Pfkfb3

Functionⁱ

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityⁱ

β-D-fructose 2,6-bisphosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
H₂O
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
β-D-fructose 6-phosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
phosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
EC:
3.1.3.46
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
β-D-fructose 2,6-bisphosphate
zoom
+
H₂O
=
β-D-fructose 6-phosphate
zoom
+
phosphate
zoom
ATP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
β-D-fructose 6-phosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
ADP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
β-D-fructose 2,6-bisphosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
EC:
2.7.1.105
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
ATP
+
β-D-fructose 6-phosphate
zoom
=
ADP
+
β-D-fructose 2,6-bisphosphate
zoom
+
H⁺

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	75	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	99	Fructose 6-phosphateBy similarity	1
Active siteⁱ	125	Sequence analysis	1
Binding siteⁱ	127	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	133	Fructose 6-phosphateBy similarity	1
Active siteⁱ	155	Sequence analysis	1
Binding siteⁱ	169	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	190	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	194	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	253	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	253	Transition state stabilizerBy similarity	1
Active siteⁱ	254	Tele-phosphohistidine intermediateBy similarity	1
Binding siteⁱ	260	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	260	Transition state stabilizerBy similarity	1
Binding siteⁱ	266	Fructose 2,6-bisphosphate; via amide nitrogenBy similarity	1
Active siteⁱ	323	Proton donor/acceptorBy similarity	1
Binding siteⁱ	334	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	348	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	352	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	363	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	388	Transition state stabilizerBy similarity	1
Binding siteⁱ	389	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	393	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	425	ATPBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	42 – 50	ATPBy similarity	9
Nucleotide bindingⁱ	164 – 169	ATPBy similarity	6
Nucleotide bindingⁱ	345 – 348	ATPBy similarity	4
Nucleotide bindingⁱ	389 – 393	ATPBy similarity	5

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Kinase, Multifunctional enzyme, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	3.1.3.46, 5301
Reactomeⁱ	R-RNO-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Short name: 6PF-2-K/Fru-2,6-P2ase 3 Short name: PFK/FBPase 3 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase brain-type isozyme RB2K Including the following 2 domains: 6-phosphofructo-2-kinase (EC:2.7.1.105) Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesⁱ	Name:Pfkfb3
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 17

Organism-specific databases

Rat genome database More...RGDⁱ	619776, Pfkfb3

RGDⁱ

619776, Pfkfb3

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000179969	1 – 555	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Add BLAST		555

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	490	Phosphoserine; by AMPK and PKABy similarity	1
Modified residueⁱ	492	PhosphothreonineBy similarity	1
Modified residueⁱ	496	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylation by AMPK stimulates activity.By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

jPOSTⁱ	O35552
PaxDbⁱ	O35552

PTM databases

iPTMnetⁱ	O35552
PhosphoSitePlusⁱ	O35552

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000018911, Expressed in skeletal muscle tissue and 20 other tissues
ExpressionAtlasⁱ	O35552, baseline and differential
Genevisibleⁱ	O35552, RN

Interactionⁱ

Protein-protein interaction databases

ComplexPortalⁱ	CPX-2043, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 complex
IntActⁱ	O35552, 2 interactors
STRINGⁱ	10116.ENSRNOP00000062965

Structureⁱ

3D structure databases

SMRⁱ	O35552
ModBaseⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 245	6-phosphofructo-2-kinaseAdd BLAST		245
Regionⁱ	246 – 555	Fructose-2,6-bisphosphataseAdd BLAST		310

Sequence similaritiesⁱ

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0234, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00950000182835
HOGENOMⁱ	CLU_006383_1_1_1
InParanoidⁱ	O35552
OMAⁱ	DMLAWFR
Database of Orthologous Groups More...OrthoDBⁱ	392001at2759
PhylomeDBⁱ	O35552
TreeFamⁱ	TF313541

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07067, HP_PGM_like, 1 hit
Gene3Dⁱ	3.40.50.1240, 1 hit
InterProⁱ	View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS
PANTHERⁱ	PTHR10606, PTHR10606, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit
PRINTSⁱ	PR00991, 6PFRUCTKNASE
SMARTⁱ	View protein in SMART SM00855, PGAM, 1 hit
SUPFAMⁱ	SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit
PROSITEⁱ	View protein in PROSITE PS00175, PG_MUTASE, 1 hit

Sequences (8+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 8 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 8 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: O35552-1) [UniParc]FASTA Add to basket

Also known as: RB2K1

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 
       460        470        480        490        500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 
       510        520        530        540        550
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS 

KFSPY

Length:555

Mass (Da):63,676

Last modified:January 1, 1998 - v1

Checksum:ⁱ45B2D090B44FCD8D

GO

Isoform 2 (identifier: O35552-2) [UniParc]FASTA Add to basket

Also known as: RB2K2

The sequence of this isoform differs from the canonical sequence as follows:
543-555: RTVCHIFSKFSPY → T

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 
       460        470        480        490        500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 
       510        520        530        540 
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLT

Show »

