Slc27a2

Functionⁱ

Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precursors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism (By similarity). May be involved in translocation of long-chain fatty acids (LFCA) across membranes.By similarity1 Publication

Catalytic activityⁱ

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

ATP + a very-long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	222 – 233	AMPSequence analysisAdd BLAST		12

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
decanoate-CoA ligase activity Source: UniProtKB-EC
enzyme binding Source: MGI
fatty acid transmembrane transporter activity
Source: MGI
Inferred from direct assayⁱ
- 9671728
long-chain fatty acid-CoA ligase activity
Source: MGI
Inferred from direct assayⁱ
- 12048192
long-chain fatty acid transporter activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
phytanate-CoA ligase activity Source: MGI
pristanate-CoA ligase activity Source: MGI
signaling receptor binding Source: MGI
very long-chain fatty acid-CoA ligase activity
Source: MGI
Inferred from direct assayⁱ
- 12048192

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bile acid biosynthetic process Source: MGI
fatty acid alpha-oxidation Source: MGI
fatty acid beta-oxidation Source: MGI
fatty acid transport
Source: MGI
Inferred from direct assayⁱ
- 9271215
long-chain fatty acid import Source: MGI
long-chain fatty acid metabolic process
Source: MGI
Inferred from direct assayⁱ
- 12048192
methyl-branched fatty acid metabolic process Source: MGI
very long-chain fatty acid catabolic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 12719378
very long-chain fatty acid metabolic process
Source: MGI
Inferred from direct assayⁱ
- 12048192

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Fatty acid metabolism, Lipid metabolism
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol R-MMU-389599 Alpha-oxidation of phytanate R-MMU-6798695 Neutrophil degranulation R-MMU-9033241 Peroxisomal protein import

Reactomeⁱ

R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-389599 Alpha-oxidation of phytanate
R-MMU-6798695 Neutrophil degranulation
R-MMU-9033241 Peroxisomal protein import

Chemistry databases

SwissLipidsⁱ	SLP:000000430

SwissLipidsⁱ

SLP:000000430

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Very long-chain acyl-CoA synthetase (EC:6.2.1.-) Short name: VLACS Short name: VLCS Alternative name(s): Fatty acid transport protein 2 Short name: FATP-2 Fatty-acid-coenzyme A ligase, very long-chain 1 Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3) Solute carrier family 27 member 2 THCA-CoA ligase Very long-chain-fatty-acid-CoA ligase
Gene namesⁱ	Name:Slc27a2 Synonyms:Acsvl1, Facvl1, Fatp2, Vlacs, Vlcs
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:1347099 Slc27a2

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 4	LumenalBy similarity	4
Transmembraneⁱ	5 – 27	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	28 – 106	CytoplasmicSequence analysisAdd BLAST	79
Transmembraneⁱ	107 – 127	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	128 – 267	LumenalSequence analysisAdd BLAST	140
Transmembraneⁱ	268 – 288	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	289 – 620	CytoplasmicBy similarityAdd BLAST	332

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane, Peroxisome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000193205	1 – 620	Very long-chain acyl-CoA synthetaseAdd BLAST		620

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	291	N6-acetyllysineCombined sources		1
Modified residueⁱ	577	PhosphothreonineCombined sources		1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O35488
PaxDbⁱ	O35488
PeptideAtlas More...PeptideAtlasⁱ	O35488
PRIDEⁱ	O35488

PTM databases

iPTMnetⁱ	O35488
PhosphoSitePlusⁱ	O35488
SwissPalmⁱ	O35488

Expressionⁱ

Tissue specificityⁱ

Strong expression in liver and kidney, low expression in brain and testis, no expression in skeletal muscle and spleen. Shows uniform distribution in liver acinus.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000027359 Expressed in 221 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasⁱ	O35488 baseline and differential
Genevisibleⁱ	O35488 MM

Interactionⁱ

GO - Molecular functionⁱ

enzyme binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	O35488, 4 interactors
Molecular INTeraction database More...MINTⁱ	O35488
STRINGⁱ	10090.ENSMUSP00000057595

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O35488
SMRⁱ	O35488
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ATP-dependent AMP-binding enzyme family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1179 Eukaryota ENOG410XQ8T LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074420
HOGENOMⁱ	HOG000044189
HOVERGENⁱ	HBG005642
InParanoidⁱ	O35488
KEGG Orthology (KO) More...KOⁱ	K08746
OMAⁱ	PMACLNY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0B76
TreeFamⁱ	TF313430

