Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - O35495 (CDK14_MOUSE)

Protein

Cyclin-dependent kinase 14

Gene

Cdk14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Serine/threonine-protein kinase activity is promoted by associated cyclins CCDN3 and CCNY and repressed by CDKN1A.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164ATPPROSITE-ProRule annotation1
Active sitei256Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 149ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin binding Source: MGI
  • cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  • protein kinase activity Source: MGI
  • protein serine/threonine kinase activity Source: MGI
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 14 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 14
Serine/threonine-protein kinase PFTAIRE-1
Gene namesi
Name:Cdk14
Synonyms:Kiaa0834, Pftk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:894318 Cdk14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865071 – 469Cyclin-dependent kinase 14Add BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei95PhosphoserineCombined sources1
Modified residuei134PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO35495
PaxDbiO35495
PeptideAtlasiO35495
PRIDEiO35495

PTM databases

iPTMnetiO35495
PhosphoSitePlusiO35495

Expressioni

Tissue specificityi

In the adult, widely expressed at low levels except in brain, kidney and testis where expression is high. In the brain, detected in cortex, hippocampus, dentate gyrus, amygdala cortex, parasubiculum and cerebellum. In the embryo, expressed predominantly in the nervous system.1 Publication

Developmental stagei

In the testis, expressed at low levels in Sertoli cells of 7-day-old mice, barely detected at day 17, and detected at much higher levels in late pachytene/diplotene spermatocytes in the adult. In the nervous system, expressed at highest levels in the adult.2 Publications

Gene expression databases

BgeeiENSMUSG00000028926 Expressed in 292 organ(s), highest expression level in dentate gyrus of hippocampal formation
CleanExiMM_PFTK1
ExpressionAtlasiO35495 baseline and differential
GenevisibleiO35495 MM

Interactioni

Subunit structurei

Found in a complex with LRP6, CCNY and CAPRIN2 during G2/M stage; CAPRIN2 functions as a scaffold for the complex by binding to CCNY via its N terminus and to CDK14 via its C terminus. Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6. Interacts with CCDN3 and CDKN1A. Interacts with SEPT8. Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ.By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi202129, 5 interactors
ComplexPortaliCPX-367 Cyclin Y-Cdk14 complex
IntActiO35495, 1 interactor
MINTiO35495
STRINGi10090.ENSMUSP00000030763

Structurei

3D structure databases

ProteinModelPortaliO35495
SMRiO35495
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 419Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Curated

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
GeneTreeiENSGT00900000140869
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiO35495
KOiK08821
OMAiPERFTQY
OrthoDBiEOG091G0CH0
TreeFamiTF106508

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O35495-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKEDTTFD EICVTKMSTR 
        60         70         80         90        100
NCQGTDSVIK HLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC 
       110        120        130        140        150
INFKSSSAGK ESPKVRRHSS PSSPTSPKFG KADSYEKLEK LGEGSYATVY 
       160        170        180        190        200
KGKSKVNGKL VALKVIRLQE EEGTPFTAIR EASLLKGLKH ANIVLLHDII 
       210        220        230        240        250
HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL LRGLSYIHQR 
       260        270        280        290        300
YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP 
       310        320        330        340        350
DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG 
       360        370        380        390        400
TPNEDTWPGV HSLPHFKPER FTVYSSKSLR QAWNKLSYVN HAEDLASKLL 
       410        420        430        440        450
QCSPKNRLSA QAALSHEYFS DLPPRLWELT DMSSIFTVPN VRLQPEAGES 
       460 
MRAFGKNNSY GKSLSNSKH
Length:469
Mass (Da):52,996
Last modified:March 2, 2010 - v2
Checksum:i2042D0A66655A8B0
GO
Isoform 2 (identifier: O35495-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Show »
Length:423
Mass (Da):47,684
Checksum:i5895A066A7C17AAB
GO
Isoform 3 (identifier: O35495-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-469: LGTPNEDTWP...YGKSLSNSKH → SSAQSASQKNLFLISYLVT

