Batf

Functionⁱ

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8⁺ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8⁺ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8⁺ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.11 Publications

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to DNA damage stimulus
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22385964
cytokine production
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19578362
defense response to protozoan
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22992524
DNA damage response, signal transduction by p53 class mediator
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22385964
hematopoietic stem cell differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22385964
isotype switching
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20421391
- 21572431
lymphoid progenitor cell differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22385964
myeloid dendritic cell differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22992524
positive regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from mutant phenotypeⁱ
- 22983707
T-helper 17 cell differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20421391
- 21572431
T-helper 17 cell lineage commitment
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19578362
- 22983707
T-helper 2 cell differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20421391
- 21572431

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Differentiation, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Basic leucine zipper transcriptional factor ATF-like Alternative name(s): B-cell-activating transcription factor Short name: B-ATF
Gene namesⁱ	Name:Batf
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 12

Organism-specific databases

MGIⁱ	MGI:1859147 Batf

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice are fertile and healthy. They display a normal thymus, spleen and lymph node development, and normal CD4⁺ and CD8⁺ T-cell development. They also show normal development of natural killer T-cells, B-cells, and conventional and plasmacytoid dendritic cells. They however show defects in T-helper 17 cells (Th17) differentiation and are resistant to experimental autoimmune encephalomyelitis. Loss of follicular T-helper cells (TfH), as well as less production of antibodies with switched isotypes in B-cells is also observed.4 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	43	S → A: Decreased phosphorylation; when associated with A-48. 1 Publication	1
Mutagenesisⁱ	43	S → D: Retains the ability to dimerize with JUNB and localization in the nucleus but abolishes DNA-binding. 1 Publication	1
Mutagenesisⁱ	48	T → A: Decreased phosphorylation; when associated with A-43. 1 Publication	1
Mutagenesisⁱ	55	H → Q: Impairs interaction with IRF4 and the recruitment of IRF4 to AICE motifs, leading to defects in mediate differentiation of Th17 cells. Loss of function; when associated with A-56; D-63 and K-77. 2 Publications	1
Mutagenesisⁱ	56	L → A: Loss of function; when associated with Q-55; D-63 and K-77. 1 Publication	1
Mutagenesisⁱ	63	K → D: Retains 50% of activity; when associated with 69-Q-T-70 and K-77. Loss of function; when associated with Q-55; A-56 and K-77. 1 Publication	1
Mutagenesisⁱ	69 – 70	RK → QT: Retains 50% of activity; when associated with D-63 and K-77. 1 Publication	2
Mutagenesisⁱ	77	E → K: Does not affect interaction with IRF4 and ability to mediate differentiation of Th17 cells. Retains 50% of activity; when associated with D63 and 69-Q-T-70. Loss of function; when associated with Q-55; A-56 and D-63. 2 Publications	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000076596	1 – 125	Basic leucine zipper transcriptional factor ATF-likeAdd BLAST		125

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	43	Phosphoserine1 Publication		1
Modified residueⁱ	48	Phosphothreonine1 Publication		1

Post-translational modificationⁱ

Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	O35284
PaxDbⁱ	O35284
PRIDEⁱ	O35284

PTM databases

iPTMnetⁱ	O35284
PhosphoSitePlusⁱ	O35284

Expressionⁱ

Tissue specificityⁱ

Detected in postnatal and adult lymphoid tissues such as thymus, spleen and lymph nodes. In thymus most concentrated expression is found in the immediate cortical layer. Differentially expressed during T-cell development in thymus. Highly expressed in Th17, Th1 and Th2 cells and in activated B-cells.3 Publications

Inductionⁱ

Up-regulated by STAT3 in response to DNA damage. Induces by IL12 at late effector stage. Down-regulated by STAT5 in follicular T-helper cells (TfH).4 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000034266 Expressed in 102 organ(s), highest expression level in adult mammalian kidney
Genevisibleⁱ	O35284 MM

Interactionⁱ

Subunit structureⁱ

Heterodimer; mainly heterodimerizes with JUNB. The BATF-JUNB heterodimer interacts with IRF4 and IRF8. Interacts (via bZIP domain) with IRF4 and IRF8; the interaction is direct. Also forms heterodimers with JUN and JUND. Also Interacts with IFI35.5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Irf4	Q64287	7	EBI-6398523,EBI-6398485

With

Entry

#Exp.

IntAct

Notes

Irf4

Q64287

7

EBI-6398523,EBI-6398485

Protein-protein interaction databases

BioGridⁱ	207278, 1 interactor
IntActⁱ	O35284, 1 interactor
STRINGⁱ	10090.ENSMUSP00000040706

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O35284
SMRⁱ	O35284
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	26 – 89	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	28 – 50	Basic motifPROSITE-ProRule annotationAdd BLAST		23
Regionⁱ	54 – 75	Leucine-zipperPROSITE-ProRule annotationAdd BLAST		22

Sequence similaritiesⁱ

Belongs to the bZIP family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1414 Eukaryota ENOG4111CH5 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000016869
HOGENOMⁱ	HOG000236325
HOVERGENⁱ	HBG039173
InParanoidⁱ	O35284
KEGG Orthology (KO) More...KOⁱ	K09034
OMAⁱ	DSNDTSY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0YSM
PhylomeDBⁱ	O35284
TreeFamⁱ	TF332340

