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Protein

Acyloxyacyl hydrolase

Gene

Aoah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS) (PubMed:12810692, PubMed:15155618, PubMed:17322564, PubMed:19860560). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (PubMed:17322564, PubMed:19860560, PubMed:28622363). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (PubMed:18779055).6 Publications

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.5 Publications

Cofactori

Ca2+1 PublicationNote: Binds 3 Ca2+ ions per subunit. The calcium ions probably have a structural role.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi183Calcium 1Combined sources1 Publication1
Metal bindingi185Calcium 1Combined sources1 Publication1
Metal bindingi185Calcium 2Combined sources1 Publication1
Metal bindingi187Calcium 1Combined sources1 Publication1
Metal bindingi187Calcium 2Combined sources1 Publication1
Metal bindingi189Calcium 1; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi204Calcium 1Combined sources1 Publication1
Metal bindingi204Calcium 2Combined sources1 Publication1
Metal bindingi206Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi207Calcium 1Combined sources1 Publication1
Metal bindingi209Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi212Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi222Calcium 3Combined sources1 Publication1
Metal bindingi226Calcium 3Combined sources1 Publication1
Metal bindingi228Calcium 3Combined sources1 Publication1
Metal bindingi230Calcium 3Combined sources1 Publication1
Metal bindingi232Calcium 3; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi244Calcium 3Combined sources1 Publication1
Active sitei2621 Publication1
Binding sitei344LipopolysaccharideBy similarity1

GO - Molecular functioni

  • acyloxyacyl hydrolase activity Source: MGI
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.77 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Acyloxyacyl hydrolase1 Publication (EC:3.1.1.775 Publications)
Cleaved into the following 2 chains:
Acyloxyacyl hydrolase small subunitBy similarity
Acyloxyacyl hydrolase large subunitBy similarity
Gene namesi
Name:Aoah1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1350928 Aoah

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Secreted

Pathology & Biotechi

Disruption phenotypei

Animals develop significant and prolonged hepatosplenomegaly in response to bacterial lipopolysaccharide (LPS) challenge (PubMed:17322564). Both liver and spleen show increased accumulation of leukocytes (PubMed:17322564). Significantly reduced deacylation of LPS in liver and spleen, in peritoneal macrophages, and in bone marrow-derived dendritic cells (PubMed:12810692, PubMed:17322564, PubMed:18779055). Increased lung injury, delayed neutrophil clearance, and prolonged recovery time in response to intranasal LPS administration (PubMed:28622363). Alveolar macrophages show persistent activation and cytokine levels in lung remain elevated 4-7 days after LPS administration (PubMed:28622363). Impaired response to a second infection challenge, with reduced production of the pro-inflammatory chemokines CCL5/RANTES, TNF and IL6 by peritoneal macrophages and reduced survival rates, indicating a prolonged state of immune tolerance (PubMed:18779055). This immune tolerant state can perisist for two months or longer (PubMed:18779055).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi262S → A: Loss of catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
PropeptideiPRO_000004181223 – 33By similarityAdd BLAST11
ChainiPRO_000004181334 – 155Acyloxyacyl hydrolase small subunitBy similarityAdd BLAST122
ChainiPRO_0000041814156 – 574Acyloxyacyl hydrolase large subunitBy similarityAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 113PROSITE-ProRule annotationCombined sources1 Publication
Disulfide bondi43 ↔ 107PROSITE-ProRule annotationCombined sources1 Publication
Glycosylationi58N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi69 ↔ 82PROSITE-ProRule annotationCombined sources1 Publication
Disulfide bondi122 ↔ 452Combined sources1 Publication
Disulfide bondi122 ↔ 452Interchain (between small and large subunit)By similarity
Disulfide bondi159 ↔ 168Combined sources1 Publication
Disulfide bondi205 ↔ 229Combined sources1 Publication
Glycosylationi206N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Disulfide bondi248 ↔ 328Combined sources1 Publication
Disulfide bondi375 ↔ 458Combined sources1 Publication
Glycosylationi408N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi465N-linked (GlcNAc...) asparagineCombined sources1 Publication1

Post-translational modificationi

Cleaved into a large and a small subunit.By similarity
The small subunit is N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiO35298
PaxDbiO35298
PRIDEiO35298

PTM databases

iPTMnetiO35298
PhosphoSitePlusiO35298

Expressioni

Tissue specificityi

Detected in peritoneal macrophages (at protein level) (PubMed:17322564, PubMed:28622363). Strongly expressed in kidney cortex, where it may be produced by proximal tubule cells (PubMed:15155618). In liver, expressed at high levels in Kupffer cells (PubMed:17322564). Expressed by dendritic cells (PubMed:12810692). Detected at low levels in alveolar macrophages (PubMed:28622363).4 Publications

Inductioni

Strongly up-regulated in alveolar macrophages in response to bacterial lipopolysaccharides (LPS).1 Publication

Gene expression databases

BgeeiENSMUSG00000021322 Expressed in 57 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_AOAH
ExpressionAtlasiO35298 baseline and differential
GenevisibleiO35298 MM

Interactioni

Subunit structurei

Heterodimer of the large and small subunits; disulfide-linked.2 Publications

Structurei

Secondary structure

1574
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO35298
SMRiO35298
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 117Saposin B-typePROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 69Important for enzyme activity, localization to cytoplasmic vesicles, and protein stabilityBy similarityAdd BLAST33
Regioni172 – 176Lipopolysaccharide bindingBy similarity5