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):41,378
Checksum:i16A34E81C94C526B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PVC7E9PVC7_MOUSE
Cyclin-dependent kinase 14
Cdk14
451Annotation score:
E9Q9M2E9Q9M2_MOUSE
Cyclin-dependent kinase 14
Cdk14
167Annotation score:
D3YV23D3YV23_MOUSE
Cyclin-dependent kinase 14
Cdk14
92Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219Q → R in BAE38049 (PubMed:16141072).Curated1
Sequence conflicti222 – 223DK → EQ in AAB87504 (PubMed:9547506).Curated2
Sequence conflicti375S → N in AAB70455 (PubMed:9202329).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0387631 – 46Missing in isoform 2. 2 PublicationsAdd BLAST46
Alternative sequenceiVSP_038764349 – 469LGTPN…SNSKH → SSAQSASQKNLFLISYLVT in isoform 3. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033655 mRNA Translation: AAB87504.1
U62391 mRNA Translation: AAB70455.1
AK165149 mRNA Translation: BAE38049.1
AK051283 mRNA Translation: BAE43346.1
AK129226 mRNA Translation: BAC98036.1
CCDSiCCDS19073.1 [O35495-1]
CCDS80203.1 [O35495-2]
RefSeqiNP_035204.2, NM_011074.3 [O35495-1]
UniGeneiMm.389061
Mm.404502

Genome annotation databases

EnsembliENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926 [O35495-1]
ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926 [O35495-2]
ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926 [O35495-2]
GeneIDi18647
KEGGimmu:18647
UCSCiuc008wih.2 mouse [O35495-1]
uc008wik.2 mouse [O35495-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033655 mRNA Translation: AAB87504.1
U62391 mRNA Translation: AAB70455.1
AK165149 mRNA Translation: BAE38049.1
AK051283 mRNA Translation: BAE43346.1
AK129226 mRNA Translation: BAC98036.1
CCDSiCCDS19073.1 [O35495-1]
CCDS80203.1 [O35495-2]
RefSeqiNP_035204.2, NM_011074.3 [O35495-1]
UniGeneiMm.389061
Mm.404502

3D structure databases

ProteinModelPortaliO35495
SMRiO35495
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202129, 5 interactors
ComplexPortaliCPX-367 Cyclin Y-Cdk14 complex
IntActiO35495, 1 interactor
MINTiO35495
STRINGi10090.ENSMUSP00000030763

PTM databases

iPTMnetiO35495
PhosphoSitePlusiO35495

Proteomic databases

MaxQBiO35495
PaxDbiO35495
PeptideAtlasiO35495
PRIDEiO35495

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030763; ENSMUSP00000030763; ENSMUSG00000028926 [O35495-1]
ENSMUST00000115450; ENSMUSP00000111110; ENSMUSG00000028926 [O35495-2]
ENSMUST00000115451; ENSMUSP00000111111; ENSMUSG00000028926 [O35495-2]
GeneIDi18647
KEGGimmu:18647
UCSCiuc008wih.2 mouse [O35495-1]
uc008wik.2 mouse [O35495-3]

Organism-specific databases

CTDi5218
MGIiMGI:894318 Cdk14
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
GeneTreeiENSGT00900000140869
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiO35495
KOiK08821
OMAiPERFTQY
OrthoDBiEOG091G0CH0
TreeFamiTF106508

Miscellaneous databases

ChiTaRSiCdk14 mouse
PROiPR:O35495
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028926 Expressed in 292 organ(s), highest expression level in dentate gyrus of hippocampal formation
CleanExiMM_PFTK1
ExpressionAtlasiO35495 baseline and differential
GenevisibleiO35495 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDK14_MOUSE
AccessioniPrimary (citable) accession number: O35495
Secondary accession number(s): O35848
, Q3TNN9, Q3V319, Q6ZQ37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 2, 2010
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again