UniProtKB - O35284 (BATF_MOUSE)
Protein
Basic leucine zipper transcriptional factor ATF-like
Gene
Batf
Organism
Mus musculus (Mouse)
Status
Functioni
AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8+ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8+ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.11 Publications
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- defense response to protozoan Source: UniProtKB
- DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
- hematopoietic stem cell differentiation Source: UniProtKB
- isotype switching Source: UniProtKB
- lymphoid progenitor cell differentiation Source: UniProtKB
- myeloid dendritic cell differentiation Source: UniProtKB
- positive regulation of cytokine production Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
- T-helper 17 cell differentiation Source: UniProtKB
- T-helper 17 cell lineage commitment Source: UniProtKB
- T-helper 2 cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Basic leucine zipper transcriptional factor ATF-likeAlternative name(s): B-cell-activating transcription factor Short name: B-ATF |
Gene namesi | Name:Batf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859147, Batf |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mice are fertile and healthy. They display a normal thymus, spleen and lymph node development, and normal CD4+ and CD8+ T-cell development. They also show normal development of natural killer T-cells, B-cells, and conventional and plasmacytoid dendritic cells. They however show defects in T-helper 17 cells (Th17) differentiation and are resistant to experimental autoimmune encephalomyelitis. Loss of follicular T-helper cells (TfH), as well as less production of antibodies with switched isotypes in B-cells is also observed.4 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 | S → A: Decreased phosphorylation; when associated with A-48. 1 Publication | 1 | |
Mutagenesisi | 43 | S → D: Retains the ability to dimerize with JUNB and localization in the nucleus but abolishes DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 48 | T → A: Decreased phosphorylation; when associated with A-43. 1 Publication | 1 | |
Mutagenesisi | 55 | H → Q: Impairs interaction with IRF4 and the recruitment of IRF4 to AICE motifs, leading to defects in mediate differentiation of Th17 cells. Loss of function; when associated with A-56; D-63 and K-77. 2 Publications | 1 | |
Mutagenesisi | 56 | L → A: Loss of function; when associated with Q-55; D-63 and K-77. 1 Publication | 1 | |
Mutagenesisi | 63 | K → D: Retains 50% of activity; when associated with 69-Q-T-70 and K-77. Loss of function; when associated with Q-55; A-56 and K-77. 1 Publication | 1 | |
Mutagenesisi | 69 – 70 | RK → QT: Retains 50% of activity; when associated with D-63 and K-77. 1 Publication | 2 | |
Mutagenesisi | 77 | E → K: Does not affect interaction with IRF4 and ability to mediate differentiation of Th17 cells. Retains 50% of activity; when associated with D63 and 69-Q-T-70. Loss of function; when associated with Q-55; A-56 and D-63. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076596 | 1 – 125 | Basic leucine zipper transcriptional factor ATF-likeAdd BLAST | 125 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 43 | Phosphoserine1 Publication | 1 | |
Modified residuei | 48 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O35284 |
PaxDbi | O35284 |
PRIDEi | O35284 |
ProteomicsDBi | 273652 |
PTM databases
iPTMneti | O35284 |
PhosphoSitePlusi | O35284 |
Expressioni
Tissue specificityi
Detected in postnatal and adult lymphoid tissues such as thymus, spleen and lymph nodes. In thymus most concentrated expression is found in the immediate cortical layer. Differentially expressed during T-cell development in thymus. Highly expressed in Th17, Th1 and Th2 cells and in activated B-cells.3 Publications
Inductioni
Up-regulated by STAT3 in response to DNA damage. Induces by IL12 at late effector stage. Down-regulated by STAT5 in follicular T-helper cells (TfH).4 Publications
Gene expression databases
Bgeei | ENSMUSG00000034266, Expressed in adult mammalian kidney and 128 other tissues |
Genevisiblei | O35284, MM |
Interactioni
Subunit structurei
Heterodimer; mainly heterodimerizes with JUNB. The BATF-JUNB heterodimer interacts with IRF4 and IRF8.
