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UniProtKB - O35532 (MSMO1_RAT)
Protein
Methylsterol monooxygenase 1
Gene
Msmo1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol.
2 PublicationsCatalytic activityi
- 4,4-dimethyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-7-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2O1 PublicationEC:1.14.18.91 PublicationThis reaction proceeds in the forward1 Publication direction.
- 4,4-dimethyl-5α-cholesta-8,24-dien-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4β-methylzymosterol-4α-carboxylate + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methylzymosterol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxyzymosterol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-5α-cholest-7-en-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 4,4-dimethyl-5α-cholest-8-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-8-en-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methyl-5α-cholest-8-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-5α-cholest-8-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Fe cationBy similarity
: zymosterol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.Curated This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.
Pathwayi: cholesterol biosynthesis
This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.2 PublicationsView all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.
GO - Molecular functioni
- C-4 methylsterol oxidase activity Source: GO_Central
- iron ion binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- cholesterol biosynthetic process Source: UniProtKB-UniPathway
- sterol biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Iron, NAD |
Enzyme and pathway databases
Reactomei | R-RNO-191273, Cholesterol biosynthesis |
SABIO-RKi | O35532 |
UniPathwayi | UPA00063 UPA00770;UER00756 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylsterol monooxygenase 1 (EC:1.14.18.91 Publication)Alternative name(s): C-4 methylsterol oxidase Neuropep 1 RANP-1 Sterol-C4-methyl oxidase |
Gene namesi | Name:Msmo1 Synonyms:Sc4mol |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620281, Msmo1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 100 – 120 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 199 – 219 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000117036 | 1 – 293 | Methylsterol monooxygenase 1Add BLAST | 293 |
Proteomic databases
jPOSTi | O35532 |
PaxDbi | O35532 |
PRIDEi | O35532 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000032297, Expressed in female gonad and 22 other tissues |
Genevisiblei | O35532, RN |
Interactioni
Protein-protein interaction databases
IntActi | O35532, 4 interactors |
STRINGi | 10116.ENSRNOP00000043782 |
Structurei
3D structure databases
AlphaFoldDBi | O35532 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 144 – 274 | Fatty acid hydroxylaseSequence analysisAdd BLAST | 131 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 157 – 161 | Histidine box-1 | 5 | |
Motifi | 170 – 174 | Histidine box-2 | 5 | |
Motifi | 249 – 255 | Histidine box-3 | 7 |
Domaini
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similaritiesi
Belongs to the sterol desaturase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0873, Eukaryota |
GeneTreei | ENSGT00940000158012 |
HOGENOMi | CLU_047036_5_2_1 |
InParanoidi | O35532 |
OMAi | IVHEFIY |
OrthoDBi | 1493916at2759 |
PhylomeDBi | O35532 |
TreeFami | TF354294 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
i Sequence
Sequence statusi: Complete.
O35532-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMNKSVGLF SSASLAVDYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA
60 70 80 90 100
TWGSLIVHET IYFLFSLPGF LFQFIPFMRK YKIQKDKPET FEGQWKCLKG
110 120 130 140 150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYFTLA RCLGCAVIED
160 170 180 190 200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF
210 220 230 240 250
IGIVLLCDHV ILLWAWVTMR LLETIDVHSG YDIPLNPLNY IPFYTGARHH
260 270 280 290
DFHHMNFIGN YASTFTWWDR IFGTDVQYHA YTEKMKKLGK KSE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50559 mRNA Translation: BAA23329.1 BC063155 mRNA Translation: AAH63155.1 |
RefSeqi | NP_543162.1, NM_080886.1 |
Genome annotation databases
Ensembli | ENSRNOT00000044171; ENSRNOP00000043782; ENSRNOG00000032297 |
GeneIDi | 140910 |
KEGGi | rno:140910 |
UCSCi | RGD:620281, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50559 mRNA Translation: BAA23329.1 BC063155 mRNA Translation: AAH63155.1 |
RefSeqi | NP_543162.1, NM_080886.1 |
3D structure databases
AlphaFoldDBi | O35532 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | O35532, 4 interactors |
STRINGi | 10116.ENSRNOP00000043782 |
Proteomic databases
jPOSTi | O35532 |
PaxDbi | O35532 |
PRIDEi | O35532 |
Genome annotation databases
Ensembli | ENSRNOT00000044171; ENSRNOP00000043782; ENSRNOG00000032297 |
GeneIDi | 140910 |
KEGGi | rno:140910 |
UCSCi | RGD:620281, rat |
Organism-specific databases
CTDi | 6307 |
RGDi | 620281, Msmo1 |
Phylogenomic databases
eggNOGi | KOG0873, Eukaryota |
GeneTreei | ENSGT00940000158012 |
HOGENOMi | CLU_047036_5_2_1 |
InParanoidi | O35532 |
OMAi | IVHEFIY |
OrthoDBi | 1493916at2759 |
PhylomeDBi | O35532 |
TreeFami | TF354294 |
Enzyme and pathway databases
UniPathwayi | UPA00063 UPA00770;UER00756 |
Reactomei | R-RNO-191273, Cholesterol biosynthesis |
SABIO-RKi | O35532 |
Miscellaneous databases
PROi | PR:O35532 |
Gene expression databases
Bgeei | ENSRNOG00000032297, Expressed in female gonad and 22 other tissues |
Genevisiblei | O35532, RN |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSMO1_RAT | |
Accessioni | O35532Primary (citable) accession number: O35532 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 22, 2003 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families