UniProtKB - O35261 (E2F3_MOUSE)

BLAST

>sp|O35261|E2F3_MOUSE Transcription factor E2F3 OS=Mus musculus OX=10090 GN=E2f3 PE=1 SV=2
MRKGIQPALEQYLVTAGGGEGAAVVAAAAAASMDKRALLASPGFAAAAAPGTYIQILTTN
PSTTSCATSLQSGALTAGPLLPSVPGTEPAASSLYTTPQGPSSRVGLLQQPPAPGRGGGG
GPPAKRRLELGESGHQYLSDGLKTPKGKGRAALRSPDSPKTPKSPSEKTRYDTSLGLLTK
KFIQLLSQSPDGVLDLNKAAEVLKVQKRRIYDITNVLEGIHLIKKKSKNNVQWMGCSLSE
DGGMLAQCQGLSKEVTELSQEEKKLDELIQSCTLDLKLLTEDSENQRLAYVTYQDIRKIS
GLKDQTVIVVKAPPETRLEVPDSIESLQIHLASTQGPIEVYLCPEETETHRPMKTNNQDH
NGNIPKPTSKDLASNNSGHSDCSVSTANLSPLASPANLLQQTEDQIPSNLEGPFVNLLPP
LLQEDYLLSLGEEEGISDLFDAYDLEKLPLVEDFMCS

Align
Format
Add to basket Added to basket
History
Entry version 146 (07 Nov 2018)
Sequence version 2 (30 Aug 2005)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Transcription factor E2F3

Gene

E2f3

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812).1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	147 – 237	Sequence analysisAdd BLAST		91

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- 11980909
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein dimerization activity Source: InterPro
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
proximal promoter sequence-specific DNA binding Source: MGI
RNA polymerase II regulatory region sequence-specific DNA binding Source: Ensembl
sequence-specific DNA binding Source: MGI
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
negative regulation of fat cell proliferation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20176812
negative regulation of transcription by RNA polymerase II
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
positive regulation of cell proliferation
Source: MGI
Inferred from direct assayⁱ
- 12893818
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 12893818
positive regulation of transcription by RNA polymerase II Source: MGI
positive regulation of vascular associated smooth muscle cell apoptotic process Source: MGI
regulation of cell cycle
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 11980909

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-68689 CDC6 association with the ORC:origin complex R-MMU-68911 G2 Phase R-MMU-69231 Cyclin D associated events in G1

Reactomeⁱ

R-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68911 G2 Phase
R-MMU-69231 Cyclin D associated events in G1

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor E2F3 Short name: E2F-3
Gene namesⁱ	Name:E2f3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 13

Organism-specific databases

MGIⁱ	MGI:1096340 E2f3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000219467	1 – 457	Transcription factor E2F3Add BLAST		457

Proteomic databases

EPDⁱ	O35261
PaxDbⁱ	O35261
PeptideAtlas More...PeptideAtlasⁱ	O35261
PRIDEⁱ	O35261

PTM databases

iPTMnetⁱ	O35261
PhosphoSitePlusⁱ	O35261

Expressionⁱ

Developmental stageⁱ

In the developing nervous system, high levels expressed in both ventral and dorsal regions of the spinal cord from 13.5 dpc. Also expressed in dorsal root and cranial ganglia in 11.5-18.5 dpc embryos. Only low levels of expression in developing brain. In the developing retina (15.5 dpc), expression of E2F3 is localized to the ganglion cell layer. In other developing tissues, expressed in liver, lung and heart. Weak expression in developing kidney and skeletal muscle. Absent from the developing choroid plexus, thymus and developing skin. Low levels of expression in the developing intestinal epithelium and mesenchyme in 12.5-18.5 dpc embryos.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000016477 Expressed in 275 organ(s), highest expression level in floor plate of midbrain
CleanExⁱ	MM_E2F3
ExpressionAtlasⁱ	O35261 baseline and differential
Genevisibleⁱ	O35261 MM

Interactionⁱ

Subunit structureⁱ

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199351, 2 interactors
CORUMⁱ	O35261
Database of interacting proteins More...DIPⁱ	DIP-59314N
IntActⁱ	O35261, 1 interactor
STRINGⁱ	10090.ENSMUSP00000100012

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O35261
SMRⁱ	O35261
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	96 – 145	Cyclin A/CDK2 bindingSequence analysisAdd BLAST	50
Regionⁱ	196 – 217	Leucine-zipperAdd BLAST	22
Regionⁱ	238 – 329	DimerizationSequence analysisAdd BLAST	92
Regionⁱ	383 – 457	TransactivationSequence analysisAdd BLAST	75
Regionⁱ	424 – 441	Retinoblastoma protein bindingSequence analysisAdd BLAST	18

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	201 – 237	DEF boxAdd BLAST		37

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	22 – 49	Ala-richAdd BLAST		28
Compositional biasⁱ	115 – 121	Poly-Gly		7

Sequence similaritiesⁱ

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2577 Eukaryota ENOG410XNYI LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074403
HOGENOMⁱ	HOG000232045
HOVERGENⁱ	HBG002227
InParanoidⁱ	O35261
KEGG Orthology (KO) More...KOⁱ	K06620
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G087U
PhylomeDBⁱ	O35261
TreeFamⁱ	TF105566

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd14660 E2F_DD, 1 hit
Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR015633 E2F IPR037241 E2F-DP_heterodim IPR032198 E2F_CC-MB IPR003316 E2F_WHTH_DNA-bd_dom IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR12081 PTHR12081, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16421 E2F_CC-MB, 1 hit PF02319 E2F_TDP, 1 hit
SMARTⁱ	View protein in SMART SM01372 E2F_TDP, 1 hit
SUPFAMⁱ	SSF144074 SSF144074, 1 hit SSF46785 SSF46785, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

