UniProtKB - O35495 (CDK14_MOUSE)
Cyclin-dependent kinase 14
Cdk14
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 164 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 256 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 141 – 149 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin binding Source: MGI
- cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- protein kinase activity Source: MGI
- protein serine/threonine kinase activity Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- G2/M transition of mitotic cell cycle Source: UniProtKB
- protein phosphorylation Source: GO_Central
- regulation of canonical Wnt signaling pathway Source: UniProtKB
- regulation of transcription involved in G1/S transition of mitotic cell cycle Source: GO_Central
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Wnt signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-dependent kinase 14 (EC:2.7.11.22)Alternative name(s): Cell division protein kinase 14 Serine/threonine-protein kinase PFTAIRE-1 |
Gene namesi | Name:Cdk14 Synonyms:Kiaa0834, Pftk1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894318, Cdk14 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Nucleus
Other locations
Note: Recruited to the cell membrane by CCNY.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: MGI
- cytoplasmic cyclin-dependent protein kinase holoenzyme complex Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086507 | 1 – 469 | Cyclin-dependent kinase 14Add BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | PhosphoserineBy similarity | 1 | |
Modified residuei | 78 | PhosphoserineBy similarity | 1 | |
Modified residuei | 95 | PhosphoserineCombined sources | 1 | |
Modified residuei | 134 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O35495 |
MaxQBi | O35495 |
PaxDbi | O35495 |
PeptideAtlasi | O35495 |
PRIDEi | O35495 |
PTM databases
iPTMneti | O35495 |
PhosphoSitePlusi | O35495 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000028926, Expressed in dentate gyrus of hippocampal formation and 307 other tissues |
Genevisiblei | O35495, MM |
Interactioni
Subunit structurei
Found in a complex with LRP6, CCNY and CAPRIN2 during G2/M stage; CAPRIN2 functions as a scaffold for the complex by binding to CCNY via its N terminus and to CDK14 via its C terminus.
Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6.
Interacts with CCDN3 and CDKN1A.
Interacts with SEPT8.
Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ.
By similarityGO - Molecular functioni
- cyclin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202129, 8 interactors |
ComplexPortali | CPX-367, Cyclin Y-Cdk14 complex |
IntActi | O35495, 1 interactor |
MINTi | O35495 |
STRINGi | 10090.ENSMUSP00000030763 |
Miscellaneous databases
RNActi | O35495, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 135 – 419 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 285 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000157640 |
HOGENOMi | CLU_000288_181_6_1 |
InParanoidi | O35495 |
OMAi | NSRHGCA |
OrthoDBi | 1010560at2759 |
TreeFami | TF106508 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR
60 70 80 90 100
NCQGTDSVIK HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC
110 120 130 140 150
INFKSSSAGK ESPKVRRHSS PSSPTSPKFG KADSYEKLEK LGEGSYATVY
160 170 180 190 200
KGKSKVNGKL VALKVIRLQE EEGTPFTAIR EASLLKGLKH ANIVLLHDII
210 220 230 240 250
HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL LRGLSYIHQR
260 270 280 290 300
YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP
310 320 330 340 350
DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG
360 370 380 390 400
TPNEDTWPGV HSLPHFKPER FTVYSSKSLR QAWNKLSYVN HAEDLASKLL
410 420 430 440 450
QCSPKNRLSA QAALSHEYFS DLPPRLWELT DMSSIFTVPN VRLQPEAGES
460
MRAFGKNNSY GKSLSNSKH
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PVC7 | E9PVC7_MOUSE | Cyclin-dependent kinase 14 | Cdk14 | 451 | Annotation score: | ||
E9Q9M2 | E9Q9M2_MOUSE | Cyclin-dependent kinase 14 | Cdk14 | 167 | Annotation score: | ||
D3YV23 | D3YV23_MOUSE | Cyclin-dependent kinase 14 | Cdk14 | 92 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 219 | Q → R in BAE38049 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 222 – 223 | DK → EQ in AAB87504 (PubMed:9547506).Curated | 2 | |
Sequence conflicti | 375 | S → N in AAB70455 (PubMed:9202329).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038763 | 1 – 46 | Missing in isoform 2. 2 PublicationsAdd BLAST | 46 | |
Alternative sequenceiVSP_038764 | 349 – 469 | LGTPN…SNSKH → SSAQSASQKNLFLISYLVT in isoform 3. 1 PublicationAdd BLAST | 121 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033655 mRNA Translation: AAB87504.1 U62391 mRNA Translation: AAB70455.1 AK165149 mRNA Translation: BAE38049.1 AK051283 mRNA Translation: BAE43346.1 AK129226 mRNA Translation: BAC98036.1 |
CCDSi | CCDS19073.1 [O35495-1] CCDS80203.1 [O35495-2] |
RefSeqi | NP_035204.2, NM_011074.3 [O35495-1] |
Genome annotation databases
Ensembli | ENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926 [O35495-1] ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926 [O35495-2] ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926 [O35495-2] |
GeneIDi | 18647 |
KEGGi | mmu:18647 |
UCSCi | uc008wih.2, mouse [O35495-1] uc008wik.2, mouse [O35495-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033655 mRNA Translation: AAB87504.1 U62391 mRNA Translation: AAB70455.1 AK165149 mRNA Translation: BAE38049.1 AK051283 mRNA Translation: BAE43346.1 AK129226 mRNA Translation: BAC98036.1 |
CCDSi | CCDS19073.1 [O35495-1] CCDS80203.1 [O35495-2] |
RefSeqi | NP_035204.2, NM_011074.3 [O35495-1] |
3D structure databases
SMRi | O35495 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202129, 8 interactors |
ComplexPortali | CPX-367, Cyclin Y-Cdk14 complex |
IntActi | O35495, 1 interactor |
MINTi | O35495 |
STRINGi | 10090.ENSMUSP00000030763 |
PTM databases
iPTMneti | O35495 |
PhosphoSitePlusi | O35495 |
Proteomic databases
jPOSTi | O35495 |
MaxQBi | O35495 |
PaxDbi | O35495 |
PeptideAtlasi | O35495 |
PRIDEi | O35495 |
Protocols and materials databases
Antibodypediai | 15462, 226 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926 [O35495-1] ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926 [O35495-2] ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926 [O35495-2] |
GeneIDi | 18647 |
KEGGi | mmu:18647 |
UCSCi | uc008wih.2, mouse [O35495-1] uc008wik.2, mouse [O35495-3] |
Organism-specific databases
CTDi | 5218 |
MGIi | MGI:894318, Cdk14 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000157640 |
HOGENOMi | CLU_000288_181_6_1 |
InParanoidi | O35495 |
OMAi | NSRHGCA |
OrthoDBi | 1010560at2759 |
TreeFami | TF106508 |
Miscellaneous databases
BioGRID-ORCSi | 18647, 0 hits in 18 CRISPR screens |
ChiTaRSi | Cdk14, mouse |
PROi | PR:O35495 |
RNActi | O35495, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028926, Expressed in dentate gyrus of hippocampal formation and 307 other tissues |
Genevisiblei | O35495, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK14_MOUSE | |
Accessioni | O35495Primary (citable) accession number: O35495 Secondary accession number(s): O35848 Q6ZQ37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2002 |
Last sequence update: | March 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families