Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 1

Gene

Hs3st1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei151PAPS1 Publication1
Binding sitei159PAPS1 Publication1
Binding sitei259PAPS1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 72PAPS1 Publication5
Nucleotide bindingi274 – 278PAPS1 Publication5

GO - Molecular functioni

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.8.2.23 3474
ReactomeiR-MMU-2022928 HS-GAG biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 1 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
Short name:
Heparan sulfate 3-O-sulfotransferase 1
Gene namesi
Name:Hs3st1
Synonyms:3ost, 3ost1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1201606 Hs3st1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67R → E or A: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi68K → A: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi72R → A or E: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi76E → A or Q: Strongly decreases the enzymatic activity. 1 Publication1
Mutagenesisi88E → A: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi89N → A or D: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi90E → Q or A: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi92H → F or A: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi95D → A or N: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi96W → A or F: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi98E → A: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi123K → A: Abolishes the enzymatic activity. 1 Publication1
Mutagenesisi132K → A: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi163Q → A: Strongly decreases the enzymatic activity. 1 Publication1
Mutagenesisi197R → A: Weakly decrease the enzymatic activity. 1 Publication1
Mutagenesisi271H → A: No effect. 1 Publication1
Mutagenesisi271H → F: Decreases the enzymatic activity. 1 Publication1
Mutagenesisi273S → A: Weakly decrease the enzymatic activity. 1 Publication1
Mutagenesisi274K → A: Strongly decrease the enzymatic activity. 1 Publication1
Mutagenesisi276R → A: Abolishes the enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000003345221 – 311Heparan sulfate glucosamine 3-O-sulfotransferase 1Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi246N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi260 ↔ 2691 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO35310
PRIDEiO35310

PTM databases

PhosphoSitePlusiO35310

Expressioni

Gene expression databases

BgeeiENSMUSG00000051022 Expressed in 227 organ(s), highest expression level in cumulus cell
ExpressionAtlasiO35310 baseline and differential
GenevisibleiO35310 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051055

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO35310
SMRiO35310
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35310

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiO35310
KOiK01024
OMAiGFYCLRD
OrthoDBiEOG091G0CS5
PhylomeDBiO35310
TreeFamiTF350755

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O35310-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLLLLGAVL LVAQPQLVHS HPAAPGPGLK QQELLRKVII LPEDTGEGTA
60 70 80 90 100
SNGSTQQLPQ TIIIGVRKGG TRALLEMLSL HPDVAAAENE VHFFDWEEHY
110 120 130 140 150
SQGLGWYLTQ MPFSSPHQLT VEKTPAYFTS PKVPERIHSM NPTIRLLLIL
160 170 180 190 200
RDPSERVLSD YTQVLYNHLQ KHKPYPPIED LLMRDGRLNL DYKALNRSLY
210 220 230 240 250
HAHMLNWLRF FPLGHIHIVD GDRLIRDPFP EIQKVERFLK LSPQINASNF
260 270 280 290 300
YFNKTKGFYC LRDSGKDRCL HESKGRAHPQ VDPKLLDKLH EYFHEPNKKF
310
FKLVGRTFDW H
Length:311
Mass (Da):35,899
Last modified:January 1, 1998 - v1
Checksum:i7DCFAC494C838FD7
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YUR4D3YUR4_MOUSE
Sulfotransferase
Hs3st1
176Annotation score:
D3YZM3D3YZM3_MOUSE
Heparan sulfate glucosamine 3-O-sul...
Hs3st1
75Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019385 mRNA Translation: AAB84387.1
AK087753 mRNA Translation: BAC39991.1
BC009133 mRNA Translation: AAH09133.1
CCDSiCCDS19257.1
RefSeqiNP_034604.1, NM_010474.2
XP_017176175.1, XM_017320686.1
UniGeneiMm.12559

Genome annotation databases

EnsembliENSMUST00000053116; ENSMUSP00000051055; ENSMUSG00000051022
ENSMUST00000117944; ENSMUSP00000113919; ENSMUSG00000051022
GeneIDi15476
KEGGimmu:15476
UCSCiuc008xgy.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019385 mRNA Translation: AAB84387.1
AK087753 mRNA Translation: BAC39991.1
BC009133 mRNA Translation: AAH09133.1
CCDSiCCDS19257.1
RefSeqiNP_034604.1, NM_010474.2
XP_017176175.1, XM_017320686.1
UniGeneiMm.12559

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VKJX-ray2.50A/B/C48-311[»]
3UANX-ray1.84A/B48-311[»]
ProteinModelPortaliO35310
SMRiO35310
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051055

PTM databases

PhosphoSitePlusiO35310

Proteomic databases

PaxDbiO35310
PRIDEiO35310

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053116; ENSMUSP00000051055; ENSMUSG00000051022
ENSMUST00000117944; ENSMUSP00000113919; ENSMUSG00000051022
GeneIDi15476
KEGGimmu:15476
UCSCiuc008xgy.2 mouse

Organism-specific databases

CTDi9957
MGIiMGI:1201606 Hs3st1

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiO35310
KOiK01024
OMAiGFYCLRD
OrthoDBiEOG091G0CS5
PhylomeDBiO35310
TreeFamiTF350755

Enzyme and pathway databases

BRENDAi2.8.2.23 3474
ReactomeiR-MMU-2022928 HS-GAG biosynthesis

Miscellaneous databases

EvolutionaryTraceiO35310
PROiPR:O35310
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051022 Expressed in 227 organ(s), highest expression level in cumulus cell
ExpressionAtlasiO35310 baseline and differential
GenevisibleiO35310 MM

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS3S1_MOUSE
AccessioniPrimary (citable) accession number: O35310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again