Family and domain databases

InterProⁱ	View protein in InterPro IPR000837 AP-1 IPR029820 BATF IPR004827 bZIP
PANTHERⁱ	PTHR23351 PTHR23351, 1 hit PTHR23351:SF14 PTHR23351:SF14, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00170 bZIP_1, 1 hit
PRINTSⁱ	PR00042 LEUZIPPRFOS
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O35284-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPHSSDSSDS SFSRSPPPGK QDSSDDVRKV QRREKNRIAA QKSRQRQTQK 
        60         70         80         90        100
ADTLHLESED LEKQNAALRK EIKQLTEELK YFTSVLSSHE PLCSVLASGT 
       110        120 
PSPPEVVYSA HAFHQPHISS PRFQP

Length:125

Mass (Da):14,065

Last modified:January 1, 1998 - v1

Checksum:ⁱA23B00D6E0827938

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF017021 mRNA Translation: AAB70251.1 AK018587 mRNA Translation: BAB31294.1 BC132410 mRNA Translation: AAI32411.1 BC132412 mRNA Translation: AAI32413.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26061.1
NCBI Reference Sequences More...RefSeqⁱ	NP_058047.1, NM_016767.2
UniGeneⁱ	Mm.6672

Genome annotation databases

Ensemblⁱ	ENSMUST00000040536; ENSMUSP00000040706; ENSMUSG00000034266
GeneIDⁱ	53314
KEGGⁱ	mmu:53314
UCSC genome browser More...UCSCⁱ	uc007ohd.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O35284	Basic leucine zipper transcriptional factor ATF-like		RAT		125	UniRef100_O35284

Protein	Similar proteins		Species	Score	Length	Source
O35284	Basic leucine zipper transcriptional factor ATF-like		RAT		125	UniRef90_O35284
Basic leucine zipper transcriptional factor ATF-like		BOVIN		125
Basic leucine zipper transcriptional factor ATF-like		HUMAN		125
Uncharacterized protein		CAVPO		126
Uncharacterized protein		MUSPF		125
+81

Protein	Similar proteins		Species	Score	Length	Source
O35284	Basic leucine zipper transcriptional factor ATF-like		RAT		125	UniRef50_O35284
Basic leucine zipper transcriptional factor ATF-like		BOVIN		125
Basic leucine zipper transcriptional factor ATF-like		HUMAN		125
Uncharacterized protein		CAVPO		126
basic leucine zipper transcriptional factor ATF-like		TARSY		125
+95

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF017021 mRNA Translation: AAB70251.1 AK018587 mRNA Translation: BAB31294.1 BC132410 mRNA Translation: AAI32411.1 BC132412 mRNA Translation: AAI32413.1
CCDSⁱ	CCDS26061.1
RefSeqⁱ	NP_058047.1, NM_016767.2
UniGeneⁱ	Mm.6672

3D structure databases

ProteinModelPortalⁱ	O35284
SMRⁱ	O35284
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	207278, 1 interactor
IntActⁱ	O35284, 1 interactor
STRINGⁱ	10090.ENSMUSP00000040706

PTM databases

iPTMnetⁱ	O35284
PhosphoSitePlusⁱ	O35284

Proteomic databases

EPDⁱ	O35284
PaxDbⁱ	O35284
PRIDEⁱ	O35284

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000040536; ENSMUSP00000040706; ENSMUSG00000034266
GeneIDⁱ	53314
KEGGⁱ	mmu:53314
UCSCⁱ	uc007ohd.1 mouse

Organism-specific databases

CTDⁱ	10538
MGIⁱ	MGI:1859147 Batf

Phylogenomic databases

eggNOGⁱ	KOG1414 Eukaryota ENOG4111CH5 LUCA
GeneTreeⁱ	ENSGT00390000016869
HOGENOMⁱ	HOG000236325
HOVERGENⁱ	HBG039173
InParanoidⁱ	O35284
KOⁱ	K09034
OMAⁱ	DSNDTSY
OrthoDBⁱ	EOG091G0YSM
PhylomeDBⁱ	O35284
TreeFamⁱ	TF332340

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O35284
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000034266 Expressed in 102 organ(s), highest expression level in adult mammalian kidney
Genevisibleⁱ	O35284 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR000837 AP-1 IPR029820 BATF IPR004827 bZIP
PANTHERⁱ	PTHR23351 PTHR23351, 1 hit PTHR23351:SF14 PTHR23351:SF14, 1 hit
Pfamⁱ	View protein in Pfam PF00170 bZIP_1, 1 hit
PRINTSⁱ	PR00042 LEUZIPPRFOS
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BATF_MOUSE
Accessionⁱ	O35284Primary (citable) accession number: O35284 Secondary accession number(s): A2RT86
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
	Last sequence update:	January 1, 1998
	Last modified:	September 12, 2018
	This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O35284 (BATF_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Basic leucine zipper transcriptional factor ATF-like

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