UniProtKB - O35608 (ANGP2_MOUSE)
Protein
Angiopoietin-2
Gene
Angpt2
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 429 | CalciumBy similarity | 1 | |
Metal bindingi | 431 | CalciumBy similarity | 1 | |
Metal bindingi | 433 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 435 | Calcium; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- receptor antagonist activity Source: MGI
- receptor tyrosine kinase binding Source: MGI
- signaling receptor binding Source: GO_Central
- vascular endothelial growth factor receptor binding Source: MGI
GO - Biological processi
- angiogenesis Source: MGI
- animal organ regeneration Source: Ensembl
- blood vessel morphogenesis Source: MGI
- blood vessel remodeling Source: DFLAT
- cellular response to growth factor stimulus Source: Ensembl
- endoderm development Source: MGI
- gene expression Source: MGI
- germ cell development Source: Ensembl
- glomerulus vasculature development Source: MGI
- hemopoiesis Source: DFLAT
- maternal process involved in female pregnancy Source: Ensembl
- negative regulation of angiogenesis Source: MGI
- negative regulation of blood vessel endothelial cell migration Source: MGI
- negative regulation of cell-substrate adhesion Source: MGI
- negative regulation of positive chemotaxis Source: MGI
- positive regulation of angiogenesis Source: MGI
- regulation of angiogenesis Source: DFLAT
- response to activity Source: Ensembl
- response to glucose Source: Ensembl
- response to hypoxia Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- response to organic cyclic compound Source: Ensembl
- response to radiation Source: Ensembl
- Tie signaling pathway Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Angiogenesis, Differentiation |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-210993, Tie2 Signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Angiopoietin-2Short name: ANG-2 |
Gene namesi | Name:Angpt2 Synonyms:Agpt2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1202890, Angpt2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- collagen-containing extracellular matrix Source: GO_Central
- extracellular space Source: BHF-UCL
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cell projection Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000009114 | 19 – 496 | Angiopoietin-2Add BLAST | 478 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 151 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 240 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 284 ↔ 313 | PROSITE-ProRule annotation | ||
Glycosylationi | 304 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 433 ↔ 435 | PROSITE-ProRule annotation | ||
Disulfide bondi | 437 ↔ 450 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | O35608 |
PaxDbi | O35608 |
PRIDEi | O35608 |
PTM databases
GlyGeni | O35608, 6 sites |
iPTMneti | O35608 |
MetOSitei | O35608 |
PhosphoSitePlusi | O35608 |
Expressioni
Tissue specificityi
Expressed only at sites of vascular remodeling.
Gene expression databases
Bgeei | ENSMUSG00000031465, Expressed in decidua and 221 other tissues |
ExpressionAtlasi | O35608, baseline and differential |
Genevisiblei | O35608, MM |
Interactioni
Subunit structurei
Interacts with TEK/TIE2, competing for the same binding site as ANGPT1.
By similarityGO - Molecular functioni
- receptor antagonist activity Source: MGI
- receptor tyrosine kinase binding Source: MGI
- signaling receptor binding Source: GO_Central
- vascular endothelial growth factor receptor binding Source: MGI
Protein-protein interaction databases
IntActi | O35608, 1 interactor |
STRINGi | 10090.ENSMUSP00000033846 |
Miscellaneous databases
RNActi | O35608, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 275 – 495 | Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST | 221 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 159 – 256 | Sequence analysisAdd BLAST | 98 |
Domaini
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.By similarity
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | KOG2579, Eukaryota |
GeneTreei | ENSGT00940000158430 |
HOGENOMi | CLU_038628_3_1_1 |
InParanoidi | O35608 |
OMAi | LPETDNC |
OrthoDBi | 357340at2759 |
PhylomeDBi | O35608 |
TreeFami | TF336658 |
Family and domain databases
CDDi | cd00087, FReD, 1 hit |
Gene3Di | 3.90.215.10, 1 hit 4.10.530.10, 1 hit |
