UniProtKB - O35552 (F263_RAT)
Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Gene
Pfkfb3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 99 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 125 | Sequence analysis | 1 | |
Binding sitei | 127 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 133 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 155 | Sequence analysis | 1 | |
Binding sitei | 169 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 190 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 194 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 253 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 253 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 254 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 260 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 260 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 266 | Fructose 2,6-bisphosphate; via amide nitrogenBy similarity | 1 | |
Active sitei | 323 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 334 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 348 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 352 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 363 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 388 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 389 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 393 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 425 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 42 – 50 | ATPBy similarity | 9 | |
Nucleotide bindingi | 164 – 169 | ATPBy similarity | 6 | |
Nucleotide bindingi | 345 – 348 | ATPBy similarity | 4 | |
Nucleotide bindingi | 389 – 393 | ATPBy similarity | 5 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- fructose-2,6-bisphosphate 2-phosphatase activity Source: GO_Central
GO - Biological processi
- brain development Source: RGD
- fructose 2,6-bisphosphate metabolic process Source: RGD
- fructose metabolic process Source: InterPro
- regulation of glycolytic process Source: RGD
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 5301 |
Reactomei | R-RNO-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Short name: 6PF-2-K/Fru-2,6-P2ase 3 Short name: PFK/FBPase 3 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase brain-type isozyme RB2K Including the following 2 domains: Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
Gene namesi | Name:Pfkfb3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619776, Pfkfb3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000179969 | 1 – 555 | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Add BLAST | 555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 490 | Phosphoserine; by AMPK and PKABy similarity | 1 | |
Modified residuei | 492 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 496 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation by AMPK stimulates activity.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O35552 |
PaxDbi | O35552 |
PTM databases
iPTMneti | O35552 |
PhosphoSitePlusi | O35552 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018911, Expressed in skeletal muscle tissue and 20 other tissues |
ExpressionAtlasi | O35552, baseline and differential |
Genevisiblei | O35552, RN |
Interactioni
Protein-protein interaction databases
ComplexPortali | CPX-2043, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 complex |
IntActi | O35552, 2 interactors |
STRINGi | 10116.ENSRNOP00000062965 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 245 | 6-phosphofructo-2-kinaseAdd BLAST | 245 | |
Regioni | 246 – 555 | Fructose-2,6-bisphosphataseAdd BLAST | 310 |
Sequence similaritiesi
In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_1_1_1 |
InParanoidi | O35552 |
OMAi | DMLAWFR |
OrthoDBi | 392001at2759 |
PhylomeDBi | O35552 |
TreeFami | TF313541 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
s (8+)i Sequence
Sequence statusi: Complete.
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O35552-1) [UniParc]FASTAAdd to basket
Also known as: RB2K1
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY
60 70 80 90 100
ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK
110 120 130 140 150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF
160 170 180 190 200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP
210 220 230 240 250
LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY
260 270 280 290 300
LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ
310 320 330 340 350
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ
360 370 380 390 400
DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL
410 420 430 440 450
DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK
460 470 480 490 500
IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT
510 520 530 540 550
KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS
KFSPY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA7UAK3 | A7UAK3_RAT | 6-phosphofructo-2-kinase | Pfkfb3 | 462 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 185 | D → H in BAA22048 (Ref. 2) Curated | 1 | |
Sequence conflicti | 367 | V → L in BAA22048 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004681 | 448 – 476 | Missing in isoform 4, isoform 5, isoform 6 and isoform 8. CuratedAdd BLAST | 29 | |
Alternative sequenceiVSP_004683 | 535 – 555 | PLLGK…KFSPY → NMRSPRSGAESSQKH in isoform 3 and isoform 6. CuratedAdd BLAST | 21 | |
Alternative sequenceiVSP_004682 | 543 – 555 | RTVCH…KFSPY → T in isoform 2 and isoform 5. CuratedAdd BLAST | 13 | |
Alternative sequenceiVSP_004684 | 543 – 555 | Missing in isoform 7 and isoform 8. CuratedAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87240 mRNA Translation: BAA21749.1 D87241 mRNA Translation: BAA21750.1 D87242 mRNA Translation: BAA21751.1 D87243 mRNA Translation: BAA21752.1 D87244 mRNA Translation: BAA21753.1 D87245 mRNA Translation: BAA21754.1 D87246 mRNA Translation: BAA21755.1 D87247 mRNA Translation: BAA21756.1 AB006710 mRNA Translation: BAA22048.1 |
RefSeqi | NP_476476.1, NM_057135.1 [O35552-1] XP_006254254.1, XM_006254192.3 [O35552-3] XP_006254255.1, XM_006254193.3 [O35552-2] XP_006254258.1, XM_006254196.3 [O35552-4] XP_006254259.1, XM_006254197.3 [O35552-6] XP_006254260.1, XM_006254198.3 [O35552-5] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87240 mRNA Translation: BAA21749.1 D87241 mRNA Translation: BAA21750.1 D87242 mRNA Translation: BAA21751.1 D87243 mRNA Translation: BAA21752.1 D87244 mRNA Translation: BAA21753.1 D87245 mRNA Translation: BAA21754.1 D87246 mRNA Translation: BAA21755.1 D87247 mRNA Translation: BAA21756.1 AB006710 mRNA Translation: BAA22048.1 |
RefSeqi | NP_476476.1, NM_057135.1 [O35552-1] XP_006254254.1, XM_006254192.3 [O35552-3] XP_006254255.1, XM_006254193.3 [O35552-2] XP_006254258.1, XM_006254196.3 [O35552-4] XP_006254259.1, XM_006254197.3 [O35552-6] XP_006254260.1, XM_006254198.3 [O35552-5] |
3D structure databases
SMRi | O35552 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-2043, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 complex |
IntActi | O35552, 2 interactors |
STRINGi | 10116.ENSRNOP00000062965 |
PTM databases
iPTMneti | O35552 |
PhosphoSitePlusi | O35552 |
Proteomic databases
jPOSTi | O35552 |
PaxDbi | O35552 |
Genome annotation databases
Organism-specific databases
CTDi | 5209 |
RGDi | 619776, Pfkfb3 |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_1_1_1 |
InParanoidi | O35552 |
OMAi | DMLAWFR |
OrthoDBi | 392001at2759 |
PhylomeDBi | O35552 |
TreeFami | TF313541 |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 5301 |
Reactomei | R-RNO-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism |
Miscellaneous databases
PROi | PR:O35552 |
Gene expression databases
Bgeei | ENSRNOG00000018911, Expressed in skeletal muscle tissue and 20 other tissues |
ExpressionAtlasi | O35552, baseline and differential |
Genevisiblei | O35552, RN |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F263_RAT | |
Accessioni | O35552Primary (citable) accession number: O35552 Secondary accession number(s): O35096 Q9QWQ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families