Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vesicular acetylcholine transporter

Gene

Slc18a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.By similarity

GO - Molecular functioni

GO - Biological processi

  • acetylcholine transport Source: MGI
  • neurotransmitter loading into synaptic vesicle Source: SynGO

Keywordsi

Biological processNeurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:Slc18a3
Synonyms:Vacht
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1101061 Slc18a3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 125Lumenal, vesicleSequence analysisAdd BLAST71
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 152CytoplasmicSequence analysis6
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 182Lumenal, vesicleSequence analysis9
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 213CytoplasmicSequence analysis10
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 242Lumenal, vesicleSequence analysis8
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 288CytoplasmicSequence analysisAdd BLAST25
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21
Topological domaini310 – 325Lumenal, vesicleSequence analysisAdd BLAST16
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 356CytoplasmicSequence analysis10
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 388Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 422CytoplasmicSequence analysisAdd BLAST13
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 447Lumenal, vesicleSequence analysis4
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 530CytoplasmicSequence analysisAdd BLAST62

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275191 – 530Vesicular acetylcholine transporterAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO35304

PTM databases

iPTMnetiO35304
PhosphoSitePlusiO35304

Expressioni

Tissue specificityi

Expressed in the spinal cord, brain (excluding the cerebellum), brain stem and cholinergic tissues. Not expressed in peripheral tissues such as liver and kidney.1 Publication

Interactioni

Subunit structurei

Interacts with SEC14L1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO35304
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni471 – 530Mediates interaction with SEC14L11 PublicationAdd BLAST60

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG055082
InParanoidiO35304

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35304-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGSES PTLISEVWEP TLPPPTLANA SAYLANTSAS
110 120 130 140 150
PTAAGSARSI LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVMFA FAEDYATFFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
Length:530
Mass (Da):56,615
Last modified:January 1, 1998 - v1
Checksum:i08526F29B4E8EEDD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TYJ1Q3TYJ1_MOUSE
Solute carrier family 18 (Vesicular...
Slc18a3 mCG_6362
530Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019045 Genomic DNA Translation: AAD09151.1
CCDSiCCDS79291.1
UniGeneiMm.190503

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019045 Genomic DNA Translation: AAD09151.1
CCDSiCCDS79291.1
UniGeneiMm.190503

3D structure databases

ProteinModelPortaliO35304
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO35304
PhosphoSitePlusiO35304

Proteomic databases

PRIDEiO35304

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1101061 Slc18a3

Phylogenomic databases

HOVERGENiHBG055082
InParanoidiO35304

Miscellaneous databases

PROiPR:O35304
SOURCEiSearch...

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVACHT_MOUSE
AccessioniPrimary (citable) accession number: O35304
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again