Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease 2B

Gene

Rnase2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38Proton acceptorBy similarity1
Active sitei150Proton donorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6803157 Antimicrobial peptides

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 2BImported (EC:3.1.27.5By similarity)
Alternative name(s):
Eosinophil cationic-type ribonuclease 5Curated
MR-5Curated
Gene namesi
Name:Rnase2bImported
Synonyms:Ear5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1858598 Rnase2b

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003087026 – 155Ribonuclease 2BCuratedAdd BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 106By similarity
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Disulfide bondi86 ↔ 94By similarity
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PaxDbiO35292
PRIDEiO35292

PTM databases

iPTMnetiO35292
PhosphoSitePlusiO35292

Expressioni

Gene expression databases

BgeeiENSMUSG00000059606 Expressed in 35 organ(s), highest expression level in ear
ExpressionAtlasiO35292 baseline and differential
GenevisibleiO35292 MM

Structurei

3D structure databases

ProteinModelPortaliO35292
SMRiO35292
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 66Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JDT3 Eukaryota
ENOG411136R LUCA
GeneTreeiENSGT00900000140993
HOGENOMiHOG000276882
HOVERGENiHBG008396
InParanoidiO35292
OMAiNQSIRCP
OrthoDBiEOG091G0SFK
TreeFamiTF333393

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35292-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLKLLESRL CLLLLLGLVL TLVSCQRPTP SQKFDIQHIY KKSSPKCDDA
60 70 80 90 100
MRVVNKYTGK CKDLNTFLHT TFADVVRVCH NPPKTCKDGT SPNCHDSSSK
110 120 130 140 150
VSVTICKLTK RARNYTHCRY KTTGAKKSYT VACNPRTPKD RPTYPVVPVH

LDRLF
Length:155
Mass (Da):17,514
Last modified:October 3, 2012 - v2
Checksum:iE22D48C15634065A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35D → A in AAC53491 (PubMed:9336452).Curated1
Sequence conflicti125A → G in AAC53491 (PubMed:9336452).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA Translation: AAC53491.1
AF512013 Genomic DNA Translation: AAM44856.1
AC163664 Genomic DNA No translation available.
CH466605 Genomic DNA Translation: EDL20848.1
BC117776 mRNA Translation: AAI17777.1
BC119506 mRNA Translation: AAI19507.1
CCDSiCCDS27039.1
RefSeqiNP_062271.2, NM_019398.2
UniGeneiMm.377125

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606
GeneIDi54159
KEGGimmu:54159
UCSCiuc007tmt.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA Translation: AAC53491.1
AF512013 Genomic DNA Translation: AAM44856.1
AC163664 Genomic DNA No translation available.
CH466605 Genomic DNA Translation: EDL20848.1
BC117776 mRNA Translation: AAI17777.1
BC119506 mRNA Translation: AAI19507.1
CCDSiCCDS27039.1
RefSeqiNP_062271.2, NM_019398.2
UniGeneiMm.377125

3D structure databases

ProteinModelPortaliO35292
SMRiO35292
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO35292
PhosphoSitePlusiO35292

Proteomic databases

PaxDbiO35292
PRIDEiO35292

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606
GeneIDi54159
KEGGimmu:54159
UCSCiuc007tmt.2 mouse

Organism-specific databases

CTDi54159
MGIiMGI:1858598 Rnase2b

Phylogenomic databases

eggNOGiENOG410JDT3 Eukaryota
ENOG411136R LUCA
GeneTreeiENSGT00900000140993
HOGENOMiHOG000276882
HOVERGENiHBG008396
InParanoidiO35292
OMAiNQSIRCP
OrthoDBiEOG091G0SFK
TreeFamiTF333393

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6803157 Antimicrobial peptides

Miscellaneous databases

PROiPR:O35292
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059606 Expressed in 35 organ(s), highest expression level in ear
ExpressionAtlasiO35292 baseline and differential
GenevisibleiO35292 MM

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNS2B_MOUSE
AccessioniPrimary (citable) accession number: O35292
Secondary accession number(s): Q8K416
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2012
Last modified: September 12, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again