UniProtKB - O35387 (HAX1_MOUSE)
HCLS1-associated protein X-1
Hax1
Functioni
Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
By similarityGO - Molecular functioni
- interleukin-1 binding Source: MGI
- protein domain specific binding Source: MGI
- protein N-terminus binding Source: MGI
GO - Biological processi
- cell surface receptor signaling pathway Source: MGI
- cellular response to cytokine stimulus Source: MGI
- negative regulation of apoptotic process Source: MGI
- positive regulation of actin cytoskeleton reorganization Source: MGI
- positive regulation of granulocyte differentiation Source: MGI
- positive regulation of peptidyl-serine phosphorylation Source: MGI
- positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
- positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of actin filament polymerization Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: HCLS1-associated protein X-1Alternative name(s): HS1-associating protein X-1 Short name: HAX-1 HS1-binding protein 1 Short name: HSP1BP-1 |
Gene namesi | Name:Hax1 Synonyms:Hs1bp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346319, Hax1 |
VEuPathDBi | HostDB:ENSMUSG00000027944 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus membrane 1 Publication
- Nucleus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Other locations
- Cytoplasmic vesicle 2 Publications
- cell cortex By similarity
- Sarcoplasmic reticulum By similarity
- P-body By similarity
Note: Predominantly cytoplasmic. Also detected in the nucleus when nuclear export is inhibited (in vitro).By similarity
Cytoskeleton
- actin cytoskeleton Source: BHF-UCL
Endoplasmic reticulum
- endoplasmic reticulum Source: BHF-UCL
- sarcoplasmic reticulum Source: CACAO
Mitochondrion
- mitochondrial intermembrane space Source: MGI
- mitochondrial outer membrane Source: MGI
- mitochondrion Source: MGI
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
Plasma Membrane
- apical plasma membrane Source: MGI
Other locations
- cell cortex Source: UniProtKB-SubCell
- clathrin-coated vesicle Source: MGI
- cytoplasm Source: BHF-UCL
- intracellular membrane-bounded organelle Source: MGI
- lamellipodium Source: BHF-UCL
- P-body Source: UniProtKB-SubCell
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulumPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000083907 | 2 – 280 | HCLS1-associated protein X-1Add BLAST | 279 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 190 | PhosphoserineBy similarity | 1 | |
Modified residuei | 193 | PhosphoserineBy similarity | 1 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 128 – 129 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O35387 |
jPOSTi | O35387 |
MaxQBi | O35387 |
PaxDbi | O35387 |
PRIDEi | O35387 |
ProteomicsDBi | 269814 |
PTM databases
iPTMneti | O35387 |
PhosphoSitePlusi | O35387 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000027944, Expressed in liver and 73 other tissues |
Genevisiblei | O35387, MM |
Interactioni
Subunit structurei
Interacts with ABCB1, ABCB4 and ABCB11 (By similarity). Directly associates with HCLS1/HS1, through binding to its N-terminal region (By similarity).
Interacts with CTTN (PubMed:10760273).
Interacts with PKD2(PubMed:10760273).
Interacts with GNA13.
Interacts with CASP9.
Interacts with ITGB6.
Interacts with PLN and ATP2A2; these interactions are inhibited by calcium.
Interacts with GRB7.
Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4.
Interacts with TPC2.
Interacts with KCNC3 (PubMed:26997484).
Interacts with XPO1 (By similarity).
Interacts with RNF217 (By similarity).
By similarity1 PublicationBinary interactionsi
O35387
GO - Molecular functioni
- interleukin-1 binding Source: MGI
- protein domain specific binding Source: MGI
- protein N-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204783, 17 interactors |
CORUMi | O35387 |
DIPi | DIP-49445N |
IntActi | O35387, 22 interactors |
MINTi | O35387 |
STRINGi | 10090.ENSMUSP00000078661 |
Miscellaneous databases
RNActi | O35387, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 45 | Required for localization in mitochondriaBy similarityAdd BLAST | 44 | |
Regioni | 12 – 70 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 100 – 263 | DisorderedSequence analysisAdd BLAST | 164 | |
Regioni | 115 – 280 | Involved in HCLS1 bindingBy similarityAdd BLAST | 166 | |
Regioni | 176 – 207 | Involved in CASP9 bindingBy similarityAdd BLAST | 32 | |
Regioni | 177 – 248 | Involved in GNA13 bindingBy similarityAdd BLAST | 72 | |
Regioni | 184 – 280 | Required for localization in sarcoplasmic reticulumBy similarityAdd BLAST | 97 | |
Regioni | 185 – 280 | Involved in PKD2 bindingAdd BLAST | 96 | |