Family and domain databases

InterProⁱ	View protein in InterPro IPR025110 AMP-bd_C IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR030305 FATP2
PANTHERⁱ	PTHR43107:SF13 PTHR43107:SF13, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00501 AMP-binding, 1 hit PF13193 AMP-binding_C, 1 hit
PROSITEⁱ	View protein in PROSITE PS00455 AMP_BINDING, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O35488-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLPVLYTGLA GLLLLPLLLT CCCPYLLQDV RYFLRLANMA RRVRSYRQRR 
        60         70         80         90        100
PVRTILRAFL EQARKTPHKP FLLFRDETLT YAQVDRRSNQ VARALHDQLG 
       110        120        130        140        150
LRQGDCVALF MGNEPAYVWI WLGLLKLGCP MACLNYNIRA KSLLHCFQCC 
       160        170        180        190        200
GAKVLLASPD LQEAVEEVLP TLKKDAVSVF YVSRTSNTNG VDTILDKVDG 
       210        220        230        240        250
VSAEPTPESW RSEVTFTTPA VYIYTSGTTG LPKAATINHH RLWYGTGLAM 
       260        270        280        290        300
SSGITAQDVI YTTMPLYHSA ALMIGLHGCI VVGATLALRS KFSASQFWDD 
       310        320        330        340        350
CRKYNVTVIQ YIGELLRYLC NTPQKPNDRD HKVKKALGNG LRGDVWREFI 
       360        370        380        390        400
KRFGDIHVYE FYASTEGNIG FVNYPRKIGA VGRANYLQRK VARYELIKYD 
       410        420        430        440        450
VEKDEPVRDA NGYCIKVPKG EVGLLVCKIT QLTPFIGYAG GKTQTEKKKL 
       460        470        480        490        500
RDVFKKGDIY FNSGDLLMID RENFVYFHDR VGDTFRWKGE NVATTEVADI 
       510        520        530        540        550
VGLVDFVEEV NVYGVPVPGH EGRIGMASLK IKENYEFNGK KLFQHIAEYL 
       560        570        580        590        600
PSYARPRFLR IQDTIEITGT FKHRKVTLME EGFNPTVIKD TLYFMDDAEK 
       610        620
TFVPMTENIY NAIIDKTLKL

Length:620

Mass (Da):70,423

Last modified:July 27, 2011 - v2

Checksum:ⁱ62994BDB1D828B37

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A2ANX6	A2ANX6_MOUSE		Very long-chain acyl-CoA synthetase Very long-chain acyl-CoA synthetase	Slc27a2	484	Annotation score:

Sequence cautionⁱ

The sequence AAC40186 differs from that shown. Reason: Frameshift at positions 253, 263, 267, 281, 289, 293, 299, 301 and 307.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	35	R → Q in AAB87982 (PubMed:11980911).Curated	1
Sequence conflictⁱ	168	V → A in CAA11687 (PubMed:9559670).Curated	1
Sequence conflictⁱ	168	V → A in AAB87982 (PubMed:11980911).Curated	1
Sequence conflictⁱ	234 – 235	AA → SG in AAC40186 (PubMed:9671728).Curated	2
Sequence conflictⁱ	243	W → R in AAC40186 (PubMed:9671728).Curated	1
Sequence conflictⁱ	247	G → S in AAC40186 (PubMed:9671728).Curated	1
Sequence conflictⁱ	257	Q → K in AAC40186 (PubMed:9671728).Curated	1
Sequence conflictⁱ	271	A → T in AAC40186 (PubMed:9671728).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ223958 mRNA Translation: CAA11687.1 AF072757 mRNA Translation: AAC40186.1 Sequence problems. AF033031 mRNA Translation: AAB87982.1 AL831764, AL844555 Genomic DNA Translation: CAM17405.1 AL844555, AL831764 Genomic DNA Translation: CAM18704.1 BC013442 mRNA Translation: AAH13442.1 BC022170 mRNA Translation: AAH22170.1 BC024735 mRNA Translation: AAH24735.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16683.1
NCBI Reference Sequences More...RefSeqⁱ	NP_036108.2, NM_011978.2
UniGeneⁱ	Mm.290044

