UniProtKB - O35228 (IL27B_MOUSE)
Interleukin-27 subunit beta
Ebi3
Functioni
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- cytokine binding Source: GO_Central
- cytokine receptor activity Source: MGI
- interleukin-27 receptor binding Source: MGI
- peptide hormone binding Source: GO_Central
GO - Biological processi
- cytokine-mediated signaling pathway Source: GO_Central
- leptin-mediated signaling pathway Source: GO_Central
- T cell proliferation Source: CACAO
Keywordsi
Molecular function | Cytokine |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin-27 subunit betaShort name: IL-27 subunit beta Short name: IL-27B Alternative name(s): Epstein-Barr virus-induced gene 3 protein homolog |
Gene namesi | Name:Ebi3 Synonyms:Il27b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354171, Ebi3 |
VEuPathDBi | HostDB:ENSMUSG00000003206 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB-KW
Plasma Membrane
- external side of plasma membrane Source: GO_Central
Other locations
- receptor complex Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000010938 | 19 – 228 | Interleukin-27 subunit betaAdd BLAST | 210 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 54 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | O35228 |
PeptideAtlasi | O35228 |
PRIDEi | O35228 |
ProteomicsDBi | 269393 |
PTM databases
GlyGeni | O35228, 2 sites |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000003206, Expressed in granulocyte and 151 other tissues |
ExpressionAtlasi | O35228, baseline and differential |
Genevisiblei | O35228, MM |
Interactioni
Subunit structurei
Heterodimer with IL27/IL27A; not disulfide-linked. This heterodimer is known as interleukin IL-27. Heterodimer with IL12A; not disulfide-linked. This heterodimer is known as interleukin IL-35.
Interacts with SQSTM1.
By similarityBinary interactionsi
O35228
With | #Exp. | IntAct |
---|---|---|
Il12a [P43431] | 2 | EBI-3862967,EBI-3862959 |
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- cytokine binding Source: GO_Central
- interleukin-27 receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206044, 1 interactor |
DIPi | DIP-60364N |
IntActi | O35228, 1 interactor |
STRINGi | 10090.ENSMUSP00000003274 |
Miscellaneous databases
RNActi | O35228, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 124 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 99 | |
Domaini | 127 – 224 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 98 |
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502RXJ4, Eukaryota |
GeneTreei | ENSGT00940000160050 |
HOGENOMi | CLU_047259_2_0_1 |
InParanoidi | O35228 |
OMAi | TSCTIAD |
OrthoDBi | 857138at2759 |
PhylomeDBi | O35228 |
TreeFami | TF331210 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR003530, Hematopoietin_rcpt_L_F3_CS IPR013783, Ig-like_fold IPR015373, Interferon/interleukin_rcp_dom |
Pfami | View protein in Pfam PF09294, Interfer-bind, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit |
SUPFAMi | SSF49265, SSF49265, 2 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 2 hits PS01354, HEMATOPO_REC_L_F3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL
60 70 80 90 100
QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP
110 120 130 140 150
YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP
160 170 180 190 200
ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ
210 220
VSAQDLTDYG KPSDWSLPGQ VESAPHKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013114 mRNA Translation: AAB67115.1 BC008209 mRNA Translation: AAH08209.1 |
CCDSi | CCDS28888.1 |
RefSeqi | NP_056581.1, NM_015766.2 |
Genome annotation databases
Ensembli | ENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206 |
GeneIDi | 50498 |
KEGGi | mmu:50498 |
UCSCi | uc008daj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013114 mRNA Translation: AAB67115.1 BC008209 mRNA Translation: AAH08209.1 |
CCDSi | CCDS28888.1 |
RefSeqi | NP_056581.1, NM_015766.2 |
3D structure databases
AlphaFoldDBi | O35228 |
SMRi | O35228 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206044, 1 interactor |
DIPi | DIP-60364N |
IntActi | O35228, 1 interactor |
STRINGi | 10090.ENSMUSP00000003274 |
PTM databases
GlyGeni | O35228, 2 sites |
Proteomic databases
PaxDbi | O35228 |
PeptideAtlasi | O35228 |
PRIDEi | O35228 |
ProteomicsDBi | 269393 |
Protocols and materials databases
Antibodypediai | 23527, 1090 antibodies from 39 providers |
DNASUi | 50498 |
Genome annotation databases
Ensembli | ENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206 |
GeneIDi | 50498 |
KEGGi | mmu:50498 |
UCSCi | uc008daj.1, mouse |
Organism-specific databases
CTDi | 10148 |
MGIi | MGI:1354171, Ebi3 |
VEuPathDBi | HostDB:ENSMUSG00000003206 |
Phylogenomic databases
eggNOGi | ENOG502RXJ4, Eukaryota |
GeneTreei | ENSGT00940000160050 |
HOGENOMi | CLU_047259_2_0_1 |
InParanoidi | O35228 |
OMAi | TSCTIAD |
OrthoDBi | 857138at2759 |
PhylomeDBi | O35228 |
TreeFami | TF331210 |
Miscellaneous databases
BioGRID-ORCSi | 50498, 3 hits in 72 CRISPR screens |
PROi | PR:O35228 |
RNActi | O35228, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003206, Expressed in granulocyte and 151 other tissues |
ExpressionAtlasi | O35228, baseline and differential |
Genevisiblei | O35228, MM |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR003530, Hematopoietin_rcpt_L_F3_CS IPR013783, Ig-like_fold IPR015373, Interferon/interleukin_rcp_dom |
Pfami | View protein in Pfam PF09294, Interfer-bind, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit |
SUPFAMi | SSF49265, SSF49265, 2 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 2 hits PS01354, HEMATOPO_REC_L_F3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IL27B_MOUSE | |
Accessioni | O35228Primary (citable) accession number: O35228 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families