Length:543

Mass (Da):62,210

Checksum:ⁱDA1B3F86851815D1

GO

Isoform 3 (identifier: O35552-3) [UniParc]FASTA Add to basket

Also known as: RB2K3

The sequence of this isoform differs from the canonical sequence as follows:
535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 
       460        470        480        490        500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 
       510        520        530        540 
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQNMRSPR SGAESSQKH

Show »

Length:549

Mass (Da):62,967

Checksum:ⁱ26E9DB0564825A82

GO

Isoform 4 (identifier: O35552-4) [UniParc]FASTA Add to basket

Also known as: RB2K4

The sequence of this isoform differs from the canonical sequence as follows:
448-476: Missing.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEDAK 
       460        470        480        490        500
KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 
       510        520 
QLPGQPLLGK ACLRTVCHIF SKFSPY

Show »

Length:526

Mass (Da):60,456

Checksum:ⁱB41B90799C6003C9

GO

Isoform 5 (identifier: O35552-5) [UniParc]FASTA Add to basket

Also known as: RB2K5

The sequence of this isoform differs from the canonical sequence as follows:
448-476: Missing.
543-555: RTVCHIFSKFSPY → T

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEDAK 
       460        470        480        490        500
KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 
       510 
QLPGQPLLGK ACLT

Show »

Length:514

Mass (Da):58,991

Checksum:ⁱ6D3458ECED6716FC

GO

Isoform 6 (identifier: O35552-6) [UniParc]FASTA Add to basket

Also known as: RB2K6

The sequence of this isoform differs from the canonical sequence as follows:
448-476: Missing.
535-555: PLLGKACLRTVCHIFSKFSPY → NMRSPRSGAESSQKH

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEDAK 
       460        470        480        490        500
KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 
       510        520
QLPGQNMRSP RSGAESSQKH

Show »

Length:520

Mass (Da):59,747

Checksum:ⁱ78AD6CCDF66E1DAD

GO

Isoform 7 (identifier: O35552-7) [UniParc]FASTA Add to basket

Also known as: RB2K7

The sequence of this isoform differs from the canonical sequence as follows:
543-555: Missing.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK 
       460        470        480        490        500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT 
       510        520        530        540 
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CL

Show »

Length:542

Mass (Da):62,109

Checksum:ⁱCB3F86851815D1D7

GO

Isoform 8 (identifier: O35552-8) [UniParc]FASTA Add to basket

Also known as: RB2K8

The sequence of this isoform differs from the canonical sequence as follows:
448-476: Missing.
543-555: Missing.

« Hide

        10         20         30         40         50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY 
        60         70         80         90        100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK 
       110        120        130        140        150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF 
       160        170        180        190        200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP 
       210        220        230        240        250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY 
       260        270        280        290        300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ 
       310        320        330        340        350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ 
       360        370        380        390        400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL 
       410        420        430        440        450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEDAK 
       460        470        480        490        500
KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 
       510 
QLPGQPLLGK ACL

Show »

Length:513

Mass (Da):58,889

Checksum:ⁱ8458ECED6716FC15

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A7UAK3	A7UAK3_RAT		6-phosphofructo-2-kinase 6-phosphofructo-2-kinase, EC 2.7.1.105, EC 3.1.3.46 (Fructose-2,6-bisphosphatase)	Pfkfb3	462	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	185	D → H in BAA22048 (Ref. 2) Curated		1
Sequence conflictⁱ	367	V → L in BAA22048 (Ref. 2) Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_004681	448 – 476	Missing in isoform 4, isoform 5, isoform 6 and isoform 8. CuratedAdd BLAST	29
Alternative sequenceⁱVSP_004683	535 – 555	PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6. CuratedAdd BLAST	21
Alternative sequenceⁱVSP_004682	543 – 555	RTVCH…KFSPY → T in isoform 2 and isoform 5. CuratedAdd BLAST	13
Alternative sequenceⁱVSP_004684	543 – 555	Missing in isoform 7 and isoform 8. CuratedAdd BLAST	13

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D87240 mRNA Translation: BAA21749.1 D87241 mRNA Translation: BAA21750.1 D87242 mRNA Translation: BAA21751.1 D87243 mRNA Translation: BAA21752.1 D87244 mRNA Translation: BAA21753.1 D87245 mRNA Translation: BAA21754.1 D87246 mRNA Translation: BAA21755.1 D87247 mRNA Translation: BAA21756.1 AB006710 mRNA Translation: BAA22048.1
NCBI Reference Sequences More...RefSeqⁱ	NP_476476.1, NM_057135.1 [O35552-1] XP_006254254.1, XM_006254192.3 [O35552-3] XP_006254255.1, XM_006254193.3 [O35552-2] XP_006254258.1, XM_006254196.3 [O35552-4] XP_006254259.1, XM_006254197.3 [O35552-6] XP_006254260.1, XM_006254198.3 [O35552-5]

Genome annotation databases

Ensemblⁱ	ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911 [O35552-8] ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911 [O35552-2] ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911 [O35552-1]
GeneIDⁱ	117276
KEGGⁱ	rno:117276

