Capn1

Functionⁱ

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityⁱ

Broad endopeptidase specificity.By similarity

Cofactorⁱ

Ca²⁺By similarityNote: Binds 4 Ca²⁺ ions.By similarity

Activity regulationⁱ

Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	115	By similarity	1
Active siteⁱ	272	By similarity	1
Active siteⁱ	296	By similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Calcium bindingⁱ	99 – 106	1PROSITE-ProRule annotation	8
Calcium bindingⁱ	302 – 333	2PROSITE-ProRule annotationAdd BLAST	32
Calcium bindingⁱ	597 – 608	3PROSITE-ProRule annotationAdd BLAST	12
Calcium bindingⁱ	627 – 638	4PROSITE-ProRule annotationAdd BLAST	12

GO - Molecular functionⁱ

calcium-dependent cysteine-type endopeptidase activity
Source: MGI
Inferred from direct assayⁱ
- 17529984
calcium ion binding Source: UniProtKB
cytoskeletal protein binding Source: MGI
enzyme binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mammary gland involution
Source: MGI
Inferred from mutant phenotypeⁱ
- 22555453
protein autoprocessing Source: MGI
proteolysis
Source: MGI
Inferred from mutant phenotypeⁱ
- 22555453
receptor catabolic process
Source: MGI
Inferred from direct assayⁱ
- 17529984
regulation of catalytic activity Source: UniProtKB
self proteolysis Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1474228 Degradation of the extracellular matrix R-MMU-6798695 Neutrophil degranulation R-MMU-6809371 Formation of the cornified envelope

Reactomeⁱ

R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-6798695 Neutrophil degranulation
R-MMU-6809371 Formation of the cornified envelope

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C02.001

MEROPSⁱ

C02.001

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity) Alternative name(s): Calcium-activated neutral proteinase 1By similarity Short name: CANP 1By similarity Calpain mu-typeBy similarity Calpain-1 large subunitBy similarity Micromolar-calpain Short name: muCANPBy similarity
Gene namesⁱ	Name:Capn1Imported Synonyms:Canp1, Capa1By similarity
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:88263 Capn1

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000207696	1 – 713	Calpain-1 catalytic subunitAdd BLAST		713

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	354	PhosphothreonineBy similarity		1

Post-translational modificationⁱ

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	15 – 16	Cleavage; for 78 kDa formBy similarity		2
Siteⁱ	27 – 28	Cleavage; for 75 kDa formBy similarity		2

Keywords - PTMⁱ

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

EPDⁱ	O35350
PaxDbⁱ	O35350
PeptideAtlas More...PeptideAtlasⁱ	O35350
PRIDEⁱ	O35350

PTM databases

iPTMnetⁱ	O35350
PhosphoSitePlusⁱ	O35350
SwissPalmⁱ	O35350

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000024942 Expressed in 147 organ(s), highest expression level in urinary bladder urothelium
CleanExⁱ	MM_CAPN1
ExpressionAtlasⁱ	O35350 baseline and differential
Genevisibleⁱ	O35350 MM

Interactionⁱ

Subunit structureⁱ

Forms a heterodimer with a small (regulatory) subunit CAPNS1.By similarity

GO - Molecular functionⁱ

cytoskeletal protein binding Source: MGI
enzyme binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	198470, 3 interactors
Database of interacting proteins More...DIPⁱ	DIP-60303N
IntActⁱ	O35350, 5 interactors
Molecular INTeraction database More...MINTⁱ	O35350
STRINGⁱ	10090.ENSMUSP00000025891

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O35350
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	55 – 354	Calpain catalyticPROSITE-ProRule annotationAdd BLAST	300
Domainⁱ	540 – 575	EF-hand 1PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	584 – 617	EF-hand 2PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	614 – 649	EF-hand 3PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	679 – 713	EF-hand 4PROSITE-ProRule annotationAdd BLAST	35

