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Protein

HCLS1-associated protein X-1

Gene

Hax1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
HCLS1-associated protein X-1
Alternative name(s):
HS1-associating protein X-1
Short name:
HAX-1
HS1-binding protein 1
Short name:
HSP1BP-1
Gene namesi
Name:Hax1
Synonyms:Hs1bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1346319 Hax1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Mice lacking Hax1 fail to survive longer than 14 weeks, due to a loss of motor coordination and activity, leading to failure to eat and drink. They display extensive apoptosis of neurons in the striatum and cerebellum, and a loss of lymphocytes in spleen, bone marrow and thymus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000839072 – 280HCLS1-associated protein X-1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei193PhosphoserineBy similarity1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei128 – 129Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35387
MaxQBiO35387
PaxDbiO35387
PRIDEiO35387

PTM databases

iPTMnetiO35387
PhosphoSitePlusiO35387

Expressioni

Tissue specificityi

Ubiquitous, with highest levels in kidney and liver (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027944 Expressed in 21 organ(s), highest expression level in female gonad
CleanExiMM_HAX1
ExpressionAtlasiO35387 baseline and differential
GenevisibleiO35387 MM

Interactioni

Subunit structurei

Interacts with ABCB1, ABCB4 and ABCB11 (By similarity). Directly associates with HCLS1/HS1, through binding to its N-terminal region (By similarity). Interacts with CTTN (PubMed:10760273). Interacts with PKD2(PubMed:10760273). Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with TPC2. Interacts with KCNC3 (PubMed:26997484). Interacts with XPO1 (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204783, 16 interactors
CORUMiO35387
DIPiDIP-49445N
IntActiO35387, 21 interactors
STRINGi10090.ENSMUSP00000078661

Structurei

3D structure databases

ProteinModelPortaliO35387
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 45Required for localization in mitochondriaBy similarityAdd BLAST44
Regioni115 – 280Involved in HCLS1 bindingBy similarityAdd BLAST166
Regioni176 – 207Involved in CASP9 bindingBy similarityAdd BLAST32
Regioni177 – 248Involved in GNA13 bindingBy similarityAdd BLAST72
Regioni184 – 280Required for localization in sarcoplasmic reticulumBy similarityAdd BLAST97
Regioni185 – 280Involved in PKD2 bindingAdd BLAST96
Regioni204 – 246Involved in ATP2A2 bindingBy similarityAdd BLAST43
Regioni204 – 226Involved in PLN bindingBy similarityAdd BLAST23
Regioni271 – 280Required for ITGB6 bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 44Asp/Glu-rich (highly acidic)Add BLAST15

Sequence similaritiesi

Belongs to the HAX1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJQ0 Eukaryota
ENOG411211U LUCA
GeneTreeiENSGT00390000018324
HOGENOMiHOG000090247
HOVERGENiHBG002991
InParanoidiO35387
KOiK16220
OMAiGSQRPFH
OrthoDBiEOG091G0MK9
PhylomeDBiO35387
TreeFamiTF328619

Family and domain databases

InterProiView protein in InterPro
IPR017248 HAX-1
PANTHERiPTHR14938 PTHR14938, 1 hit
PIRSFiPIRSF037634 HS1-associating_X-1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

O35387-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDDDDDDE AEEDRGAWGR
60 70 80 90 100
ESYAFDGSQP PEEFGFSFSP RGGMRFHGNF GFDDLVRDFN SIFSEMGAWT
110 120 130 140 150
LPSHSPELPG PESETPGERL REGQTLRDSM LKYPDSHQPR IFEGVLESHA
160 170 180 190 200
KPESPKPAPD WGSQGPFHRL DDTWPVSPHS RAKEDKDLDS QVSQEGLGPL
210 220 230 240 250
LQPQPKSYFK SISVTKITKP DGTVEERRTV VDSEGRRETT VTHQEAHDSS
260 270 280
RSDPDSQRSS ALDDPFSILD LLLGRWFRSR
Length:280
Mass (Da):31,654
Last modified:January 1, 1998 - v1
Checksum:i3123979BA10B45D1
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JDP0A0A0G2JDP0_MOUSE
HCLS1-associated protein X-1
Hax1
277Annotation score:
A0A0G2JFF9A0A0G2JFF9_MOUSE
HCLS1-associated protein X-1
Hax1
254Annotation score:
A0A0G2JE46A0A0G2JE46_MOUSE
HCLS1-associated protein X-1
Hax1 mCG_124547
196Annotation score:
A0A0G2JGG5A0A0G2JGG5_MOUSE
HCLS1-associated protein X-1
Hax1
69Annotation score:
A0A0G2JEE6A0A0G2JEE6_MOUSE
HCLS1-associated protein X-1
Hax1
151Annotation score:
A0A0G2JFE1A0A0G2JFE1_MOUSE
HCLS1-associated protein X-1
Hax1
66Annotation score:
A0A0G2JGR0A0A0G2JGR0_MOUSE
HCLS1-associated protein X-1
Hax1
36Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023482 mRNA Translation: AAB81081.1
AK002256 mRNA Translation: BAB21969.1
AK010633 mRNA Translation: BAB27077.1
AK088746 mRNA Translation: BAC40544.1
BC006688 mRNA Translation: AAH06688.1
BC098225 mRNA Translation: AAH98225.1
CCDSiCCDS17519.1
RefSeqiNP_035956.1, NM_011826.4
UniGeneiMm.256035
Mm.416677

Genome annotation databases

EnsembliENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944
GeneIDi23897
KEGGimmu:23897
UCSCiuc008qaj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023482 mRNA Translation: AAB81081.1
AK002256 mRNA Translation: BAB21969.1
AK010633 mRNA Translation: BAB27077.1
AK088746 mRNA Translation: BAC40544.1
BC006688 mRNA Translation: AAH06688.1
BC098225 mRNA Translation: AAH98225.1
CCDSiCCDS17519.1
RefSeqiNP_035956.1, NM_011826.4
UniGeneiMm.256035
Mm.416677

3D structure databases

ProteinModelPortaliO35387
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204783, 16 interactors
CORUMiO35387
DIPiDIP-49445N
IntActiO35387, 21 interactors
STRINGi10090.ENSMUSP00000078661

PTM databases

iPTMnetiO35387
PhosphoSitePlusiO35387

Proteomic databases

EPDiO35387
MaxQBiO35387
PaxDbiO35387
PRIDEiO35387

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944
GeneIDi23897
KEGGimmu:23897
UCSCiuc008qaj.2 mouse

Organism-specific databases

CTDi10456
MGIiMGI:1346319 Hax1

Phylogenomic databases

eggNOGiENOG410IJQ0 Eukaryota
ENOG411211U LUCA
GeneTreeiENSGT00390000018324
HOGENOMiHOG000090247
HOVERGENiHBG002991
InParanoidiO35387
KOiK16220
OMAiGSQRPFH
OrthoDBiEOG091G0MK9
PhylomeDBiO35387
TreeFamiTF328619

Miscellaneous databases

PROiPR:O35387
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027944 Expressed in 21 organ(s), highest expression level in female gonad
CleanExiMM_HAX1
ExpressionAtlasiO35387 baseline and differential
GenevisibleiO35387 MM

Family and domain databases

InterProiView protein in InterPro
IPR017248 HAX-1
PANTHERiPTHR14938 PTHR14938, 1 hit
PIRSFiPIRSF037634 HS1-associating_X-1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHAX1_MOUSE
AccessioniPrimary (citable) accession number: O35387
Secondary accession number(s): Q542F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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