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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6

Gene

Hsd3b6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. May be involved in local production of progesterone.

Catalytic activityi

A 3-beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH.
A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Oxidoreductase
Biological processSteroidogenesis
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis
UniPathwayi
UPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 (EC:1.1.1.-)
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type VI
Short name:
3-beta-HSD VI
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109598 Hsd3b6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877851 – 3733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6Add BLAST373

Proteomic databases

MaxQBiO35469
PaxDbiO35469
PeptideAtlasiO35469
PRIDEiO35469

PTM databases

iPTMnetiO35469
PhosphoSitePlusiO35469

Expressioni

Tissue specificityi

Expressed in skin and testis.

Developmental stagei

Earliest isoform to be expressed during embryogenesis in cells of embryonic origin at 7 and 9.5 dpc, and is the major isoform expressed in uterine tissue at the time of implantation (4.5 dpc) and continues to be expressed in uterine tissue at 6.5, 7.5 and 9.5 dpc. It is expressed in giant trophoblasts at 9.5 dpc and is expressed in the placenta through 15.5 dpc.

Gene expression databases

BgeeiENSMUSG00000027869 Expressed in 49 organ(s), highest expression level in testis
GenevisibleiO35469 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029463

Structurei

3D structure databases

ProteinModelPortaliO35469
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiO35469
KOiK00070
OMAiYSYQPPF
OrthoDBiEOG091G09QZ
TreeFamiTF343138

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

O35469-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGWSCLVTG AGGFLGQRIV QLLMQEKDLE EIRVLDKFFR PETREQFFNL
60 70 80 90 100
DTNIKVTVLE GDILDTQYLR KACQGISVVI HTAAVIDVTG VIPRQTILDV
110 120 130 140 150
NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKEIILN GNEEEHHESI
160 170 180 190 200
WSDPYPYSKK MAEKAVLAAN GSMLKIGGTL HTCALRPMYI YGERSPFISN
210 220 230 240 250
TIITALKNKN ILGCTGKFST ANPVYVGNVA WAHILAARGL RDPKKSPNIQ
260 270 280 290 300
GEFYYISDDT PHQSYDDLNY TLSKEWGFCP DSSWSLPVPL LYWLAFMLET
310 320 330 340 350
VSFLLSPIYR FIPPFNRHLV TLTGSTFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):41,977
Last modified:June 28, 2011 - v4
Checksum:i10FE9278C5BDC534
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189Y → F in AAB84299 (PubMed:9075693).Curated1
Sequence conflicti366E → A in AAB84299 (PubMed:9075693).Curated1
Sequence conflicti371K → T in AAB84299 (PubMed:9075693).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031170 mRNA Translation: AAB84299.1
AK142267 mRNA Translation: BAE25002.1
AL606755 Genomic DNA Translation: CAM13693.1
CH466620 Genomic DNA Translation: EDL38971.1
CCDSiCCDS17669.1
RefSeqiNP_038849.2, NM_013821.3
UniGeneiMm.14435

Genome annotation databases

EnsembliENSMUST00000029463; ENSMUSP00000029463; ENSMUSG00000027869
ENSMUST00000170847; ENSMUSP00000129911; ENSMUSG00000027869
GeneIDi15497
KEGGimmu:15497
UCSCiuc012cuq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031170 mRNA Translation: AAB84299.1
AK142267 mRNA Translation: BAE25002.1
AL606755 Genomic DNA Translation: CAM13693.1
CH466620 Genomic DNA Translation: EDL38971.1
CCDSiCCDS17669.1
RefSeqiNP_038849.2, NM_013821.3
UniGeneiMm.14435

3D structure databases

ProteinModelPortaliO35469
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029463

PTM databases

iPTMnetiO35469
PhosphoSitePlusiO35469

Proteomic databases

MaxQBiO35469
PaxDbiO35469
PeptideAtlasiO35469
PRIDEiO35469

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029463; ENSMUSP00000029463; ENSMUSG00000027869
ENSMUST00000170847; ENSMUSP00000129911; ENSMUSG00000027869
GeneIDi15497
KEGGimmu:15497
UCSCiuc012cuq.1 mouse

Organism-specific databases

CTDi15497
MGIiMGI:109598 Hsd3b6

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiO35469
KOiK00070
OMAiYSYQPPF
OrthoDBiEOG091G09QZ
TreeFamiTF343138

Enzyme and pathway databases

UniPathwayi
UPA00062

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis

Miscellaneous databases

PROiPR:O35469
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027869 Expressed in 49 organ(s), highest expression level in testis
GenevisibleiO35469 MM

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3BHS6_MOUSE
AccessioniPrimary (citable) accession number: O35469
Secondary accession number(s): Q3UQN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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