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	355 – 525	Domain IIIAdd BLAST	171
Regionⁱ	526 – 541	LinkerAdd BLAST	16
Regionⁱ	542 – 712	Domain IVAdd BLAST	171

Sequence similaritiesⁱ

Belongs to the peptidase C2 family.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0045 Eukaryota ENOG410XP0B LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118971
HOGENOMⁱ	HOG000232035
HOVERGENⁱ	HBG012645
InParanoidⁱ	O35350
KEGG Orthology (KO) More...KOⁱ	K01367
OMAⁱ	NFVTCLV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G049E
PhylomeDBⁱ	O35350
TreeFamⁱ	TF314748

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00214 Calpain_III, 1 hit cd00044 CysPc, 1 hit
InterProⁱ	View protein in InterPro IPR033883 C2_III IPR022684 Calpain_cysteine_protease IPR022682 Calpain_domain_III IPR022683 Calpain_III IPR036213 Calpain_III_sf IPR029643 CAPN1 IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR038765 Papain_like_cys_pep_sf IPR000169 Pept_cys_AS IPR001300 Peptidase_C2_calpain_cat
PANTHERⁱ	PTHR10183:SF284 PTHR10183:SF284, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01067 Calpain_III, 1 hit PF13833 EF-hand_8, 1 hit PF00648 Peptidase_C2, 1 hit
PRINTSⁱ	PR00704 CALPAIN
SMARTⁱ	View protein in SMART SM00720 calpain_III, 1 hit SM00230 CysPc, 1 hit
SUPFAMⁱ	SSF47473 SSF47473, 1 hit SSF49758 SSF49758, 1 hit SSF54001 SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50203 CALPAIN_CAT, 1 hit PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O35350-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL 
        60         70         80         90        100
QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD 
       110        120        130        140        150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQDGYAGIFH 
       160        170        180        190        200
FQLWQFGEWV DVVIDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG 
       210        220        230        240        250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC 
       260        270        280        290        300
SINISDIRDL EAITFKNLVR GHAYSVTGAK QVTYQGQRVN LIRMRNPWGE 
       310        320        330        340        350
VEWKGPWSDS SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI 
       360        370        380        390        400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR 
       410        420        430        440        450
LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR 
       460        470        480        490        500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF 
       510        520        530        540        550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF 
       560        570        580        590        600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG 
       610        620        630        640        650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL 
       660        670        680        690        700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTDLDGVVT 
       710 
FDLFKWLQLT MFA

Length:713

Mass (Da):82,106

Last modified:January 1, 1998 - v1

Checksum:ⁱ3E1E26C95802B864

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	488	L → P in AAC33134 (Ref. 2) Curated		1
Sequence conflictⁱ	696	D → N in AAC33134 (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF021847 mRNA Translation: AAB72222.1 AF084459 mRNA Translation: AAC33134.1 BC026138 mRNA Translation: AAH26138.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37893.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001103974.1, NM_001110504.1 NP_031626.1, NM_007600.3
UniGeneⁱ	Mm.6221

Genome annotation databases

Ensemblⁱ	ENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942 ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942
GeneIDⁱ	12333
KEGGⁱ	mmu:12333
UCSC genome browser More...UCSCⁱ	uc008ggj.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O35350	Uncharacterized protein		MOUSE		713	UniRef100_O35350

Protein	Similar proteins		Species	Score	Length	Source
O35350	Calpain-1 catalytic subunit	)	RAT		713	UniRef90_O35350
Uncharacterized protein		MOUSE		713
calpain-1 catalytic subunit		MESAU		122
UPI00042AAE9B		PERMB		713
UPI000A307453		MUSPA		713
+8

Protein	Similar proteins		Species	Score	Length	Source
O35350	Calpain-1 catalytic subunit	)	HUMAN		714	UniRef50_P07384
Calpain-1 catalytic subunit		BOVIN		716
Calpain-1 catalytic subunit		PONAB		714
Calpain-1 catalytic subunit		PIG		714
Calpain-1 catalytic subunit		CHICK		705
+897

