UniProtKB - O35459 (ECH1_MOUSE)
Protein
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Gene
Ech1
Organism
Mus musculus (Mouse)
Status
Functioni
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.By similarity
Catalytic activityi
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 173 | Substrate; via amide nitrogenBy similarity | 1 | |
Sitei | 196 | Important for catalytic activityBy similarity | 1 | |
Sitei | 204 | Important for catalytic activityBy similarity | 1 |
GO - Molecular functioni
- delta3,5-delta2,4-dienoyl-CoA isomerase activity Source: GO_Central
GO - Biological processi
- fatty acid beta-oxidation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-9033241, Peroxisomal protein import |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialCurated (EC:5.3.3.-By similarity) |
Gene namesi | Name:Ech1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858208, Ech1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Peroxisome
- Peroxisome By similarity
Mitochondrion
- mitochondrion Source: MGI
Peroxisome
- peroxisome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Mitochondrion, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 33 | MitochondrionSequence analysisAdd BLAST | 33 | |
ChainiPRO_0000007418 | 34 – 327 | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialAdd BLAST | 294 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 147 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 230 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 267 | PhosphoserineBy similarity | 1 | |
Modified residuei | 316 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 326 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O35459 |
jPOSTi | O35459 |
MaxQBi | O35459 |
PaxDbi | O35459 |
PeptideAtlasi | O35459 |
PRIDEi | O35459 |
2D gel databases
REPRODUCTION-2DPAGEi | O35459 |
PTM databases
iPTMneti | O35459 |
PhosphoSitePlusi | O35459 |
SwissPalmi | O35459 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000053898, Expressed in brown adipose tissue and 307 other tissues |
Genevisiblei | O35459, MM |
Interactioni
Subunit structurei
Homohexamer.
By similarityProtein-protein interaction databases
BioGRIDi | 206181, 34 interactors |
IntActi | O35459, 6 interactors |
MINTi | O35459 |
STRINGi | 10090.ENSMUSP00000066092 |
Miscellaneous databases
RNActi | O35459, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 119 | Substrate bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 325 – 327 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the enoyl-CoA hydratase/isomerase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1681, Eukaryota |
GeneTreei | ENSGT00940000159610 |
InParanoidi | O35459 |
OMAi | HKACIGA |
OrthoDBi | 1094098at2759 |
PhylomeDBi | O35459 |
TreeFami | TF314317 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O35459-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS
60 70 80 90 100
DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD
110 120 130 140 150
CRAVVVSGAG KMFTSGIDLM DMASELMQPS GDDAARIAWY LRDLISKYQK
160 170 180 190 200
TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA CDIRYCTQDA FFQIKEVDMG
210 220 230 240 250
LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG LVSRVFQDKD
260 270 280 290 300
AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM
310 320
LQTQDIIKSV QAAMEKRDTK SITFSKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7B227 | F7B227_MOUSE | Delta(3,5)-Delta(2,4)-dienoyl-CoA i... | Ech1 | 177 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030343 mRNA Translation: AAB84224.1 BC068112 mRNA Translation: AAH68112.1 BC087924 mRNA Translation: AAH87924.1 |
CCDSi | CCDS21057.1 |
RefSeqi | NP_058052.1, NM_016772.1 |
Genome annotation databases
Ensembli | ENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898 |
GeneIDi | 51798 |
KEGGi | mmu:51798 |
UCSCi | uc009gab.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030343 mRNA Translation: AAB84224.1 BC068112 mRNA Translation: AAH68112.1 BC087924 mRNA Translation: AAH87924.1 |
CCDSi | CCDS21057.1 |
RefSeqi | NP_058052.1, NM_016772.1 |
3D structure databases
SMRi | O35459 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206181, 34 interactors |
IntActi | O35459, 6 interactors |
MINTi | O35459 |
STRINGi | 10090.ENSMUSP00000066092 |
PTM databases
iPTMneti | O35459 |
PhosphoSitePlusi | O35459 |
SwissPalmi | O35459 |
2D gel databases
REPRODUCTION-2DPAGEi | O35459 |
Proteomic databases
EPDi | O35459 |
jPOSTi | O35459 |
MaxQBi | O35459 |
PaxDbi | O35459 |
PeptideAtlasi | O35459 |
PRIDEi | O35459 |
Protocols and materials databases
Antibodypediai | 1042, 264 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000066264; ENSMUSP00000066092; ENSMUSG00000053898 |
GeneIDi | 51798 |
KEGGi | mmu:51798 |
UCSCi | uc009gab.1, mouse |
Organism-specific databases
CTDi | 1891 |
MGIi | MGI:1858208, Ech1 |
Phylogenomic databases
eggNOGi | KOG1681, Eukaryota |
GeneTreei | ENSGT00940000159610 |
InParanoidi | O35459 |
OMAi | HKACIGA |
OrthoDBi | 1094098at2759 |
PhylomeDBi | O35459 |
TreeFami | TF314317 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Reactomei | R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 51798, 0 hits in 17 CRISPR screens |
ChiTaRSi | Ech1, mouse |
PROi | PR:O35459 |
RNActi | O35459, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000053898, Expressed in brown adipose tissue and 307 other tissues |
Genevisiblei | O35459, MM |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ECH1_MOUSE | |
Accessioni | O35459Primary (citable) accession number: O35459 Secondary accession number(s): Q5M8P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families