UniProtKB - O35526 (STX1A_MOUSE)
Syntaxin-1A
Stx1a
Functioni
GO - Molecular functioni
- ATP-dependent protein binding Source: MGI
- calcium channel inhibitor activity Source: MGI
- calcium-dependent protein binding Source: MGI
- chloride channel inhibitor activity Source: MGI
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- kinase binding Source: MGI
- myosin binding Source: MGI
- myosin head/neck binding Source: MGI
- protein-containing complex binding Source: MGI
- protein domain specific binding Source: MGI
- protein-macromolecule adaptor activity Source: MGI
- protein N-terminus binding Source: MGI
- SNAP receptor activity Source: GO_Central
- SNARE binding Source: MGI
GO - Biological processi
- calcium-ion regulated exocytosis Source: MGI
- exocytosis Source: GO_Central
- hormone secretion Source: UniProtKB
- insulin secretion Source: UniProtKB
- intracellular protein transport Source: GO_Central
- modulation of excitatory postsynaptic potential Source: MGI
- positive regulation of calcium ion-dependent exocytosis Source: UniProtKB
- positive regulation of catecholamine secretion Source: UniProtKB
- positive regulation of excitatory postsynaptic potential Source: ParkinsonsUK-UCL
- positive regulation of exocytosis Source: MGI
- positive regulation of neurotransmitter secretion Source: ParkinsonsUK-UCL
- positive regulation of norepinephrine secretion Source: UniProtKB
- protein localization to membrane Source: MGI
- protein sumoylation Source: MGI
- regulated exocytosis Source: UniProtKB
- regulation of exocytosis Source: MGI
- regulation of synaptic vesicle priming Source: ParkinsonsUK-UCL
- response to gravity Source: MGI
- secretion by cell Source: MGI
- SNARE complex assembly Source: MGI
- synaptic vesicle docking Source: Ensembl
- synaptic vesicle endocytosis Source: MGI
- synaptic vesicle exocytosis Source: SynGO
- synaptic vesicle fusion to presynaptic active zone membrane Source: MGI
- vesicle docking Source: UniProtKB
- vesicle fusion Source: GO_Central
Keywordsi
Biological process | Exocytosis, Neurotransmitter transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-181429, Serotonin Neurotransmitter Release Cycle R-MMU-181430, Norepinephrine Neurotransmitter Release Cycle R-MMU-210500, Glutamate Neurotransmitter Release Cycle R-MMU-212676, Dopamine Neurotransmitter Release Cycle R-MMU-264642, Acetylcholine Neurotransmitter Release Cycle R-MMU-449836, Other interleukin signaling R-MMU-5682910, LGI-ADAM interactions R-MMU-888590, GABA synthesis, release, reuptake and degradation R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Syntaxin-1AAlternative name(s): Neuron-specific antigen HPC-1 |
Gene namesi | Name:Stx1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109355, Stx1a |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- synaptic vesicle membrane 1 Publication; Single-pass type IV membrane protein 1 Publication
- synaptosome 1 Publication
Note: Colocalizes with KCNB1 at the cell membrane.By similarity
Cytoskeleton
- actomyosin Source: MGI
Nucleus
- nuclear membrane Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: SynGO-UCL
- integral component of presynaptic membrane Source: MGI
- plasma membrane Source: MGI
- presynaptic active zone membrane Source: MGI
- presynaptic membrane Source: MGI
- voltage-gated potassium channel complex Source: Ensembl
Other locations
- acrosomal vesicle Source: MGI
- axon Source: SynGO-UCL
- endomembrane system Source: GO_Central
- glutamatergic synapse Source: MGI
- integral component of membrane Source: GO_Central
- integral component of synaptic vesicle membrane Source: MGI
- intracellular organelle Source: MGI
- membrane Source: MGI
- neuron projection Source: MGI
- postsynaptic density Source: MGI
