UniProtKB - O35350 (CAN1_MOUSE)
Protein
Calpain-1 catalytic subunit
Gene
Capn1
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity
Catalytic activityi
- Broad endopeptidase specificity.By similarity EC:3.4.22.52
Cofactori
Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity
Activity regulationi
Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 115 | By similarity | 1 | |
Active sitei | 272 | By similarity | 1 | |
Active sitei | 296 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 99 – 106 | 1PROSITE-ProRule annotation | 8 | |
Calcium bindingi | 302 – 333 | 2PROSITE-ProRule annotationAdd BLAST | 32 | |
Calcium bindingi | 597 – 608 | 3PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 627 – 638 | 4PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: MGI
- calcium ion binding Source: UniProtKB
- cytoskeletal protein binding Source: MGI
- enzyme binding Source: MGI
GO - Biological processi
- cellular response to hydrogen peroxide Source: MGI
- mammary gland involution Source: MGI
- negative regulation of actin filament polymerization Source: MGI
- negative regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of cardiac muscle cell apoptotic process Source: MGI
- positive regulation of leukocyte tethering or rolling Source: MGI
- positive regulation of vascular permeability Source: MGI
- protein autoprocessing Source: MGI
- proteolysis Source: MGI
- receptor catabolic process Source: MGI
- regulation of catalytic activity Source: UniProtKB
- response to angiotensin Source: MGI
- self proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-6798695, Neutrophil degranulation R-MMU-6809371, Formation of the cornified envelope |
Protein family/group databases
MEROPSi | C02.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)Alternative name(s): Calcium-activated neutral proteinase 1By similarity Short name: CANP 1By similarity Calpain mu-typeBy similarity Calpain-1 large subunitBy similarity Micromolar-calpain Short name: muCANPBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88263, Capn1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- Cytoplasm By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity
Cytosol
- cytosol Source: MGI
Lysosome
- lysosome Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207696 | 1 – 713 | Calpain-1 catalytic subunitAdd BLAST | 713 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 354 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 15 – 16 | Cleavage; for 78 kDa formBy similarity | 2 | |
Sitei | 27 – 28 | Cleavage; for 75 kDa formBy similarity | 2 |
Keywords - PTMi
Autocatalytic cleavage, PhosphoproteinProteomic databases
EPDi | O35350 |
jPOSTi | O35350 |
PaxDbi | O35350 |
PeptideAtlasi | O35350 |
PRIDEi | O35350 |
PTM databases
iPTMneti | O35350 |
PhosphoSitePlusi | O35350 |
SwissPalmi | O35350 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024942, Expressed in granulocyte and 172 other tissues |
ExpressionAtlasi | O35350, baseline and differential |
Genevisiblei | O35350, MM |
Interactioni
Subunit structurei
Forms a heterodimer with a small (regulatory) subunit CAPNS1.
By similarityGO - Molecular functioni
- cytoskeletal protein binding Source: MGI
- enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198470, 30 interactors |
ComplexPortali | CPX-4304, mu-Calpain complex |
DIPi | DIP-60303N |
IntActi | O35350, 5 interactors |
MINTi | O35350 |
STRINGi | 10090.ENSMUSP00000025891 |
Miscellaneous databases
RNActi | O35350, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 354 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 300 | |
Domaini | 540 – 575 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 584 – 617 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 614 – 649 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 679 – 713 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 355 – 525 | Domain IIIAdd BLAST | 171 | |
Regioni | 526 – 541 | LinkerAdd BLAST | 16 | |
Regioni | 542 – 712 | Domain IVAdd BLAST | 171 |
Sequence similaritiesi
Belongs to the peptidase C2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000159147 |
HOGENOMi | CLU_010982_0_1_1 |
InParanoidi | O35350 |
OMAi | GTPFEDQ |
OrthoDBi | 704215at2759 |
PhylomeDBi | O35350 |
TreeFami | TF314748 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O35350-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL
60 70 80 90 100
QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT
710
FDLFKWLQLT MFA
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A494BBB0 | A0A494BBB0_MOUSE | Calcium-activated neutral proteinas... | Capn1 | 699 | Annotation score: | ||
A0A494BAS0 | A0A494BAS0_MOUSE | Calpain-1 catalytic subunit | Capn1 | 196 | Annotation score: | ||
A0A494BB02 | A0A494BB02_MOUSE | Calpain-1 catalytic subunit | Capn1 | 79 | Annotation score: | ||
A0A494BAC4 | A0A494BAC4_MOUSE | Calpain-1 catalytic subunit | Capn1 | 79 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 488 | L → P in AAC33134 (Ref. 2) Curated | 1 | |
Sequence conflicti | 696 | D → N in AAC33134 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF021847 mRNA Translation: AAB72222.1 AF084459 mRNA Translation: AAC33134.1 BC026138 mRNA Translation: AAH26138.1 |
CCDSi | CCDS37893.1 |
RefSeqi | NP_001103974.1, NM_001110504.1 NP_031626.1, NM_007600.3 |
Genome annotation databases
Ensembli | ENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942 ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942 |
GeneIDi | 12333 |
KEGGi | mmu:12333 |
UCSCi | uc008ggj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF021847 mRNA Translation: AAB72222.1 AF084459 mRNA Translation: AAC33134.1 BC026138 mRNA Translation: AAH26138.1 |
CCDSi | CCDS37893.1 |
RefSeqi | NP_001103974.1, NM_001110504.1 NP_031626.1, NM_007600.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 198470, 30 interactors |
ComplexPortali | CPX-4304, mu-Calpain complex |
DIPi | DIP-60303N |
IntActi | O35350, 5 interactors |
MINTi | O35350 |
STRINGi | 10090.ENSMUSP00000025891 |
Protein family/group databases
MEROPSi | C02.001 |
PTM databases
iPTMneti | O35350 |
PhosphoSitePlusi | O35350 |
SwissPalmi | O35350 |
Proteomic databases
EPDi | O35350 |
jPOSTi | O35350 |
PaxDbi | O35350 |
PeptideAtlasi | O35350 |
PRIDEi | O35350 |
Protocols and materials databases
Antibodypediai | 1034, 548 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942 ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942 |
GeneIDi | 12333 |
KEGGi | mmu:12333 |
UCSCi | uc008ggj.2, mouse |
Organism-specific databases
CTDi | 823 |
MGIi | MGI:88263, Capn1 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000159147 |
HOGENOMi | CLU_010982_0_1_1 |
InParanoidi | O35350 |
OMAi | GTPFEDQ |
OrthoDBi | 704215at2759 |
PhylomeDBi | O35350 |
TreeFami | TF314748 |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-6798695, Neutrophil degranulation R-MMU-6809371, Formation of the cornified envelope |
Miscellaneous databases
BioGRID-ORCSi | 12333, 2 hits in 17 CRISPR screens |
ChiTaRSi | Capn1, mouse |
PROi | PR:O35350 |
RNActi | O35350, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024942, Expressed in granulocyte and 172 other tissues |
ExpressionAtlasi | O35350, baseline and differential |
Genevisiblei | O35350, MM |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN1_MOUSE | |
Accessioni | O35350Primary (citable) accession number: O35350 Secondary accession number(s): O88666 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries