UniProtKB - O35593 (PSDE_MOUSE)
Protein
26S proteasome non-ATPase regulatory subunit 14
Gene
Psmd14
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 113 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 115 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 126 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- endopeptidase activator activity Source: MGI
- endopeptidase activity Source: MGI
- isopeptidase activity Source: InterPro
- Lys63-specific deubiquitinase activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- metallopeptidase activity Source: GO_Central
- proteasome binding Source: MGI
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
GO - Biological processi
- double-strand break repair via homologous recombination Source: UniProtKB
- double-strand break repair via nonhomologous end joining Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein deubiquitination Source: GO_Central
- protein K63-linked deubiquitination Source: UniProtKB
- regulation of proteasomal protein catabolic process Source: MGI
- response to ethanol Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | DNA damage, DNA repair, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-5689901, Metalloprotease DUBs R-MMU-6798695, Neutrophil degranulation R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | M67.001 |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome non-ATPase regulatory subunit 14 (EC:3.4.19.-)Alternative name(s): 26S proteasome regulatory subunit RPN11 MAD1 |
Gene namesi | Name:Psmd14 Synonyms:Pad1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913284, Psmd14 |
Subcellular locationi
Cytosol
- cytosolic proteasome complex Source: MGI
Other locations
- proteasome accessory complex Source: UniProtKB
- proteasome complex Source: MGI
- proteasome regulatory particle, lid subcomplex Source: UniProtKB
Keywords - Cellular componenti
ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213953 | 1 – 310 | 26S proteasome non-ATPase regulatory subunit 14Add BLAST | 310 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 150 | PhosphoserineBy similarity | 1 | |
Modified residuei | 224 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O35593 |
jPOSTi | O35593 |
MaxQBi | O35593 |
PaxDbi | O35593 |
PeptideAtlasi | O35593 |
PRIDEi | O35593 |
TopDownProteomicsi | O35593 |
2D gel databases
REPRODUCTION-2DPAGEi | O35593 |
PTM databases
iPTMneti | O35593 |
PhosphoSitePlusi | O35593 |
SwissPalmi | O35593 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026914, Expressed in olfactory bulb and 312 other tissues |
Genevisiblei | O35593, MM |
Interactioni
Subunit structurei
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD4, a base containing 6 ATPases and few additional components. Within the complex, PSMD4 interacts with subunit PSMD7 through their respective MPN domain.
Interacts with TXNL1.
By similarityBinary interactionsi
O35593
With | #Exp. | IntAct |
---|---|---|
ADRM1 [Q16186] from Homo sapiens. | 2 | EBI-772796,EBI-954387 |
Protein-protein interaction databases
BioGRIDi | 208496, 61 interactors |
IntActi | O35593, 45 interactors |
MINTi | O35593 |
STRINGi | 10090.ENSMUSP00000028278 |
Miscellaneous databases
RNActi | O35593, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 166 | MPNPROSITE-ProRule annotationAdd BLAST | 136 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 113 – 126 | JAMM motifPROSITE-ProRule annotationAdd BLAST | 14 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1555, Eukaryota |
GeneTreei | ENSGT00730000111116 |
HOGENOMi | CLU_052991_0_1_1 |
InParanoidi | O35593 |
OMAi | KTGRHEM |
OrthoDBi | 1031881at2759 |
PhylomeDBi | O35593 |
TreeFami | TF105748 |
Family and domain databases
InterProi | View protein in InterPro IPR000555, JAMM/MPN+_dom IPR037518, MPN IPR035299, PSMD14 IPR024969, Rpn11/EIF3F_C |
PANTHERi | PTHR10410:SF22, PTHR10410:SF22, 1 hit |
Pfami | View protein in Pfam PF01398, JAB, 1 hit PF13012, MitMem_reg, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
i Sequence
Sequence statusi: Complete.
O35593-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP
60 70 80 90 100
MEVMGLMLGE FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK
110 120 130 140 150
QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS
160 170 180 190 200
VKGKVVIDAF RLINANMMVL GHEPRQTTSN LGHLNKPSIQ ALIHGLNRHY
210 220 230 240 250
YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH NESVVKEMLE
260 270 280 290 300
LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL
310
AAMLDTVVFK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 – 17 | GQG → AR in CAA73514 (PubMed:9727008).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13071 mRNA Translation: CAA73514.1 AK012013 mRNA Translation: BAB27974.1 AK151104 mRNA Translation: BAE30114.1 AK151733 mRNA Translation: BAE30648.1 BC003742 mRNA Translation: AAH03742.1 |
CCDSi | CCDS38127.1 |
RefSeqi | NP_067501.2, NM_021526.2 |
Genome annotation databases
Ensembli | ENSMUST00000028278; ENSMUSP00000028278; ENSMUSG00000026914 |
GeneIDi | 59029 |
KEGGi | mmu:59029 |
UCSCi | uc008jvc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13071 mRNA Translation: CAA73514.1 AK012013 mRNA Translation: BAB27974.1 AK151104 mRNA Translation: BAE30114.1 AK151733 mRNA Translation: BAE30648.1 BC003742 mRNA Translation: AAH03742.1 |
CCDSi | CCDS38127.1 |
RefSeqi | NP_067501.2, NM_021526.2 |
3D structure databases
SMRi | O35593 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208496, 61 interactors |
IntActi | O35593, 45 interactors |
MINTi | O35593 |
STRINGi | 10090.ENSMUSP00000028278 |
Protein family/group databases
MEROPSi | M67.001 |
PTM databases
iPTMneti | O35593 |
PhosphoSitePlusi | O35593 |
SwissPalmi | O35593 |
2D gel databases
REPRODUCTION-2DPAGEi | O35593 |
Proteomic databases
EPDi | O35593 |
jPOSTi | O35593 |
MaxQBi | O35593 |
PaxDbi | O35593 |
PeptideAtlasi | O35593 |
PRIDEi | O35593 |
TopDownProteomicsi | O35593 |
Protocols and materials databases
Antibodypediai | 1047, 239 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028278; ENSMUSP00000028278; ENSMUSG00000026914 |
GeneIDi | 59029 |
KEGGi | mmu:59029 |
UCSCi | uc008jvc.1, mouse |
Organism-specific databases
CTDi | 10213 |
MGIi | MGI:1913284, Psmd14 |
Phylogenomic databases
eggNOGi | KOG1555, Eukaryota |
GeneTreei | ENSGT00730000111116 |
HOGENOMi | CLU_052991_0_1_1 |
InParanoidi | O35593 |
OMAi | KTGRHEM |
OrthoDBi | 1031881at2759 |
PhylomeDBi | O35593 |
TreeFami | TF105748 |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-5689901, Metalloprotease DUBs R-MMU-6798695, Neutrophil degranulation R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 59029, 10 hits in 17 CRISPR screens |
ChiTaRSi | Psmd14, mouse |
PROi | PR:O35593 |
RNActi | O35593, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026914, Expressed in olfactory bulb and 312 other tissues |
Genevisiblei | O35593, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000555, JAMM/MPN+_dom IPR037518, MPN IPR035299, PSMD14 IPR024969, Rpn11/EIF3F_C |
PANTHERi | PTHR10410:SF22, PTHR10410:SF22, 1 hit |
Pfami | View protein in Pfam PF01398, JAB, 1 hit PF13012, MitMem_reg, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSDE_MOUSE | |
Accessioni | O35593Primary (citable) accession number: O35593 Secondary accession number(s): Q3UB50, Q9CZY6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | August 31, 2004 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families