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Protein

Intraflagellar transport protein 81 homolog

Gene

Ift81

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling.By similarity
Isoform CDV-1: May be involved in cardiac hypertrophy caused by carnitine deficiency.1 Publication
Isoform CDV-1R: May play a role in development of the testis and spermatogenesis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processCilium biogenesis/degradation, Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-MMU-5620924 Intraflagellar transport

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 81 homolog
Alternative name(s):
Carnitine deficiency-associated protein expressed in ventricle 1
Short name:
CDV-1
Gene namesi
Name:Ift81
Synonyms:Cdv-1, Cdv1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1098597 Ift81

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000209182 – 676Intraflagellar transport protein 81 homologAdd BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei61PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35594
MaxQBiO35594
PaxDbiO35594
PRIDEiO35594

PTM databases

iPTMnetiO35594
PhosphoSitePlusiO35594

Expressioni

Tissue specificityi

Isoform CDV-1 is expressed specifically in heart and suppressed specifically in ventricles, but not in auricles, of carnitine-deficient juvenile visceral steatosis (JVS) mice. Isoform CDV-1R is expressed predominantly in the testis. Co-localizes with RABL2/RABL2A in the midpiece of elongated spermatids within the testis (at protein level).3 Publications

Developmental stagei

Isoform CDV-1R is poorly expressed in the testis 15 days after birth. Levels increase sharply between days 15 and 30 reaching a maximum by day 45 and the onset of spermatogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000029469 Expressed in 265 organ(s), highest expression level in testis
ExpressionAtlasiO35594 baseline and differential
GenevisibleiO35594 MM

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT81, and IFT88 (PubMed:19253336). Interacts with IFT74; the interaction is direct: within the IFT complex B, IFT74 and IFT81 mediate the transport of tubulin within the cilium. Interacts with tubulin; the interaction is direct (By similarity). Interacts with RABL2/RABL2A; binding is equal in the presence of GTP or GDP (PubMed:23055941). Interacts with IFT88 (PubMed:19253336). Interacts (via the IFT74/IFT81 heterodimer) with RABL2B (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198662, 2 interactors
STRINGi10090.ENSMUSP00000031426

Structurei

3D structure databases

ProteinModelPortaliO35594
SMRiO35594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 121CH (calponin-homology)-like regionBy similarityAdd BLAST120

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili133 – 258Sequence analysisAdd BLAST126
Coiled coili306 – 388Sequence analysisAdd BLAST83
Coiled coili433 – 453Sequence analysisAdd BLAST21
Coiled coili497 – 628Sequence analysisAdd BLAST132

Domaini

The CH (calponin-homology)-like region shows high similarity to a CH (calponin-homology) domain and mediated binding to the globular domain of tubulin.By similarity

Sequence similaritiesi

Belongs to the IFT81 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHK2 Eukaryota
ENOG410XVNJ LUCA
GeneTreeiENSGT00390000000999
HOGENOMiHOG000237323
HOVERGENiHBG048817
InParanoidiO35594
KOiK19677
OMAiHYTNCMI
OrthoDBiEOG091G05S4
PhylomeDBiO35594
TreeFamiTF314635

Family and domain databases

InterProiView protein in InterPro
IPR029600 IFT81
PANTHERiPTHR15614 PTHR15614, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform CDV-1R (identifier: O35594-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDQIKFIVD SLNKEPFKKN YNLITFDSLG PMQLLQVLND VLAEIDPKQD
60 70 80 90 100
VDIREEMPEQ TAKRMLNLLG ILKYKPPGNA TDMSTFRQGL VIGSKPVIYP
110 120 130 140 150
VLHWLLQRSN ELKKRAYLAR FLIKLEVPSE FLQDETVADT NKQYEELMEA
160 170 180 190 200
FKTLHKECEQ LKTSGFSTAE IRRDISAMEE EKDQLMKRVE RLKKRVETVQ
210 220 230 240 250
NHQRMLKIAR QLRVEKEREE FLAQQKQEQK NQLFHAVQRL QRVQNQLKSM
260 270 280 290 300
RHAAADAKPE SLMKRLEEEI KFNSYMVTEK FPKELESKKK ELHFLQKVVS
310 320 330 340 350
EPAMGHSDLL ELETKVNEVN TEINQLIEKK MMRNEPIEGK LSLYRQQASI
360 370 380 390 400
ISRKKEAKAE ELQETKEKLA SLEREVLVKT NQTREFDGTE VLKGDEFKRY
410 420 430 440 450
VSKLRSKSTV FKKKHQIIAE FKAEFGLLQR TEELLKQRQE TIQHQLRTIE
460 470 480 490 500
EKKGISGYSY TQEELERVSA LKSEVDEMKG RTLDDMSEMV KKLNSLVSEK
510 520 530 540 550
KSALAPVIKE LRQLRQKCQE LTQECDEKKA QYDSCAAGLE SNRSKLEQEV
560 570 580 590 600
RGLREECLQE ESKYHYTNCM IKNLEVELRR ATDEMKAYVS SDQQEKRKAI
610 620 630 640 650
REQYTKNITE QENLGKKLRE KQKAVRESHG PNMKQAKMWR DLEQLMECKK
660 670
QCFLKQQSPA SIGQVIQEGG EDRLVL
Length:676
Mass (Da):79,285
Last modified:March 29, 2004 - v4
Checksum:iDB059ACCFFDEBAE8
GO
Isoform CDV-1 (identifier: O35594-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-569: Missing.

