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Protein

EGF-like repeat and discoidin I-like domain-containing protein 3

Gene

Edil3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119CalciumBy similarity1
Metal bindingi120Calcium; via carbonyl oxygenBy similarity1
Metal bindingi122CalciumBy similarity1
Metal bindingi136CalciumBy similarity1
Metal bindingi137Calcium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processCell adhesion
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EGF-like repeat and discoidin I-like domain-containing protein 3
Alternative name(s):
Developmentally-regulated endothelial cell locus 1 protein
Integrin-binding protein DEL1
Gene namesi
Name:Edil3
Synonyms:Del1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1329025 Edil3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000752324 – 480EGF-like repeat and discoidin I-like domain-containing protein 3Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 37By similarity
Disulfide bondi31 ↔ 48By similarity
Disulfide bondi50 ↔ 59By similarity
Glycosylationi73O-linked (GalNAc...) threonineBy similarity1
Disulfide bondi78 ↔ 89By similarity
Disulfide bondi83 ↔ 105By similarity
Glycosylationi88O-linked (Fuc...) threonineBy similarity1
Disulfide bondi107 ↔ 116By similarity
Disulfide bondi123 ↔ 134By similarity
Disulfide bondi128 ↔ 143By similarity
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi145 ↔ 154By similarity
Disulfide bondi158 ↔ 314By similarity
Disulfide bondi301 ↔ 305By similarity
Disulfide bondi319 ↔ 476By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO35474
PaxDbiO35474
PeptideAtlasiO35474
PRIDEiO35474

PTM databases

PhosphoSitePlusiO35474

Expressioni

Tissue specificityi

Expressed in angioblasts and early endothelial cells. By embryonic day 13.5, also expressed in a restricted group of non-endothelial cells including chondrocytes and retinal neurons.

Developmental stagei

Expressed in the embryo from day 7. After day 15.5, expression decreases and disappears completely by the time of birth.

Gene expression databases

BgeeiENSMUSG00000034488 Expressed in 211 organ(s), highest expression level in brainstem
CleanExiMM_EDIL3
ExpressionAtlasiO35474 baseline and differential
GenevisibleiO35474 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080462

Structurei

3D structure databases

ProteinModelPortaliO35474
SMRiO35474
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 60EGF-like 1PROSITE-ProRule annotationAdd BLAST37
Domaini74 – 117EGF-like 2PROSITE-ProRule annotationAdd BLAST44
Domaini119 – 155EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini158 – 314F5/8 type C 1PROSITE-ProRule annotationAdd BLAST157
Domaini319 – 476F5/8 type C 2PROSITE-ProRule annotationAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 98Cell attachment siteBy similarity3

Domaini

EGF2 and EGF3 form a rigid rod via an interdomain calcium ion binding site, while the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1.By similarity

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFBC Eukaryota
ENOG41114BV LUCA
GeneTreeiENSGT00910000143988
HOGENOMiHOG000236278
HOVERGENiHBG002385
InParanoidiO35474
OrthoDBiEOG091G071G
TreeFamiTF330156

Family and domain databases

CDDicd00057 FA58C, 2 hits
Gene3Di2.60.120.260, 2 hits
InterProiView protein in InterPro
IPR029828 EDIL-3
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR000421 FA58C
IPR008979 Galactose-bd-like_sf
PANTHERiPTHR44122:SF3 PTHR44122:SF3, 1 hit
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00754 F5_F8_type_C, 2 hits
PF12661 hEGF, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 3 hits
SM00231 FA58C, 2 hits
SUPFAMiSSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS01187 EGF_CA, 1 hit
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O35474-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKHLVAAWLL VGLSLGVPQF GKGDICNPNP CENGGICLSG LADDSFSCEC
60 70 80 90 100
PEGFAGPNCS SVVEVASDEE KPTSAGPCIP NPCHNGGTCE ISEAYRGDTF
110 120 130 140 150
IGYVCKCPRG FNGIHCQHNI NECEAEPCRN GGICTDLVAN YSCECPGEFM
160 170 180 190 200
GRNCQYKCSG PLGIEGGIIS NQQITASSTH RALFGLQKWY PYYARLNKKG
210 220 230 240 250
LINAWTAAEN DRWPWIQINL QRKMRVTGVI TQGAKRIGSP EYIKSYKIAY
260 270 280 290 300
SNDGKTWAMY KVKGTNEEMV FRGNVDNNTP YANSFTPPIK AQYVRLYPQI
310 320 330 340 350
CRRHCTLRME LLGCELSGCS EPLGMKSGHI QDYQITASSV FRTLNMDMFT
360 370 380 390 400
WEPRKARLDK QGKVNAWTSG HNDQSQWLQV DLLVPTKVTG IITQGAKDFG
410 420 430 440 450
HVQFVGSYKL AYSNDGEHWM VHQDEKQRKD KVFQGNFDND THRKNVIDPP
460 470 480
IYARFIRILP WSWYGRITLR SELLGCAEEE
Length:480
Mass (Da):53,712
Last modified:July 27, 2011 - v2
Checksum:i10DBA65C19F1C70F
GO
Isoform 2 (identifier: O35474-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     218-221: INLQ → VTVG
     222-480: Missing.