InterProi | View protein in InterPro IPR028844, Ang-2 IPR036056, Fibrinogen-like_C IPR014716, Fibrinogen_a/b/g_C_1 IPR014715, Fibrinogen_a/b/g_C_2 IPR002181, Fibrinogen_a/b/g_C_dom IPR020837, Fibrinogen_CS |
PANTHERi | PTHR19143:SF199, PTHR19143:SF199, 1 hit |
Pfami | View protein in Pfam PF00147, Fibrinogen_C, 1 hit |
SMARTi | View protein in SMART SM00186, FBG, 1 hit |
SUPFAMi | SSF56496, SSF56496, 1 hit |
PROSITEi | View protein in PROSITE PS00514, FIBRINOGEN_C_1, 1 hit PS51406, FIBRINOGEN_C_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O35608-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWQIIFLTFG WDLVLASAYS NFRKSVDSTG RRQYQVQNGP CSYTFLLPET
60 70 80 90 100
DSCRSSSSPY MSNAVQRDAP LDYDDSVQRL QVLENILENN TQWLMKLENY
110 120 130 140 150
IQDNMKKEMV EIQQNVVQNQ TAVMIEIGTS LLNQTAAQTR KLTDVEAQVL
160 170 180 190 200
NQTTRLELQL LQHSISTNKL EKQILDQTSE INKLQNKNSF LEQKVLDMEG
210 220 230 240 250
KHSEQLQSMK EQKDELQVLV SKQSSVIDEL EKKLVTATVN NSLLQKQQHD
260 270 280 290 300
LMETVNSLLT MMSSPNSKSS VAIRKEEQTT FRDCAEIFKS GLTTSGIYTL
310 320 330 340 350
TFPNSTEEIK AYCDMDVGGG GWTVIQHRED GSVDFQRTWK EYKEGFGSPL
360 370 380 390 400
GEYWLGNEFV SQLTGQHRYV LKIQLKDWEG NEAHSLYDHF YLAGEESNYR
410 420 430 440 450
IHLTGLTGTA GKISSISQPG SDFSTKDSDN DKCICKCSQM LSGGWWFDAC
460 470 480 490
GPSNLNGQYY PQKQNTNKFN GIKWYYWKGS GYSLKATTMM IRPADF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 348 | S → N in AAB63189 (PubMed:9204896).Curated | 1 | |
Sequence conflicti | 411 | G → A in AAB63189 (PubMed:9204896).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004326 mRNA Translation: AAB63189.1 AK019860 mRNA Translation: BAB31887.1 AK048622 mRNA Translation: BAC33396.1 AK156132 mRNA Translation: BAE33599.1 BC027216 mRNA Translation: AAH27216.1 |
CCDSi | CCDS22125.1 |
RefSeqi | NP_031452.2, NM_007426.4 |
Genome annotation databases
Ensembli | ENSMUST00000033846; ENSMUSP00000033846; ENSMUSG00000031465 |
GeneIDi | 11601 |
KEGGi | mmu:11601 |
UCSCi | uc009kzu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004326 mRNA Translation: AAB63189.1 AK019860 mRNA Translation: BAB31887.1 AK048622 mRNA Translation: BAC33396.1 AK156132 mRNA Translation: BAE33599.1 BC027216 mRNA Translation: AAH27216.1 |
CCDSi | CCDS22125.1 |
RefSeqi | NP_031452.2, NM_007426.4 |
3D structure databases
SMRi | O35608 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O35608, 1 interactor |
STRINGi | 10090.ENSMUSP00000033846 |
PTM databases
GlyGeni | O35608, 6 sites |
iPTMneti | O35608 |
MetOSitei | O35608 |
PhosphoSitePlusi | O35608 |
Proteomic databases
MaxQBi | O35608 |
PaxDbi | O35608 |
PRIDEi | O35608 |
Protocols and materials databases
Antibodypediai | 4319, 591 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033846; ENSMUSP00000033846; ENSMUSG00000031465 |
GeneIDi | 11601 |
KEGGi | mmu:11601 |
UCSCi | uc009kzu.2, mouse |
Organism-specific databases
CTDi | 285 |
MGIi | MGI:1202890, Angpt2 |
Phylogenomic databases
eggNOGi | KOG2579, Eukaryota |
GeneTreei | ENSGT00940000158430 |
HOGENOMi | CLU_038628_3_1_1 |
InParanoidi | O35608 |
OMAi | LPETDNC |
OrthoDBi | 357340at2759 |
PhylomeDBi | O35608 |
TreeFami | TF336658 |
Enzyme and pathway databases
Reactomei | R-MMU-210993, Tie2 Signaling |
Miscellaneous databases
BioGRID-ORCSi | 11601, 1 hit in 19 CRISPR screens |
PROi | PR:O35608 |
RNActi | O35608, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031465, Expressed in decidua and 221 other tissues |
ExpressionAtlasi | O35608, baseline and differential |
Genevisiblei | O35608, MM |
Family and domain databases
CDDi | cd00087, FReD, 1 hit |
Gene3Di | 3.90.215.10, 1 hit 4.10.530.10, 1 hit |
InterProi | View protein in InterPro IPR028844, Ang-2 IPR036056, Fibrinogen-like_C IPR014716, Fibrinogen_a/b/g_C_1 IPR014715, Fibrinogen_a/b/g_C_2 IPR002181, Fibrinogen_a/b/g_C_dom IPR020837, Fibrinogen_CS |
PANTHERi | PTHR19143:SF199, PTHR19143:SF199, 1 hit |
Pfami | View protein in Pfam PF00147, Fibrinogen_C, 1 hit |
SMARTi | View protein in SMART SM00186, FBG, 1 hit |
SUPFAMi | SSF56496, SSF56496, 1 hit |
PROSITEi | View protein in PROSITE PS00514, FIBRINOGEN_C_1, 1 hit PS51406, FIBRINOGEN_C_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ANGP2_MOUSE | |
Accessioni | O35608Primary (citable) accession number: O35608 Secondary accession number(s): Q3U1A1, Q9D2D2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | April 26, 2004 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot