UniProtKB - O35261 (E2F3_MOUSE)
Protein
Transcription factor E2F3
Gene
E2f3
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812).1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 147 – 237 | Sequence analysisAdd BLAST | 91 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- G1/S transition of mitotic cell cycle Source: MGI
- negative regulation of fat cell proliferation Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of vascular associated smooth muscle cell apoptotic process Source: MGI
- protein import into nucleus Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68911, G2 Phase R-MMU-69231, Cyclin D associated events in G1 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor E2F3Short name: E2F-3 |
Gene namesi | Name:E2f3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096340, E2f3 |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219467 | 1 – 457 | Transcription factor E2F3Add BLAST | 457 |
Proteomic databases
EPDi | O35261 |
PaxDbi | O35261 |
PeptideAtlasi | O35261 |
PRIDEi | O35261 |
ProteomicsDBi | 277700 |
PTM databases
iPTMneti | O35261 |
PhosphoSitePlusi | O35261 |
Expressioni
Developmental stagei
In the developing nervous system, high levels expressed in both ventral and dorsal regions of the spinal cord from 13.5 dpc. Also expressed in dorsal root and cranial ganglia in 11.5-18.5 dpc embryos. Only low levels of expression in developing brain. In the developing retina (15.5 dpc), expression of E2F3 is localized to the ganglion cell layer. In other developing tissues, expressed in liver, lung and heart. Weak expression in developing kidney and skeletal muscle. Absent from the developing choroid plexus, thymus and developing skin. Low levels of expression in the developing intestinal epithelium and mesenchyme in 12.5-18.5 dpc embryos.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000016477, Expressed in adult mammalian kidney and 285 other tissues |
Genevisiblei | O35261, MM |
Interactioni
Subunit structurei
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites.
Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.
1 PublicationGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 199351, 2 interactors |
CORUMi | O35261 |
DIPi | DIP-59314N |
IntActi | O35261, 1 interactor |
STRINGi | 10090.ENSMUSP00000100012 |
Miscellaneous databases
RNActi | O35261, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 145 | Cyclin A/CDK2 bindingSequence analysisAdd BLAST | 50 | |
Regioni | 196 – 217 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 238 – 329 | DimerizationSequence analysisAdd BLAST | 92 | |
Regioni | 383 – 457 | TransactivationSequence analysisAdd BLAST | 75 | |
Regioni | 424 – 441 | Retinoblastoma protein bindingSequence analysisAdd BLAST | 18 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 201 – 237 | DEF boxAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 49 | Ala-richAdd BLAST | 28 | |
Compositional biasi | 115 – 121 | Poly-Gly | 7 |
Sequence similaritiesi
Belongs to the E2F/DP family.Curated
Phylogenomic databases
eggNOGi | KOG2577, Eukaryota |
GeneTreei | ENSGT00940000155115 |
HOGENOMi | CLU_032091_0_0_1 |
InParanoidi | O35261 |
OrthoDBi | 1087250at2759 |
PhylomeDBi | O35261 |
TreeFami | TF105566 |
Family and domain databases
CDDi | cd14660, E2F_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR015633, E2F IPR037241, E2F-DP_heterodim IPR032198, E2F_CC-MB IPR003316, E2F_WHTH_DNA-bd_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12081, PTHR12081, 1 hit |
Pfami | View protein in Pfam PF16421, E2F_CC-MB, 1 hit PF02319, E2F_TDP, 1 hit |
SMARTi | View protein in SMART SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O35261-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP
60 70 80 90 100
GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG
110 120 130 140 150
PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR
160 170 180 190 200
AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA
210 220 230 240 250
EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG
260 270 280 290 300
LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS
310 320 330 340 350
GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH
360 370 380 390 400
RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ
410 420 430 440 450
QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL
VEDFMCS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TMJ9 | Q3TMJ9_MOUSE | Transcription factor E2F3 | E2f3 | 334 | Annotation score: | ||
A0A1Y7VML1 | A0A1Y7VML1_MOUSE | Transcription factor E2F3 | E2f3 | 340 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 – 109 | SSRVGLLQ → CSSPTLLE in AAB71671 (PubMed:9376316).Curated | 8 | |
Sequence conflicti | 309 | V → L in AAB71671 (PubMed:9376316).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513025 Genomic DNA Translation: CAI24679.1 AF015948 mRNA Translation: AAB71671.1 |
CCDSi | CCDS26413.1 |
RefSeqi | NP_034223.1, NM_010093.3 |
Genome annotation databases
Ensembli | ENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477 |
GeneIDi | 13557 |
KEGGi | mmu:13557 |
UCSCi | uc007pyq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513025 Genomic DNA Translation: CAI24679.1 AF015948 mRNA Translation: AAB71671.1 |
CCDSi | CCDS26413.1 |
RefSeqi | NP_034223.1, NM_010093.3 |
3D structure databases
SMRi | O35261 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199351, 2 interactors |
CORUMi | O35261 |
DIPi | DIP-59314N |
IntActi | O35261, 1 interactor |
STRINGi | 10090.ENSMUSP00000100012 |
PTM databases
iPTMneti | O35261 |
PhosphoSitePlusi | O35261 |
Proteomic databases
EPDi | O35261 |
PaxDbi | O35261 |
PeptideAtlasi | O35261 |
PRIDEi | O35261 |
ProteomicsDBi | 277700 |
Protocols and materials databases
Antibodypediai | 10438, 341 antibodies |
DNASUi | 13557 |
Genome annotation databases
Ensembli | ENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477 |
GeneIDi | 13557 |
KEGGi | mmu:13557 |
UCSCi | uc007pyq.2, mouse |
Organism-specific databases
CTDi | 1871 |
MGIi | MGI:1096340, E2f3 |
Phylogenomic databases
eggNOGi | KOG2577, Eukaryota |
GeneTreei | ENSGT00940000155115 |
HOGENOMi | CLU_032091_0_0_1 |
InParanoidi | O35261 |
OrthoDBi | 1087250at2759 |
PhylomeDBi | O35261 |
TreeFami | TF105566 |
Enzyme and pathway databases
Reactomei | R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68911, G2 Phase R-MMU-69231, Cyclin D associated events in G1 |
Miscellaneous databases
BioGRID-ORCSi | 13557, 3 hits in 52 CRISPR screens |
ChiTaRSi | E2f3, mouse |
PROi | PR:O35261 |
RNActi | O35261, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000016477, Expressed in adult mammalian kidney and 285 other tissues |
Genevisiblei | O35261, MM |
Family and domain databases
CDDi | cd14660, E2F_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR015633, E2F IPR037241, E2F-DP_heterodim IPR032198, E2F_CC-MB IPR003316, E2F_WHTH_DNA-bd_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12081, PTHR12081, 1 hit |
Pfami | View protein in Pfam PF16421, E2F_CC-MB, 1 hit PF02319, E2F_TDP, 1 hit |
SMARTi | View protein in SMART SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | E2F3_MOUSE | |
Accessioni | O35261Primary (citable) accession number: O35261 Secondary accession number(s): Q5T0I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 30, 2005 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families