Show »
Length:221
Mass (Da):23,751
Checksum:i45F77DB8EDABBDD7
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8C4U8Q8C4U8_MOUSE
EGF-like repeat and discoidin I-lik...
Edil3 mCG_142525
470Annotation score:
Q8C8K0Q8C8K0_MOUSE
EGF-like repeat and discoidin I-lik...
Edil3
384Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187Q → R in AAB86585 (PubMed:9420328).Curated1
Sequence conflicti187Q → R in AAB86586 (PubMed:9420328).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050400218 – 221INLQ → VTVG in isoform 2. 1 Publication4
Alternative sequenceiVSP_050401222 – 480Missing in isoform 2. 1 PublicationAdd BLAST259

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031524 mRNA Translation: AAB86585.1
AF031525 mRNA Translation: AAB86586.1
AK036117 mRNA Translation: BAC29310.1
AK139093 mRNA Translation: BAE23887.1
CT010458
, AC121916, AC154668, AC161244 Genomic DNA Translation: CAO91806.1
CH466567 Genomic DNA Translation: EDL00986.1
BC056386 mRNA Translation: AAH56386.1
CCDSiCCDS26669.1 [O35474-1]
RefSeqiNP_001033076.1, NM_001037987.3 [O35474-1]
NP_034233.1, NM_010103.4
UniGeneiMm.125580

Genome annotation databases

EnsembliENSMUST00000081769; ENSMUSP00000080462; ENSMUSG00000034488 [O35474-1]
GeneIDi13612
KEGGimmu:13612
UCSCiuc007rjb.2 mouse [O35474-2]
uc007rjd.2 mouse [O35474-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031524 mRNA Translation: AAB86585.1
AF031525 mRNA Translation: AAB86586.1
AK036117 mRNA Translation: BAC29310.1
AK139093 mRNA Translation: BAE23887.1
CT010458
, AC121916, AC154668, AC161244 Genomic DNA Translation: CAO91806.1
CH466567 Genomic DNA Translation: EDL00986.1
BC056386 mRNA Translation: AAH56386.1
CCDSiCCDS26669.1 [O35474-1]
RefSeqiNP_001033076.1, NM_001037987.3 [O35474-1]
NP_034233.1, NM_010103.4
UniGeneiMm.125580

3D structure databases

ProteinModelPortaliO35474
SMRiO35474
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080462

PTM databases

PhosphoSitePlusiO35474

Proteomic databases

MaxQBiO35474
PaxDbiO35474
PeptideAtlasiO35474
PRIDEiO35474

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081769; ENSMUSP00000080462; ENSMUSG00000034488 [O35474-1]
GeneIDi13612
KEGGimmu:13612
UCSCiuc007rjb.2 mouse [O35474-2]
uc007rjd.2 mouse [O35474-1]

Organism-specific databases

CTDi10085
MGIiMGI:1329025 Edil3

Phylogenomic databases

eggNOGiENOG410IFBC Eukaryota
ENOG41114BV LUCA
GeneTreeiENSGT00910000143988
HOGENOMiHOG000236278
HOVERGENiHBG002385
InParanoidiO35474
OrthoDBiEOG091G071G
TreeFamiTF330156

Miscellaneous databases

ChiTaRSiEdil3 mouse
PROiPR:O35474
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034488 Expressed in 211 organ(s), highest expression level in brainstem
CleanExiMM_EDIL3
ExpressionAtlasiO35474 baseline and differential
GenevisibleiO35474 MM

Family and domain databases

CDDicd00057 FA58C, 2 hits
Gene3Di2.60.120.260, 2 hits
InterProiView protein in InterPro
IPR029828 EDIL-3
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR000421 FA58C
IPR008979 Galactose-bd-like_sf
PANTHERiPTHR44122:SF3 PTHR44122:SF3, 1 hit
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00754 F5_F8_type_C, 2 hits
PF12661 hEGF, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 3 hits
SM00231 FA58C, 2 hits
SUPFAMiSSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS01187 EGF_CA, 1 hit
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEDIL3_MOUSE
AccessioniPrimary (citable) accession number: O35474
Secondary accession number(s): O35475, Q8CBF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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