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Protein

Non-secretory ribonuclease

Gene

Rnase2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33SubstrateBy similarity1
Active sitei38Proton acceptorBy similarity1
Active sitei150Proton donorBy similarity1

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • ribonuclease A activity Source: UniProtKB-EC
  • ribonuclease activity Source: GO_Central

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Non-secretory ribonuclease (EC:3.1.27.5)
Alternative name(s):
Eosinophil cationic-type ribonuclease 4
MR-4
Ribonuclease 2
Short name:
RNase 2
Gene namesi
Name:Rnase2
Synonyms:Ear4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1858238 Ear4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003088226 – 155Non-secretory ribonucleaseAdd BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi47 ↔ 106By similarity
Modified residuei57Nitrated tyrosineBy similarity1
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi86 ↔ 94By similarity
Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PRIDEiO35291

PTM databases

PhosphoSitePlusiO35291

Interactioni

Subunit structurei

Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO35291
SMRiO35291
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 66Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000276882
HOVERGENiHBG008396
InParanoidiO35291
KOiK01168
PhylomeDBiO35291

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35291-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPKLLESRL CLLLLLGLVL MLASCQAQIL SQKFYTQHIY NSTYPRCDAV
60 70 80 90 100
MRVVNRYRPR CKDINTFLHT SFADVVAVCG HPNITCNNLT RKNCHASSFQ
110 120 130 140 150
VFITFCNLTM PTRICTQCRY QTTGSVKYYR VACENRTPQD TPMYPVVPVH

LDGTF
Length:155
Mass (Da):17,723
Last modified:January 1, 1998 - v1
Checksum:i36879AAF1CCFB961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017259 Genomic DNA Translation: AAC53490.1
RefSeqiNP_001017422.1, NM_001017422.1
UniGeneiMm.377124

Genome annotation databases

GeneIDi53877
KEGGimmu:53877

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017259 Genomic DNA Translation: AAC53490.1
RefSeqiNP_001017422.1, NM_001017422.1
UniGeneiMm.377124

3D structure databases

ProteinModelPortaliO35291
SMRiO35291
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSitePlusiO35291

Proteomic databases

PRIDEiO35291

Protocols and materials databases

DNASUi53877
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi53877
KEGGimmu:53877

Organism-specific databases

CTDi53877
MGIiMGI:1858238 Ear4

Phylogenomic databases

HOGENOMiHOG000276882
HOVERGENiHBG008396
InParanoidiO35291
KOiK01168
PhylomeDBiO35291

Miscellaneous databases

PROiPR:O35291
SOURCEiSearch...

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNAS2_MOUSE
AccessioniPrimary (citable) accession number: O35291
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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