- protein-containing complex Source: MGI
- secretory granule Source: MGI
- SNARE complex Source: UniProtKB
- synaptic vesicle Source: UniProtKB
- synaptic vesicle membrane Source: MGI
- synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 265 | CytoplasmicSequence analysisAdd BLAST | 265 | |
Transmembranei | 266 – 286 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 287 – 288 | ExtracellularSequence analysis | 2 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, SynaptosomePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210187 | 1 – 288 | Syntaxin-1AAdd BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineCombined sources | 1 | |
Modified residuei | 64 | PhosphoserineCombined sources | 1 | |
Modified residuei | 95 | PhosphoserineBy similarity | 1 | |
Modified residuei | 188 | Phosphoserine; by DAPK1By similarity | 1 | |
Cross-linki | 252 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 253 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 256 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | O35526 |
PeptideAtlasi | O35526 |
PRIDEi | O35526 |
PTM databases
iPTMneti | O35526 |
PhosphoSitePlusi | O35526 |
SwissPalmi | O35526 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000007207, Expressed in cerebral cortex and 254 other tissues |
Genevisiblei | O35526, MM |
Interactioni
Subunit structurei
Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (PubMed:28031464, PubMed:19196426). The SNARE complex interacts with CPLX1 (By similarity).
Interacts with STXBP1 (PubMed:28031464).
Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity).
Interacts with SYTL4 (PubMed:12101244).
Interacts with STXBP6 (By similarity).
Interacts with PLCL1 (via C2 domain) (PubMed:23341457).
Interacts with OTOF (PubMed:17055430).
Interacts with LGI3 (PubMed:18760330).
Interacts with SLC6A4 (By similarity).
Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent (PubMed:15774481).
Interacts with VAMP8 (PubMed:28031464).
Interacts with SNAP23 (PubMed:28031464).
Interacts with VAPA and SYBU (By similarity).
Interacts with PRRT2 (PubMed:29056747).
Interacts with SEPT8 (By similarity).
Interacts with STXBP5L (PubMed:21998599).
Interacts with synaptotagmin-1/SYT1 (By similarity).
By similarity9 PublicationsBinary interactionsi
Hide detailsO35526
GO - Molecular functioni
- ATP-dependent protein binding Source: MGI
- calcium-dependent protein binding Source: MGI
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- kinase binding Source: MGI
- myosin binding Source: MGI
- myosin head/neck binding Source: MGI
- protein domain specific binding Source: MGI
- protein N-terminus binding Source: MGI
- SNARE binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203561, 29 interactors |
CORUMi | O35526 |
DIPi | DIP-31950N |
IntActi | O35526, 22 interactors |
MINTi | O35526 |
STRINGi | 10090.ENSMUSP00000005509 |
Miscellaneous databases
RNActi | O35526, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 192 – 254 | t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 68 – 109 | Sequence analysisAdd BLAST | 42 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0810, Eukaryota |
GeneTreei | ENSGT01010000222508 |
HOGENOMi | CLU_042423_2_2_1 |
InParanoidi | O35526 |
OMAi | GQIRKQQ |
OrthoDBi | 1033833at2759 |
PhylomeDBi | O35526 |
TreeFami | TF313763 |
Family and domain databases
CDDi | cd00179, SynN, 1 hit |
InterProi | View protein in InterPro IPR010989, SNARE IPR028669, STX1 IPR006012, Syntaxin/epimorphin_CS IPR006011, Syntaxin_N IPR000727, T_SNARE_dom |
PANTHERi | PTHR19957:SF84, PTHR19957:SF84, 1 hit |
Pfami | View protein in Pfam PF05739, SNARE, 1 hit PF00804, Syntaxin, 1 hit |
SMARTi | View protein in SMART SM00503, SynN, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF47661, SSF47661, 1 hit |