Show »
Length:107
Mass (Da):12,578
Checksum:i624537E705E6E514
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z5A8D3Z5A8_MOUSE
Intraflagellar transport protein 81...
Ift81
217Annotation score:
A0A0G2JFG8A0A0G2JFG8_MOUSE
Intraflagellar transport protein 81...
Ift81
198Annotation score:
D6RHM7D6RHM7_MOUSE
Intraflagellar transport protein 81...
Ift81
59Annotation score:
A0A0G2JGC3A0A0G2JGC3_MOUSE
Intraflagellar transport protein 81...
Ift81
64Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187851 – 569Missing in isoform CDV-1. 2 PublicationsAdd BLAST569

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10495 mRNA Translation: CAA71519.2
Y10496 mRNA Translation: CAA71520.1
AF203441
, AF203431, AF203432, AF203433, AF203434, AF203435, AF203436, AF203437, AF203438, AF203439, AF203440 Genomic DNA Translation: AAG35650.1
AF203441
, AF203438, AF203439, AF203440 Genomic DNA Translation: AAG35651.1
AF354757 mRNA Translation: AAL83977.1
AK013424 mRNA Translation: BAB28849.3
AK042372 mRNA Translation: BAC31242.1
BC055721 mRNA Translation: AAH55721.1
CCDSiCCDS19650.1 [O35594-1]
RefSeqiNP_034009.2, NM_009879.3 [O35594-1]
XP_006530190.1, XM_006530127.3
UniGeneiMm.10125

Genome annotation databases

EnsembliENSMUST00000031426; ENSMUSP00000031426; ENSMUSG00000029469 [O35594-1]
GeneIDi12589
KEGGimmu:12589
UCSCiuc008zlm.2 mouse [O35594-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10495 mRNA Translation: CAA71519.2
Y10496 mRNA Translation: CAA71520.1
AF203441
, AF203431, AF203432, AF203433, AF203434, AF203435, AF203436, AF203437, AF203438, AF203439, AF203440 Genomic DNA Translation: AAG35650.1
AF203441
, AF203438, AF203439, AF203440 Genomic DNA Translation: AAG35651.1
AF354757 mRNA Translation: AAL83977.1
AK013424 mRNA Translation: BAB28849.3
AK042372 mRNA Translation: BAC31242.1
BC055721 mRNA Translation: AAH55721.1
CCDSiCCDS19650.1 [O35594-1]
RefSeqiNP_034009.2, NM_009879.3 [O35594-1]
XP_006530190.1, XM_006530127.3
UniGeneiMm.10125

3D structure databases

ProteinModelPortaliO35594
SMRiO35594
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198662, 2 interactors
STRINGi10090.ENSMUSP00000031426

PTM databases

iPTMnetiO35594
PhosphoSitePlusiO35594

Proteomic databases

EPDiO35594
MaxQBiO35594
PaxDbiO35594
PRIDEiO35594

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031426; ENSMUSP00000031426; ENSMUSG00000029469 [O35594-1]
GeneIDi12589
KEGGimmu:12589
UCSCiuc008zlm.2 mouse [O35594-1]

Organism-specific databases

CTDi28981
MGIiMGI:1098597 Ift81

Phylogenomic databases

eggNOGiENOG410IHK2 Eukaryota
ENOG410XVNJ LUCA
GeneTreeiENSGT00390000000999
HOGENOMiHOG000237323
HOVERGENiHBG048817
InParanoidiO35594
KOiK19677
OMAiHYTNCMI
OrthoDBiEOG091G05S4
PhylomeDBiO35594
TreeFamiTF314635

Enzyme and pathway databases

ReactomeiR-MMU-5620924 Intraflagellar transport

Miscellaneous databases

PROiPR:O35594
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029469 Expressed in 265 organ(s), highest expression level in testis
ExpressionAtlasiO35594 baseline and differential
GenevisibleiO35594 MM

Family and domain databases

InterProiView protein in InterPro
IPR029600 IFT81
PANTHERiPTHR15614 PTHR15614, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIFT81_MOUSE
AccessioniPrimary (citable) accession number: O35594
Secondary accession number(s): Q8R4W2, Q9CSA1, Q9EQM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 29, 2004
Last modified: November 7, 2018
This is version 132 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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