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Protein

Phosphomannomutase 1

Gene

Pmm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.1 Publication

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Cofactori

Mg2+By similarity

Activity regulationi

IMP, a metabolite whose concentration is elevated in anoxia, inhibits phosphomannomutase and phosphoglucomutase activities and strongly enhances glucose-1,6-bisphosphatase activity.1 Publication

Kineticsi

  1. KM=17 µM for glucose-1,6-bisphosphate in the presence of 1 µM IMP1 Publication
  2. KM=40 µM for glucose-1,6-bisphosphate in the presence of 20 µM IMP1 Publication
  1. Vmax=2.1 µmol/min/mg enzyme with glucose-1,6-bisphosphate as substrate1 Publication

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi), Mannose-6-phosphate isomerase (Mpi)
  2. Phosphomannomutase 2 (Pmm2), Phosphomannomutase 1 (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm2), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm1)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19NucleophileBy similarity1
Metal bindingi19MagnesiumBy similarity1
Active sitei21Proton donor/acceptorSequence analysis1
Metal bindingi21Magnesium; via carbonyl oxygenBy similarity1
Binding sitei28SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei143SubstrateBy similarity1
Binding sitei150SubstrateBy similarity1
Binding sitei186Substrate; via amide nitrogenBy similarity1
Binding sitei188SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Metal bindingi218MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to leukemia inhibitory factor Source: MGI
  • GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  • mannose metabolic process Source: UniProtKB
  • protein N-linked glycosylation Source: GO_Central
  • protein targeting to ER Source: GO_Central

Keywordsi

Molecular functionIsomerase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-446205 Synthesis of GDP-mannose
UniPathwayi
UPA00126;UER00424

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase 1 (EC:5.4.2.8)
Short name:
PMM 1
Gene namesi
Name:Pmm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1353418 Pmm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile and develop normally.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001996932 – 262Phosphomannomutase 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei242PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35621
PaxDbiO35621
PeptideAtlasiO35621
PRIDEiO35621

PTM databases

iPTMnetiO35621
PhosphoSitePlusiO35621

Expressioni

Tissue specificityi

Present in brain, where it is restricted to neuronal cell bodies. Present at lower levels in pancreas, liver, lung, gonads, uterus, adrenal glands and pituitary (at protein level). Undetectable in intestine.1 Publication

Developmental stagei

Highly expressed at E17 in most organs (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022474 Expressed in 288 organ(s), highest expression level in morula
CleanExiMM_PMM1
ExpressionAtlasiO35621 baseline and differential
GenevisibleiO35621 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023112

Structurei

3D structure databases

ProteinModelPortaliO35621
SMRiO35621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.Curated

Phylogenomic databases

eggNOGiKOG3189 Eukaryota
COG0561 LUCA
GeneTreeiENSGT00390000002918
HOGENOMiHOG000181843
HOVERGENiHBG009971
InParanoidiO35621
KOiK17497
OMAiNDYEIYD
OrthoDBiEOG091G0J2S
PhylomeDBiO35621
TreeFamiTF300874

Family and domain databases

CDDicd02585 HAD_PMM, 1 hit
Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006379 HAD-SF_hydro_IIB
IPR023214 HAD_sf
IPR005002 PMM
PANTHERiPTHR10466 PTHR10466, 1 hit
PfamiView protein in Pfam
PF03332 PMM, 1 hit
SFLDiSFLDG01143 C2.B.3:_Phosphomannomutase_Lik, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01484 HAD-SF-IIB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O35621-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVAVEGARR KERILCLFDV DGTLTPARQK IDPEVSAFLQ KLRSRVQIGV
60 70 80 90 100
VGGSDYSKIA EQLGEGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL
110 120 130 140 150
GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNVSP IGRSCTLEER
160 170 180 190 200
IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY
210 220 230 240 250
CLDSLDEDSF DIIHFFGNET SPGGNDFEIY ADPRTVGHSV VSPQDTVQRC
260
RELFFPETAH EA
Length:262
Mass (Da):29,775
Last modified:January 1, 1998 - v1
Checksum:i2967154C0FF292B0
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q91W01Q91W01_MOUSE
Phosphomannomutase
Pmm1
202Annotation score:
Q9D6D9Q9D6D9_MOUSE
Phosphomannomutase
Pmm1
165Annotation score:
A0A2R8W6J8A0A2R8W6J8_MOUSE
Phosphomannomutase
Pmm1
228Annotation score:
D6RG76D6RG76_MOUSE
Phosphomannomutase 1
Pmm1 mCG_8801
98Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007267 mRNA Translation: AAB62943.1
CCDSiCCDS27678.1
RefSeqiNP_038900.1, NM_013872.4
UniGeneiMm.18939

Genome annotation databases

EnsembliENSMUST00000023112; ENSMUSP00000023112; ENSMUSG00000022474
GeneIDi29858
KEGGimmu:29858
UCSCiuc007wxt.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007267 mRNA Translation: AAB62943.1
CCDSiCCDS27678.1
RefSeqiNP_038900.1, NM_013872.4
UniGeneiMm.18939

3D structure databases

ProteinModelPortaliO35621
SMRiO35621
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023112

PTM databases

iPTMnetiO35621
PhosphoSitePlusiO35621

Proteomic databases

EPDiO35621
PaxDbiO35621
PeptideAtlasiO35621
PRIDEiO35621

Protocols and materials databases

DNASUi29858
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023112; ENSMUSP00000023112; ENSMUSG00000022474
GeneIDi29858
KEGGimmu:29858
UCSCiuc007wxt.3 mouse

Organism-specific databases

CTDi5372
MGIiMGI:1353418 Pmm1

Phylogenomic databases

eggNOGiKOG3189 Eukaryota
COG0561 LUCA
GeneTreeiENSGT00390000002918
HOGENOMiHOG000181843
HOVERGENiHBG009971
InParanoidiO35621
KOiK17497
OMAiNDYEIYD
OrthoDBiEOG091G0J2S
PhylomeDBiO35621
TreeFamiTF300874

Enzyme and pathway databases

UniPathwayi
UPA00126;UER00424

ReactomeiR-MMU-446205 Synthesis of GDP-mannose

Miscellaneous databases

ChiTaRSiPmm1 mouse
PROiPR:O35621
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022474 Expressed in 288 organ(s), highest expression level in morula
CleanExiMM_PMM1
ExpressionAtlasiO35621 baseline and differential
GenevisibleiO35621 MM

Family and domain databases

CDDicd02585 HAD_PMM, 1 hit
Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006379 HAD-SF_hydro_IIB
IPR023214 HAD_sf
IPR005002 PMM
PANTHERiPTHR10466 PTHR10466, 1 hit
PfamiView protein in Pfam
PF03332 PMM, 1 hit
SFLDiSFLDG01143 C2.B.3:_Phosphomannomutase_Lik, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01484 HAD-SF-IIB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPMM1_MOUSE
AccessioniPrimary (citable) accession number: O35621
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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