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O35552	Isoform 7 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		RAT		542	UniRef90_O35552
6-phosphofructo-2-kinase		MOUSE		555
6-phosphofructo-2-kinase		MUSCR		555
6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3		RHIFE		513
6-phosphofructo-2-kinase		MOUSE		542
+49
O35552-4	Isoform 8 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		RAT		513	UniRef90_O35552-4
6-phosphofructo-2-kinase		RABIT		653
6-phosphofructo-2-kinase		PERMB		548
6-phosphofructo-2-kinase		ICTTR		544
6-phosphofructo-2-kinase		LOXAF		528
+45
O35552-5	Isoform 6 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		RAT		520	UniRef90_O35552-6
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		PONAB		514
6-phosphofructo-2-kinase		SAIBB		548
6-phosphofructo-2-kinase		PROCO		544
6-phosphofructo-2-kinase		PERMB		522
+228

Protein	Similar proteins		Species	Score	Length	Source
O35552	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3	)	HUMAN		520	UniRef50_Q16875
6-phosphofructo-2-kinase		PANTR		520
6-phosphofructo-2-kinase		PONAB		520
6-phosphofructo-2-kinase		PANPA		520
6-phosphofructo-2-kinase		GORGO		520
+454
O35552-5	Isoform 6 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		RAT		520	UniRef50_O35552-6
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		PONAB		514
6-phosphofructo-2-kinase		SAIBB		548
6-phosphofructo-2-kinase		PROCO		544
6-phosphofructo-2-kinase		PERMB		522
+1278

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D87240 mRNA Translation: BAA21749.1 D87241 mRNA Translation: BAA21750.1 D87242 mRNA Translation: BAA21751.1 D87243 mRNA Translation: BAA21752.1 D87244 mRNA Translation: BAA21753.1 D87245 mRNA Translation: BAA21754.1 D87246 mRNA Translation: BAA21755.1 D87247 mRNA Translation: BAA21756.1 AB006710 mRNA Translation: BAA22048.1
RefSeqⁱ	NP_476476.1, NM_057135.1 [O35552-1] XP_006254254.1, XM_006254192.3 [O35552-3] XP_006254255.1, XM_006254193.3 [O35552-2] XP_006254258.1, XM_006254196.3 [O35552-4] XP_006254259.1, XM_006254197.3 [O35552-6] XP_006254260.1, XM_006254198.3 [O35552-5]

3D structure databases

SMRⁱ	O35552
ModBaseⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-2043, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 complex
IntActⁱ	O35552, 2 interactors
STRINGⁱ	10116.ENSRNOP00000062965

PTM databases

iPTMnetⁱ	O35552
PhosphoSitePlusⁱ	O35552

Proteomic databases

jPOSTⁱ	O35552
PaxDbⁱ	O35552

Genome annotation databases

Ensemblⁱ	ENSRNOT00000025731; ENSRNOP00000025731; ENSRNOG00000018911 [O35552-8] ENSRNOT00000051067; ENSRNOP00000040928; ENSRNOG00000018911 [O35552-2] ENSRNOT00000068354; ENSRNOP00000062965; ENSRNOG00000018911 [O35552-1]
GeneIDⁱ	117276
KEGGⁱ	rno:117276

Organism-specific databases

CTDⁱ	5209
RGDⁱ	619776, Pfkfb3

Phylogenomic databases

eggNOGⁱ	KOG0234, Eukaryota
GeneTreeⁱ	ENSGT00950000182835
HOGENOMⁱ	CLU_006383_1_1_1
InParanoidⁱ	O35552
OMAⁱ	DMLAWFR
OrthoDBⁱ	392001at2759
PhylomeDBⁱ	O35552
TreeFamⁱ	TF313541

Enzyme and pathway databases

BRENDAⁱ	3.1.3.46, 5301
Reactomeⁱ	R-RNO-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O35552

PROⁱ

PR:O35552

Gene expression databases

Bgeeⁱ	ENSRNOG00000018911, Expressed in skeletal muscle tissue and 20 other tissues
ExpressionAtlasⁱ	O35552, baseline and differential
Genevisibleⁱ	O35552, RN

Family and domain databases

CDDⁱ	cd07067, HP_PGM_like, 1 hit
Gene3Dⁱ	3.40.50.1240, 1 hit
InterProⁱ	View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS
PANTHERⁱ	PTHR10606, PTHR10606, 1 hit
Pfamⁱ	View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit
PRINTSⁱ	PR00991, 6PFRUCTKNASE
SMARTⁱ	View protein in SMART SM00855, PGAM, 1 hit
SUPFAMⁱ	SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit
PROSITEⁱ	View protein in PROSITE PS00175, PG_MUTASE, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	F263_RAT
Accessionⁱ	O35552Primary (citable) accession number: O35552 Secondary accession number(s): O35096 Q9QWQ6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
	Last sequence update:	January 1, 1998
	Last modified:	December 2, 2020
	This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O35552 (F263_RAT)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