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Protein

Lysosomal thioesterase PPT2

Gene

Ppt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei111NucleophileBy similarity1
Active sitei228By similarity1
Active sitei283By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-75105 Fatty acyl-CoA biosynthesis

Protein family/group databases

ESTHERimouse-PPT2 Palmitoyl-protein_thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal thioesterase PPT2 (EC:3.1.2.-)
Short name:
PPT-2
Gene namesi
Name:Ppt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1860075 Ppt2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Mice are healthy at birth, but develop neuronal abnormalities, infiltration of bone marrow by macrophages and multinucleated giant cells, and splenomegaly caused by extramedullary hematopoiesis. Autofluorescent storage material is present in many cell types, particularly reticuloendothelial cells and neurons.2 Publications

Chemistry databases

ChEMBLiCHEMBL3259496

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000002555528 – 302Lysosomal thioesterase PPT2Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi109 ↔ 117By similarity
Disulfide bondi165 ↔ 176By similarity
Glycosylationi190N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi245N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi276 ↔ 296By similarity
Glycosylationi289N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO35448
MaxQBiO35448
PaxDbiO35448
PeptideAtlasiO35448
PRIDEiO35448

Expressioni

Tissue specificityi

Expressed throughout the brain, primarily in neurons, and at lower levels in glial cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000015474 Expressed in 276 organ(s), highest expression level in ear vesicle
CleanExiMM_PPT2
ExpressionAtlasiO35448 baseline and differential
GenevisibleiO35448 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068071

Structurei

3D structure databases

ProteinModelPortaliO35448
SMRiO35448
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
GeneTreeiENSGT00530000063368
HOGENOMiHOG000264240
HOVERGENiHBG019159
InParanoidiO35448
KOiK01074
OMAiAWHTRRD
OrthoDBiEOG091G0CYO
PhylomeDBiO35448
TreeFamiTF323926

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030295 PPT2
PANTHERiPTHR11247:SF27 PTHR11247:SF27, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O35448-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGLWRQRLP SAWALLLLPF LPLLMPAAPA AHRGSYKPVI VVHGLFDSSY
60 70 80 90 100
SFRHLLDYIN ETHTGTVVTV LDLFDGRESL RPLWEQVQGF REAVVPIMEK
110 120 130 140 150
APEGVHLICY SQGGLVCRAL LSVMDNHNVD SFISLSSPQM GQYGDTDYLK
160 170 180 190 200
WLFPTSMRSN LYRVCYSPWG QEFSICNYWH DPHHDDLYLN ASSFLALING
210 220 230 240 250
ERDHPNATAW RKNFLRVGRL VLIGGPDDGV ITPWQSSFFG FYDANETVLE
260 270 280 290 300
MEEQPVYLRD SFGLKTLLAR GAIVRCPMAG ISHTTWHSNR TLYDTCIEPW

LS
Length:302
Mass (Da):34,366
Last modified:January 1, 1998 - v1
Checksum:i3F1F698400495655
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0T0E9Q0T0_MOUSE
Lysosomal thioesterase PPT2
Ppt2
203Annotation score:
E9PVM9E9PVM9_MOUSE
Lysosomal thioesterase PPT2
Ppt2
102Annotation score:
G5E8W7G5E8W7_MOUSE
Lysosomal thioesterase PPT2
Ppt2
236Annotation score:
F6SPQ1F6SPQ1_MOUSE
Lysosomal thioesterase PPT2
Ppt2
247Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA Translation: AAB82011.1
BC013462 mRNA Translation: AAH13462.1
BC052330 mRNA Translation: AAH52330.1
CCDSiCCDS28652.1
PIRiT09066
RefSeqiNP_001289322.1, NM_001302393.1
NP_001289323.1, NM_001302394.1
NP_001289324.1, NM_001302395.1
NP_001289325.1, NM_001302396.1
NP_062314.1, NM_019441.5
XP_006524701.1, XM_006524638.2
UniGeneiMm.373627
Mm.486254

Genome annotation databases

EnsembliENSMUST00000064953; ENSMUSP00000068071; ENSMUSG00000015474
ENSMUST00000166040; ENSMUSP00000132006; ENSMUSG00000015474
ENSMUST00000168391; ENSMUSP00000132339; ENSMUSG00000015474
ENSMUST00000169067; ENSMUSP00000127372; ENSMUSG00000015474
ENSMUST00000171121; ENSMUSP00000127745; ENSMUSG00000015474
ENSMUST00000171376; ENSMUSP00000131243; ENSMUSG00000015474
GeneIDi54397
KEGGimmu:54397
UCSCiuc008cdd.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA Translation: AAB82011.1
BC013462 mRNA Translation: AAH13462.1
BC052330 mRNA Translation: AAH52330.1
CCDSiCCDS28652.1
PIRiT09066
RefSeqiNP_001289322.1, NM_001302393.1
NP_001289323.1, NM_001302394.1
NP_001289324.1, NM_001302395.1
NP_001289325.1, NM_001302396.1
NP_062314.1, NM_019441.5
XP_006524701.1, XM_006524638.2
UniGeneiMm.373627
Mm.486254

3D structure databases

ProteinModelPortaliO35448
SMRiO35448
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068071

Chemistry databases

ChEMBLiCHEMBL3259496

Protein family/group databases

ESTHERimouse-PPT2 Palmitoyl-protein_thioesterase

Proteomic databases

EPDiO35448
MaxQBiO35448
PaxDbiO35448
PeptideAtlasiO35448
PRIDEiO35448

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064953; ENSMUSP00000068071; ENSMUSG00000015474
ENSMUST00000166040; ENSMUSP00000132006; ENSMUSG00000015474
ENSMUST00000168391; ENSMUSP00000132339; ENSMUSG00000015474
ENSMUST00000169067; ENSMUSP00000127372; ENSMUSG00000015474
ENSMUST00000171121; ENSMUSP00000127745; ENSMUSG00000015474
ENSMUST00000171376; ENSMUSP00000131243; ENSMUSG00000015474
GeneIDi54397
KEGGimmu:54397
UCSCiuc008cdd.2 mouse

Organism-specific databases

CTDi9374
MGIiMGI:1860075 Ppt2

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
GeneTreeiENSGT00530000063368
HOGENOMiHOG000264240
HOVERGENiHBG019159
InParanoidiO35448
KOiK01074
OMAiAWHTRRD
OrthoDBiEOG091G0CYO
PhylomeDBiO35448
TreeFamiTF323926

Enzyme and pathway databases

ReactomeiR-MMU-75105 Fatty acyl-CoA biosynthesis

Miscellaneous databases

ChiTaRSiPpt2 mouse
PROiPR:O35448
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015474 Expressed in 276 organ(s), highest expression level in ear vesicle
CleanExiMM_PPT2
ExpressionAtlasiO35448 baseline and differential
GenevisibleiO35448 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030295 PPT2
PANTHERiPTHR11247:SF27 PTHR11247:SF27, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPT2_MOUSE
AccessioniPrimary (citable) accession number: O35448
Secondary accession number(s): Q80WP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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