O35261-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP 
        60         70         80         90        100
GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG 
       110        120        130        140        150
PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR 
       160        170        180        190        200
AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA 
       210        220        230        240        250
EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG 
       260        270        280        290        300
LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS 
       310        320        330        340        350
GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH 
       360        370        380        390        400
RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ 
       410        420        430        440        450
QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL 

VEDFMCS

Length:457

Mass (Da):48,757

Last modified:August 30, 2005 - v2

Checksum:ⁱ076AC9F360101075

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q3TMJ9	Q3TMJ9_MOUSE	Transcription factor E2F3 Transcription factor E2F3	E2f3	334	Annotation score:
A0A1Y7VML1	A0A1Y7VML1_MOUSE	Transcription factor E2F3 Transcription factor E2F3	E2f3	340	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	102 – 109	SSRVGLLQ → CSSPTLLE in AAB71671 (PubMed:9376316).Curated		8
Sequence conflictⁱ	309	V → L in AAB71671 (PubMed:9376316).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL513025 Genomic DNA Translation: CAI24679.1 AF015948 mRNA Translation: AAB71671.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26413.1
NCBI Reference Sequences More...RefSeqⁱ	NP_034223.1, NM_010093.3
UniGeneⁱ	Mm.268356

Genome annotation databases

Ensemblⁱ	ENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477
GeneIDⁱ	13557
KEGGⁱ	mmu:13557
UCSC genome browser More...UCSCⁱ	uc007pyq.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O35261	Uncharacterized protein		MOUSE		457	UniRef100_O35261

Protein	Similar proteins		Species	Score	Length	Source
O35261	Transcription factor E2F3		HUMAN		465	UniRef90_O00716
transcription factor E2F3		LIPVE		469
transcription factor E2F3 isoform X1		ODORO		468
Transcription factor E2F3 (Fragment)		NEOVI		296
Uncharacterized protein		MACMU		467
+97

Protein	Similar proteins		Species	Score	Length	Source
O35261	Transcription factor E2F3		HUMAN		465	UniRef50_O00716
transcription factor E2F3		LIPVE		469
transcription factor E2F3 isoform X1		ODORO		468
Transcription factor E2F3 (Fragment)		NEOVI		296
Uncharacterized protein		MACMU		467
+118

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL513025 Genomic DNA Translation: CAI24679.1 AF015948 mRNA Translation: AAB71671.1
CCDSⁱ	CCDS26413.1
RefSeqⁱ	NP_034223.1, NM_010093.3
UniGeneⁱ	Mm.268356

3D structure databases

ProteinModelPortalⁱ	O35261
SMRⁱ	O35261
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199351, 2 interactors
CORUMⁱ	O35261
DIPⁱ	DIP-59314N
IntActⁱ	O35261, 1 interactor
STRINGⁱ	10090.ENSMUSP00000100012

PTM databases

iPTMnetⁱ	O35261
PhosphoSitePlusⁱ	O35261

Proteomic databases

EPDⁱ	O35261
PaxDbⁱ	O35261
PeptideAtlasⁱ	O35261
PRIDEⁱ	O35261

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	13557
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477
GeneIDⁱ	13557
KEGGⁱ	mmu:13557
UCSCⁱ	uc007pyq.2 mouse

Organism-specific databases

CTDⁱ	1871
MGIⁱ	MGI:1096340 E2f3

Phylogenomic databases

eggNOGⁱ	KOG2577 Eukaryota ENOG410XNYI LUCA
GeneTreeⁱ	ENSGT00550000074403
HOGENOMⁱ	HOG000232045
HOVERGENⁱ	HBG002227
InParanoidⁱ	O35261
KOⁱ	K06620
OrthoDBⁱ	EOG091G087U
PhylomeDBⁱ	O35261
TreeFamⁱ	TF105566

Enzyme and pathway databases

Reactomeⁱ	R-MMU-68689 CDC6 association with the ORC:origin complex R-MMU-68911 G2 Phase R-MMU-69231 Cyclin D associated events in G1

Reactomeⁱ

R-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68911 G2 Phase
R-MMU-69231 Cyclin D associated events in G1

Miscellaneous databases

ChiTaRSⁱ	E2f3 mouse
Protein Ontology More...PROⁱ	PR:O35261
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000016477 Expressed in 275 organ(s), highest expression level in floor plate of midbrain
CleanExⁱ	MM_E2F3
ExpressionAtlasⁱ	O35261 baseline and differential
Genevisibleⁱ	O35261 MM

Family and domain databases

CDDⁱ	cd14660 E2F_DD, 1 hit
Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR015633 E2F IPR037241 E2F-DP_heterodim IPR032198 E2F_CC-MB IPR003316 E2F_WHTH_DNA-bd_dom IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR12081 PTHR12081, 1 hit
Pfamⁱ	View protein in Pfam PF16421 E2F_CC-MB, 1 hit PF02319 E2F_TDP, 1 hit
SMARTⁱ	View protein in SMART SM01372 E2F_TDP, 1 hit
SUPFAMⁱ	SSF144074 SSF144074, 1 hit SSF46785 SSF46785, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	E2F3_MOUSE
Accessionⁱ	O35261Primary (citable) accession number: O35261 Secondary accession number(s): Q5T0I6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	August 30, 2005
	Last modified:	November 7, 2018
	This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O35261 (E2F3_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Transcription factor E2F3

E2f3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