000005820 |
Chemistry databases
BindingDBi | O35627 |
ChEMBLi | CHEMBL3069 |
DrugCentrali | O35627 |
GuidetoPHARMACOLOGYi | 607 |
PTM databases
iPTMneti | O35627 |
PhosphoSitePlusi | O35627 |
Proteomic databases
PaxDbi | O35627 |
PRIDEi | O35627 |
ProteomicsDBi | 295523 [O35627-1] 295524 [O35627-2] |
Protocols and materials databases
Antibodypediai | 20508, 456 antibodies from 32 providers |
DNASUi | 12355 |
Genome annotation databases
Ensembli | ENSMUST00000005820; ENSMUSP00000005820; ENSMUSG00000005677 [O35627-1] ENSMUST00000075469; ENSMUSP00000074915; ENSMUSG00000005677 [O35627-2] |
GeneIDi | 12355 |
KEGGi | mmu:12355 |
UCSCi | uc007dnf.2, mouse [O35627-1] uc007dng.2, mouse [O35627-2] |
Organism-specific databases
CTDi | 9970 |
MGIi | MGI:1346307, Nr1i3 |
VEuPathDBi | HostDB:ENSMUSG00000005677 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000160641 |
HOGENOMi | CLU_007368_12_0_1 |
InParanoidi | O35627 |
OMAi | ALEICHI |
OrthoDBi | 297114at2759 |
PhylomeDBi | O35627 |
TreeFami | TF316304 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 12355, 1 hit in 66 CRISPR screens |
ChiTaRSi | Nr1i3, mouse |
EvolutionaryTracei | O35627 |
PROi | PR:O35627 |
RNActi | O35627, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005677, Expressed in cardiac ventricle and 104 other tissues |
Genevisiblei | O35627, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50248 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001728, ThyrH_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER PR00546, THYROIDHORMR |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR1I3_MOUSE | |
Accessioni | O35627Primary (citable) accession number: O35627 Secondary accession number(s): O35628, Q3V008 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families