UniProtKB - O35286 (DHX15_MOUSE)
Protein
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Gene
Dhx15
Organism
Mus musculus (Mouse)
Status
Functioni
Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (By similarity).By similarity
Catalytic activityi
- EC:3.6.4.13By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 167 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- double-stranded RNA binding Source: MGI
- RNA binding Source: GO_Central
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- mRNA processing Source: UniProtKB-KW
- response to alkaloid Source: Ensembl
- response to toxic substance Source: Ensembl
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | Helicase, Hydrolase |
Biological process | mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15By similarity (EC:3.6.4.13By similarity)Alternative name(s): DEAH box protein 15 |
Gene namesi | Name:Dhx15 Synonyms:Ddx15, Deah9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1099786, Dhx15 |
Subcellular locationi
Nucleus
- nuclear speck Source: MGI
- nucleolus Source: UniProtKB-SubCell
- spliceosomal complex Source: GO_Central
- U12-type spliceosomal complex Source: HGNC-UCL
- U2-type post-mRNA release spliceosomal complex Source: Ensembl
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055140 | 1 – 795 | Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15Add BLAST | 795 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineBy similarity | 1 | |
Modified residuei | 488 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 786 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O35286 |
jPOSTi | O35286 |
PaxDbi | O35286 |
PeptideAtlasi | O35286 |
PRIDEi | O35286 |
PTM databases
iPTMneti | O35286 |
PhosphoSitePlusi | O35286 |
SwissPalmi | O35286 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029169, Expressed in primitive streak and 287 other tissues |
Genevisiblei | O35286, MM |
Interactioni
Subunit structurei
Interacts with SSB/La.
Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient.
Interacts with TFIP11; indicative for a recruitment to the IL complex.
Interacts with GPATCH2.
By similarityBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 199083, 54 interactors |
CORUMi | O35286 |
DIPi | DIP-61875N |
IntActi | O35286, 7 interactors |
MINTi | O35286 |
STRINGi | 10090.ENSMUSP00000031061 |
Miscellaneous databases
RNActi | O35286, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 147 – 313 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 338 – 518 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 181 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 260 – 263 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 346 – 349 | Poly-Glu | 4 | |
Compositional biasi | 395 – 398 | Poly-Gln | 4 | |
Compositional biasi | 403 – 406 | Poly-Pro | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
GeneTreei | ENSGT00940000155800 |
InParanoidi | O35286 |
OMAi | QWCVDFA |
OrthoDBi | 354219at2759 |
PhylomeDBi | O35286 |
TreeFami | TF105735 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O35286-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKRHRLDLG EDYPSGKKRA GTDGKDRERD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS
110 120 130 140 150
THTGHTGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ497W9 | Q497W9_MOUSE | RNA helicase | Dhx15 | 703 | Annotation score: | ||
A0A0G2JG10 | A0A0G2JG10_MOUSE | RNA helicase | Dhx15 | 608 | Annotation score: | ||
A0A0G2JGQ5 | A0A0G2JGQ5_MOUSE | Pre-mRNA-splicing factor ATP-depend... | Dhx15 | 186 | Annotation score: |
Sequence cautioni
The sequence BC003745 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 388 – 389 | LY → FS (PubMed:9342318).Curated | 2 | |
Sequence conflicti | 391 – 393 | TLP → YTS (PubMed:9342318).Curated | 3 | |
Sequence conflicti | 757 – 758 | WL → CW (PubMed:9342318).Curated | 2 | |
Sequence conflicti | 759 – 795 | Missing (PubMed:9342318).CuratedAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017153 mRNA Translation: AAC36129.1 BC003745 mRNA No translation available. |
CCDSi | CCDS39085.1 |
RefSeqi | NP_031865.2, NM_007839.3 |
Genome annotation databases
Ensembli | ENSMUST00000031061; ENSMUSP00000031061; ENSMUSG00000029169 |
GeneIDi | 13204 |
KEGGi | mmu:13204 |
UCSCi | uc008xkf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017153 mRNA Translation: AAC36129.1 BC003745 mRNA No translation available. |
CCDSi | CCDS39085.1 |
RefSeqi | NP_031865.2, NM_007839.3 |
3D structure databases
SMRi | O35286 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199083, 54 interactors |
CORUMi | O35286 |
DIPi | DIP-61875N |
IntActi | O35286, 7 interactors |
MINTi | O35286 |
STRINGi | 10090.ENSMUSP00000031061 |
PTM databases
iPTMneti | O35286 |
PhosphoSitePlusi | O35286 |
SwissPalmi | O35286 |
Proteomic databases
EPDi | O35286 |
jPOSTi | O35286 |
PaxDbi | O35286 |
PeptideAtlasi | O35286 |
PRIDEi | O35286 |
Protocols and materials databases
Antibodypediai | 10152, 155 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031061; ENSMUSP00000031061; ENSMUSG00000029169 |
GeneIDi | 13204 |
KEGGi | mmu:13204 |
UCSCi | uc008xkf.2, mouse |
Organism-specific databases
CTDi | 1665 |
MGIi | MGI:1099786, Dhx15 |
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
GeneTreei | ENSGT00940000155800 |
InParanoidi | O35286 |
OMAi | QWCVDFA |
OrthoDBi | 354219at2759 |
PhylomeDBi | O35286 |
TreeFami | TF105735 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 13204, 9 hits in 18 CRISPR screens |
ChiTaRSi | Dhx15, mouse |
PROi | PR:O35286 |
RNActi | O35286, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029169, Expressed in primitive streak and 287 other tissues |
Genevisiblei | O35286, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX15_MOUSE | |
Accessioni | O35286Primary (citable) accession number: O35286 Secondary accession number(s): Q99L91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 6, 2003 | |
Last modified: | February 10, 2021 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families