Genome annotation databases

Ensemblⁱ	ENSMUST00000061491; ENSMUSP00000057595; ENSMUSG00000027359
GeneIDⁱ	26458
KEGGⁱ	mmu:26458
UCSC genome browser More...UCSCⁱ	uc008mdq.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O35488	Uncharacterized protein (Fragment)		MOUSE		443	UniRef100_O35488

Protein	Similar proteins		Species	Score	Length	Source
O35488	Very long-chain acyl-CoA synthetase		RAT		620	UniRef90_O35488
Uncharacterized protein (Fragment)		MOUSE		443
very long-chain acyl-CoA synthetase		MESAU		620
UPI00042ABAAD		PERMB		620
UPI000A313BA4		MUSPA		620
+8

Protein	Similar proteins		Species	Score	Length	Source
O35488	Very long-chain acyl-CoA synthetase		HUMAN		620	UniRef50_O14975
Very long-chain acyl-CoA synthetase		RAT		620
Solute carrier family 27 member 2		SAIBB		754
Solute carrier family 27 member 2		PONAB		620
Uncharacterized protein		PANPA		620
+714

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ223958 mRNA Translation: CAA11687.1 AF072757 mRNA Translation: AAC40186.1 Sequence problems. AF033031 mRNA Translation: AAB87982.1 AL831764, AL844555 Genomic DNA Translation: CAM17405.1 AL844555, AL831764 Genomic DNA Translation: CAM18704.1 BC013442 mRNA Translation: AAH13442.1 BC022170 mRNA Translation: AAH22170.1 BC024735 mRNA Translation: AAH24735.1
CCDSⁱ	CCDS16683.1
RefSeqⁱ	NP_036108.2, NM_011978.2
UniGeneⁱ	Mm.290044

3D structure databases

ProteinModelPortalⁱ	O35488
SMRⁱ	O35488
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	O35488, 4 interactors
MINTⁱ	O35488
STRINGⁱ	10090.ENSMUSP00000057595

Chemistry databases

SwissLipidsⁱ	SLP:000000430

SwissLipidsⁱ

SLP:000000430

PTM databases

iPTMnetⁱ	O35488
PhosphoSitePlusⁱ	O35488
SwissPalmⁱ	O35488

Proteomic databases

MaxQBⁱ	O35488
PaxDbⁱ	O35488
PeptideAtlasⁱ	O35488
PRIDEⁱ	O35488

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000061491; ENSMUSP00000057595; ENSMUSG00000027359
GeneIDⁱ	26458
KEGGⁱ	mmu:26458
UCSCⁱ	uc008mdq.1 mouse

Organism-specific databases

CTDⁱ	11001
MGIⁱ	MGI:1347099 Slc27a2

Phylogenomic databases

eggNOGⁱ	KOG1179 Eukaryota ENOG410XQ8T LUCA
GeneTreeⁱ	ENSGT00550000074420
HOGENOMⁱ	HOG000044189
HOVERGENⁱ	HBG005642
InParanoidⁱ	O35488
KOⁱ	K08746
OMAⁱ	PMACLNY
OrthoDBⁱ	EOG091G0B76
TreeFamⁱ	TF313430

Enzyme and pathway databases

Reactomeⁱ	R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol R-MMU-389599 Alpha-oxidation of phytanate R-MMU-6798695 Neutrophil degranulation R-MMU-9033241 Peroxisomal protein import

Reactomeⁱ

R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-389599 Alpha-oxidation of phytanate
R-MMU-6798695 Neutrophil degranulation
R-MMU-9033241 Peroxisomal protein import

Miscellaneous databases

ChiTaRSⁱ	Slc27a2 mouse
Protein Ontology More...PROⁱ	PR:O35488
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027359 Expressed in 221 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasⁱ	O35488 baseline and differential
Genevisibleⁱ	O35488 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR025110 AMP-bd_C IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR030305 FATP2
PANTHERⁱ	PTHR43107:SF13 PTHR43107:SF13, 1 hit
Pfamⁱ	View protein in Pfam PF00501 AMP-binding, 1 hit PF13193 AMP-binding_C, 1 hit
PROSITEⁱ	View protein in PROSITE PS00455 AMP_BINDING, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	S27A2_MOUSE
Accessionⁱ	O35488Primary (citable) accession number: O35488 Secondary accession number(s): O70550, O88560, Q91WV6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O35488 (S27A2_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Very long-chain acyl-CoA synthetase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Peroxisome

Endoplasmic reticulum

Endoplasmic reticulum

Mitochondrion

Peroxisome

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