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Protein

Serine/threonine-protein phosphatase with EF-hands 2

Gene

Ppef2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Activity regulationi

Activated by calcium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Manganese 1By similarity1
Metal bindingi181Manganese 1By similarity1
Metal bindingi208Manganese 1By similarity1
Metal bindingi208Manganese 2By similarity1
Metal bindingi240Manganese 2By similarity1
Active sitei241Proton donorBy similarity1
Metal bindingi292Manganese 2By similarity1
Metal bindingi492Manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi669 – 6801PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi709 – 7202PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processSensory transduction, Vision
LigandCalcium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase with EF-hands 2 (EC:3.1.3.16)
Short name:
PPEF-2
Gene namesi
Name:Ppef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1342304 Ppef2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589021 – 757Serine/threonine-protein phosphatase with EF-hands 2Add BLAST757

Proteomic databases

PaxDbiO35385
PRIDEiO35385

PTM databases

iPTMnetiO35385
PhosphoSitePlusiO35385

Expressioni

Tissue specificityi

Detected in retina, more specifically in photoreceptors.

Gene expression databases

BgeeiENSMUSG00000029410 Expressed in 39 organ(s), highest expression level in retina
GenevisibleiO35385 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031359

Structurei

3D structure databases

ProteinModelPortaliO35385
SMRiO35385
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 46IQPROSITE-ProRule annotationAdd BLAST26
Domaini572 – 607EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini656 – 691EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini696 – 731EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni128 – 544CatalyticAdd BLAST417

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0377 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000063173
HOGENOMiHOG000006820
HOVERGENiHBG008238
InParanoidiO35385
KOiK13807
OMAiLVDHFTP
OrthoDBiEOG091G02M5
PhylomeDBiO35385
TreeFamiTF313342

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di3.60.21.10, 2 hits
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000048 IQ_motif_EF-hand-BS
IPR029052 Metallo-depent_PP-like
IPR013235 PPP_dom
IPR012008 Ser/Thr-Pase_EF-hand_contain
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
PF00149 Metallophos, 1 hit
PF08321 PPP5, 1 hit
PIRSFiPIRSF000912 PPEF, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SM00156 PP2Ac, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
PS50096 IQ, 1 hit
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

O35385-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSSSSTQHH FAFQNAEKAF KAAALIQRWY RRYMARLEMR RRCTWNIFQS
60 70 80 90 100
IEYAGQQDQV KLHEFFSYLV DHFTPSSHHE RDFLNRMFTE ERFAQDVETE
110 120 130 140 150
EGGDFESIEV PDSYTGPRLS FPLLPDHATA LVEAFRLRQQ LHARYVLNLL
160 170 180 190 200
YETRKHLAQL PNINRVSTCY SEEVTVCGDL HGQLDDLIFI FYKNGLPSPE
210 220 230 240 250
RAYVFNGDFV DRGKDSVEVL MVLFAFMLVY PKEFHLNRGN HEDHLVNLRY
260 270 280 290 300
GFTKEVMHKY KIHGKKILRT LQDVFCWLPL ATLVDEKVLV LHGGVSDKTD
310 320 330 340 350
LELLAKLDRH KIVSTMRCKT RKESENREEQ KRKDNQTSSG QKPTPWFLPQ
360 370 380 390 400
SRSLPSSPFH LGSGFKAYKA GRSCSIPCGS PNSKELSRRG QVRRSVDLEL
410 420 430 440 450
EQCRQQAGFL GIREKGESLP LAPDADCVAD GGGVLEPTPE EWKQVVDILW
460 470 480 490 500
SDPAAQEGCK ANAVRGGGCY FGPDVTERLM EKYKLQLLIR SHECKPEGYE
510 520 530 540 550
FCHNRKVLTI FSASNYYEVG SNRGAYVKLG PALTPHIVQY QANKATHRLT
560 570 580 590 600
MRQRISRVEE SALRALRQKL FAHSSDLLVE FRKRDPDESG VITLSDWATA
610 620 630 640 650
VESVLHLGLP WRMLRPQLVN SSADNVLEYR SWLDSLAKEQ LSRENIQSSL
660 670 680 690 700
LEKLYRNRSN LETIFRIIDS DHSGFISLDE FRQTWKLFSS HMSIDITDDG
710 720 730 740 750
ICDLARSIDF NKDGHIDINE FLEAFRLVEQ SCLEGHASAC LQSTDTAESG

HSSPGPC
Length:757
Mass (Da):86,645
Last modified:January 1, 1998 - v1
Checksum:i821B3D1061AC00C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023458 mRNA Translation: AAB82798.1
BC027049 mRNA Translation: AAH27049.1
CCDSiCCDS39151.1
RefSeqiNP_035278.1, NM_011148.3
XP_006534880.1, XM_006534817.2
UniGeneiMm.87155

Genome annotation databases

EnsembliENSMUST00000031359; ENSMUSP00000031359; ENSMUSG00000029410
ENSMUST00000201130; ENSMUSP00000144157; ENSMUSG00000029410
GeneIDi19023
KEGGimmu:19023
UCSCiuc008yco.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPPE2_MOUSE
AccessioniPrimary (citable) accession number: O35385
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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