UniProtKB - O35599 (OPSG_MOUSE)
Protein
Medium-wave-sensitive opsin 1
Gene
Opn1mw
Organism
Mus musculus (Mouse)
Status
Functioni
Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.1 Publication
GO - Molecular functioni
- G protein-coupled photoreceptor activity Source: GO_Central
- identical protein binding Source: UniProtKB
- photoreceptor activity Source: MGI
GO - Biological processi
- cellular response to light stimulus Source: GO_Central
- G protein-coupled receptor signaling pathway Source: GO_Central
- phototransduction Source: GO_Central
- positive regulation of cytokinesis Source: MGI
- protein-chromophore linkage Source: UniProtKB-KW
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | Sensory transduction, Vision |
Ligand | Chromophore |
Enzyme and pathway databases
Reactomei | R-MMU-2187335, The retinoid cycle in cones (daylight vision) R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Names & Taxonomyi
Protein namesi | Recommended name: Medium-wave-sensitive opsin 1Alternative name(s): Green cone photoreceptor pigment Green-sensitive opsin Short name: M opsin Medium wavelength-sensitive cone opsin |
Gene namesi | Name:Opn1mw Synonyms:Gcp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097692, Opn1mw |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- photoreceptor outer segment Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 47 | ExtracellularSequence analysisAdd BLAST | 47 | |
Transmembranei | 48 – 72 | Helical; Name=1Sequence analysisAdd BLAST | 25 | |
Topological domaini | 73 – 84 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 85 – 110 | Helical; Name=2Sequence analysisAdd BLAST | 26 | |
Topological domaini | 111 – 124 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 125 – 144 | Helical; Name=3Sequence analysisAdd BLAST | 20 | |
Topological domaini | 145 – 163 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 164 – 187 | Helical; Name=4Sequence analysisAdd BLAST | 24 | |
Topological domaini | 188 – 213 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 214 – 241 | Helical; Name=5Sequence analysisAdd BLAST | 28 | |
Topological domaini | 242 – 263 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 264 – 287 | Helical; Name=6Sequence analysisAdd BLAST | 24 | |
Topological domaini | 288 – 295 | ExtracellularSequence analysis | 8 | |
Transmembranei | 296 – 320 | Helical; Name=7Sequence analysisAdd BLAST | 25 | |
Topological domaini | 321 – 359 | CytoplasmicSequence analysisAdd BLAST | 39 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197786 | 1 – 359 | Medium-wave-sensitive opsin 1Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 29 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 121 ↔ 198 | PROSITE-ProRule annotation | ||
Modified residuei | 307 | N6-(retinylidene)lysine | 1 |
Post-translational modificationi
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | O35599 |
PRIDEi | O35599 |
PTM databases
GlyGeni | O35599, 1 site |
PhosphoSitePlusi | O35599 |
Expressioni
Tissue specificityi
Expressed in retina (at protein level) (PubMed:30948514). Expressed in cone photoreceptor cells (at protein level) (PubMed:11055434).2 Publications
Developmental stagei
Expressed in the retinal outer nuclear layer and the inner nuclear layer at postnatal day 10.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031394, Expressed in retinal neural layer and 43 other tissues |
ExpressionAtlasi | O35599, baseline and differential |
Genevisiblei | O35599, MM |
Interactioni
Subunit structurei
Monomer. Homodimer. Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033771 |
Miscellaneous databases
RNActi | O35599, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 38 | Required for 11-cis-retinal regenerationBy similarityAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00960000186618 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | O35599 |
OMAi | QRWGPQR |
OrthoDBi | 940057at2759 |
PhylomeDBi | O35599 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR000378, Opsin_red/grn IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00575, OPSINREDGRN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O35599-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQRLTGEQT LDHYEDSTHA SIFTYTNSNS TKGPFEGPNY HIAPRWVYHL
60 70 80 90 100
TSTWMILVVV ASVFTNGLVL AATMRFKKLR HPLNWILVNL AVADLAETII
110 120 130 140 150
ASTISVVNQI YGYFVLGHPL CVIEGYIVSL CGITGLWSLA IISWERWLVV
160 170 180 190 200
CKPFGNVRFD AKLATVGIVF SWVWAAIWTA PPIFGWSRYW PYGLKTSCGP
210 220 230 240 250
DVFSGTSYPG VQSYMMVLMV TCCIFPLSII VLCYLQVWLA IRAVAKQQKE
260 270 280 290 300
SESTQKAEKE VTRMVVVMVF AYCLCWGPYT FFACFATAHP GYAFHPLVAS
310 320 330 340 350
LPSYFAKSAT IYNPIIYVFM NRQFRNCILH LFGKKVDDSS ELSSTSKTEV
SSVSSVSPA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AYN7 | B1AYN7_MOUSE | Green cone photoreceptor pigment | Opn1mw | 389 | Annotation score: | ||
B1AYN8 | B1AYN8_MOUSE | Green cone photoreceptor pigment | Opn1mw | 164 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF011389 mRNA Translation: AAB64302.1 AF190672 mRNA Translation: AAG17991.1 AF191085 , AF191080, AF191081, AF191082, AF191083, AF191084 Genomic DNA Translation: AAG17992.1 BC014826 mRNA Translation: AAH14826.1 |
CCDSi | CCDS30220.1 |
RefSeqi | NP_032132.1, NM_008106.2 |
Genome annotation databases
Ensembli | ENSMUST00000033771; ENSMUSP00000033771; ENSMUSG00000031394 |
GeneIDi | 14539 |
KEGGi | mmu:14539 |
UCSCi | uc009tnv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF011389 mRNA Translation: AAB64302.1 AF190672 mRNA Translation: AAG17991.1 AF191085 , AF191080, AF191081, AF191082, AF191083, AF191084 Genomic DNA Translation: AAG17992.1 BC014826 mRNA Translation: AAH14826.1 |
CCDSi | CCDS30220.1 |
RefSeqi | NP_032132.1, NM_008106.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033771 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | O35599, 1 site |
PhosphoSitePlusi | O35599 |
Proteomic databases
PaxDbi | O35599 |
PRIDEi | O35599 |
Genome annotation databases
Ensembli | ENSMUST00000033771; ENSMUSP00000033771; ENSMUSG00000031394 |
GeneIDi | 14539 |
KEGGi | mmu:14539 |
UCSCi | uc009tnv.1, mouse |
Organism-specific databases
CTDi | 2652 |
MGIi | MGI:1097692, Opn1mw |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00960000186618 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | O35599 |
OMAi | QRWGPQR |
OrthoDBi | 940057at2759 |
PhylomeDBi | O35599 |
TreeFami | TF324998 |
Enzyme and pathway databases
Reactomei | R-MMU-2187335, The retinoid cycle in cones (daylight vision) R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Miscellaneous databases
BioGRID-ORCSi | 14539, 0 hits in 17 CRISPR screens |
PROi | PR:O35599 |
RNActi | O35599, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031394, Expressed in retinal neural layer and 43 other tissues |
ExpressionAtlasi | O35599, baseline and differential |
Genevisiblei | O35599, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR000378, Opsin_red/grn IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00575, OPSINREDGRN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPSG_MOUSE | |
Accessioni | O35599Primary (citable) accession number: O35599 Secondary accession number(s): Q548Z4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families