Regioni | 204 – 246 | Involved in ATP2A2 bindingBy similarityAdd BLAST | 43 | |
Regioni | 204 – 226 | Involved in PLN bindingBy similarityAdd BLAST | 23 | |
Regioni | 271 – 280 | Required for ITGB6 bindingBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 28 – 44 | Acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 114 – 128 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 174 – 191 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 195 – 218 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 220 – 258 | Basic and acidic residuesSequence analysisAdd BLAST | 39 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S0AE, Eukaryota |
GeneTreei | ENSGT00390000018324 |
InParanoidi | O35387 |
OMAi | SKFNDIW |
OrthoDBi | 1567048at2759 |
PhylomeDBi | O35387 |
TreeFami | TF328619 |
Family and domain databases
InterProi | View protein in InterPro IPR017248, HAX-1 |
PANTHERi | PTHR14938, PTHR14938, 1 hit |
PIRSFi | PIRSF037634, HS1-associating_X-1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDDDDDDE AEEDRGAWGR
60 70 80 90 100
ESYAFDGSQP PEEFGFSFSP RGGMRFHGNF GFDDLVRDFN SIFSEMGAWT
110 120 130 140 150
LPSHSPELPG PESETPGERL REGQTLRDSM LKYPDSHQPR IFEGVLESHA
160 170 180 190 200
KPESPKPAPD WGSQGPFHRL DDTWPVSPHS RAKEDKDLDS QVSQEGLGPL
210 220 230 240 250
LQPQPKSYFK SISVTKITKP DGTVEERRTV VDSEGRRETT VTHQEAHDSS
260 270 280
RSDPDSQRSS ALDDPFSILD LLLGRWFRSR
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JFF9 | A0A0G2JFF9_MOUSE | HCLS1-associated protein X-1 | Hax1 | 254 | Annotation score: | ||
A0A0G2JDP0 | A0A0G2JDP0_MOUSE | HCLS1-associated protein X-1 | Hax1 | 277 | Annotation score: | ||
A0A0G2JE46 | A0A0G2JE46_MOUSE | HCLS1-associated protein X-1 | Hax1 | 196 | Annotation score: | ||
A0A0G2JEE6 | A0A0G2JEE6_MOUSE | HCLS1-associated protein X-1 | Hax1 | 151 | Annotation score: | ||
A0A0G2JFE1 | A0A0G2JFE1_MOUSE | HCLS1-associated protein X-1 | Hax1 | 66 | Annotation score: | ||
A0A0G2JGG5 | A0A0G2JGG5_MOUSE | HCLS1-associated protein X-1 | Hax1 | 69 | Annotation score: | ||
A0A0G2JGR0 | A0A0G2JGR0_MOUSE | HCLS1-associated protein X-1 | Hax1 | 36 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF023482 mRNA Translation: AAB81081.1 AK002256 mRNA Translation: BAB21969.1 AK010633 mRNA Translation: BAB27077.1 AK088746 mRNA Translation: BAC40544.1 BC006688 mRNA Translation: AAH06688.1 BC098225 mRNA Translation: AAH98225.1 |
CCDSi | CCDS17519.1 |
RefSeqi | NP_035956.1, NM_011826.4 |
Genome annotation databases
Ensembli | ENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944 |
GeneIDi | 23897 |
KEGGi | mmu:23897 |
UCSCi | uc008qaj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF023482 mRNA Translation: AAB81081.1 AK002256 mRNA Translation: BAB21969.1 AK010633 mRNA Translation: BAB27077.1 AK088746 mRNA Translation: BAC40544.1 BC006688 mRNA Translation: AAH06688.1 BC098225 mRNA Translation: AAH98225.1 |
CCDSi | CCDS17519.1 |
RefSeqi | NP_035956.1, NM_011826.4 |
3D structure databases
SMRi | O35387 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204783, 17 interactors |
CORUMi | O35387 |
DIPi | DIP-49445N |
IntActi | O35387, 22 interactors |
MINTi | O35387 |
STRINGi | 10090.ENSMUSP00000078661 |
PTM databases
iPTMneti | O35387 |
PhosphoSitePlusi | O35387 |
Proteomic databases
EPDi | O35387 |
jPOSTi | O35387 |
MaxQBi | O35387 |
PaxDbi | O35387 |
PRIDEi | O35387 |
ProteomicsDBi | 269814 |
Protocols and materials databases
Antibodypediai | 34150, 499 antibodies from 38 providers |
DNASUi | 23897 |
Genome annotation databases
Ensembli | ENSMUST00000079724; ENSMUSP00000078661; ENSMUSG00000027944 |
GeneIDi | 23897 |
KEGGi | mmu:23897 |
UCSCi | uc008qaj.2, mouse |
Organism-specific databases
CTDi | 10456 |
MGIi | MGI:1346319, Hax1 |
VEuPathDBi | HostDB:ENSMUSG00000027944 |
Phylogenomic databases
eggNOGi | ENOG502S0AE, Eukaryota |
GeneTreei | ENSGT00390000018324 |
InParanoidi | O35387 |
OMAi | SKFNDIW |
OrthoDBi | 1567048at2759 |
PhylomeDBi | O35387 |
TreeFami | TF328619 |
Miscellaneous databases
BioGRID-ORCSi | 23897, 2 hits in 63 CRISPR screens |
ChiTaRSi | Hax1, mouse |
PROi | PR:O35387 |
RNActi | O35387, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027944, Expressed in liver and 73 other tissues |
Genevisiblei | O35387, MM |
Family and domain databases
InterProi | View protein in InterPro IPR017248, HAX-1 |
PANTHERi | PTHR14938, PTHR14938, 1 hit |
PIRSFi | PIRSF037634, HS1-associating_X-1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAX1_MOUSE | |
Accessioni | O35387Primary (citable) accession number: O35387 Secondary accession number(s): Q542F8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families