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Protein

E3 ubiquitin-protein ligase RNF5

Gene

Rnf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150 'leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport
R-MMU-901032 ER Quality Control Compartment (ERQC)
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF5 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 5
RING-type E3 ubiquitin transferase RNF5Curated
Gene namesi
Name:Rnf5
Synonyms:Ng2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1860076 Rnf5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002403942 – 180E3 ubiquitin-protein ligase RNF5Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei84PhosphoserineBy similarity1
Modified residuei94PhosphothreonineBy similarity1
Modified residuei107PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35445
PaxDbiO35445
PeptideAtlasiO35445
PRIDEiO35445

PTM databases

iPTMnetiO35445
PhosphoSitePlusiO35445

Expressioni

Gene expression databases

BgeeiENSMUSG00000015478 Expressed in 286 organ(s), highest expression level in neocortex
CleanExiMM_RNF5
GenevisibleiO35445 MM

Interactioni

Subunit structurei

Interacts with PXN (By similarity). Interacts with JKAMP. Interacts with TMEM173; the interaction of endogenous proteins is dependent on viral infection (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207597, 1 interactor
MINTiO35445
STRINGi10090.ENSMUSP00000015622

Structurei

3D structure databases

ProteinModelPortaliO35445
SMRiO35445
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823 Eukaryota
ENOG4111IHV LUCA
GeneTreeiENSGT00390000014107
HOGENOMiHOG000238304
HOVERGENiHBG054495
InParanoidiO35445
KOiK10666
OMAiYAGDHQG
OrthoDBiEOG091G0W2I
PhylomeDBiO35445
TreeFamiTF317334

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35445-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAEEEDGG PEGPNRERGG ASATFECNIC LETAREAVVS VCGHLYCWPC
60 70 80 90 100
LHQWLETRPD RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG
110 120 130 140 150
QRPAPESRGG FQPFGDAGGF HFSFGVGAFP FGFFTTVFNA HEPFRRGAGV
160 170 180
DLGQGHPASS WQDSLFLFLA IFFFFWLLSI
Length:180
Mass (Da):19,837
Last modified:January 1, 1998 - v1
Checksum:i3C00A30201F590CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA Translation: AAB82008.1
BC016449 mRNA Translation: AAH16449.1
CCDSiCCDS28650.1
PIRiT09063
RefSeqiNP_062276.1, NM_019403.3
UniGeneiMm.274542

Genome annotation databases

EnsembliENSMUST00000015622; ENSMUSP00000015622; ENSMUSG00000015478
GeneIDi54197
KEGGimmu:54197
UCSCiuc008ccy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030001 Genomic DNA Translation: AAB82008.1
BC016449 mRNA Translation: AAH16449.1
CCDSiCCDS28650.1
PIRiT09063
RefSeqiNP_062276.1, NM_019403.3
UniGeneiMm.274542

3D structure databases

ProteinModelPortaliO35445
SMRiO35445
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207597, 1 interactor
MINTiO35445
STRINGi10090.ENSMUSP00000015622

PTM databases

iPTMnetiO35445
PhosphoSitePlusiO35445

Proteomic databases

MaxQBiO35445
PaxDbiO35445
PeptideAtlasiO35445
PRIDEiO35445

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015622; ENSMUSP00000015622; ENSMUSG00000015478
GeneIDi54197
KEGGimmu:54197
UCSCiuc008ccy.1 mouse

Organism-specific databases

CTDi6048
MGIiMGI:1860076 Rnf5

Phylogenomic databases

eggNOGiKOG0823 Eukaryota
ENOG4111IHV LUCA
GeneTreeiENSGT00390000014107
HOGENOMiHOG000238304
HOVERGENiHBG054495
InParanoidiO35445
KOiK10666
OMAiYAGDHQG
OrthoDBiEOG091G0W2I
PhylomeDBiO35445
TreeFamiTF317334

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-382556 ABC-family proteins mediated transport
R-MMU-901032 ER Quality Control Compartment (ERQC)

Miscellaneous databases

PROiPR:O35445
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015478 Expressed in 286 organ(s), highest expression level in neocortex
CleanExiMM_RNF5
GenevisibleiO35445 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF5_MOUSE
AccessioniPrimary (citable) accession number: O35445
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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