Interacts (via bZIP domain) with IRF4 and IRF8; the interaction is direct. Also forms heterodimers with JUN and JUND.
Interacts with IFI35.
5 PublicationsBinary interactionsi
O35284
With | #Exp. | IntAct |
---|---|---|
Irf4 [Q64287] | 7 | EBI-6398523,EBI-6398485 |
Protein-protein interaction databases
BioGRIDi | 207278, 1 interactor |
IntActi | O35284, 1 interactor |
STRINGi | 10090.ENSMUSP00000040706 |
Miscellaneous databases
RNActi | O35284, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 89 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 50 | Basic motifPROSITE-ProRule annotationAdd BLAST | 23 | |
Regioni | 54 – 75 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the bZIP family.Curated
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159745 |
HOGENOMi | CLU_088612_4_0_1 |
InParanoidi | O35284 |
OMAi | SYHHQHI |
OrthoDBi | 1544557at2759 |
PhylomeDBi | O35284 |
TreeFami | TF332340 |
Family and domain databases
InterProi | View protein in InterPro IPR000837, AP-1 IPR029820, BATF IPR004827, bZIP |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF14, PTHR23351:SF14, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
O35284-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPHSSDSSDS SFSRSPPPGK QDSSDDVRKV QRREKNRIAA QKSRQRQTQK
60 70 80 90 100
ADTLHLESED LEKQNAALRK EIKQLTEELK YFTSVLSSHE PLCSVLASGT
110 120
PSPPEVVYSA HAFHQPHISS PRFQP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017021 mRNA Translation: AAB70251.1 AK018587 mRNA Translation: BAB31294.1 BC132410 mRNA Translation: AAI32411.1 BC132412 mRNA Translation: AAI32413.1 |
CCDSi | CCDS26061.1 |
RefSeqi | NP_058047.1, NM_016767.2 |
Genome annotation databases
Ensembli | ENSMUST00000040536; ENSMUSP00000040706; ENSMUSG00000034266 |
GeneIDi | 53314 |
KEGGi | mmu:53314 |
UCSCi | uc007ohd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017021 mRNA Translation: AAB70251.1 AK018587 mRNA Translation: BAB31294.1 BC132410 mRNA Translation: AAI32411.1 BC132412 mRNA Translation: AAI32413.1 |
CCDSi | CCDS26061.1 |
RefSeqi | NP_058047.1, NM_016767.2 |
3D structure databases
SMRi | O35284 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207278, 1 interactor |
IntActi | O35284, 1 interactor |
STRINGi | 10090.ENSMUSP00000040706 |
PTM databases
iPTMneti | O35284 |
PhosphoSitePlusi | O35284 |
Proteomic databases
EPDi | O35284 |
PaxDbi | O35284 |
PRIDEi | O35284 |
ProteomicsDBi | 273652 |
Protocols and materials databases
Antibodypediai | 25838, 396 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040536; ENSMUSP00000040706; ENSMUSG00000034266 |
GeneIDi | 53314 |
KEGGi | mmu:53314 |
UCSCi | uc007ohd.1, mouse |
Organism-specific databases
CTDi | 10538 |
MGIi | MGI:1859147, Batf |
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159745 |
HOGENOMi | CLU_088612_4_0_1 |
InParanoidi | O35284 |
OMAi | SYHHQHI |
OrthoDBi | 1544557at2759 |
PhylomeDBi | O35284 |
TreeFami | TF332340 |
Miscellaneous databases
BioGRID-ORCSi | 53314, 0 hits in 52 CRISPR screens |
PROi | PR:O35284 |
RNActi | O35284, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034266, Expressed in adult mammalian kidney and 128 other tissues |
Genevisiblei | O35284, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000837, AP-1 IPR029820, BATF IPR004827, bZIP |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF14, PTHR23351:SF14, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BATF_MOUSE | |
Accessioni | O35284Primary (citable) accession number: O35284 Secondary accession number(s): A2RT86 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families