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFCC Eukaryota
ENOG410XTCS LUCA
GeneTreeiENSGT00390000008427
HOGENOMiHOG000008100
HOVERGENiHBG004254
InParanoidiO35298
KOiK01065
OMAiDPKDGIP
OrthoDBiEOG091G03MF
PhylomeDBiO35298
TreeFamiTF329246

Family and domain databases

InterProiView protein in InterPro
IPR039676 AOAH
IPR001087 GDSL
IPR011001 Saposin-like
IPR008139 SaposinB_dom
PANTHERiPTHR15010 PTHR15010, 1 hit
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 1 hit
SMARTiView protein in SMART
SM00741 SapB, 1 hit
SUPFAMiSSF47862 SSF47862, 1 hit
PROSITEiView protein in PROSITE
PS50015 SAP_B, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35298-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFPWKVFKT TLLLLLLSHS LASVPSEDQP GDSYSHGQSC LGCVVLVSVI
60 70 80 90 100
EQLAEVHNSS VQVAMERLCS YLPEKLFLKT ACYFLVQTFG SDIIKLLDEA
110 120 130 140 150
MKADVVCYAL EFCKRGAVQP QCHLYPLPQE AWESALEKAR QVLRRSSTMK
160 170 180 190 200
YPRSGRNICS LPFLTKICQK IELSIKKAVP FKDIDSDKHS VFPTLRGYHW
210 220 230 240 250
RGRDCNDSDK TVYPGRRPDN WDIHQDSNCN GIWGIDPKDG IPYEKKFCEG
260 270 280 290 300
SQPRGIILLG DSAGAHFHIP PEWLTASQMS VNSFLNLPSA LTDELNWPQL
310 320 330 340 350
SGVTGFLDST SGIEEKSIYH RLRKRNHCNH RDYQSISKNG ASSRNLKNFI
360 370 380 390 400
ESLSRNQASD HPAIVLYAMI GNDVCNSKAD TVPEMTTPEQ MYANVMQTLT
410 420 430 440 450
HLNSHLPNGS HVILYGLPDG TFLWDSLHNR YHPLGQLNKD VTYAQFFSFL
460 470 480 490 500
RCLQLNPCNG WMSSNKTLRT LTSERAEQLS NTLKKIATTE TFANFDLFYV
510 520 530 540 550
DFAFHEIIED WQKRGGQPWQ LIEPVDGFHP NEVASLLQAN RVWEKIQLQW
560 570
PHVLGKENPF NSQIEEVFGD QGGH
Length:574
Mass (Da):65,155
Last modified:January 1, 1998 - v1
Checksum:i10B32C3BE772FB68
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5FW74Q5FW74_MOUSE
Acyloxyacyl hydrolase
Aoah
145Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018172 mRNA Translation: AAB81182.1
AK084452 mRNA Translation: BAC39187.1
CCDSiCCDS26266.1
RefSeqiNP_001268783.1, NM_001281854.1
NP_036184.1, NM_012054.4
UniGeneiMm.314046

Genome annotation databases

EnsembliENSMUST00000021757; ENSMUSP00000021757; ENSMUSG00000021322
GeneIDi27052
KEGGimmu:27052
UCSCiuc007ppu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018172 mRNA Translation: AAB81182.1
AK084452 mRNA Translation: BAC39187.1
CCDSiCCDS26266.1
RefSeqiNP_001268783.1, NM_001281854.1
NP_036184.1, NM_012054.4
UniGeneiMm.314046

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W7DX-ray1.75A23-574[»]
5W7EX-ray1.83A/B23-574[»]
5W7FX-ray2.80A/B23-574[»]
ProteinModelPortaliO35298
SMRiO35298
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO35298
PhosphoSitePlusiO35298

Proteomic databases

MaxQBiO35298
PaxDbiO35298
PRIDEiO35298

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021757; ENSMUSP00000021757; ENSMUSG00000021322
GeneIDi27052
KEGGimmu:27052
UCSCiuc007ppu.2 mouse

Organism-specific databases

CTDi313
MGIiMGI:1350928 Aoah

Phylogenomic databases

eggNOGiENOG410IFCC Eukaryota
ENOG410XTCS LUCA
GeneTreeiENSGT00390000008427
HOGENOMiHOG000008100
HOVERGENiHBG004254
InParanoidiO35298
KOiK01065
OMAiDPKDGIP
OrthoDBiEOG091G03MF
PhylomeDBiO35298
TreeFamiTF329246

Enzyme and pathway databases

BRENDAi3.1.1.77 3474

Miscellaneous databases

ChiTaRSiAoah mouse
PROiPR:O35298
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021322 Expressed in 57 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_AOAH
ExpressionAtlasiO35298 baseline and differential
GenevisibleiO35298 MM

Family and domain databases

InterProiView protein in InterPro
IPR039676 AOAH
IPR001087 GDSL
IPR011001 Saposin-like
IPR008139 SaposinB_dom
PANTHERiPTHR15010 PTHR15010, 1 hit
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 1 hit
SMARTiView protein in SMART
SM00741 SapB, 1 hit
SUPFAMiSSF47862 SSF47862, 1 hit
PROSITEiView protein in PROSITE
PS50015 SAP_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAOAH_MOUSE
AccessioniPrimary (citable) accession number: O35298
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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