PROSITEi | View protein in PROSITE PS00914, SYNTAXIN, 1 hit PS50192, T_SNARE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN
60 70 80 90 100
VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI
110 120 130 140 150
EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ
160 170 180 190 200
RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS
210 220 230 240 250
EIIKLETSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD
260 270 280
TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RFB9 | D6RFB9_MOUSE | Syntaxin-1A | Stx1a | 256 | Annotation score: | ||
A0A0J9YUA0 | A0A0J9YUA0_MOUSE | Syntaxin-1A | Stx1a | 158 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D45208 mRNA Translation: BAA28865.2 |
CCDSi | CCDS19731.1 |
RefSeqi | NP_058081.2, NM_016801.4 |
Genome annotation databases
Ensembli | ENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207 |
GeneIDi | 20907 |
KEGGi | mmu:20907 |
UCSCi | uc008zxk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D45208 mRNA Translation: BAA28865.2 |
CCDSi | CCDS19731.1 |
RefSeqi | NP_058081.2, NM_016801.4 |
3D structure databases
BMRBi | O35526 |
SMRi | O35526 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203561, 29 interactors |
CORUMi | O35526 |
DIPi | DIP-31950N |
IntActi | O35526, 22 interactors |
MINTi | O35526 |
STRINGi | 10090.ENSMUSP00000005509 |
PTM databases
iPTMneti | O35526 |
PhosphoSitePlusi | O35526 |
SwissPalmi | O35526 |
Proteomic databases
PaxDbi | O35526 |
PeptideAtlasi | O35526 |
PRIDEi | O35526 |
Protocols and materials databases
Antibodypediai | 3644, 671 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000005509; ENSMUSP00000005509; ENSMUSG00000007207 |
GeneIDi | 20907 |
KEGGi | mmu:20907 |
UCSCi | uc008zxk.1, mouse |
Organism-specific databases
CTDi | 6804 |
MGIi | MGI:109355, Stx1a |
Phylogenomic databases
eggNOGi | KOG0810, Eukaryota |
GeneTreei | ENSGT01010000222508 |
HOGENOMi | CLU_042423_2_2_1 |
InParanoidi | O35526 |
OMAi | GQIRKQQ |
OrthoDBi | 1033833at2759 |
PhylomeDBi | O35526 |
TreeFami | TF313763 |
Enzyme and pathway databases
Reactomei | R-MMU-181429, Serotonin Neurotransmitter Release Cycle R-MMU-181430, Norepinephrine Neurotransmitter Release Cycle R-MMU-210500, Glutamate Neurotransmitter Release Cycle R-MMU-212676, Dopamine Neurotransmitter Release Cycle R-MMU-264642, Acetylcholine Neurotransmitter Release Cycle R-MMU-449836, Other interleukin signaling R-MMU-5682910, LGI-ADAM interactions R-MMU-888590, GABA synthesis, release, reuptake and degradation R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Miscellaneous databases
BioGRID-ORCSi | 20907, 0 hits in 17 CRISPR screens |
ChiTaRSi | Stx1a, mouse |
PROi | PR:O35526 |
RNActi | O35526, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007207, Expressed in cerebral cortex and 254 other tissues |
Genevisiblei | O35526, MM |
Family and domain databases
CDDi | cd00179, SynN, 1 hit |
InterProi | View protein in InterPro IPR010989, SNARE IPR028669, STX1 IPR006012, Syntaxin/epimorphin_CS IPR006011, Syntaxin_N IPR000727, T_SNARE_dom |
PANTHERi | PTHR19957:SF84, PTHR19957:SF84, 1 hit |
Pfami | View protein in Pfam PF05739, SNARE, 1 hit PF00804, Syntaxin, 1 hit |
SMARTi | View protein in SMART SM00503, SynN, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF47661, SSF47661, 1 hit |
PROSITEi | View protein in PROSITE PS00914, SYNTAXIN, 1 hit PS50192, T_SNARE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STX1A_MOUSE | |
Accessioni | O35526Primary (citable) accession number: O35526 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | December 19, 2001 | |
Last modified: | February 10, 2021 | |
This is version 186 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families