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF021847 mRNA Translation: AAB72222.1 AF084459 mRNA Translation: AAC33134.1 BC026138 mRNA Translation: AAH26138.1
CCDSⁱ	CCDS37893.1
RefSeqⁱ	NP_001103974.1, NM_001110504.1 NP_031626.1, NM_007600.3
UniGeneⁱ	Mm.6221

3D structure databases

ProteinModelPortalⁱ	O35350
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	198470, 3 interactors
DIPⁱ	DIP-60303N
IntActⁱ	O35350, 5 interactors
MINTⁱ	O35350
STRINGⁱ	10090.ENSMUSP00000025891

Protein family/group databases

MEROPSⁱ	C02.001

MEROPSⁱ

C02.001

PTM databases

iPTMnetⁱ	O35350
PhosphoSitePlusⁱ	O35350
SwissPalmⁱ	O35350

Proteomic databases

EPDⁱ	O35350
PaxDbⁱ	O35350
PeptideAtlasⁱ	O35350
PRIDEⁱ	O35350

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025891; ENSMUSP00000025891; ENSMUSG00000024942 ENSMUST00000164843; ENSMUSP00000127498; ENSMUSG00000024942
GeneIDⁱ	12333
KEGGⁱ	mmu:12333
UCSCⁱ	uc008ggj.2 mouse

Organism-specific databases

CTDⁱ	823
MGIⁱ	MGI:88263 Capn1

Phylogenomic databases

eggNOGⁱ	KOG0045 Eukaryota ENOG410XP0B LUCA
GeneTreeⁱ	ENSGT00760000118971
HOGENOMⁱ	HOG000232035
HOVERGENⁱ	HBG012645
InParanoidⁱ	O35350
KOⁱ	K01367
OMAⁱ	NFVTCLV
OrthoDBⁱ	EOG091G049E
PhylomeDBⁱ	O35350
TreeFamⁱ	TF314748

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1474228 Degradation of the extracellular matrix R-MMU-6798695 Neutrophil degranulation R-MMU-6809371 Formation of the cornified envelope

Reactomeⁱ

R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-6798695 Neutrophil degranulation
R-MMU-6809371 Formation of the cornified envelope

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O35350
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024942 Expressed in 147 organ(s), highest expression level in urinary bladder urothelium
CleanExⁱ	MM_CAPN1
ExpressionAtlasⁱ	O35350 baseline and differential
Genevisibleⁱ	O35350 MM

Family and domain databases

CDDⁱ	cd00214 Calpain_III, 1 hit cd00044 CysPc, 1 hit
InterProⁱ	View protein in InterPro IPR033883 C2_III IPR022684 Calpain_cysteine_protease IPR022682 Calpain_domain_III IPR022683 Calpain_III IPR036213 Calpain_III_sf IPR029643 CAPN1 IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR038765 Papain_like_cys_pep_sf IPR000169 Pept_cys_AS IPR001300 Peptidase_C2_calpain_cat
PANTHERⁱ	PTHR10183:SF284 PTHR10183:SF284, 1 hit
Pfamⁱ	View protein in Pfam PF01067 Calpain_III, 1 hit PF13833 EF-hand_8, 1 hit PF00648 Peptidase_C2, 1 hit
PRINTSⁱ	PR00704 CALPAIN
SMARTⁱ	View protein in SMART SM00720 calpain_III, 1 hit SM00230 CysPc, 1 hit
SUPFAMⁱ	SSF47473 SSF47473, 1 hit SSF49758 SSF49758, 1 hit SSF54001 SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50203 CALPAIN_CAT, 1 hit PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CAN1_MOUSE
Accessionⁱ	O35350Primary (citable) accession number: O35350 Secondary accession number(s): O88666
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
	Last sequence update:	January 1, 1998
	Last modified:	November 7, 2018
	This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Peptidase families
Classification of peptidase families and list of entries
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O35350 (CAN1_MOUSE)

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Calpain-1 catalytic subunit

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Cytosol

Lysosome

Mitochondrion

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