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Protein

Dual specificity protein kinase CLK3

Gene

Clk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Activity regulationi

Leucettine L41 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei431Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi310 – 318ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK3 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 3
Gene namesi
Name:Clk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1098670 Clk3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075282
GuidetoPHARMACOLOGYi1992

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858711 – 638Dual specificity protein kinase CLK3Add BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphotyrosineBy similarity1
Modified residuei157PhosphoserineCombined sources1
Modified residuei197PhosphoserineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei224PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei283PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylates on all three types of residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35492
MaxQBiO35492
PaxDbiO35492
PeptideAtlasiO35492
PRIDEiO35492

PTM databases

iPTMnetiO35492
PhosphoSitePlusiO35492

Expressioni

Tissue specificityi

Present at high levels in testis and ovary. In testis, expression is restricted to elongated, maturing spermatozoa. Also present in spleen, brain, lung and liver (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000032316 Expressed in 297 organ(s), highest expression level in thymus
CleanExiMM_CLK3
ExpressionAtlasiO35492 baseline and differential
GenevisibleiO35492 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221868, 2 interactors
IntActiO35492, 1 interactor
MINTiO35492
STRINGi10090.ENSMUSP00000067341

Chemistry databases

BindingDBiO35492

Structurei

3D structure databases

ProteinModelPortaliO35492
SMRiO35492
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini304 – 620Protein kinasePROSITE-ProRule annotationAdd BLAST317

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 264Arg-richAdd BLAST258

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0671 Eukaryota
ENOG410XQF2 LUCA
GeneTreeiENSGT00580000081366
HOGENOMiHOG000203417
HOVERGENiHBG107720
InParanoidiO35492
KOiK08823
OrthoDBiEOG091G033A
PhylomeDBiO35492
TreeFamiTF101041

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O35492-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPVLSARRKR LASTAGPRRG SGPSLAVRWV PPLGPEPSSD RGRAPMRPRG
60 70 80 90 100
PTCSTTRRGA GRGPRLLPGP PGRDLHRCRP DPGGAGQSPR VCEFGARAVR
110 120 130 140 150
PLGRVEPGPP TAASREGAVL PRAEARAGSG RGARSGEWGL AAAGAWETMH
160 170 180 190 200
HCKRYRSPEP DPYLSYRWKR RRSYSREHEG RLRYPSRREP PPRRSRSRSH
210 220 230 240 250
DRIPYQRRYR EHRDSDTYRC EERSPSFGED CYGSSRSRHR RRSRERAPYR
260 270 280 290 300
TRKHAHHCHK RRTRSCSSAS SRSQQSSKRS SRSVEDDKEG HLVCRIGDWL
310 320 330 340 350
QERYEIVGNL GEGTFGKVVE CLDHARGKSQ VALKIIRNVG KYREAARLEI
360 370 380 390 400
NVLKKIKEKD KENKFLCVLM SDWFNFHGHM CIAFELLGKN TFEFLKENNF
410 420 430 440 450
QPYPLPHVRH MAYQLCHALR FLHENQLTHT DLKPENILFV NSEFETLYNE
460 470 480 490 500
HKSCEEKSVK NTSIRVADFG SATFDHEHHT TIVATRHYRP PEVILELGWA
510 520 530 540 550
QPCDVWSIGC ILFEYYRGFT LFQTHENREH LVMMEKILGP IPSHMIHRTR
560 570 580 590 600
KQKYFYKGGL VWDENSSDGR YVKENCKPLK SYMLQDSLEH VQLFDLMRRM
610 620 630
LEFDPAQRIT LAEALLHPFF AGLTPEERSF HSSRNPSR
Length:638
Mass (Da):73,798
Last modified:June 12, 2007 - v2
Checksum:i3CE7BFD35FB09A3C
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SST3A0A1L1SST3_MOUSE
Dual-specificity protein kinase CLK...
Clk3
478Annotation score:
A0A1L1SQZ6A0A1L1SQZ6_MOUSE
Dual-specificity protein kinase CLK...
Clk3
263Annotation score:
A0A1L1SQT5A0A1L1SQT5_MOUSE
Dual-specificity protein kinase CLK...
Clk3
191Annotation score:
A0A1L1STP6A0A1L1STP6_MOUSE
Dual-specificity protein kinase CLK...
Clk3
90Annotation score:

Sequence cautioni

The sequence AAB87509 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH85084 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC41138 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE21937 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE32308 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE39405 differs from that shown. Reason: Frameshift at position 15.Curated
The sequence BAE39405 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68P → T in BAE39405 (PubMed:16141072).Curated1
Sequence conflicti165S → T in AAB87509 (PubMed:9307018).Curated1
Sequence conflicti314T → L in BAE43216 (PubMed:16141072).Curated1
Sequence conflicti428T → A in BAC41138 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090215 mRNA Translation: BAC41138.1 Different initiation.
AK133936 mRNA Translation: BAE21937.1 Different initiation.
AK146942 mRNA Translation: BAE27551.1
AK154003 mRNA Translation: BAE32308.1 Different initiation.
AK167300 mRNA Translation: BAE39405.1 Sequence problems.
AK009908 mRNA Translation: BAE43216.1
BC085084 mRNA Translation: AAH85084.1 Different initiation.
AF033565 mRNA Translation: AAB87509.1 Different initiation.
CCDSiCCDS23232.1
RefSeqiNP_031739.3, NM_007713.4
XP_006510802.1, XM_006510739.3
XP_017168549.1, XM_017313060.1
UniGeneiMm.25720

Genome annotation databases

EnsembliENSMUST00000065330; ENSMUSP00000067341; ENSMUSG00000032316
GeneIDi102414
KEGGimmu:102414
UCSCiuc009pvr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090215 mRNA Translation: BAC41138.1 Different initiation.
AK133936 mRNA Translation: BAE21937.1 Different initiation.
AK146942 mRNA Translation: BAE27551.1
AK154003 mRNA Translation: BAE32308.1 Different initiation.
AK167300 mRNA Translation: BAE39405.1 Sequence problems.
AK009908 mRNA Translation: BAE43216.1
BC085084 mRNA Translation: AAH85084.1 Different initiation.
AF033565 mRNA Translation: AAB87509.1 Different initiation.
CCDSiCCDS23232.1
RefSeqiNP_031739.3, NM_007713.4
XP_006510802.1, XM_006510739.3
XP_017168549.1, XM_017313060.1
UniGeneiMm.25720

3D structure databases

ProteinModelPortaliO35492
SMRiO35492
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221868, 2 interactors
IntActiO35492, 1 interactor
MINTiO35492
STRINGi10090.ENSMUSP00000067341

Chemistry databases

BindingDBiO35492
ChEMBLiCHEMBL1075282
GuidetoPHARMACOLOGYi1992

PTM databases

iPTMnetiO35492
PhosphoSitePlusiO35492

Proteomic databases

EPDiO35492
MaxQBiO35492
PaxDbiO35492
PeptideAtlasiO35492
PRIDEiO35492

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065330; ENSMUSP00000067341; ENSMUSG00000032316
GeneIDi102414
KEGGimmu:102414
UCSCiuc009pvr.1 mouse

Organism-specific databases

CTDi1198
MGIiMGI:1098670 Clk3

Phylogenomic databases

eggNOGiKOG0671 Eukaryota
ENOG410XQF2 LUCA
GeneTreeiENSGT00580000081366
HOGENOMiHOG000203417
HOVERGENiHBG107720
InParanoidiO35492
KOiK08823
OrthoDBiEOG091G033A
PhylomeDBiO35492
TreeFamiTF101041

Enzyme and pathway databases

BRENDAi2.7.12.1 3474

Miscellaneous databases

ChiTaRSiClk3 mouse
PROiPR:O35492
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032316 Expressed in 297 organ(s), highest expression level in thymus
CleanExiMM_CLK3
ExpressionAtlasiO35492 baseline and differential
GenevisibleiO35492 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLK3_MOUSE
AccessioniPrimary (citable) accession number: O35492
Secondary accession number(s): Q3TJU0
, Q3UIF5, Q3V463, Q5U4I1, Q8C1V1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 12, 2007
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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